口蹄疫病毒A型竞争ELISA抗体检测试剂盒在流行病学调查中的应用     

陈小丽  邵钰  仲焕香  宫枫举  汤荣福  孙学强  张志 《中国动物检疫》2021,38(2):104-107

为评价口蹄疫病毒A型竞争ELISA(cELISA)抗体检测试剂盒在流行病学调查中的应用前景,对2017年从福建省三明市采集的336份黄牛、奶牛、羊和猪血清样品,用A型cELISA抗体检测试剂盒进行抗体检测。结果显示,92份黄牛血清、92份羊血清、92份猪血清、60份奶牛血清的A型抗体阳性率分别为13.04%、11.96%、20.65%、86.67%。从上述4种血清中,各挑选10份血清(阴性、阳性各5份)共40份,采用口蹄疫病毒液相阻断ELISA(LPB-ELISA)抗体检测试剂盒进行验证。结果显示:cELISA检测为阳性的20份血清中,用LPB-ELISA检出阳性19份;cELISA检测为阴性的20份血清中,用LPB-ELISA检出阴性17份;两种方法的κ值为0.8,总符合率为90.00%。结果表明,A型cELISA试剂盒与LPB-ELISA试剂盒的符合率和一致性均较高,可用于口蹄疫流行病学调查和血清学监测。  相似文献   

基于CPLD的漏播检测装置的设计与测试     

赵艳华  龚丽农  尚书旗  王延耀  于艳 《农机化研究》2015,(9):126-130

针对现有漏播检测装置并行性差、响应速度慢等不足,提出一种新型的漏播检测装置。该装置采用复杂可编程逻辑器件(CPLD)作为检测控制核心,能够同时提供8路落种检测,适用于大型精密穴播机械。该检测装置采用光电器件作为落种传感器,响应快速准确。6对红外对管采用特殊方式排列在排种管的相对两侧,避免光线干涉,提高检测精度。该装置适用于检测直径3mm以上种子(如麦粒、黄豆、玉米粒等),能够配合上位机GPS精确定位漏播位置,为漏播后的补种修正提供位置和数目信息。手工落种测试时,对小麦、黄豆、玉米种子的检出率可达100%。将该装置在播种试验台上进行实验,统计自动落种数目,精度可达95%以上;对单粒漏播进行报警统计,精度可达80%以上。改进试验台实验条件,以自适应方式运行可以使装置的检测精度进一步提升。  相似文献   

Evaluating and Comparing the Natural Cell Structure and Dimensions of Honey Bee Comb Cells of Chinese Bee,Apis cerana cerana (Hymenoptera: Apidae) and Italian Bee,Apis mellifera ligustica (Hymenoptera: Apidae)     

Shunhua Yang  Shangkao Deng  Haiou Kuang  Danyin Zhou  Xueyang Gong  Kun Dong 《Journal of insect science (Online)》2021,21(4)

The hexagonal structure of the honey bee comb cell has been the source of many studies attempting to understand its structure and function. In the storage area of the comb, only honey is stored and no brood is reared. We predicted that honey bees may construct different hexagonal cells for brood rearing and honey storage. We used quantitative analyses to evaluate the structure and function of the natural comb cell in the Chinese bee, Apis cerana cerana and the Italian bee, A. mellifera ligustica. We made cell molds using a crystal glue solution and measured the structure and inclination of cells. We found that the comb cells of A. c. cerana had both upward-sloping and downward-sloping cells; while the A. m. ligustica cells all tilted upwards. Interestingly, the cells did not conform to the regular hexagonal prism structure and showed irregular diameter sizes. In both species, comb cells also were differentiated into worker, drone and honey cells, differing in their diameter and depth. This study revealed unique differences in the structure and function of comb cells and showed that honey bees design their cells with precise engineering to increase storage capacity, and to create adequate growing room for their brood.  相似文献   

干旱胁迫对3种冷季型草坪草光谱反射率及生理特征的影响     

赵志军  单贵莲  段新慧  姜华  任健  陈功  初晓辉 《草原与草坪》2016,(6):23-29

为探讨高光谱分析技术在草坪草水分监测上的应用,以草地早熟禾、高羊茅、多年生黑麦草为试验材料,测定了干旱胁迫下3种冷季型草坪草生理指标及光谱反射率。结果表明:随着干旱胁迫程度的加深,供试草坪草叶片相对含水量和叶绿素含量显著下降(P0.05),丙二醛含量、脯氨酸含量及敏感波段的(550和760 nm)的光谱反射率显著上升(P0.05),3种草坪草抗旱性能的强弱顺序为:高羊茅多年生黑麦草草地早熟禾。3种草坪草敏感波段光谱反射率(R_(550 nm),R_(760 nm))及高光谱参数(Rg,R0,GNDVI)与生理指标间呈显著或极显著相关,以R_(550 nm)与生理指标间的相关系数绝对值最大。同时R_(550 nm)构建了供试草坪草生理指标的估测模型,经检验,模型估测值与实测值相关性达显著或极显著水平。  相似文献   

A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response, and an immunostimulatory peptide   总被引：2，自引：0，他引：2  

Wang JL  Liu MQ  Han J  Chen WZ  Cong W  Cheng G  Gao YH  Lu YG  Chen JL  Zuo XP  Yan WY  Zheng ZX 《Veterinary microbiology》2007,125(3-4):224-231

The epitopes of the capsid of foot-and-mouth disease virus (FMDV) play important roles in the construction of highly immunogenic subunit vaccines. However few epitopes have been found for FMDV serotype Asia1. In this study we screened for epitopes of the VP1 and VP2 proteins of FMDV serotype Asia1 isolate, YNBS/58. Fragments consisting of amino acids 133-163 of VP1 and amino acids 1-33 of VP2 contained epitopes, and both induced lymphoproliferation in guinea pigs. Only the VP1 fragment induced neutralizing antibodies but the VP2 peptide dramatically increased the neutralizing antibody response induced by the VP1 peptide.  相似文献   

THE STUDY OF CYCLIC SOFTENING AND HARDENING BEHAVIORS IN A LOW ALLOY STEEL CONTAINING VANADIUM     

Sheng Guangmin Gong Sihong 《保鲜与加工》1988,(5)

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A Study of the Vertical Vibration of the 4200 Thick Plate Rolling Mills     

Zhu Caicao Wang Hongen Gong JianxiaoYu Shixing 《保鲜与加工》1993,(6):51-57

In this paper,the mill stand and the rolled piece were studied as a system. Based on the disoreted masses and the deformation of bending curve of the roller and roll housing, according to Rayleigh method,the equivalent mass and and the equivalent stiffness of the mode were solved,and set up a multiple degree of freedom vibration dynamic model. And also did theoretical analysis and calculation with an exapmle of the 4200 thick plate rolling mills.  相似文献   

THE STUDY OF STRAIN FATIGUE CRACK PROPAGATION BEHAVOURS OF VANADIUM BEARING HSLA STEEL     

Sheng Guangmin Gong Shihong Zhu Jinfu 《保鲜与加工》1989,(2):67-72

In this paper, the strain fatigue: crack propagation rates of vanadium bearing HSLA steel with different micro-structures are measured under constant load conditions, and the relationship between the crack propagation rate and the microstructures of steel is also studied.  相似文献   

A Study on the Mathematical Models for Two Dimensional Guillotine Cullting-stock     

Cai Zhengjun  Gong Jian  Liu Fei 《保鲜与加工》1996,(2):82-88

Based on the analysis and study on some existed models for two-dimensional Guillotine Cutting-Stock problem, it is pointed out that some kinds of the models may miss some good cutting patterns,and some may result in inadequate solutions because of only considering the patterns with the smallest waste. A kind of revised two-dimensional cutting-stock models are developed,which transform the two-dimensional problem into the one-dimensional one using the dimension-decreasing heuristic method and whose initial patterns are considered comprehensively. In this kind of models, not only the least waste,but also the other constraints,such as the demands and the relative area of the order plates are considered.  相似文献   

A Study on the Practical Algorithm for One Dimensional Equal Stock Cutting Problem     

Gong Jian Liu Fei Xu Zhongjun 《保鲜与加工》1997,(1):92-97

Based on alalysing the mathematical models for one dimensional equal stock cutting problem,a knapsack column generation algorithm has been constructed,which is a successive approximate algorithm for the practical engineering.In the end of this paper,some computational examples have been presented,which show that the convergence and optimum results of the algorithm are quite good.  相似文献