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81.
In dogs, secondary polycythemia (SP) may be associated with polyuria and polydipsia (PU/PD). The pathogenesis of this PU/PD has not yet been explained. We hypothesized that hyperviscosity and increased blood volume in SP might affect vasopressin (VP) release, resulting in PU/PD. This hypothesis was tested in 2 dogs with SP caused by renal neo-plasia and PU/PD. Osmoregulation of VP release was studied by a modified water deprivation test and by investigating the VP response to hypertonic saline infusion.
Water deprivation test results were consistent with an inability to produce concentrated urine despite increasing plasma osmolality. During hypertonic saline infusion, the osmotic threshold of VP release was markedly increased in both dogs, resulting in a delayed VP response to increasing plasma osmolality. The sensitivity of VP release was low normal in both dogs. We conclude that blood hyperviscosity and increased blood volume led to impaired VP release and polyuria.  相似文献   
82.
83.
Summary Visual assessment, black & white image analysis and colour image analysis of the severity of powdery mildew (Sphaerotheca fuliginea) infection on leaves of cucumber (Cucumis sativus) were compared. The extent of infection with powdery mildew was expressed as the number of spores per cm2. Statistical treatment of the data showed colour image analysis to be superior to the other two methods of evaluation. Major veins, light reflections and variations in the colour of leaves can degrade the accuracy of colour image analysis somewhat. These problems are, however, surmountable. Colour image analysis qualifies as a valuable new tool for accurate, objective, reproducible and detailed quantitative assessment of disease on leaves of plants.Abbreviations H hue - I intensity - IA image analysis - PM powdery mildew - S saturation  相似文献   
84.
Summary Progeny lines of chromosome-doubled haploids from crosses between one winter and four spring barley varieties were analysed for winter hardiness and vernalization requirement in the field. About one quarter or less of the offspring lines required vernalization under field conditions showing that winter versus spring habit of growth is controlled by at least two pairs of genes. The vernalization requirement could be measured quantitatively by testing a few plants in a greenhouse after artificial cold treatment during germination. The individual winter lines tested required from two to seven weeks of vernalization in order to flower as early as possible. The cold treatment had no effect on the time of flowering in the spring lines.Winter hardiness varied from zero to 100% plant survival in the field. The majority of the lines requiring vernalization survived better than 90% and the survival of the rest was at least 60%. The winter survival of the spring lines varied from zero per cent (about one third of the lines) to 100%. Hence, it is possible to obtain lines of good winter hardiness without requirements for cold treatment. Conversely, a selection for vernalization requirement has a positive effect on winter hardiness.  相似文献   
85.
This paper examines the roles of physical capital, human capital, and social capital in China's economic growth during the reform period 1981–2010. Empirical estimation confirms that physical capital and human capital contribute to the economic growth, probably due to the capital accumulation and the improvement of labor productivity. The impact of social capital turns from being insignificant in the 1980s and the 1990s to be positive in the 2000s, suggesting its rising importance in recent decades. A declining role of physical capital in the economic growth in China from 1990s to 2000s is also found. The findings hold for several additional robustness checks, including focusing on longer term determinants of the economic growth, subregional analysis, and endogeneity. Furthermore, the foreign direct investment inflows and adjustment of economic ownership structure are also important for economic growth in China.  相似文献   
86.
Protoplast fusion can be used to produce somatic hybrids of species that cannot be obtained by sexual hybridization. The possibility to introgress genes from Solanum species into the cultivated tomato species Lycopersicon esculentum, and to obtain novel cytoplasm-nucleus combinations (cybrids) was considered as an important strategy to extend the genetic variation available for tomato breeding. Somatic hybrids between L. esculentum and other Lycopersicon species, as well as between L. esculentum and Solanum or Nicotiana species, have been produced. Specific mutants, genotypes with antibiotic resistances, and metabolic inhibition by iodoacetate or iodoacetamide and irradiation were used for the selection of hybrids. In addition, the improvement of protoplast culture techniques and the use of the favourable tissue culture traits derived from species such as L. peruvianum, which have been introduced into tomato by classical breeding, allowed the efficient recovery of somatic hybrids. However, the occurrence of somatic incongruity in fusion combinations of L. esculentum and Solanum and even more in L. esculentum and Nicotiana, did not allow the production of true cybrids and/or fertile hybrids, indicating the importance of both cytoplasm-nucleus and nucleus-nucleus interactions in somatic incongruity. Another problem with fusions between distantly related species is the strongly reduced fertility of the hybrids and the very limited homoeologous recombination between chromosomes of the parental species. Partial genome transfer from donor to recipient through microprotoplast (+) protoplast fusion, and the production of monosomic or disomic chromosome addition lines, light overcome some of these problems. In symmetric somatic hybrids between L. esculentum and S. tuberosum the occurrence of limited somatic and meiotic recombination was demonstrated. Fertile progeny plants could be obtained, though at a low frequency, when embryo rescue was performed on a large scale after backcrossing hexaploid somatic tomato (+) potato hybrids with a tetraploid potato genotype. The potential value of genomic in situ hybridization (GISH) and RFLPs for the analysis of the genome/chromosome composition of the hybrids has been demonstrated for intergeneric somatic hybrids between Lycopersicon and Solanum.Abbreviations cpDNA chloroplast DNA - mtDNA mitochondrial DNA  相似文献   
87.
Cassava roots, a major food in Africa, contain cyanogenic glucosides that may cause toxic effects. Malawian women farmers considered fields of seemingly similar cassava plants to be mixes of both ‘cool’ and ‘bitter’ cultivars. They regard roots from ‘cool’ cultivars as non-toxic. Roots of ‘bitter’ were considered to require extensive traditional processing done by women to be safe for consumption. But curiously, these women farmers preferred ‘bitter’ cultivars since toxicity confers protection against theft, which was a serious threat to the food security of their families. We studied how well these farmers comprehend the effects of genetic variations in cassava when dealing with cyanogenesis in this complex system. Using molecular markers we show that most plants farmers identified as belonging to a particular named cultivar had a genotype typical of that cultivar. Farmers' ethno-classification into ‘cool’ and ‘bitter’ cultivars corresponded to a genetic sub-division of the typical genotypes of the most common cultivars, with four-fold higher cyanogenic glucoside levels in the bitter cultivars. Examining morphology, farmers distinguished genotypes better than did the investigators when using a standard botanical key. Undoubtedly, these women farmers grasp sufficiently the genetic diversity of cassava with regard to cyanogenesis to simultaneously benefit from it and avoid its dangers. Consequently, acyanogenic cassava – the breeding of which is an announced good of some cassava genetic improvement programmes – is not a priority to these farmers. Advances in molecular genetics can help improve food supply in Africa by rapid micropropagation, marker assisted breeding and introduction of transgenic varieties, but can also help to elucidate tropical small-scale farmers' needs and skills. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   
88.
89.
Acidification of surface waters and forest soils is severe in large parts of southern Sweden. The shallow groundwaters are also affected. Large scale liming of surface waters and streams is in operation, often combined with wetland liming to limit the effects of acid episodes, e.g. at snow melt. Acid episodes are perhaps the most severe problem in limed surface waters and in many as yet well buffered waters, because of temperature-layered acid inflow, often superficial. As a result of some investigations, a large scale forest liming programme covering 6.500–10.000 km2 was recently suggested. The main objectives of this forest liming programme are to retard cation depletion and to prevent nutrient imbalance and forest decline in acidified areas. This paper deals with the effects of forest soil liming on streams and surface waters. The response of water chemistry is very dependent on hydrological and soil properties. Although pH itself may be little affected by liming, the acidity (or negative ANC) decreases, inorganic Al-species decrease and the Al/BC-ratio increases in the runoff water. Especially interesting is that this is also true during acid episodes. This means that toxicity for acid sensitive biota decreases. These results indicate that large scale liming may have beneficial effects on surface water chemistry. Furthermore, as surface waters are expected to respond to smaller decreases in acid deposition than do forests soils, forest soil liming may allow less frequent liming of lakes. Consequently, forest soil liming in combination with the anticipated emission reductions may have very beneficial results on surface waters in certain areas of Sweden.  相似文献   
90.
A 90‐day laboratory incubation study was carried out using six contrasting subtropical soils (calcareous, peat, saline, noncalcareous, terrace, and acid sulfate) from Bangladesh. A control treatment without nitrogen (N) application was compared with treatments where urea, ammonium sulfate (AS), and ammonium nitrate (AN) were applied at a rate of 100 mg N (kg soil)–1. To study the effect of N fertilizers on soil carbon (C) turnover, the CO2‐C flux was determined at nine sampling dates during the incubation, and the total loss of soil carbon (TC) was calculated. Nitrogen turnover was characterized by measuring net nitrogen mineralization (NNM) and net nitrification (NN). Simple and stepwise multiple regressions were calculated between CO2‐C flux, TC, NNM, and NN on the one hand and selected soil properties (organic C, total N, C : N ratio, CEC, pH, clay and sand content) on the other hand. In general, CO2‐C fluxes were clearly higher during the first 2 weeks of the incubation compared to the later phases. Soils with high pH and/or indigenous C displayed the highest CO2‐C flux. However, soils having low C levels (i.e., calcareous and terrace soils) displayed a large relative TC loss (up to 22.3%) and the added N–induced TC loss from these soils reached a maximum of 10.6%. Loss of TC differed depending on the N treatments (urea > AS > AN >> control). Significantly higher NNM was found in the acidic soils (terrace and acid sulfate). On average, NNM after urea application was higher than for AS and AN (80.3 vs. 71.9 and 70.9 N (kg soil)–1, respectively). However, specific interactions between N‐fertilizer form and soil type have to be taken into consideration. High pH soils displayed larger NN (75.9–98.1 mg N (kg soil)–1) than low pH soils. Averaged over the six soils, NN after application of urea and AS (83.3 and 82.2 mg N (kg soil)–1, respectively) was significantly higher than after application of AN (60.6 mg N (kg soil)–1). Significant relationships were found between total CO2 flux and certain soil properties (organic C, total N, CEC, clay and sand content). The most important soil property for NNM as well as NN was soil pH, showing a correlation coefficient of –0.33** and 0.45***, respectively. The results indicate that application of urea to acidic soils and AS to high‐pH soils could be an effective measure to improve the availability of added N for crop uptake.  相似文献   
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