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241.
L.?BrillanteEmail authorView authors OrcID profile B.?Bois O.?Mathieu J.?Lévêque 《Precision Agriculture》2016,17(6):637-658
Electrical resistivity (ER) can be used to assess soil water in the field. This study investigated the possibility of extending the use of ER to measure plant available soil water variables, i.e. available soil water (ASW), total transpirable SW (TTSW), and fraction of transpirable SW (FTSW) using a pedotransfer approach. In a vineyard, 224 electrical resistivity tomography (ERT) transects and 672 time domain reflectometry (TDR) soil water profiles were acquired over 2 years. Soil physical–chemical properties were measured on 73 soil samples from eight different sites. To estimate the amount of soil water available to plants, grapevine (Vitis vinifera L.) water status was monitored by means of leaf water potentials. A benchmark experiment was carried out to compare four machine-learning techniques: multivariate adaptive regression splines (MARS), k-nearest neighbours (KNN), random forest (RF), and gradient boosting machine (GBM). Model interpretation led to a deeper understanding of the relationships between electrical resistivity and soil properties when predicting soil water availability for the plant. The models assessed had good predictive performance and were therefore used to map ASW, TTSW and FTSW in the vineyard. ER coupled to machine-learning algorithms was shown to be a good proxy for quantification and visualisation of plant available soil water with low disturbance. 相似文献
242.
Pasquet V Morisset P Ihammouine S Chepied A Aumailley L Berard JB Serive B Kaas R Lanneluc I Thiery V Lafferriere M Piot JM Patrice T Cadoret JP Picot L 《Marine drugs》2011,9(5):819-831
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL−1 (0.17 μM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL−1 (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL−1 (66.7 μM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained. 相似文献
243.
Mathieu Diribarne Anne Vaiman Michel Péchayre E. Pailhoux Xavier Mata Gérard Guérin Stéphane Chaffaux 《Journal of Equine Veterinary Science》2009
Association of seven candidate genes with cryptorchidism was investigated in the Thoroughbred. A pedigree composed of 23 cryptorchids and 24 nonaffected horses, sharing a common ancestor, was constituted. Sixteen microsatellite markers were developed either from bacterial artificial chromosomes (BAC) isolated for each candidate gene or by in silico screening. DNA from our pedigree was genotyped for these microsatellites. Statistical analysis of the allelic and genotypic frequencies observed with these markers did not reveal any association between the candidate genes and the cryptorchidism phenotype in our horse panel. 相似文献
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