Impact of postoperative antibiotics on rates of infection and implant removal after tibial tuberosity advancement in 1,768 canine stifles     

Christopher L. Ferrell  Matthew D. Barnhart  Eric Herman 《Veterinary surgery : VS》2019,48(5):694-699

  相似文献   

Characterization of virulence properties of Listeria monocytogenes serotype 4b strains of different origins     

Werbrouck H  Botteldoorn N  Ceelen L  Decostere A  Uyttendaele M  Herman L  Van Coillie E 《Zoonoses and public health》2008,55(5):242-248

In this study, the virulence heterogeneity of Listeria monocytogenes serotype 4b strains of different origins was analysed on different levels. On one hand, the survival of L. monocytogenes strains in synthetic gastric fluid was studied. On the other hand, the pathogenic potential of strains with different inlB expression levels was analysed in an A/J mouse model for gastrointestinal listeriosis. Differences in survival capacity in gastric fluid and in in vivo virulence potential were observed between the tested strains. No clear correlation between the origin and the obtained data could be made. However, these results confirm the existence of heterogeneity in virulence potential of L. monocytogenes serotype 4b strains.  相似文献   

Plasma and cerebrospinal fluid interleukin-1β during lipopolysaccharide-induced systemic inflammation in ewes implanted or not with slow-release melatonin     

Janina Skipor  Marta Kowalewska  Aleksandra Szczepkowska  Anna Majewska  Tomasz Misztal  Marek Jalynski  Andrzej P.Herman  Katarzyna Zabek 《畜牧与生物技术杂志(英文版)》2018,(1)

Background: Interleukin-1β(IL-1β) is important mediator of inflammatory-induced suppression of reproductive axis at the hypothalamic level. At the beginning of inflammation, the main source of cytokines in the cerebrospinal fluid(CSF) is peripheral circulation, while over time, cytokines produced in the brain are more important. Melatonin has been shown to decrease pro-inflammatory cytokines concentration in the brain. In ewes, melatonin is used to advance the onset of a breading season. Little is known about CSF concentration of IL-1β in ewes and its correlation with plasma during inflammation as well as melatonin action on the concentration of IL-1β in blood plasma and the CSF, and brain barriers permeability in early stage of lipopolysaccharide(LPS)-induced inflammation.Methods: Systemic inflammation was induced through LPS administration in melatonin-and sham-implanted ewes. Blood and CSF samples were collected before and after LPS administration and IL-1β and albumin concentration were measured. To assess the functions of brain barriers albumin quotient(QAlb) was used.Expression of IL-1β(Il1B) and its receptor type Ⅰ(Il1r1) and type Ⅱ(Il1r2) and matrix metalloproteinase(Mmp) 3 and 9 was evaluated in the choroid plexus(CP).Results: Before LPS administration, IL-1β was on the level of 62.0 ± 29.7 pg/mL and 66.4 ± 32.1 pg/mL in plasma and 26.2 ± 5.4 pg/mL and 21.3 ± 8.7 pg/mL in the CSF in sham-and melatonin-implanted group, respectively.Following LPS it increased to 159.3 ± 53.1 pg/mL and 197.8 ± 42.8 pg/mL in plasma and 129.8 ± 54.2 pg/mL and139.6 ± 51.5 pg/mL in the CSF. No correlations was found between plasma and CSF IL-1β concentration after LPS in both groups. The QAlb calculated before LPS and 6 h after was similar in all groups. Melatonin did not affected m RNA expression of Il1B, Il1r1 and Il1r2 in the CP. The m RNA expression of Mmp3 and Mmp9 was not detected.Conclusions: The lack of correlation between plasma and CSF IL-1β concentration indicates that at the beginning of inflammation the local synthesis of IL-1β in the CP is an important source of IL-1β in the CSF. Melatonin from slow-release implants does not affect IL-1β concentration in plasma and CSF in early stage of systemic inflammation.  相似文献   

Life history data of the rosy apple aphid<Emphasis Type="Italic"> Dysaphis plantaginea</Emphasis> (Pass.) (Homopt., Aphididae) on plantain and as migrant to apple     

L.?H.?M.?Blommers  H.?H.?M.?HelsenEmail author  F.?W.?N.?M.?Vaal 《Journal of pest science》2004,77(3):155-163

The rosy apple aphid Dysaphis plantaginea (Passerini) is a key pest in western European apple orchards; the economic damage threshold is so low that outbreaks cannot be forecasted. A mass rearing of the species on plantain (Plantago lanceolata L.) was initiated, with the aim to infest apple trees with either the autumn migrants, gynoparae and males, or the egg-laying females (oviparae). Here, data are presented about the propagation of the species on plantain, on the production of autumn migrants under laboratory conditions, and on the duration of juvenile development and reproductive capacities of both gynoparae and oviparae. Under long-day conditions (18 h light/day) on plantain, the thermal constant for the duration of juvenile development was 166 dd (day-degrees) above the lower development threshold of 5.1 °C, and daily larviposition amounted to 1.87 times the temperature (°C)minus 0.8, above a lower threshold of 4.3 °C. Between 32 and 36 larvae were produced by the young female before the first larvae become adult and their reproduction started to overshadow their mothers. A plant freshly infested with 12 reproducing females and transferred to short-day conditions (12 h light/day) yielded up to 5,000 autumn migrants, with males in the majority. The first gynoparae appeared after about 25 days at both 16 and 20 °C, and males appeared after 40 and 33 days, respectively. Young adult gynoparae produced most of their about ten offspring right after landing on apples, unless temperature was well below 15 °C. The duration of juvenile development of these oviparae appeared to be rather variable and their egg-laying so protracted that each of these females needs to survive several weeks to produce a handful of winter eggs. With reproductive capacities of up to 14 progeny/female for gynoparae and 7.4 for oviparae, release of one gynopara in the field could theoretically lead to the deposition of 100 winter eggs.  相似文献   

Giardia duodenalis and Cryptosporidium spp. in a veterinary college bovine teaching herd     

Uehlinger FD  Barkema HW  Dixon BR  Coklin T  O'Handley RM 《Veterinary parasitology》2006,142(3-4):231-237

In a preliminary study, we commonly identified Giardia duodenalis in adult dairy cattle from a veterinary college teaching herd. Therefore, the present study was carried out in order to better understand the potential of adult cattle to act as a source for G. duodenalis infections for students and staff at the veterinary college. Fecal samples were collected bi-weekly from this herd of adult cattle (n=30) over an 8-month period to determine the prevalence of G. duodenalis and Cryptosporidium spp. within the herd. Nested PCR followed by DNA sequencing was then performed on a subset of positive samples in order to better understand the zoonotic potential of these infections. Every cow was sampled between 11 and 18 times, depending on the date the animal joined the teaching herd. In total, 507 fecal samples were collected from 30 different cows and examined for cysts and oocysts using epifluorescence microscopy. G. duodenalis prevalence during the course of the study ranged from 37% (11/30) to 64% (18/28), with a mean of 49%. Cumulative G. duodenalis prevalence was 73% (22/30). Zoonotic G. duodenalis assemblage A genotype was identified in 43% (6/14) of the G. duodenalis-positive samples on which PCR and genetic sequencing were successfully performed. G. duodenalis assemblage E was identified in 57% (8/14) of these samples. Cryptosporidium spp. oocysts were not detected in the feces of any cows during the study period. The presence of the zoonotic G. duodenalis assemblage A in 43% of the sequenced samples indicates that there is a potential risk of infection for students and staff at this research and teaching facility, although the roles of cows as sources of giardiasis in humans remain uncertain. Furthermore, due to the large amount of feces they produce, adult cattle may serve as important sources for G. duodenalis infections in young cattle, or other animals in the facility, despite relatively low numbers of cysts excreted per gram of feces. In contrast, the results of this study indicate that this herd posed a negligible risk of transmitting Cryptosporidium parvum infections to humans.  相似文献   

Cell type-specific resistance of trigeminal ganglion neurons towards apoptotic stimuli     

Geenen K  Favoreel HW  Nauwynck HJ 《Veterinary microbiology》2006,113(3-4):223-229

Trigeminal ganglion (TG) neurons are important target cells for many alphaherpesviruses and constitute a major site of virus latency and reactivation. Earlier we showed that porcine TG neurons are remarkably more resistant towards (apoptotic) cell death resulting from infection by the swine alphaherpesvirus pseudorabies virus (PRV) compared to a broad range of other primary porcine cell types and that this resistance does not depend on the strongly anti-apoptotic US3 viral protein kinase (Geenen, K., Favoreel, H.W., Nauwynck, H.J., 2005a. Higher resistance of porcine trigeminal ganglion neurons towards pseudorabies virus-induced cell death compared with other porcine cell types in vitro. J. Gen. Virol. 86, 1251-1260). Although other viral anti-apoptotic proteins may be involved in survival of TG neurons during PRV infection, an additional factor may be that TG neurons possess a cell type-dependent capacity to withstand apoptosis compared to other cell types. To investigate this, we treated uninfected porcine TG cultures, swine kidney cells, and porcine superior cervical ganglion (SCG) neurons with several apoptosis-inducing reagents (staurosporine, camptothecin and genistein). None of these reagents were able to trigger substantial apoptotic cell death in TG neurons, whereas non-neuronal TG cells, swine kidney cells, and SCG neurons showed a clear dose-dependent increase in apoptosis using either of these reagents. In conclusion, sensory TG neurons may contain a cell type-specific capacity to withstand different apoptotic assaults, including infection with an alphaherpesvirus.  相似文献   

Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity     

Van Waeyenberghe L  Pasmans F  D'Herde K  Ducatelle R  Favoreel H  Li SJ  Haesebrouck F  Martel A 《Veterinary research》2012,43(1):32

ABSTRACT: Although aspergillosis is one of the most common diseases in captive birds, the pathogenesis of avian aspergillosis is poorly known. We studied the role of avian respiratory macrophages as a first line of defense against avian aspergillosis. The phagocytic and killing capacities of avian respiratory macrophages were evaluated using pigeon respiratory macrophages that were inoculated with Aspergillus fumigatus conidia. On average, 25% of macrophage-associated conidia were phagocytosed after one hour. Sixteen percents of these cell-associated conidia were killed after 4 h and conidial germination was inhibited in more than 95% of the conidia. A. fumigatus conidia were shown to be cytotoxic to the macrophages. Intracellularly germinating conidia were located free in the cytoplasm of necrotic cells, as shown using transmission electron microscopy. These results suggest that avian respiratory macrophages may prevent early establishment of infection, unless the number of A. fumigatus conidia exceeds the macrophage killing capacity, leading to intracellular germination and colonization of the respiratory tract.  相似文献   

Occurrence of Cryptosporidium and Giardia on beef farms and water sources within the vicinity of the farms on Prince Edward Island, Canada     

Budu-Amoako E  Greenwood SJ  Dixon BR  Barkema HW  McClure JT 《Veterinary parasitology》2012,184(1):1-9

The objectives of this study were to determine the prevalence and assemblages of Giardia and species of Cryptosporidium on beef farms in Prince Edward Island (PEI), Canada, including the water sources associated with the farms, and to determine risk factors for infection of cattle with these parasites. Twenty beef farms were selected based on the presence of surface water < 500 m from the barn. Prevalence was determined by direct immunofluorescence microscopy, while genotyping and species determination were performed by nested-PCR and DNA sequencing. Giardia was detected in 42% (95% CI: 38-46%) of fecal samples from 100% farms while Cryptosporidium was detected in 17% (95% CI: 14-19%) of fecal samples from 80% of farms. The most predominant Giardia assemblage isolated was the livestock specific assemblage E (89%). The zoonotic assemblages A and B were found in 4 and 7% of the Giardia isolates that were genotyped, respectively. The Giardia assemblages were detected equally between the cows and calves examined. Overall, the most common Cryptosporidium species detected in this study was Cryptosporidium andersoni (49%), predominantly found in cattle >6 mo of age, while most Cryptosporidium bovis and Cryptosporidium pestis (previously Cryptosporidium parvum ‘bovine genotype’) isolates were detected in calves ≤ 6 mo of age. All Cryptosporidium ryanae isolates (four) were found in calves. Giardia cysts and Cryptosporidium oocysts were detected in 14 and 93% of surface water samples of 14 farms, respectively. Cryptosporidium oocysts were detected in three (15%) ground water samples of 20 farms. One Cryptosporidium-positive water sample, which was the only surface water sample amenable to genotyping, contained C. parvum. The farm-level risk factors investigated in this study, age of animals and location of the farm, were not associated with the risk of infection in cattle with either Cryptosporidium spp. or Giardia duodenalis.We conclude that beef cattle are a potential reservoir of Cryptosporidium spp. and G. duodenalis that could contaminate source water. There is the possibility of further transmission to humans on PEI if the source water is not properly treated prior to consumption.  相似文献   

Bordetella bronchiseptica infection in cats. ABCD guidelines on prevention and management     

Herman Egberink  Diane Addie  Sándor Belák  Corine Boucraut-Baralon  Tadeusz Frymus  Tim Gruffydd-Jones  Katrin Hartmann  Margaret J. Hosie  Albert Lloret  Hans Lutz  Fulvio Marsilio  Maria Grazia Pennisi  Alan D. Radford  Etienne Thiry  Uwe Truyen  Marian C. Horzinek 《Journal of Feline Medicine and Surgery》2009,11(7):610-614

  相似文献   

Motivation for additional water use of growing-finishing pigs     

Herman M. Vermeer  Nienke Kuijken  Hans A.M. Spoolder 《Livestock Science》2009,124(1-3):112-118

  相似文献