全文获取类型
| 收费全文 | 1620篇 |
| 免费 | 59篇 |
| 国内免费 | 4篇 |
专业分类
| 林业 | 153篇 |
| 农学 | 46篇 |
| 基础科学 | 3篇 |
| 268篇 | |
| 综合类 | 62篇 |
| 农作物 | 81篇 |
| 水产渔业 | 172篇 |
| 畜牧兽医 | 766篇 |
| 园艺 | 18篇 |
| 植物保护 | 114篇 |
出版年
| 2022年 | 15篇 |
| 2021年 | 44篇 |
| 2020年 | 15篇 |
| 2019年 | 24篇 |
| 2018年 | 24篇 |
| 2017年 | 38篇 |
| 2016年 | 52篇 |
| 2015年 | 68篇 |
| 2014年 | 83篇 |
| 2013年 | 123篇 |
| 2012年 | 91篇 |
| 2011年 | 122篇 |
| 2010年 | 49篇 |
| 2009年 | 57篇 |
| 2008年 | 113篇 |
| 2007年 | 111篇 |
| 2006年 | 112篇 |
| 2005年 | 104篇 |
| 2004年 | 112篇 |
| 2003年 | 94篇 |
| 2002年 | 70篇 |
| 2001年 | 47篇 |
| 2000年 | 40篇 |
| 1999年 | 17篇 |
| 1998年 | 11篇 |
| 1997年 | 8篇 |
| 1996年 | 2篇 |
| 1995年 | 5篇 |
| 1994年 | 5篇 |
| 1993年 | 1篇 |
| 1992年 | 3篇 |
| 1991年 | 3篇 |
| 1990年 | 1篇 |
| 1989年 | 4篇 |
| 1988年 | 2篇 |
| 1987年 | 1篇 |
| 1986年 | 1篇 |
| 1984年 | 3篇 |
| 1983年 | 1篇 |
| 1982年 | 1篇 |
| 1981年 | 1篇 |
| 1977年 | 1篇 |
| 1974年 | 2篇 |
| 1973年 | 1篇 |
| 1966年 | 1篇 |
排序方式： 共有1683条查询结果，搜索用时 31 毫秒
91.
Taguchi H Watanabe S Hirao T Akiyama H Sakai S Watanabe T Matsuda R Urisu A Maitani T 《Journal of agricultural and food chemistry》2007,55(5):1649-1655
Kiwifruit (Actinidia deliciosa and Actinidia chinensis) is allergenic to sensitive patients, and, under Japanese regulations, it is one of the food items that are recommended to be declared on food labeling as much as possible. To develop PCR-based methods for the detection of trace amounts of kiwifruit in foods, two primer pairs targeting the ITS-1 region of the Actinidia spp. were designed using PCR simulation software. On the basis of the known distribution of a major kiwifruit allergen (actinidin) within the Actinidia spp., as well as of reports on clinical and immunological cross-reactivities, one of the primer pairs was designed to detect all Actinidia spp. and the other to detect commercially grown Actinidia spp. (i.e., kiwifruit, Actinidia arguta, and their interspecific hybrids) except for Actinidia polygama. The specificity of the developed methods using the designed primer pairs was verified by performing PCR experiments on 8 Actinidia spp. and 26 other plants including fruits. The methods were considered to be specific enough to yield target-size products only from the target Actinidia spp. and to detect no target-size products from nontarget species. The methods were sensitive enough to detect 5-50 fg of Actinidia spp. DNA spiked in 50 ng of salmon testis DNA used as a carrier (1-10 ppm of kiwifruit DNA) and 1700 ppm (w/w) of fresh kiwifruit puree spiked in a commercial plain yogurt (corresponding to ca. 10 ppm of kiwifruit protein). These methods would be expected to be useful in the detection of hidden kiwifruit and its related species in processed foods. 相似文献
92.
Hoshi H Yagi Y Matsunaga K Ishihara Y Yasuhara T 《Journal of agricultural and food chemistry》2007,55(21):8508-8515
We previously isolated a novel immunomodulatory alpha-(1,4)(1,6)(1,2)- d-glucan-protein complex (MPG-1) from mycelia of Tricholoma matsutake in basidiomycetes. In the present study, we raised a polyclonal antibody by immunizing rabbits with MPG-1 and constructed a sandwich enzyme-linked immunosorbent assay (ELISA) system to examine the distribution of MPG-1 among edible mushrooms and related processed foods. The system detected MPG-1 quantitatively at concentrations of more than 10 ng/mL, with a coefficient of variation of less than 10% by intra-assay and interassay precision. Analysis with the system of chemically modified MPG-1 suggested that the sugar moiety was mainly involved in the detection. The system detected MPG-1 in the extracts of the fruiting bodies of T. matsutake but not in those of 34 other basidiomycete species. Moreover, a significant amount of MPG-1 was detected in the extracts of their cultured mycelia. The antigenic structure of MPG-1 was relatively stable in terms of pH and temperature. MPG-1 was detected in processed foods supplemented with T. matsutake. These results suggest that MPG-1 is distributed predominantly in T. matsutake species and that the ELISA system can detect it in processed foods supplemented with T. matsutake. 相似文献
93.
Arnil C. Emata Hiroshi Y. Ogata Esteban S. Garibay Hirofumi Furuita 《Fish physiology and biochemistry》2003,28(1-4):489-491
Mangrove red snapper fed advanced broodstock diets containing squid meal and squid oil exhibited higher hatching rates, cumulative survival and survival activity index than those fed a basal diet or a basal diet supplemented with mixture of antioxidants. On the other hand, fatty acid analyses of ovaries and fry of wild fish and eggs and larvae of broodstock fed raw fish revealed high arachidonic acid (ARA) and docosahexaenoic acid (DHA) levels and relatively lower eicosapentaenoic acid (EPA) levels consequently showing high ARA/EPA and DHA/EPA ratios compared to cold water species. This suggests that ARA may be nutritionally more important for egg and larval development and survival in tropical marine fish and its supplementation in broodstock diets may enhance reproductive performance of mangrove red snapper. 相似文献
94.
The duality of teleost gonadotropins 总被引：5，自引：0，他引：5
Hiroshi Kawauchi Kunimasa Suzuki Hiromichi Itoh Penny Swanson Nobuko Naito Yoshitaka Nagahama Masumi Nozaki Yasumitu Nakai Seiga Itoh 《Fish physiology and biochemistry》1989,7(1-6):29-38
The duality of salmon gonadotropins has been proved by biochemical, biological, and immunological characterization of two
chemically distinc gonadotropins. GTH I and GTH II were equipotent in stimulating estradiol production, whereas GTH II appears
to be more potent in stimulating maturational steroid synthesis. The ratio of plasma levels and pituitary contents of GTHs
and the secretory control by a GnRH suggest that GTH I is the predominant GTH during vitellogenesis and early stages of spermatogenesis
in salmonids, whereas GTH II is predominant at the time of spermiation and ovulation. GTH I and GTH II are found in distinctly
separate cells. In trout, GTH I is expressed first in ontogeny, whereas GTH II cells appear coincident with the onset of spermatogenesis
and vitellogenesis, and increase dramatically at the time of final reproductive maturation. Comparison of the amino acid sequences
of polypeptides and the base sequences of cDNA revealed that salmon GTH I β is more similar to bovine FSHβ than bovine LHβ
and salmon GTH II β shows higher homology to bovine LHβ than to bovine FSHβ. The existence of two pituitary gonadotropins
in teleosts as well as tetrapods suggests that the divergence of the GTH gene took place earlier than the time of divergence
of teleosts from the main line of evolution leading to tetrapods. 相似文献
95.
96.
97.
Azusa SOMEYA Ryoko FUKUSHIMA Michiko YOSHIDA Yasuyuki TANAHASHI Tangmunkhong PRAPEUK Reiko IIZUKA Hiroshi HIRAMI Atsushi MATSUDA Shunichi TAKAHASHI Goro KURITA Takashi KIMURA Misuzu SEO Masayuki FUNABA Yoshii NISHINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1157-1160
98.
99.