Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue.     

S D Sorden  P A Harms  P Nawagitgul  D Cavanaugh  P S Paul 《Journal of veterinary diagnostic investigation》1999,11(6):528-530

An immunohistochemical method for the detection of type 2 porcine circovirus (PCV2) in paraffin-embedded tissue was developed. Rabbits were inoculated with purified PCV2 to obtain a polyclonal antiserum. Antiserum was applied to sections of porcine tissue that contained lesions consistent with postweaning multisystemic wasting syndrome and in which PCV2 genome had been demonstrated by in situ hybridization. In all cases (18/18), the density and distribution of positive cells detected by in situ hybridization or immunohistochemistry were identical. The immunohistochemical method is more rapid and less expensive than in situ hybridization and is thus more suitable for routine diagnostic use.  相似文献   

Technique of mandibular salivary gland biopsy in river otters (Lutra canadensis).     

M E Tocidlowski  C A Harms  P W Sumner  M K Stoskopf 《Journal of zoo and wildlife medicine》1999,30(2):252-255

A Franklin-Silverman biopsy needle was used to obtain 2-5- x 1-2-mm mandibular salivary gland tissue samples percutaneously from nine North American river otters (Lutra canadensis). The samples were suitable for fluorescent antibody or polymerase chain reaction rabies testing. Ninety-two percent (11/12) of the biopsy procedures yielded histologically confirmed salivary gland tissue, and the remaining biopsy yielded adipose tissue. No complications were noted after 5-21 days.  相似文献   

Epipolythiodiketopiperazines from the Marine Derived Fungus Dichotomomyces cejpii with NF-κB Inhibitory Potential     

Henrik Harms  Barbora Orlikova  Seungwon Ji  Damun Nesaei-Mosaferan  Gabriele M. K?nig  Marc Diederich 《Marine drugs》2015,13(8):4949-4966

The Ascomycota Dichotomomyces cejpii was isolated from the marine sponge Callyspongia cf. C. flammea. A new gliotoxin derivative, 6-acetylmonodethiogliotoxin (1) was obtained from fungal extracts. Compounds 2 and 3, methylthio-gliotoxin derivatives were formerly only known as semi-synthetic compounds and are here described as natural products. Additionally the polyketide heveadride (4) was isolated. Compounds 1, 2 and 4 dose-dependently down-regulated TNFα-induced NF-κB activity in human chronic myeloid leukemia cells with IC_50s of 38.5 ± 1.2 µM, 65.7 ± 2.0 µM and 82.7 ± 11.3 µM, respectively. The molecular mechanism was studied with the most potent compound 1 and results indicate downstream inhibitory effects targeting binding of NF-κB to DNA. Compound 1 thus demonstrates potential of epimonothiodiketopiperazine-derived compounds for the development of NF-κB inhibitors.  相似文献   

产蛋鸡色氨酸的需要量     

李海艳  王志祥  夏素银 《饲料研究》2010,(9)

选用36周龄的海兰蛋鸡用于试验,对照组日粮含0.2%的色氨酸,试验日粮色氨酸水平分别为0.12%、0.13%、0.14%、0.15%、0.16%、0.17%和0.18%,共8组。对所有日粮补充氨基酸以确保色氨酸是第一限制性氨基酸,有研究表明:通过补充蛋氨酸可控制饮食(0.2%色氨酸)。在基础日粮中添加色氨酸可显著提高产蛋率(EP)、蛋质量(EW)和蛋内容物(EC)。折线回归表明:当每天每只蛋鸡EC为45.4g时,EP和EC对色氨酸的需要量分别为139.8和149mg。  相似文献   

Effect of Early Postpartum Ovulation on Fertility in Dairy Cows     

KN Galvo  M Frajblat  WR Butler  SB Brittin  CL Guard  RO Gilbert 《Reproduction in domestic animals》2010,45(5):e207-e211

Our objectives were to determine the effects of early ovulation on fertility and uterine health of dairy cows. Four hundred and forty‐five Holstein cows (185 primiparous and 260 multiparous) from five herds were used. Blood samples were collected at 21, 35 and 49 days in milk (DIM) and cows were considered to be cyclic at 21 DIM (Cyc21) if serum progesterone (P4) concentration was above 1 ng/ml, cyclic by 49 DIM (Cyc49) if P4 concentration was above 1 ng/ml at 35 or 49 DIM, or not cyclic (NotCyc) if P4 concentration was below 1 ng/ml at all sample times. Endometrial cytology for diagnosis of subclinical endometritis was examined at 49 DIM in a subset of 414 cows. Cows in the group Cyc21 had increased hazard of insemination, for the first service, compared with cows in Cyc49 [hazard ratio (HR) = 1.40; 95% CI = 1.10–1.79; p = 0.006] and NotCyc (HR = 2.07; 95% CI = 1.52–2.82; p < 0.001). Cows in the Cyc49 group also had increased hazard of insemination compared with cows in the NotCyc group (HR = 1.47; 95% CI = 1.13–1.93; p = 0.005). Median days to insemination were, respectively, 71, 76 and 96 for cows in Cyc21, Cyc49 and NotCyc groups. Cows in Cyc21 had greater first service pregnancy per AI than Cyc49 [38.6 vs 28.1%; adjusted odds ratio (AOR) = 1.67; 95% CI = 1.01–2.75; p = 0.04] and NotCyc (38.6 vs 23.6%; AOR = 2.08; 95% CI = 1.08–4.00; p = 0.03). Pregnancy per AI was similar in Cyc49 and NotCyc cows (28.1 vs 23.6%; AOR = 1.25; 95% CI = 0.70–2.24; p = 0.45). Cows in Cyc21 had increased hazard of pregnancy up to 300 DIM compared with Cyc49 (HR = 1.52; 95% CI = 1.17–1.96; p = 0.002) and NotCyc (HR = 1.98; 95% CI = 1.41–2.78; p < 0.001). Cows in Cyc49 tended to have increased hazard of pregnancy compared with NotCyc (HR = 1.31; 95% CI = 0.96–1.77; p = 0.09). Median days to pregnancy were, respectively, 103, 147 and 173 for cows in Cyc21, Cyc49 and NotCyc groups. Cows in the Cyc21 group had decreased prevalence of subclinical endometritis compared with cows in the NotCyc group (29.9 vs 43.7%; AOR = 0.53; 95% CI = 0.29–0.97; p = 0.04); however, the prevalence did not differ from the Cyc49 group (29.9 vs 39.1%; AOR = 0.68; 95% CI = 0.41–1.14; p = 0.15). Cyc49 cows had similar prevalence of subclinical endometritis compared with NotCyc cows (AOR = 0.77; 95% CI = 0.46–1.29; p = 0.32). Early postpartum ovulation was associated with improved uterine health and fertility.  相似文献   

Biochemical factors in wheat (Triticum aestivum L.) involved in the tetany syndrome     

C. L. Harms  L. S. Murphy 《Journal of plant nutrition》2013,36(7):1027-1041

Nitrogen in winter wheat (Triticum aestivum L.) forage was fractionated into water‐soluble protein N (WSPN) and non‐protein N (NPN). Both WSPN and NPN can be rapidly converted to ammonia in the rumen which may increase pH of rumen fluid and decrease solubility of Mg compounds and lead to increased incidence of grass tetany. In plant samples taken after spring growth initiation, WSPN represented approximately 50% of total N and NPN represented approximately 25% of total N. Total N concentration and relative percentages of WSPN and NPN decreased with advancing plant maturity in 1974, but during 1975 the relative WSPN and NPN percentages remained relatively constant at 43 to 55% and 18 to 30% of total N, respectively. Total N, WSPN, and NPN concentrations decreased as plants matured.

Total water‐soluble carbohydrates (TWSC) were determined and N/TWSC ratios were calculated in wheat forage. TWSC concentrations increased from the initial sampling date to a peak concentration and then declined during the latter part of the sampling period. N/TWSC ratios were widest at the initial sampling date and decreased markedly in subsequent samplings.

Organic acid concentrations were determined in 1975 wheat forage samples by gas‐liquid chromatography. Citric and trans‐aconitic acids have been implicated in the etiology of grass tetany as chelating agents for Mg and Ca. Chelation in the rumen fluid may reduce Mg and Ca absorption or chelation in the blood serum may reduce biological activity. Malic and aconitic acids accumulated in wheat. Malic acid concentration increased with advancing plant maturity to a maximum of 1.70% (dry weight basis). Aconitic acid concentrations were highest at the mid‐point in the sampling period, a maximum of 1.24% being determined.  相似文献   

Utilization of a rate enhancement hybridization buffer system for rapid in situ hybridization for the detection of porcine circovirus in cell culture and in tissues of pigs with postweaning multisystemic wasting syndrome.   总被引：7，自引：0，他引：7  

T Sirinarumitr  I Morozov  P Nawagitgul  S D Sorden  P A Harms  P S Paul 《Journal of veterinary diagnostic investigation》2000,12(6):562-565

A rapid in situ hybridization (ISH) technique for the detection of porcine circovirus (PCV) nucleic acid in cell culture and formalin-fixed paraffin-embedded tissues was developed. A fluorescein-labeled RNA probe was transcribed from a plasmid containing 530 bp of the ORF1 of a PCV isolated from a pig with postweaning multisystemic wasting syndrome (PMWS). Hybridization using standard hybridization buffer was performed at 42 C for 16 hours and was compared to hybridization using rate enhancement hybridization (REH) buffer at 67 C for 2 hours. Hybridization was detected with an alkaline phosphatase-conjugated antifluorescein antibody. In both cultured cells and tissues from pigs with PMWS, the signal intensity and number of labeled cells in sections hybridized with REH buffer were equal to those of sections hybridized with standard hybridization buffer. The total time required for ISH using the REH buffer is 7-8 hours, thus making this protocol suitable for application in routine PCV diagnosis.  相似文献   

Dilated (congestive) cardiomyopathy in a Friesian heifer   总被引：1，自引：0，他引：1  

MW McLENNAN  WR KELLY † 《Australian veterinary journal》1990,67(2):75-76

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Beta‐carotene supplementation of Laying Hen Diets     

B. L. Damron  S. R. Goodson  R. H. Harms  D. M. Janky  H. R. Wilson 《British poultry science》1984,25(3):349-352

1. The possibility of β‐carotene affecting production of hens or hatchability of eggs was studied over 252 d with diets based on white maize containing 0, 1·5, 6·75 or 15 mg added β‐carotene/kg.

2. Egg production, food intake, food utilisation, egg weight, and egg specific gravity were not affected significantly by jS‐carotene. Egg fertility, hatch‐ability and final body weights of hens were also not significantly influenced.

3. Reflectance measurements showed that dietary β‐carotene resulted in increased deposition and more intense pigmentation in the egg yolk.

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Eisenmenger complex in two calves     

MW McLENNAN  WR KELLY†  RH SUTTON† 《Australian veterinary journal》1996,74(1):22-25

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