Genetic diversity of the entomopathogen<Emphasis Type="Italic">Verticillium lecanii</Emphasis> on the basis of vegetative compatibility     

Midori?Sugimoto  Masanori?KoikeEmail author  Hideyuki?Nagao  Kayo?Okumura  Masayuki?Tani  Katsuhisa?Kuramochi 《Phytoparasitica》2003,31(5):450-457

Forty-three isolates ofVerticillium lecanii from insects, phytopathogenic fungi and other substrates were tested for vegetative compatibility by observing heterokaryon formation among complementary nitrate-nonutilizing (nit) mutants.nit mutants were isolated from 42/43 strains examined. Twenty-one isolates were self-incompatible, and the remaining 21 isolates were divided into 14 vegetative compatibility groups (VCGs): ten containing only a single strain each, and the remaining four containing two to four isolates each. Members of isolates in each of these VCGs all shared the same IGS haplotype. Further, the isolates within a VCG were correlated with one another in part by fragment patterns of mt-LrDNA, -SrDNA, Bt-2 and H4 region, by PCR-RFLP and -SSCP, but not by dsRNA. Two isolates belonging to VL-J2 have high virulence to aphids, whereas strains from VL-J1 lack this character. These findings indicate that two VCGs (VL-J1 and -J2) may originate from two distinct clonal lineages. Alternatively, high VCG diversity and HSI frequency ofV. lecanii might be associated with an array of distinct lineages. These data not only suggest relationships among DNA polymorphisms, virulence, and VCG, but also demonstrate genetic heterogeneity ofV. lecanii. http://www.phytoparasitica.org posting Sept. 30, 2003.  相似文献   

Nitrate reductase,a nitric oxide-producing enzyme: induction by pathogen signals   总被引：8，自引：1，他引：7  

Ayako?Yamamoto  Shinpei?Katou  Hirofumi?Yoshioka  Noriyuki?Doke  Kazuhito?KawakitaEmail author 《Journal of General Plant Pathology》2003,69(4):218-229

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Molecular Epidemiology of Paramyxoviruses in Frugivorous Eidolon helvum Bats in Zambia     

Walter MULEYA  Michihito SASAKI  Yasuko ORBA  Akihiro ISHII  Yuka THOMAS  Emiko NAKAGAWA  Hirohito OGAWA  Bernard HANG’OMBE  Boniface NAMANGALA  Aaron MWEENE  Ayato TAKADA  Takashi KIMURA  Hirofumi SAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):611-614

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稻瘟病菌致病性遗传研究初报——云南省稻瘟病菌有性世代研究之五   总被引：1，自引：0，他引：1  

李成云  藤田佳克 《云南农业大学学报(自然科学版)》1993,8(3):154-157

 本文报道龙爪稷分离菌G10-1马水稻分离菌后代菌性2145-R-57-1交配产生的同一子囊中分离出的子囊孢子致病性及交配型分离情况.交配型分离比1:1;但致病性分离情况比较复杂.很多子囊孢子菌株失去亲本菌株对供试水稻和龙爪稷的致病性,少数菌株获得亲本菌株所没有的致病性.对稻瘟病菌致病性的遗传尚需作大量分析工作.  相似文献   

A new source of basaltic meteorites inferred from Northwest Africa 011     

Yamaguchi A  Clayton RN  Mayeda TK  Ebihara M  Oura Y  Miura YN  Haramura H  Misawa K  Kojima H  Nagao K 《Science (New York, N.Y.)》2002,296(5566):334-336

Eucrites are a class of basaltic meteorites that share common mineralogical, isotopic, and chemical properties and are thought to have been derived from the same parent body, possibly asteroid 4 Vesta. The texture, mineralogy, and noble gas data of the recently recovered meteorite, Northwest Africa (NWA) 011, are similar to those of basaltic eucrites. However, the oxygen isotopic composition of NWA011 is different from that of other eucrites, indicating that NWA011 may be derived from a different parent body. The presence of basaltic meteorites with variable oxygen isotopic composition suggests the occurrence of multiple basaltic meteorite parent bodies, perhaps similar to 4 Vesta, in the early solar system.  相似文献   

Muscle fibre distribution in forelimb,hindlimb and trunk muscles in three bat species: The little Japanese horseshoe,greater horseshoe and Egyptian fruit     

Claudius Luziga  Hirofumi Miyata  Hiroshi Nagahisa  Takashi Oji  Naomi Wada 《Anatomia, histologia, embryologia》2021,50(4):685-693

This study characterised muscle fibres in trunk, forelimb and hindlimb muscles of three bat species: little Japanese horseshoe (Rhinolophus cornutus), greater horseshoe (Rhinolophus ferrumequinum) and Egyptian fruit (Rousettus aegyptiacus). Twenty-seven muscles from trunk, forelimb and hindlimb were dissected, weighed and analysed by immunohistochemistry and sodium didecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and determined their cross-sectional areas (CSA). Results showed that Type IIa and Type IIa/x made the highest proportion of total muscle mass. Moderate proportion was formed by Type IIb. Type I and IIx appeared at very low levels in all bats. Type IIb was the only fibre type detected in patagial muscles in wing membrane of greater horseshoe while other fibre types were not observed. Type I muscle fibres were very few and appeared infrequently in fifteen muscles of Egyptian fruit and in only one muscle in each, greater horseshoe and little Japanese horseshoe. Type IIx was also detected in three muscles in greater horseshoe and only one muscle in Egyptian fruit but none in little Japanese horseshoe. The highest average CSA μm² was detected in Type IIb and values were 734.2μm² for LHB; 1537.9μm² for GHB and 1,720.9μm² for EFB. Lowest and undetermined values were observed for Type I and IIx. These data demonstrate that Type IIa, IIa/x and IIb form significant proportion of adult bat muscle mass and Type IIb is the largest fibre type. The distribution pattern is suggestive of specialised functions of the fibres in relation to orientation and speed of bats during flight.  相似文献   

Databases for technical aspects of immunohistochemistry: 2021 update     

Takayasu Moroki  Saori Matsuo  Hirofumi Hatakeyama  Seigo Hayashi  Izumi Matsumoto  Satoshi Suzuki  Takashi Kotera  Kazuyoshi Kumagai  Kiyokazu Ozaki 《Journal of toxicologic pathology》2021,34(2):161

With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) as well as the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, blocking condition, secondary antibody information, etc.) for a total of 509 primary antibodies (comprising 220 different types). These survey results were an update on the contents reported by CEAH in 2017.  相似文献   

Effects of low and high levels of maternal nutrition consumed for the entirety of gestation on the development of muscle,adipose tissue,bone, and the organs of Wagyu cattle fetuses     

Yi Zhang  Kounosuke Otomaru  Kazunaga Oshima  Yuji Goto  Ichiro Oshima  Susumu Muroya  Mitsue Sano  Rena Saneshima  Yukiko Nagao  Aoi Kinoshita  Yasuko Okamura  Sanggun Roh  Akira Ohtsuka  Takafumi Gotoh 《Animal Science Journal》2021,92(1):e13600

This study aimed to investigate the effects of high and low levels of energy intake during the entire gestation period on the skeletal muscle development, organ development, and adipose tissue accumulation in fetuses of Wagyu (Japanese Black) cows, a breed with highly marbled beef. Cows were allocated to a high-nutrition (n = 6) group (fed 120% of the nutritional requirement) or low-nutrition (n = 6) group (fed 60% of the nutritional requirement). The cows were artificially inseminated with semen from the same sire, and the fetuses were removed by cesarean section at 260 ± 8.3 days of fetal age and slaughtered. The whole-body, total muscle, adipose, and bone masses of the fetal half-carcasses were significantly higher in the high-nutrition group than the low-nutrition group (p = 0.0018, 0.009, 0.0004, and 0.0362, respectively). Fifteen of 20 individual muscles, five of six fat depots, nine of 17 organs, and seven of 12 bones that were investigated had significantly higher masses in the high-nutrition group than the low-nutrition group. The crude components and amino acid composition of the longissimus muscle significantly differed between the low- and high-nutrition groups. These data indicate that maternal nutrition during gestation has a marked effect on the muscle, bone, and adipose tissue development of Wagyu cattle fetuses.  相似文献   

Experimental studies on SO2 injuries in higher plants III: Inhibitory effect of suite ion on 14 CO 2 fixation     

Hirofumi Tanaka  Toshiharu Takanashi  Michihiko Yatazawa 《Water, air, and soil pollution》1974,3(1):11-16

Photosynthesis decreases reversibly in plants exposed to SO₂. Photosynthesis recovers when the exposure to SO₂ is discontinued. Inactivation of a photosynthetic enzyme, ribulose-1,5-diphosphate carboxylase, by sulfonation of its SH groups was investigated as a cause of the reversible reduction of photosynthesis. The relationship between the sulfite ion concentration in the reaction mixture and ¹⁴CO₂ fixation catalized by the enzyme which was prepared from alfalfa leaves was explored by using radioactive NaHCO₃. About 50% and 85% inhibitions of ¹⁴CO₂ fixation were observed at 3 × 10^?3 M and 3 × 10^?2 M concentration of sulfite ion in the reaction mixture, respectively. The accumulation of 3 × 10^?4 M sulfite ion on the reaction site of the enzyme involved in the plants which were exposed to SO₂ could considerably reduce the CO₂ assimilation of the plant.  相似文献   

Effect of oral administration of a single bolus of six different protein sources on digestive physiology of red seabream Pagrus major juveniles     

Murashita  Koji  Takakuwa  Fumiaki  Matsunari  Hiroyuki  Yoshinaga  Hazuki  Yamamoto  Takeshi  Oku  Hiromi  Furuita  Hirofumi 《Fish physiology and biochemistry》2022,48(4):939-954

Fish Physiology and Biochemistry - To reveal direct effects of various protein sources on digestive physiology of red seabream, Pagrus major (38.5 ± 0.4&nbsp;g), six different...  相似文献