Amplified fragment length polymorphism as a tool for molecular characterization of almond germplasm: genetic diversity among cultivated genotypes and related wild species of almond,and its relationships with agronomic traits     

Karim Sorkheh  Behrouz Shiran  T. M. Gradziel  B. K. Epperson  Pedro Martínez-Gómez  E. Asadi 《Euphytica》2007,156(3):327-344

Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient method for producing DNA fingerprints and molecular characterization. Our objectives were to: estimate genetic similarities (GS), marker indices, and polymorphic information contents (PICs) for AFLP markers in almond cultivars; assess the genetic diversity of almond cultivars and wild species, using GS estimated from AFLP fingerprints and molecular characterization; and facilitate the use of markers in inter-specific introgression and cultivar improvement. The genetic diversity of 45 almond cultivars from Iran, Europe, and America, were studied assaying 19 primer combinations. In addition, several agronomic traits were evaluated, including flowering and maturity times, self-incompatibility, and kernel and fruit properties. Out of the 813 polymerase chain reaction fragments that were scored, 781 (96.23%) were polymorphic. GS ranged from 0.5 to 0.96, marker indices ranged from 51.37 to 78.79, and PICs ranged from 0.56 to 0.86. Results allowed the unique molecular identification of all assayed genotypes. However, the correlation between genetic similarity clustering as based on AFLP and clustering for agronomic traits was low. Cluster analysis based on AFLP data clearly differentiated the genotypes and wild species according to their origin and pedigree, whereas, cluster analysis based on agronomic data differentiated according the pomological characterization. Our results showed the great genetic diversity of the almond cultivars and their interest for almond breeding.  相似文献   

Effects of high- vs. low-yield environments on selection for increased biomass yield in switchgrass     

Louis W. RoseIV  Modan K. Das  Roger G. Fuentes  Charles M. Taliaferro 《Euphytica》2007,156(3):407-415

No information is available on the effects of different biomass yield environments on selection efficiency in switchgrass (Panicum virgatum L.) breeding improvement. This study was conducted to assess the effects of high- and low-biomass yield environments (HYE and LYE, respectively) on recurrent selection for general combining ability (RSGCA) in a lowland population of switchgrass (NL-94). The top 14 of 65 NL-94 C₀ parent plants were selected based on biomass yield of half-sib (HS) progeny tested for one post-establishment year under HYE and LYE conditions. Nine of the 14 C₀ parent plants were the same based on HS performance under HYE and LYE. Selected plants were intercrossed to produce NL-94 HYE and NL-94 LYE C₁ populations. One hundred and twenty-five HS C₁ progeny families (60 NL-94 HYE and 65 NL-94 LYE) were evaluated for biomass yield for 3 years (2002–2004) under HYE and LYE conditions. The HYE produced about 2.5 times higher biomass yields than the LYE in both C₀ and C₁ HS progeny tests. Estimated additive genetic variance and predicted gains from selection (ΔG) were high in the C₁ populations indicating that RSGCA should achieve higher biomass yields. Mean biomass yields of C₁ HS families originating from the LYE protocol were significantly higher than those of families originating from the HYE protocol in both HYE and LYE performance tests, suggesting greater selection response under LYE in the C₀ population. The estimates of narrow-sense heritability ( ) and ΔG from the C₁ populations indicate that positive response to selection for biomass yield is possible in subsequent cycles of selection under either HYE or LYE, with a possible small advantage for HYE.  相似文献   

Tartary buckwheat breeding (<Emphasis Type="Italic">Fagopyrum tataricum</Emphasis> L. Gaertn.) through hybridization with its Rice-Tartary type     

Yingjie Wang  Clayton G. Campbell 《Euphytica》2007,156(3):399-405

Tartary buckwheat (Fagopyrum tataricum) is a highly nutritious crop that is widely grown in Asia, but the flour contains a large portion of the hull if it is ground with industrial processing since the hull is very hard to remove as it adheres to the testa layer of the groat. Rice-Tartary, a particular type of Tartary buckwheat, has seeds with a loose hull and the presence of splits on the sides of the seeds that make dehulling easily. The present study reports on the first attempt of crop improvement in Tartary buckwheat breeding through hybridization. Hybrids were obtained by hybridization of crosses between Tartary buckwheat and Rice-Tartary. Additional crosses were made among selected progenies of these crosses. Based on progeny analysis of the F₁, F₂, and F₃, the character of Rice-Tartary, as exhibited as the presence of splits on the sides of the seeds, is controlled by one gene which is homozygous recessive. A Tartary buckwheat breeding program has been conducted for 6 years based on these crosses. Advanced lines with easy dehull and yield potential are at the stage of ready for yield trials. Production of easy dehulling Tartary buckwheat in the future could boost Canada’s domestic and international markets.  相似文献   

Regional phenotypic diversity of Puccinia triticina and wheat host resistance in western Europe, 1995     

Robert F. Park  Henriette Goyeau  Friedrich G. Felsenstein  Pavel Bartoš  Friedrich J. Zeller 《Euphytica》2001,122(1):113-127

Pathogenicity data from surveys of Puccinia triticina (formerly P. recondita f. sp. tritici) conducted in western Europe in 1995 were analysed to compare the structure of regional populations of the pathogen. Many of the populations differed in phenotypic diversity and pathotypic composition, even though they occurred within a single epidemiological unit, suggesting that local factors may influence the establishment and propagation of individual pathotypes in the regional populations. Neighbouring regions were more similar than distant regions, and all regions shared at least one pathotype, except populations in northern Italy and Scotland. A high degree of similarity was found between populations in northern France and Great Britain, providing strong evidence of free movement of inoculum between these regions. Resistance genes were postulated for a selection of 91 wheat cultivars, representing those most commonly grown in western Europe in 1995. Thirteen cultivars lacked detectable seedling resistance genes and the remaining 78 possessed from one to three resistance genes; those detected were Lr1, Lr3a, Lr10, Lr13, Lr14a, Lr17b, Lr20, Lr26 and Lr37. The most commonly detected resistance gene was Lr13, which was present singly or in combination with other resistance genes in 48 cultivars (53%). The gene Lr14a was detected in 18 cultivars, Lr26 was present in 16 cultivars. The role of host selection in the composition of the regional populations of P. triticina in western Europe in 1995 was difficult to assess on the basis of the results obtained, since virulence data were not available for Lr13 and Lr14a. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Hybridizing <Emphasis Type="Italic">Brassica rapa</Emphasis> with wild crucifers <Emphasis Type="Italic">Diplotaxis erucoides</Emphasis> and <Emphasis Type="Italic">Brassica maurorum</Emphasis>     

Harsh Garg  Shashi Banga  Payal Bansal  Chhaya Atri  S. S. Banga 《Euphytica》2007,156(3):417-424

Two wide hybrids, Diplotaxis erucoides (2n = 14) × Brassica rapa (2n = 20) and B. maurorum (2n = 20) × B. rapa, were developed using the sequential ovary–ovule culture. Reciprocal crosses failed, possibly as a consequence of strong unilateral incompatibility. The F ₁ hybrids in each combination were completely male sterile and morphologically intermediate to the respective parents. DNA marker polymorphism and chromosome counts confirmed their hybrid nature. High frequency of bivalents in the F ₁ and the presence of trivalents/quadrivalents in the derived amphiploids suggested genomic duplications and homoeology of the parental genomes. Up to three homoeologous pairs between the D. erucoides (D^eD^e) and B. rapa (AA) genomes, and one between B. maurorum (B^mB^m) and B. rapa genomes were observed. Successful synthesis of the F ₁ hybrids and amphiploids of B. rapa with D. erucoides and B. maurorum, and allosyndetic chromosome pairing are expected to permit introgressions of desirable loci into the cultivated Brassica germplasm, especially for resistance to Alternaria brassicae and Albugo candida.  相似文献   

低温对杂交一代黄瓜幼苗生理特性的影响   总被引：18，自引：1，他引：18  

沈文云  侯锋 《华北农学报》1995,10(1):56-59

对四个黄瓜亲本及其杂交一代（Ｆ１）的生理特性试验研究表明，Ｆ１的相对电导值与双亲的相对电导值呈正相关关系。ＭＤＡ含量在低温处理４８ｈ后上升，７２ｈ后下降，较耐低温亲本的Ｆ１的ＭＤＡ含量低于双亲平均值，不耐低温亲本的Ｆ１的ＭＤＡ含量高于双亲的平均值。２低温处理７２ｈ后ＣＡＴ酶活性下降。ＰＯＤ酶活性上升；ＰＯＤ酶同工酶分析表明，低温处理后酶带数增加，耐低温的Ｆ１酶带增加较多。  相似文献   

Inheritance and relationships of important agronomic traits in almond   总被引：2，自引：0，他引：2  

Raquel Sánchez-Pérez  Encarnación Ortega  Henri Duval  Pedro Martínez-Gómez  Federico Dicenta 《Euphytica》2007,155(3):381-391

The inheritance of 16 important agronomic traits and its relationship were studied for four years in a population of 167 almond [P. dulcis (Mill.) D.A. Webb] seedlings obtained from a cross between the French selection ‘R1000’ (‘Tardy Nonpareil’ × ‘Tuono’) and the Spanish cultivar ‘Desmayo Largueta’. For some traits (blooming and leafing date) descendants segregated between the value of both progenitors, meanwhile for others the mean of the offspring was lower (bloom density, productivity and ripening date) or higher (in-shell/kernel ratio and double kernels). As expected, kernel bitterness and self-incompatibility behaved as monogenic traits. Some important correlations between traits were detected. The implications of the transmission and the correlation of these traits in the breeding programmes are discussed.  相似文献   

代料不同配方培养基对木耳菌丝体生长影响的研究   总被引：1，自引：0，他引：1  

陈文良  陈卓雄 《华北农学报》1991,6(1):115-120

通过代料不同配方培养基对木耳菌丝体生长影响的研究,明确配方1培养基上木耳菌丝体生长较快,菌丝体浓白、稠密、生长茁壮,栽培袋污染率低.这是由于配方1的营养、碳氮比和物理性状适于木耳菌丝体生长的缘故.配方1的成份为:棉子皮48%,锯末48%,石膏1%,磷肥1%,石灰1.5%,尿素0.5%.  相似文献   

THE ORGANIZED DEVELOPMENT TREND OF CEMENT CONCRETE     

Yan Wunan Hou Haolin 《保鲜与加工》1989,(4)

Cement concrete is the building material which is widely and lar-gely used.Congenital deficiency of cement concrete is brittleness.Adding a little polymer into cement concrete can improve its brittleness and function and even provide concrete with new function.This is an organized development trend of cement concrete.  相似文献   

Induction and development of microspore-derived embryos in broccoli × white-headed cabbage hybrids microspore culture     

Aisong Zeng  Jiyong Yan  Lixiao Song  Bing Gao  Yunxia Zhang  Jianqi Li  Huanhuan Liu  Xilin Hou  Ying Li 《Euphytica》2015,203(2):261-272

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