Bovine tuberculosis in South Darfur State, Sudan: an abattoir study based on microscopy and molecular detection methods     

El Tigani A. Asil  Sulieman M. El Sanousi  Ahmed Gameel  Haytham El Beir  Maha Fathelrahman  Nasir M. Terab  Magzoub A. Muaz  Mohamed E. Hamid 《Tropical animal health and production》2013,45(2):469-472

Bovine tuberculosis (BTB) is a widespread zoonosis in developing countries but has received little attention in many sub-Saharan African countries including Sudan and particularly in some parts such as Darfur states. This study aimed to detect bovine tuberculosis among caseous materials of cattle slaughtered in abattoirs in South Darfur State, Sudan by using microscopic and PCR-based methods. The study was a cross-sectional abattoir-based study which examined a total of 6,680 bovine carcasses for caseous lesions in South Darfur State between 2007 and 2009. Collected specimens were examined for the presence of acid-fast bacilli (AFB) by using microscopic and culture techniques. Isolated mycobacteria were identified by selected conventional cultural and biochemical tests in comparison to a single tube multiplex PCR (m-PCR) assay which detect Mycobacterium bovis-specific 168-bp amplicons. Of the total 6,680 slaughtered cattle examined in South Darfur, 400 (6 %) showed caseations restricted to lymph nodes (86.8 %) or generalized (13.2 %). Bovine tuberculosis was diagnosed in 12 (0.18 %), bovine farcy in 59 (0.88 %), unidentified mycobacteria in 6 (0.09 %), and missed or contaminated cultures in 7 (0.1 %). Out of 18 cultures with nonbranching acid-fast rods, 12 amplified unique 168-bp sequence specific for M. bovis and subsequently confirmed as M. bovis. With the exception of the reference M. tuberculosis strains, none of the remaining AFB amplified the 337-bp amplicon specific for M. tuberculosis. It could be concluded that bovine tuberculosis is prevalent among cattle in South Darfur representing 4.5 % from all slaughtered cattle with caseous lesions. The study sustains microscopy as a useful and accessible technique for detecting AFB. m-PCR assay proved to be valuable for confirmation of BTB and its differentiation from other related mycobacteriosis, notably bovine farcy.  相似文献   

Potassium fixation of some calcareous soils after short term extraction with different solutions     

Mahdi Najafi-Ghiri  Soodabeh Rezabigi  Sara Hosseini  Hamid Reza Boostani  Hamid Reza Owliaie 《Archives of Agronomy and Soil Science》2019,65(7):897-910

This investigation was done to determine the release of potassium (K) from five calcareous soils of southern Iran using 0.025 M CaCl₂, HCl and citric acid during six successive extractions and to study the K fixation capacity of the soils after K release experiment. Mineralogical study indicated that Vertisols and Mollisols were dominated with smectites; while other soils had illite, chlorite, palygorskite and smectite. Results indicated that citric acid extracted more K than CaCl₂ and HCl (137 vs. 111 and 113 mg kg^?1, respectively). The analysis of calcium (Ca), magnesium (Mg) and K concentrations in the solutions suggests that the exchange of K with soluble Ca and Mg (originated from dissolution of carbonates by acidic solutions) is the main mechanism of K release, but citrate is able to dissolve K-bearing minerals and release K in slightly calcareous soils. Soils with more illite released more K. Potassium fixation capacity of soils increased after extractions of soils with different extractants from 324 to 471 mg kg^?1, with no significant difference. It is suggested to apply more K fertilizers in K-depleted calcareous soils and use of different solutions for extracting K from soil minerals may be a temporary and short term solution.  相似文献   

Reduction in fusarium toxin levels in corn silage with low dry matter and storage time     

Boudra H  Morgavi DP 《Journal of agricultural and food chemistry》2008,56(12):4523-4528

Under unfavorable climatic conditions, Fusarium spp. can contaminate corn plants in the field and produce toxins that are present at the time of ensiling. The stability of deoxynivalenol, fumonisins B1 and B2, and zearalenone in corn silage was tested over two consecutive years. Variables studied were corn dry matter (DM) and storage length and temperature. The concentration of all Fusarium toxins decreased upon ensiling ( P < 0.001). Increasing the length of storage and ensiling with low DM resulted in a higher rate of toxin disappearance, particularly for the water soluble toxins deoxynivalenol and fumonisin B1. Toxin disappearance ranged from 50% for zearalenone to 100% for deoxynivalenol. In contrast, temperature did not have any effect on stability ( P > 0.05). These results indicate that low DM at ensiling as well as a prolonged storage could be a practical way to reduce or eliminate some Fusarium toxins in contaminated silages.  相似文献   

Research on the Relationship of Water Resource and Vegetation Distribution in Tarim Populus euphraticas Nature Reserve     

XI Xiu-mei  DUAN Shu-guo  Hamid YIMIT 《水土保持研究》2008,15(2)

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Shortening the generation cycle in faba bean (Vicia faba) by application of cytokinin and cold stress to assist speed breeding     

Saeid Mobini  Hamid Khazaei  Thomas D. Warkentin  Albert Vandenberg 《Plant Breeding》2020,139(6):1181-1189

The aim of this study was to reduce the length of the breeding cycle for faba bean by accelerating seed setting. We examined the mode and time of exogenous 6-benzylaminopurine (BAP) (cytokinin) application, and cold treatments and their combinations in two faba bean genotypes. Acropetal node number of pod and seed set and pollen viability were recorded during the experiments. Application of BAP improved pollen germination. The application of 10^–5 M BAP 4 days after flowering increased seed set at the lower nodes. Cold treatment (8/4°C day/night for 2 days) after the onset of flowering induced the formation of more pods and faster pod set compared to the non-cold treatment. The time to first seed was significantly reduced, and pollen viability was increased in plants exposed to cold treatment. Increased pollen viability also showed a significant positive correlation with seed setting. The combinations of 10^–5 BAP and cold treatment together had similar and independent effects. These results will accelerate plant breeding in faba bean by providing additional tools for reducing generation time.  相似文献   

Bee pollen and propolis as dietary supplements for rabbit: Effect on reproductive performance of does and on immunological response of does and their offspring     

Youssef A. Attia  Fulvia Bovera  Abd El‐Hamid Abd Elhamid  Sameer A. Nagadi  Mohamed A. Mandour  Saber Sh. Hassan 《Journal of animal physiology and animal nutrition》2019,103(3):959-968

To evaluate the effect of bee pollen (BP) and/or propolis (Pro) supplementation on rabbit does, 64 nulliparous NZW rabbits does were distributed among eight groups (eight animals/group). One unsupplemented group was the control; the other seven groups were supplemented, respectively, with zinc bacitracin (ZnB) at 100 mg, BP at 150 and 300 mg, Pro at 150 and 300 mg, BP+Pro at 150 and 300 mg of each three times/week, day after day continuously along eight parities. The BP300, Pro300 and BP+Pro150 groups had higher body weight of litter at birth and number of kids born alive. The BP supplementation at 150 mg increased plasma total protein and albumin than the control group. The BP or Pro at 150 mg decreased plasma T₃ than the other groups except for BP+Pro150. The ZnB group had significantly greater T₃/T₄ ratio compared to BP, Pro and BP+Pro at 150 mg. The BP+Pro150 group had less ALT than the control; BP300 and Pro 300 mg resulted in lower plasma AST than the groups Pro150 with or without BP and the control group. The plasma alkaline phosphatase of BP at 150 or 300 mg and BP+Pro150 was significantly greater than that of the Pro150 group. The BP+Pro300 group had higher WBCs than the other groups. In contrast, the lymphocytes were greater in the Pro and BP+Pro300 groups than in BP, Pro and BP+Pro at 150 mg. The groups supplemented with BP and BP+Pro at 150 and 300 mg had significantly greater SRBCs of doe rabbits and their offspring compared to the control and the ZnB group. The BP at 300 mg increased the serum albumin and α₁‐globulin than the control group. The Pro300 group had greater serum α₂‐globulin and β‐globulin than the control group. The total globulin was significantly greater for the 300 mg propolis‐supplemented groups than the control.  相似文献   

Which source and level of dietary sodium is appropriate for broiler chickens reared in a high‐altitude area?     

Hamid Reza Taheri  Hadi Nasiri  Rasoul Ahmadkhani 《Journal of animal physiology and animal nutrition》2019,103(4):1090-1098

This study investigated effect of increasing level of dietary sodium using sodium bicarbonate or sodium chloride on growth performance, mortality, characteristics of carcass, organs and tibia, calcium and phosphorus of serum in broilers reared in a high‐altitude area (1,700 m above sea level). A total of 588 Ross 308 male broiler chicks were used in seven treatments, six replicates per treatment of 14 birds per each from 1 to 38 d of age. Seven dietary treatments consisted of a basal diet (with 0.16% sodium and 0.23% chloride), top‐dressed for six diets to give three supplementary levels of sodium (0.07%, 0.14% and 0.21%) from sodium bicarbonate (respectively by 0.26%, 0.52% and 0.78%) or sodium chloride (respectively by 0.18%, 0.36% and 0.54%), resulting in seven diets with total sodium and chloride levels of 0.16% and 0.23%, 0.23% and 0.23%, 0.30% and 0.23%, 0.37% and 0.23%, 0.23% and 0.33%, 0.30% and 0.44%, 0.37% and 0.55% respectively. Increasing sodium level improved feed conversion ratio (FCR) linearly and quadratically. However, when FCR was calculated without adjusting for feed intake of mortalities, the enhanced sodium level did not improve this parameter. Increasing sodium level via sodium chloride enhanced ascites mortality, total mortality, relative weight of heart and right ventricle linearly. Increasing sodium level reduced serum calcium and enhanced serum phosphorus linearly; however, there was a linear tendency to increase tibia ash when sodium level was enhanced by sodium bicarbonate (p = 0.08) or sodium chloride (p = 0.07). Increasing sodium level via sodium bicarbonate tended (p = 0.08) to reduce tibia strength linearly. In conclusion, a diet with 0.16% sodium and 0.23% chloride is enough for broiler chicken reared in a high‐altitude area, and increasing dietary sodium level via sodium chloride has detrimental effect on survivability of broiler in such condition.  相似文献   

Low developmental competence and high tolerance to thermal stress of ovine oocytes in the warm compared with the cold season     

Ahmadi  Ebrahim  Nazari  Hassan  Hossini-Fahraji  Hamid 《Tropical animal health and production》2019,51(6):1611-1618

Tropical Animal Health and Production - Heat stress can potentially affect most aspects of reproduction in mammals. To our knowledge, no studies have ever been conducted for evaluating the...  相似文献   

Comparative evaluation of different therapeutic protocols for contagious caprine pleuropneumonia in Himalayan Pashmina goats     

Yatoo  Mohd. Iqbal  Parray  Oveas Raffiq  Mir  Muheet  Bhat  Riyaz Ahmed  Malik  Hamid Ullah  Fazili  Mujeeb ur Rehman  Qureshi  Sabia  Mir  Masood Salim  Yousuf  Raja Wasim  Tufani  Noor Alam  Dhama  Kuldeep  Bashir  Shah Tauseef 《Tropical animal health and production》2019,51(8):2127-2137

Tropical Animal Health and Production - Therapeutic management of contagious caprine pleuroneumonia (CCPP) involves mostly the use of oxytetracycline followed by enrofloxacin and rarely tylosin. In...  相似文献   

Phytomass estimation using eMODIS NDVI and ground data in arid rangelands of Morocco     

Hamid Mahyou  Bernard Tychon  Marie Lang  Riad Balaghi 《African Journal of Range and Forage Science》2018,35(1):1-12

The assessment of rangeland productivity in semi-extensively grazed arid rangelands is a prerequisite for livestock management in relation to sustainable use of pastoral resources. The objective of this study was to assess rangeland productivity based on normalised difference vegetation index (NDVI) images. Data on phytomass were measured on 61 field samples in arid rangelands of Morocco, covering various rangeland categories during autumn (November) and spring (April), i.e. when phytomass is at low and high levels, respectively, for two consecutive years (2008 and 2009). Dekadal EROS Moderate Resolution Imaging Spectroradiometer (eMODIS) NDVI data were linearly regressed to field measurements for these four periods. Results show that phytomass values were correlated with NDVI during spring, with R² and RMSE values of 0.82 and 0.3 t ha^?1, respectively. This study indicates there is a high potential for operational use of remotely sensed data to estimate rangeland phytomass of semi-extensively grazed rangelands.  相似文献