Antimicrobial resistance in Escherichia coli isolated from brown rats and house shrews in markets,Bogor, Indonesia     

Hoang Le HUY  Nobuo KOIZUMI  Harimurti NURADJI  Susanti  Susan M. NOOR  NLP Indi DHARMAYANTI  Takeshi HAGA  Kazuhiro HIRAYAMA  Kozue MIURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):531

The prevalence of antimicrobial resistance (AMR) in small mammals dwelling in the city was used as an indicator of AMR bacteria in the environment. We captured 87 small mammals (79 brown rats and 8 house shrews) in four markets, Bogor, Indonesia in October 2019, and we obtained 20 AMR Escherichia coli (E. coil) from 18 brown rats and two house shrews. Of these, eight isolates were determined to be multi-drug resistant (MDR) E. coli, suggesting the potential contamination of AMR E. coli in the markets in Bogor, Indonesia, and that mammals, including humans, are at risk of infection with AMR E. coli from environment.  相似文献   

Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens     

Yen Thi Hoang NGUYEN  Nariaki NONAKA  Haruhiko MARUYAMA  Ayako YOSHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):671

This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.  相似文献   

Additional notes to the checklist of Korean cultivated plants (5). Consolidated summary and indexes     

Hoang Ho-Dzun  H. Kn¨pffer  K. Hammer 《Genetic Resources and Crop Evolution》1997,44(4):349-391

Joint explorations have been carried out in North Korea for studying and collecting cultivated plants since 1985. A checklist of the cultivated plant species of the whole Korean peninsula was developed, used and updated since the beginning of the investigations. A last additional updating is provided including new entries, a summary and indexes (families, synonyms, vernacular names). The total number of taxa considered in the checklist, including infraspecific ones, is 608. On the species level it contains 581 entries belonging to 111 families and 381 genera. The number of medicinal plants is particularly high. The data are being maintained in a database. The results demonstrate the wealth of the Korean cultivated flora which is the result of a long history and a specific Korean input.  相似文献   

Additional notes to the check-list of Korean cultivated plants (4)     

Karl Hammer  Ri Jong-Dzae  Hoang Ho-Dzun 《Genetic Resources and Crop Evolution》1990,38(3):173-190

Summary The main results of a germplasm collecting mission in the Democratic People's Republic of Korea in 1989 are presented. This exploration work and additional literature studies resulted in new information for the check-list of Korean cultivated plants (Baik et al. 1986). 61 new species could be included. For 12 species collections and/or observations are reported for the first time.

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Ergänzende Notizen zur Liste der koreanischen Kulturpflanzen (4)

Zusammenfassung Die wesentlichen Ergebnisse einer Reise zur Sammlung pflanzlicher genetischer Ressourcen 1989 in der Koreanischen Demokratischen Volksrepublik werden dargelegt. Diese Exploration und ergänzende Literaturstudien führten zu neuen Informationen für die Liste der koreanischen Kulturpflanzen (Baik et al. 1986). 61 Arten wurden neu aufgenommen. Für 12 Arten wird erstmalig über Sammlungen und/oder Beobachtungen berichtet.

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(4)

1989 - . (Baik et al. 1986). 61 . 12 / .

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红松烂皮病病原鉴定及致病性研究   总被引：3，自引：0，他引：3  

魏作全  黎明 《沈阳农业大学学报》1990,21(2):115-118

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Isolation and identification of antiplatelet aggregatory principles from the leaves of Piper lolot     

Li CY  Tsai WJ  Damu AG  Lee EJ  Wu TS  Dung NX  Thang TD  Thanh L 《Journal of agricultural and food chemistry》2007,55(23):9436-9442

The methanolic extract of Piper lolot, having shown potent inhibitory activity on platelet aggregation induced by arachidonic acid (AA) and platelet activating factor (PAF), was subjected to activity-guided isolation to yield twelve new amide alkaloids, piperlotine A-L (1-12), along with twenty-nine known compounds. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were tested for their inhibitory activity on the rabbit platelet aggregation. The compounds piperlotine A (1), piperlotine C (3), piperlotine D (4), piperlotine E (5), 3-phenyl-1-(2,4,6-trihydroxyphenyl)propan-1-one (21), 3-(4-methoxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one (22), 1-trans-cinnamoylpyrrolidine (24), sarmentine (26), pellitorine (27), methyl 3-phenylpropionate (32), and (10S)-10-hydroxypheophorbide a methyl ester (40) showed potent antiplatelet aggregation activity.  相似文献   

Properties and activities of blood‐ or seawater‐contaminated wild‐caught Striped Jewfish (Stereolepis doederleini) sperm          下载免费PDF全文

Minh Hoang Le  Young Jin Chang  Augustine Arukwe 《Aquaculture Research》2018,49(2):900-907

Understanding the effects of environmental factors in sperm qualities will be helpful in the development of optimal artificial reproduction methods and contributes towards the knowledge base of better short‐ and long‐term fish semen preservation conditions The objectives of this study were to determine properties and activities of wild‐caught striped jewfish Stereolepis doederleini sperm contaminated with blood or seawater and compare them with data reported in the literature on other freshwater and marine fish species, for effective short‐ and long‐term storage of fish semen. Overall, we observed that the sodium, chloride, glucose, total protein concentrations of normal sperm were not significantly different from blood‐ or seawater‐contaminated sperm. The salinity and osmolality concentration of sperm contaminated with blood were lower than sperm contaminated with seawater and were not significantly different from normal sperm. In addition, the spermatozoa motility (SM) and duration of spermatozoa motility (DSM) in blood‐contaminated sperm were higher than seawater‐contaminated sperm and also not significantly different from normal sperm. The best condition for SM and DSM in normal sperm was dilution rate of 1:50. Sperm was immotile in distilled water, and cationic factors were shown to stimulate the initiation of spermatozoa activation. The maximum SM and DSM were observed in solution containing 0.4 M NaCl, 0.6 M KCl, 0.6 M CaCl₂ and 0.4 M MgCl₂. This study provides some basic and important knowledge about striped jewfish sperm sensitivity to a cationic condition. In this regard, Na⁺ is the major inhibitory factor of spermatozoa motility in this fish species.  相似文献   

Root-specific expression of defensin in transgenic tobacco results in enhanced resistance against <Emphasis Type="Italic">Phytophthora parasitica</Emphasis> var. <Emphasis Type="Italic">nicotianae</Emphasis>     

Hyun-Hwa Lee  Jin-Sol Kim  Quyen T. N. Hoang  Jeong-Il Kim  Young Soon Kim 《European journal of plant pathology / European Foundation for Plant Pathology》2018,151(3):811-823

Phytophthora species are soil-borne pathogens that damage plants in both agro- and natural ecosystems. To suppress the devastating pathogen, we generated a root-specific expression system using a specific promoter (pPRP3) conferring elevated expression of the target gene in roots that are very susceptible to soil-borne pathogens. To verify root-specific expression, we compared β-glucuronidase (GUS) expression driven by a constitutive or root-specific promoters in shoots and roots. In histochemical and fluorometric assays, GUS activity was detected in whole tobacco plants when GUS expression was driven by p35S, but was detected only in the roots by pPRP3. We then expressed a pepper defensin (J1–1) gene in tobacco to elucidate its effect on plant resistance. The accumulation of J1–1 was also tissue-specific in transgenic tobacco plants. Finally, transgenic plants carrying GUS or J1–1 genes in combination with p35S or pPRP3 were inoculated with Phytophthora parasitica var. nicotianae and Pythium aphanidermatum. Disease symptoms were significantly suppressed in transgenic plants that accumulated J1–1, regardless of the promoter used. Furthermore, the expression of PR genes was induced in J1–1 transgenic plants, exhibiting much higher levels in p35S-driven J1–1 plants than in pPRP3::J1–1 plants. These results demonstrated that J1–1 transgenic plants were primed for enhanced expression of PR genes, which provided synergistic effects with the defensin for disease resistance.  相似文献   

黄瓜(Cucumis sativusL.)花药培养     

陈劲枫 《中国瓜菜》2012,(2):1-5

以卡罗尔、HH1-8-57与津绿农家乐3个不同基因型黄瓜品种为试材,在Kumar等、Song等和詹艳等试验研究基础上,设计试验分别探讨低温预处理时间、培养基成分(胚性愈伤组织诱导培养基、胚状体诱导培养基)和基因型等因素对黄瓜花药培养的影响。结果表明:在4℃低温预处理2d时,胚性愈伤组织诱导率显著高于其他处理;在MS+1.0mg·L-12,4-D+0.5mg·L-16-BA+3%蔗糖+0.8%琼脂培养基上胚性愈伤组织诱导率最高,达到81.3%;在MS+0.1mg·L-1NAA+3.0mg·L-16-BA+3%蔗糖+0.8%琼脂培养基上胚状体诱导率最高,为40.0%;基因型间胚性愈伤组织诱导率及胚状体诱导率差异显著,津绿农家乐品种胚性愈伤组织诱导率最高,达到81.1%,HH1-8-57胚状体诱导率最高,为40.0%。本研究从2份试材中成功地诱导出胚状体并获得了黄瓜单倍体再生植株。  相似文献   

Influence of mulch C/N ratio and decomposition stage on plant N uptake and N availability in soil with or without wheat straw     

Thi Hoang Ha Truong  Paul Kristiansen  Petra Marschner 《植物养料与土壤学杂志》2019,182(6):879-887

Mulches can improve soil properties, but little is known about nutrient availability in mulched soil that contains plant residues and the effect of mulching with manures. The aim of this study was to determine the effects of mulching with high or low C/N organic materials, in which low C/N materials differed in decomposability, and the presence of wheat straw in the soil on plant growth and N uptake, soil N availability and microbial biomass N within about four months after mulching. Three organic materials were used: mature wheat straw (W, C/N 80), young faba bean shoots (FB, C/N 7), and sheep manure (SM, C/N 8). There were eight treatments differing in amendment methods (mulching or mixing with W or both) and mulching materials (W, FB or SM). Treatments that were only mulched with W, FB or SM are referred to as m‐treatments. In m/s‐treatments, after W was mixed into the soil, W, FB or SM were placed on the soil surface as mulch. Two other treatments included an unamended control and soil mixed with W. Wheat was planted 0, 35 or 70 days after mulching (referred to as 0, 35, and 70 DAM) and grown for 35 days. Faba bean mulch increased shoot dry weight, shoot N uptake and available N compared to wheat or sheep manure mulch, particularly in the m‐treatments. Shoot dry weight was higher in m‐treatments than corresponding m/s‐treatments with the same mulch type. Shoot N uptake was higher in 70 DAM than in 0 DAM in all treatments and 0.3 to three‐fold higher in m‐treatments than the corresponding m/s‐treatments. Microbial biomass N was higher in 0 DAM than in 35 and 70 DAM in most treatments and up to two‐fold higher in m/s‐treatments than the corresponding m‐treatments. Available N in m/s‐treatments was two to six‐fold higher than m‐treatments in 0 DAM, but differed little in older mulch ages of W and SM. It can be concluded that compared to soil with only mulch, mixing of wheat straw into soil reduced plant growth and N uptake, particularly in the early stages of mulching (0 and 35 DAM). However, the presence of wheat in mulched soil may provide a longer lasting source of N for plants and reduce the risk of N leaching from rapidly decomposing low C/N mulch due to greater microbial biomass N uptake than only soil with mulch.  相似文献