Increase in plasma total antioxidant capacity of grazing Japanese Black heifers and cows in forestland in Japan     

Satoshi Haga  Hiroshi Ishizaki  Miwa Nakano  Seiji Nakao  Kiyoshi Hirano  Yoshito Yamamoto  Miya Kitagawa  Hiroyuki Sasaki  Yoshihiro Kariya 《Animal Science Journal》2014,85(2):135-142

Blood total antioxidant capacity (TAC) has become a key bio‐marker for animal health. Forest‐grazing cattle are known to forage various native plants that have high TAC. This study evaluated differences of plasma TAC between forest‐grazing (FG) and pasture‐grazing cattle (PG). Experiment 1 monitored the plasma TAC levels of 32 Japanese Black cattle. The level in PG did not change throughout the grazing period. However, that in FG, which increased from summer, was significantly higher than that in PG through fall (P < 0.05). In experiment 2, we used nine Japanese Black heifers and investigated their blood antioxidant parameters and the TAC in plants that the cattle consumed in late June and September. The plasma TAC levels in FG were significantly higher than those in PG in both periods (P < 0.05). Plasma levels of lipid peroxidation in FG tended to be lower than that in PG (P = 0.098). Furthermore, the TAC levels in various species of shrubs and trees consumed by FG were higher than those in pasture grasses. Results of this study show that plasma TAC of grazing Japanese Black cattle in forestland increase from summer through fall.  相似文献   

Changes in seawater tolerance during the development of eyed-stage embryos in shishamo smelt Spirinchus lanceolatus (Hikita)     

Shinya Mizuno  Yoshitaka Sasaki  & Kazushi Imada 《Aquaculture Research》2005,36(6):615-619

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Predation by scavenging amphipods to injured hatchery-raised juvenile Japanese flounder Paralichthys olivaceus under laboratory conditions     

Keiichiro Ide  Kazutaka Takahashi  Koichi Sasaki  Michio Omori 《Fisheries Science》2006,72(6):1209-1214

ABSTRACT:   The attacking potential of the scavenging amphipod Scopelocheirus onagawae on artificially injured hatchery-raised Japanese flounder Paralichthys olivaceus juveniles was investigated in relation to the degree of injury on the fish. All injured flounder juveniles were attacked by amphipods regardless of the degree of injury, while non-injured juveniles were not attacked. The attack by amphipods on the juveniles generally depended on the amount of glycine, a main feeding stimulant for the amphipod, released from the injury opening. The swimming ability of flounder juveniles was important to avoid the attack of amphipods. Furthermore, an area of injury allowing the amphipods to cling to the fish affects to the vulnerability of juveniles against the predation of amphipods. This study suggests that scavenging amphipods are potentially involved in the rapid reduction of the number of hatchery-raised juveniles.  相似文献   

Programmed Cell Death Induced by (?)-8,9-Dehydroneopeltolide in Human Promyelocytic Leukemia HL-60 Cells under Energy Stress Conditions     

Haruhiko Fuwa  Mizuho Sato  Makoto Sasaki 《Marine drugs》2014,12(11):5576-5589

(+)-Neopeltolide is a marine macrolide natural product that exhibits potent antiproliferative activity against several human cancer cell lines. Previous study has established that this natural product primarily targets the complex III of the mitochondrial electron transport chain. However, the biochemical mode-of-actions of neopeltolide have not been investigated in detail. Here we report that (−)-8,9-dehydroneopeltolide (8,9-DNP), a more accessible synthetic analogue, shows potent cytotoxicity against human promyelocytic leukemia HL-60 cells preferentially under energy stress conditions. Nuclear morphology analysis, as well as DNA ladder assay, indicated that 8,9-DNP induced significant nuclear condensation/fragmentation and DNA fragmentation, and these events could be suppressed by preincubating the cells with a pan-caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (zVAD). Immunoblot analysis demonstrated the release of cytochrome c from the mitochondria and the cleavage of full-length caspase-3 and poly(ADP-ribose) polymerase (PARP). These results indicated that 8,9-DNP induced caspase-dependent apoptotic programmed cell death under energy stress conditions. It was also found that 8,9-DNP induced non-apoptotic cell death in the presence/absence of zVAD under energy stress conditions. Immunoblot analysis showed the intracytosolic release of apoptosis-inducing factor (AIF), although it did not further translocate to the nucleus. It appears most likely that, in the presence of zVAD, 8,9-DNP triggered necrotic cell death as a result of severe intracellular ATP depletion.  相似文献   

Presumptive acute lung injury following multiple surgeries in a cat     

Masaaki Katayama  Yasuhiko Okamura  Rieko Katayama  Jun Sasaki  Shunsuke Shimamura  Yuji Uzuka  Hiroaki Kamishina  Yoshinori Nezu 《The Canadian veterinary journal. La revue veterinaire canadienne》2013,54(4):381-386

A 12-year-old, 3.5-kg spayed female domestic shorthair cat had a tracheal mass identified as malignant B-cell lymphoma. The cat had tracheal resection and subsequently developed laryngeal paralysis. Due to multiple episodes of respiratory distress the cat subsequently had tracheal surgeries. Finally, the cat had a sudden onset of severe respiratory distress and collapsed. Computed tomography imaging and arterial blood gas analysis supported a diagnosis of acute lung injury.  相似文献   

Laboratory test results in artificial model of caval syndrome in canine heartworm disease     

H Kitagawa  Y Sasaki  K Ishihara  Y Kuwahara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(5):1123-1125

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Induction of alimentary diabetes and insulin responses to glucose in Microtus arvalis Pallas     

T Arai  M Sasaki  Y Oki  K Komeda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1985,47(4):603-610

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Inhibitory effects of tumor necrosis factor (TNF) and its combination with interferons on the syncytium formation by bovine leukemia virus (BLV) and on the multiplication of BLV     

H Ike  O Sasaki  A Yamada  J Imanishi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(4):869-871

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Establishment of an attenuated strain of bovine respiratory syncytial virus for live virus vaccine   总被引：1，自引：0，他引：1  

M Kubota  S Fukuyama  K Kodama  N Sasaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(4):695-703

To develop a live virus vaccine for the prevention of bovine respiratory syncytial (BRS) virus infection in calves, an attempt was made to produce an attenuated virus. The RS-52 strain of BRS virus, isolated from the nasal secretions of a naturally infected calf, was subjected to serial passages in adult hamster lung established (HAL) cells at 30 degrees C and the attenuated rs-52 strain as a live virus vaccine was established. The rs-52 strain multiplied better at 30 degrees C than at 34 or 37 degrees C in HAL cells. The differences in the highest virus titers of this strain between the culture temperature of 30 degrees C and that of 34 or 37 degrees C were more than 2.25 log TCID50. Colostrum-deprived newborn calves and 2 approximately 4 months old calves inoculated with the rs-52 strain manifested no abnormal clinical sings at all. However, all inoculated calves produced serum neutralization antibody. When the colostrum-deprived newborn calves immunized with the rs-52 strain were challenged with the virulent NMK7 strain of BRS virus, they exhibited no pyrexia or other abnormal clinical signs at all. An attempt was made to recover the virus from nasal secretions of these calves, but in vain. On the other hand, a nonimmunized control colostrum-deprived newborn calf developed slight fever, mild cough, and slight serous nasal discharge after challenge exposure. The virus was recovered from nasal secretions of this calf. From these results, it was considered that the rs-52 strain could be used as an attenuated live virus vaccine for prevention of BRS virus infection.  相似文献   

Restriction fragment length polymorphism for the Yc subunit gene of rat liver glutathione S-transferase     

Y Sasaki  M Hayashi  K Matsumoto  S Namioka 《The Japanese journal of veterinary research》1990,38(2):35-42

An altered expression of the Yc subunit gene of rat glutathione S-transferase (GST) in the liver of the LEC rat, which is a mutant strain with spontaneous hereditary hepatitis associated with severe jaundice, has been reported. To provide further information concerning the structure of the Yc subunit gene, we carried out the Southern blot hybridization analysis of DNA samples from rats of eight different inbred strains including LEC with cDNA complementary to mRNA specific for the Yc subunit of rat liver GST as a probe. The hybridization patterns of the DNA samples from rats belonging to the different inbred strains showed interstrain variation in the length of restriction fragments with four restriction endonucleases. Since the DNA samples prepared from several rats of one inbred strain gave an identical hybridization pattern, the restriction fragment patterns for the Yc gene could be used as markers for genetic monitoring of inbred rat strains. Although the altered expression of Yc-Yc activity of GST has been observed in the liver of the LEC rat, the characteristic changes in the gene structure of the Yc subunit of LEC rat were not detected in the present hybridization analysis.  相似文献