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81.
Lalueza-Fox C Römpler H Caramelli D Stäubert C Catalano G Hughes D Rohland N Pilli E Longo L Condemi S de la Rasilla M Fortea J Rosas A Stoneking M Schöneberg T Bertranpetit J Hofreiter M 《Science (New York, N.Y.)》2007,318(5855):1453-1455
The melanocortin 1 receptor (MC1R) regulates pigmentation in humans and other vertebrates. Variants of MC1R with reduced function are associated with pale skin color and red hair in humans of primarily European origin. We amplified and sequenced a fragment of the MC1R gene (mc1r) from two Neanderthal remains. Both specimens have a mutation that was not found in approximately 3700 modern humans analyzed. Functional analyses show that this variant reduces MC1R activity to a level that alters hair and/or skin pigmentation in humans. The impaired activity of this variant suggests that Neanderthals varied in pigmentation levels, potentially on the scale observed in modern humans. Our data suggest that inactive MC1R variants evolved independently in both modern humans and Neanderthals. 相似文献
82.
The splicing factor SF3b is a multiprotein complex essential for the accurate excision of introns from pre-messenger RNA. As an integral component of the U2 small nuclear ribonucleoprotein (snRNP) and the U11/U12 di-snRNP, SF3b is involved in the recognition of the pre-messenger RNA's branch site within the major and minor spliceosomes. We have determined the three-dimensional structure of the human SF3b complex by single-particle electron cryomicroscopy at a resolution of less than 10 angstroms, allowing identification of protein domains with known structural folds. The best fit of a modeled RNA-recognition motif indicates that the protein p14 is located in the central cavity of the complex. The 22 tandem helical repeats of the protein SF3b155 are located in the outer shell of the complex enclosing p14. 相似文献
83.
Investigations of 23 northwestern German sandy Ap horizons (mean clay content 35 g kg−1), that had higher organic matter (OM) levels than expected for sands, showed that the bulk soil C to N ratio reliably indicated the release of N from stabilized OM. Soils were incubated at 35 °C for 200 days under aerobic conditions. Cumulative N release curves were split into N released from fresh materials (Nfast) and N released from the larger pool of stabilized, older OM (Nslow rates, 0.06-0.77 μg N g−1 soil d−1, or 0.7-49 μg N g−1 OM). Correlating the Nslow rates with total N contents of soils yielded no satisfactory relationships while their relationship with C to N ratios was very close (negative exponential, R2=0.88). Low rates of N release (Nslow) per unit of OM occurred if C to N exceeded 15. This was associated with historical factors like podzolization, calluna heathland, plaggen fertilization or a combination of these. 相似文献
84.
Selected isoflavones (genistein, daidzein, formononetin, prunetin, biochanin A, and two synthetic isoflavones) were allowed to interact with soy and whey proteins. The reaction products were analyzed in terms of covalent binding at the nucleophilic side chains of proteins. Changes in molecular properties of the proteins derivatives were documented by SDS-PAGE, IEF, and SELDI-TOF-MS. The structural changes induced were studied using circular dichroism. The in vitro digestibility was assessed with trypsin. The results show that the occurrence of the catechol moiety, that is, the two adjacent (ortho) aromatic hydroxyl groups on ring B of the flavonoid structural skeleton appears to be prerequisite condition for covalent binding to proteins. The catechol moiety on ring A was less reactive. Its absence lead to a slight or no significant reaction, although noncovalent interactions may still be possible, even when lacking this structural element. A comparison of the data is also made with quercetin representing the flavonols. 相似文献
85.
Takashi Shimizu Tsutomu Takagi Holger Korte Tomonori Hiraishi Katsutaro Yamamoto 《Fisheries Science》2007,73(3):489-499
ABSTRACT: The net-shape and loading analysis system (NaLA) was developed to determine fishing net configuration and load in a previous study. The system has since been applied to general gill nets and aquaculture nets, and its validity has been proven through model experiments in tanks. In this study, the system was applied to estimate the dynamic behavior of a bottom gill net for walleye pollock, to test the system's applicability of the system to gear operations in the field. To obtain in situ data, four bottom gill net operations were performed in February 2004 off the coast of Sawara, Hokkaido, Japan. During operations, vertical displacements of the bottom gill net's float and sinker lines were measured as representative values of gear behavior, and ocean current direction, and speed at the gear position were observed simultaneously. Then, bottom gill net behavior was simulated using NaLA, incorporating observed environmental conditions and gear specifications. The resulting calculated behavior was compared to measured behavior in terms of the relationship between net height and environmental or setting conditions. Agreement between the calculated and measured net behavior was found. Thus, it is believe that our NaLA calculation model has the potential to simulate the dynamic behavior of bottom gill nets in situ . 相似文献
86.
The aim of this study was to use energy-dispersive X-ray spectroscopy (EDX) to localize chitosan in the cell wall of chitosan-impregnated
Scots pine. It was of interest to investigate the concentration of chitosan in wood to gain further knowledge and understanding
of the distribution of chitosan in the wooden matrix. After deacetylation, chitosan was re-acetylated with chloroacetic anhydride
to achieve a covalent bonding of chloride to the chitosan polymer. Chloride-labelled chitosan was measured by EDX using a
scanning electron microscope and described as chloride intensity. Analysis of free chloride anions was performed by dialysis
and inductively coupled plasma atomic emission spectroscopy. There was a significant correlation between the molecular weight
of chitosan and the intensity of covalent-bonded chloride to the chitosan polymer. High molecular weight chitosan showed a
better interaction with the cell wall structure than low molecular chitosan. 相似文献
87.
A vegetation study was carried out to investigate the carryover of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) from soil mixed with contaminated sewage sludge to potato, carrot, and cucumber plants. Analysis was done by liquid-extraction using acetonitrile with dispersive SPE cleanup and subsequent HPLC-MS/MS. In order to assess the transfer potential from soil, transfer factors (TF) were calculated for the different plant compartments: TF = [PFC](plant (wet substance))/[PFC](soil (dry weight)). The highest TF were found for the vegetative plant compartments with average values for PFOS below those for PFOA: cucumber, 0.17 (PFOS), 0.88 (PFOA); potato, 0.36 (PFOS), 0.40 (PFOA); carrot, 0.38 (PFOS), 0.53 (PFOA). Transfer of PFOA and PFOS into potato peelings (average values of TF: PFOA 0.03, PFOS 0.04) exceeded the carryover to the peeled tubers (PFOA 0.01, PFOS < 0.01). In carrots, this difference did not occur (average values of TF: PFOA 0.04, PFOS 0.04). Transfer of PFOS into the unpeeled cucumbers was low and comparable to that of peeled potatoes (TF < 0.01). For PFOA, it was higher (TF: 0.03). 相似文献
88.
PRACTICAL RELEVANCE: Weakness is a relatively common clinical presentation in feline medicine and can be caused by primary neuromuscular disease or by diseases of other body systems affecting the neuromuscular system secondarily. Successful work-up relies on a thorough clinical and neurological examination, and logical problem solving, based on an understanding of the underlying neuroanatomical and pathophysiological mechanisms. CLINICAL CHALLENGES: Feline neuromuscular diseases can be a diagnostic challenge. On initial inspection, the presenting signs can mimic disorders of other body systems, particularly cardiovascular, pulmonary and orthopaedic disease, or may be confused with systemic illnesses. Additionally, because many different pathologies of the feline neuromuscular system converge to a similar clinical phenotype, further diagnostic steps such as electrodiagnostics, cerebrospinal fluid analysis, and muscle and nerve biopsies must be considered even after neuromuscular dysfunction has been identified. AUDIENCE: This review provides a framework for the clinical approach to the weak cat and gives a practical summary of neuromuscular diseases for the general practitioner and specialist alike. EVIDENCE BASE: Many diseases affecting the feline neuromuscular system have been well described in the veterinary literature, mostly based on retrospective case reports and series. The evidence base for the treatment of feline neuromuscular diseases remains very limited. 相似文献
89.
Cellular defense of the avian respiratory system. Influx of phagocytes: elicitation versus activation 总被引:2,自引:0,他引:2
We studied various means of inducing avian phagocytes to migrate to the respiratory tract. No significant and consistent increases in the number of avian respiratory phagocytes (ARP) were elicited by intravenous inoculation with Escherichia coli lipopolysaccharide (LPS), Saccharomyces cerevisiae glucan (G), and Freund's incomplete adjuvant (FIA) in a water-in-oil-in-water emulsion; subcutaneous inoculation with the LPS-G-FIA homogenate; or aerosolized exposure to LPS-G-FIA, thioglycolate, and proteose-peptone. Intravenous inoculation with heat-killed Corynebacterium parvum resulted in a significant increase in the number of ARP by day 6 after inoculation; intratracheal inoculation of C. parvum effected a more rapid and higher level of phagocyte migration to the respiratory tract. Intratracheally administered E. coli induced significant migration of phagocytes to the respiratory system so that by 24 hours postinoculation, the group average number of ARP was about 50-100 times as high as the number in unstimulated control birds. None of the birds yielding high numbers of phagocytes from their respiratory tract had signs of respiratory disease. 相似文献
90.
青藏高寒草甸部分豆科植物根瘤菌的初步研究 总被引:1,自引:0,他引:1
本文首次对青藏高原川西北高寒草地部分豆科植物进行了根瘤菌共生调查和性质研究,在若尔盖、红原、松潘等地海拔在3000-3500m的草地上采集豆科植物根瘤样品50多份,分别与24种不同的豆科植物共生结瘤,从中分离到21株根瘤菌,研究发现,高原高寒盖草地豆科植物种类不如低海拔地区多,根瘤亦不发达,普遍偏小,且无效根瘤较多。由于气温度,根瘤菌对根的感染力不够强,但根瘤菌对低温和紫外线等不利因素有较强的抵抗 相似文献