Susceptibility of larvae and adults of Monochamus galloprovincialis to entomopathogenic fungi under controlled conditions     

Ricardo Petersen-Silva  Lurdes Inácio  Joana Henriques  Pedro Naves  Edmundo Sousa  Juli Pujade-Villar 《国际虫害防治杂志》2013,59(2):106-112

Six entomopathogenic fungal isolates were tested under controlled conditions, as biological control agents against Monochamus galloprovincialis (Coleoptera: Cerambycidae) (Olivier), the vector of the pine wood nematode Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae) (Steiner and Buhrer). The fungi Beauveria bassiana (Bals.-Criv.) Vuill, Metarhizium anisopliae (Metchnikoff) Sorokin, Metarhizium sp. and Fusarium sp. were tested against larvae and adults of the insect, by comparing two exposure techniques: continuous contact and spraying. Regarding the larvae, only Fusarium sp. and Metarhizium sp. killed more than 40% of the individuals, while B. bassiana and M. anisopliae were found to kill 50% of the adults (LT₅₀) within five days. Exposure to B. bassiana reduced adult longevity by 19 days, when compared to untreated beetles, and had a direct efficacy of 46%. Continuous contact with the fungi proved to be more effective than spraying. A larval disinfection protocol was developed to eliminate other fungal isolates from the wood-collected tested individuals. The implications of the results and possible applications of selected strains as bio-control agents against M. galloprovincialis are discussed.  相似文献   

Nitrite toxicity to Litopenaeus schmitti (Burkenroad, 1936, Crustacea) at different salinity levels          下载免费PDF全文

Edison Barbieri  Ana Cristina Vigliar Bondioli  Camila Batista de Melo  Marcelo Barbosa Henriques 《Aquaculture Research》2016,47(4):1260-1268

Litopenaeus schmitti juveniles (total length 15 ± 0.4 cm) were exposed to different concentrations of nitrite using the static renewal method at different salinity levels (5‰, 20‰ and 35‰) at pH 8.0 and 20 °C. The 24, 48, 72 and 96 h LC50 values of nitrite in L. schmitti juveniles were 40.72, 32.63, 24.63, and 19.12 mg L⁻¹ at 5‰; 53.52, 38.60, 27.76, 25.55 mg L⁻¹ at 20‰; 54.32, 47.87, 41.67 and 38.88 mg L⁻¹ at 35‰ salinity. As the salinity decreased from 35‰ to 5‰, susceptibility to nitrite increased by 33.4%, 46.7%, 69.2% and 103.3%, after 24, 48, 72 and 96 h of exposure respectively. Furthermore, we found that exposure of shrimp to nitrite caused an increase in oxygen consumption by 137.3%, 99.2% and 81.4% and an increase in the ammonia excretion level by 112.5%, 87% and 64.3% with respect to the control with decreasing salinity levels.  相似文献   

Conformity and controversies in the diagnosis,staging and follow‐up evaluation of canine nodal lymphoma: a systematic review of the last 15 years of published literature          下载免费PDF全文

L. Marconato  G. A. Polton  S. Sabattini  M. Dacasto  O. A. Garden  I. Grant  T. Hendrickx  J. Henriques  G. Lubas  E. Morello  D. Stefanello  S. Comazzi 《Veterinary and comparative oncology》2017,15(3):1029-1040

Diagnostic methods used in the initial and post‐treatment evaluation of canine lymphoma are heterogeneous and can vary within countries and institutions. Accurate reporting of clinical stage and response assessment is crucial in determining the treatment efficacy and predicting prognosis. This study comprises a systematic review of all available canine multicentric lymphoma studies published over 15 years. Data concerning diagnosis, clinical stage evaluation and response assessment procedures were extracted and compared. Sixty‐three studies met the eligibility criteria. Fifty‐five (87.3%) studies were non‐randomized prospective or retrospective studies. The survey results also expose variations in diagnostic criteria and treatment response assessment in canine multicentric lymphoma. Variations in staging procedures performed and recorded led to an unquantifiable heterogeneity among patients in and between studies, making it difficult to compare treatment efficacies. Awareness of this inconsistency of procedure and reporting may help in the design of future clinical trials.  相似文献   

Biogeographical region and environmental conditions drive functional traits of estuarine fish assemblages worldwide          下载免费PDF全文

Sofia Henriques  François Guilhaumon  Sébastien Villéger  Sandra Amoroso  Susana França  Stéphanie Pasquaud  Henrique N Cabral  Rita P Vasconcelos 《Fish and Fisheries》2017,18(4):752-771

Assessing trait–environment relationships is crucial for predicting effects of natural and human‐induced environmental change on biota. We compiled a global database of fish assemblages in estuaries, functional traits of fishes and ecosystem features of estuaries. And we quantified the relative importance of ecosystem features as drivers of patterns of fish functional traits among estuaries worldwide (i.e. drivers of the proportions of fish traits). In addition to biogeographical context, two main environmental gradients regulate traits patterns: firstly temperature, and secondly estuary size and hydrological connectivity of the estuary with the marine ecosystem. Overall, estuaries in colder regions, with larger areas and with higher hydrological connectivity with the marine ecosystem, have higher proportions of marine fish (versus freshwater), macrocarnivores and planktivores (versus omnivores, herbivores and detritivores) and larger fish, with greater maximum depth of distribution and longer lifespan. The observed trait patterns and trait–environment relationships are likely generated by multiple causal processes linked to physiological constraints due to temperature and salinity, size‐dependent biotic interactions, as well as habitat availability and connectivity. Biogeographical context and environmental conditions drive species richness and composition, and present results show that they also drive assemblage traits. The observed trait patterns and trait–environment relationships suggest that assemblage composition is determined by the functional role of species within ecosystems. Conservation strategies should be coordinated globally and ensure protection of an array of estuaries that differ in ecosystem features, even if some of those estuaries do not support high species richness.  相似文献   

Leukaemic neoplasia in free-living mammals in Denmark     

K Elvestad  U V Henriques 《Acta veterinaria Scandinavica》1985,26(1):61-71

In a material of free-living mammals collected and necropsied during the period 1934–1984, leukaemic neoplasia was found in 1 roe deer, 1 red deer and 15 brown hares. In order to determine the types of leukaemia, a histological reexamination of the tissues was performed. The two deer had the same type, while 4 different types were found in the hares. There was no evidence suggesting a contagious nature of the neoplasia or an accumulation of cases within certain geographic areas.  相似文献   

Sublethal effects of propiconazole on the metabolism of lambari Deuterodon iguape (Eigenmann 1907), a native species from Brazil     

Henriques  Marcelo Barbosa  Rezende  Karina Fernandes Oliveira  Castilho-Barros  Leonardo  Barbieri  Edison 《Fish physiology and biochemistry》2021,47(4):1165-1177

Fish Physiology and Biochemistry - The objective of this study was to analyze the sublethal effects of propiconazole on Deuterodon iguape, a native fish common in Brazil, which has potential for...  相似文献   

Canine lymphoma and vector‐borne diseases: Molecular and serological evaluation of a possible complicity     

Joaquim Henriques  Ricardo Felisberto  Bruno Almeida  Joana Ramos  Fernando Constantino‐Casas  Jane Dobson  Raquel Matos  Ana Santos  Rita de Sousa  Margarida Alves 《Veterinary and comparative oncology》2021,19(1):183-190

Lymphoma is the most common haematological malignancy in dogs and its aetiology is largely unknown. The presence of canine vector‐borne agents (CVBD) in lymphoma tissues has been described and its causative effects questioned. We intended to evaluate the presence and extent of Leishmania infantum, Ehrlichia canis, Anaplasma phagocytophilum and Bartonella henselae infection in dogs with lymphoma. Sixty‐one dogs, living in the Lisbon metropolitan area, with a diagnosis of lymphoma were enrolled. Immunofluorescence assays were used to detect serum IgG's. The presence of DNA from CVBD agents in tumour tissue was assessed by PCR. All dogs tested negative for B. henselae, A. phagocytophilum and E. canis by both serology and PCR. Regarding L. infantum, 8.2% (n = 5) of the dogs had a positive serologic result. L. infantum DNA was detected in two samples of diffuse large B‐cell lymphoma (DLBCL). These results show an increased, but not significant, seropositivity (8.2% vs 7.9%) and molecular detection (3.3% vs 1.2%) for L. infantum in dogs with lymphoma, when compared to the reported canine population in the same geographical area. We could not identify an association between lymphoma and E. canis, A. phagocytophilum, B. henselae or Leishmania infantum infection in the studied population. Nevertheless, further studies, following dogs trough their CVBD disease evolution, are worthwhile and may help clarify a possible role of CVBD agents in lymphomagenesis.  相似文献   

Taking stock of the assisted migration debate   总被引：1，自引：0，他引：1  

N. Hewitt  N. Klenk  A.L. Smith  D.R. Bazely  N. Yan  S. Wood  J.I. MacLellan  C. Lipsig-Mumme  I. Henriques 《Biological conservation》2011,144(11):2560-2572

Assisted migration was proposed several decades ago as a means of addressing the impacts of climate change on species populations. While its risks and benefits have been debated, and suggestions for planning and management given, there is little consensus within the academic literature over whether to adopt it as a policy. We evaluated the main features of the assisted migration literature including the study methods, taxonomic groups, geographic regions and disciplines involved. We further assessed the debate about the use of assisted migration, the main barriers to consensus, and the range of recommendations put forth in the literature for policy, planning or implementation. Commentaries and secondary literature reviews were as prevalent as first-hand scientific research and attention focussed on a global rather than regional level. There was little evidence of knowledge transfer outside of the natural sciences, despite the obvious policy relevance. Scholarly debate on this topic has intensified during the last 3 years. We present a conceptual framework for evaluating arguments in the debate, distinguishing among the direct risks and benefits to species, ecosystems and society on the one hand, and other arguments regarding scientific justification, evidence-base and feasibility on the other. We also identify recommendations with potential to advance the debate, including careful evaluation of risks, benefits and trade-offs, involvement of relevant stakeholders and consideration of the complementarity among assisted migration and less risk-tolerant strategies. We conclude, however, that none of these will solve the fundamental, often values-based, challenges in the debate. Solutions are likely to be complex, context-dependent and multi-faceted, emerging from further research, discussion and experience.  相似文献   

Kinetics of Mercury Accumulation and Its Effects on Ulva lactuca Growth Rate at Two Salinities and Exposure Conditions     

S??nia Costa  Daniel Crespo  Bruno M. G. Henriques  Eduarda Pereira  Armando C. Duarte  Miguel A. Pardal 《Water, air, and soil pollution》2011,217(1-4):689-699

The research on metals effect on macroalgae has been focused on Cd, Cu, Zn and Pb, and no study dealt with the effects of mercury on macroalgae growth rate. Also, the kinetic of accumulation is not well known. The main aim of this work was to assess the mercury accumulation kinetics of Ulva lactuca and its effects on growth rate. Three concentrations were tested: 5, 50 and 500???g?L^?1, under static and renewal conditions and at two salinities (15 and 35), during 72?h. The mercury accumulation kinetic patterns were different according to the exposure conditions and metal concentration, but were always a very fast process (hours). Three patterns were established based on the models substrate inhibition, linear regression and Michaelis?CMenten equation. Statistical differences in the mercury accumulated were recorded depending on the salinity values and exposure conditions, being higher at salinity 15 and at renewal tests, corresponding to the lower relative growth rates. The lowest mercury concentration did not have an effect on relative growth rate, while the others caused an accentuated inhibition at 24?h. The highest concentration was toxic to algae causing its death before 48?h. Under controlled conditions, the U. lactuca??s high and fast ability for mercury accumulation could be useful for phytoremediation and for industrial wastewaters treatment.  相似文献   

Detection of convective downflows in a sunspot penumbra     

Scharmer GB  Henriques VM  Kiselman D  de la Cruz Rodríguez J 《Science (New York, N.Y.)》2011,333(6040):316-319

The fine structure and dynamics of sunspots and the strong outflow in their outer filamentary part--the penumbra--have puzzled astronomers for more than a century. Recent theoretical models and three-dimensional numerical simulations explain the penumbral filaments and their radiative energy output as the result of overturning convection. Here, we describe the detection of ubiquitous, relatively dark downward flows of up to 1 kilometer per second (km/s) in the interior penumbra, using imaging spectropolarimetric data from the Swedish 1-meter Solar Telescope. The dark downflows are omnipresent in the interior penumbra, distinguishing them from flows in arched flux tubes, and are associated with strong (3 to 3.5 km/s) radial outflows. They are thus part of a penumbral convective flow pattern, with the Evershed flow representing the horizontal component of that convection.  相似文献