Quantitative bioluminometric method for DNA-based species/varietal identification in food authenticity assessment     

Trantakis IA  Christopoulos TK  Spaniolas S  Kalaitzis P  Ioannou PC  Tucker GA 《Journal of agricultural and food chemistry》2012,60(4):912-916

A method is reported for species quantification by exploiting single-nucleotide polymorphisms (SNPs). These single-base changes in DNA are particularly useful because they enable discrimination of closely related species and/or varieties. As a model, quantitative authentication studies were performed on coffee. These involved the determination of the percentage of Arabica and Robusta species based on a SNP in the chloroplastic trnL(UAA)-trnF(GAA) intraspacer region. Following polymerase chain reaction (PCR), the Robusta-specific and Arabica-specific fragments were subjected to 15 min extension reactions by DNA polymerase using species-specific primers carrying oligo(dA) tags. Biotin was incorporated into the extended strands. The products were captured in streptavidin-coated microtiter wells and quantified by using oligo(dT)-conjugated photoprotein aequorin. Aequorin was measured within 3 s via its characteristic flash-type bioluminescent reaction that was triggered by the addition of Ca(2+). Because of the close resemblance between the two DNA fragments, during PCR one species serves as an internal standard for the other. The percentage of the total luminescence signal obtained from a certain species was linearly related to the percent content of the sample with respect to this species. The method is accurate and reproducible. The microtiter well-based assay configuration allows high sample throughput and facilitates greatly the automation.  相似文献   

Bioactive Lipids of Marine Microalga Chlorococcum sp. SABC 012504 with Anti-Inflammatory and Anti-Thrombotic Activities     

Katie Shiels  Alexandros Tsoupras  Ronan Lordan  Constantina Nasopoulou  Ioannis Zabetakis  Patrick Murray  Sushanta Kumar Saha 《Marine drugs》2021,19(1)

Microalgae are at the start of the food chain, and many are known producers of a significant amount of lipids with essential fatty acids. However, the bioactivity of microalgal lipids for anti-inflammatory and antithrombotic activities have rarely been investigated. Therefore, for a sustainable source of the above bioactive lipids, the present study was undertaken. The total lipids of microalga Chlorococcum sp., isolated from the Irish coast, were fractionated into neutral-, glyco-, and phospho-lipids, and were tested in vitro for their anti-inflammatory and antithrombotic activities. All tested lipid fractions showed strong anti-platelet-activating factor (PAF) and antithrombin activities in human platelets (half maximal inhibitory concentration (IC₅₀) values ranging ~25–200 μg of lipid) with the highest activities in glyco- and phospho-lipid fractions. The structural analysis of the bioactive lipid fraction-2 revealed the presence of specific sulfoquinovosyl diacylglycerols (SQDG) bioactive molecules and the HexCer-t36:2 (t18:1/18:1 and 18:2/18:0) cerebrosides with a phytosphingosine (4-hydrosphinganine) base, while fraction-3 contained bioactive phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules. These novel bioactive lipids of Chlorococcum sp. with putative health benefits may indicate that marine microalgae can be a sustainable alternative source for bioactive lipids production for food supplements and nutraceutical applications. However, further studies are required towards the commercial technology pathways development and biosafety analysis for the use of the microalga.  相似文献   

Towards sweet cherry (<Emphasis Type="Italic">Prunus avium</Emphasis> L.) breeding: phenotyping evaluation of newly developed hybrids     

Ioannis Ganopoulos  Anna-Maria Farsakoglou  Filippos Aravanopoulos  Athanassios Molassiotis  Michail Michailidis  Ermioni Malliarou  Evangelia Avramidou  Athanasios Tsaftaris  Maslin Osanthanunkul  Panagiotis Madesis  Konstantinos Kazantzis  Aliki Xanthopoulou 《Euphytica》2018,214(6):99

An increasing demand for cherry production (Prunus avium L.) in Greece led to the development of new high quality sweet cherry cultivars. Self-incompatibility in cherry is one of the most challenging issues for the species’ cultivation and top breeding priority. Τhe present study focuses on the development of new hybrids with improved traits such as productivity, fruit size, organoleptic characteristics and self-compatibility. For this purpose, thirty different cultivars were crossed and produced hybrids that were evaluated according to 34 morpho-physiological characteristics. The results were analyzed using the XLSTAT (version 2014.1) software and a dendrogram was constructed using the agglomerative hierarchical clustering method. Optimal hybrid clustering was achieved when characteristics of great economic importance such as fruit shape and size, growth habit and days to blooming were included in the analysis. Based on the results, new sweet cherry hybrids with the special character of self-compatibility were developed. Our findings provide crucial new information for sweet cherry future breeding programs and cultivation.  相似文献   

First report of canine systemic cryptococcosis owing to Cryptococcus gattii in Europe          下载免费PDF全文

Spyridoula Gerontiti  Ioannis L. Oikonomidis  Lambrini Kalogianni  Nektarios Soubasis  Maria Kritsepi‐Konstantinou  Aristea Velegraki  Anastasia Komnenou  Eleptherios Triantafyllou 《The Journal of small animal practice》2017,58(1):58-59

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Barrage formation is independent from heterokaryon incompatibility in Verticillium dahliae     

Ioannis A. Papaioannou  Milton A. Typas 《European journal of plant pathology / European Foundation for Plant Pathology》2015,141(1):71-82

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Competitive interactions of fifty barley cultivars with Avena sterilis and Asperugo procumbens     

Kico Dhima  Ioannis Vasilakoglou  Thomas Gatsis  Ilias Eleftherohorinos 《Field Crops Research》2010

A 2-year study was conducted in northern Greece to assess the ability of 29 six-row and 21 two-row barley (Hordeum vulgare L.) cultivars to compete with winter wild oat [Avena sterilis spp. ludoviciana (Durieu) Gill & Magne] and German-madwort (Asperugo procumbens L.). The fresh weight of both weeds grown in competition with six of the most competitive six-row barley cultivars (Bulk Resist. Desc-Sutter, Lignee 640, Paico's, Mantnan's-EH 165, Pistacho and Malebo) was reduced by 61–75% compared with one of the least competitive six-row cultivars [Gloria's-Copal's (30B-4Y-20M)]. The corresponding fresh weight reduction of both weeds grown in competition with three of the most competitive two-row barley cultivars (Goldmarker, Shanon and Helena) ranged from 59 to 76% compared with one of the least competitive two-row cultivars (Guanajuato). The ability to withstand competition (AWC) from winter wild oat and German-madwort of the six-row and two-row cultivars ranged from 33.7 to 78.3% and 26.7 to 69.2%, respectively. Representative yield losses ranged from 24 to 68% and 30 to 75%, respectively. The corresponding ability to compete (AC) indices ranged from 22.5 to 64.8% and 21.1 to 59.1%. The results of this study indicated that the barley cultivars Ligne 640 and Pistacho (2B-1Y-1B) had high AWC from winter wild oat and German-madwort and provided high grain yield in both weedy and weed-free conditions. Consequently, they could successfully be used in Mediterranean organic (no herbicide use) and conventional agricultural systems with reduced herbicide use. Barley cultivars with high AC and ability to produce high grain yield under weedy conditions [with Bulk Resist. Desc-Sutter, Arupo's and Gloria's-Copal's (30B-4Y-23M) among the most efficient] could also successfully be used in organic fields infected by the two weeds.  相似文献   

Nutritional quality of greenhouse lettuce at harvest and after storage in relation to N application and cultivation season     

Eleni Konstantopoulou  Georgios Kapotis  Georgios Salachas  Spyridon A. Petropoulos  Ioannis C. Karapanos  Harold C. Passam 《Scientia Horticulturae》2010

The effect of five levels of nitrogen fertilization on the growth and nutritional quality of Cos lettuce (Lactuca sativa L. cv. Parris Island) at harvest and after storage was studied during autumn and winter in South-West Greece. Plants were cultivated hydroponically in a greenhouse and the nitrate, chlorophyll and ascorbic acid (vitamin C) concentrations within the plant tissues were measured at harvest and following storage at 5 or 10 °C for 10 days. Nitrate accumulated in the leaves with increasing amounts of N within the nutrient solution and was higher in the winter than in the autumn. At the lowest N level (20 mg L⁻¹), the inner leaves accumulated more nitrate than the outer leaves, whereas at higher N levels (140, 200 or 260 mg L⁻¹) nitrate accumulation was higher in the outer leaves. Overall, the highest nitrate concentrations were detected in the petiole and the proximal end of the leaf, but at the lowest N application rate (20 mg L⁻¹) nitrate accumulated in the distal region of the leaf too. Although the nitrate concentrations within the leaves did not change significantly during 10 days storage at 5 or 10 °C, the chlorophyll and vitamin C concentrations decreased. Chlorophyll loss was higher in lettuce that was grown under low N levels and was higher at 10 °C than at 5 °C, but was reduced by enclosure of the lettuce in polyethylene film. It is concluded that the optimum N application rate for Cos lettuce grown hydroponically under cover during autumn and winter in South-West Greece, and in other areas with a similar climate, is 200 mg N L⁻¹ because at this N rate yield is satisfactory and leaf nitrate concentrations are below the maximum acceptable level for human consumption. Nutritional value (vitamin C concentration) and market quality (chlorophyll content) are highest at harvest and decrease during storage, but quality in terms of nitrate concentration does not change.  相似文献   

Evaluation of zinc supplementation in European sea bass (Dicentrarchus labrax) juvenile diets     

Eleni Fountoulaki  Henry Morgane  George Rigos  Vasilaki Antigoni  Eleni Mente  John Sweetman  Ioannis Nengas 《Aquaculture Research》2010,41(9):e208-e216

This study aimed to investigate the effect of increased zinc dietary levels on the growth performance, feed utilization, immune status and induced wound healing in European sea bass (Dicentrarchus labrax). Fish weighing 10 g were fed five diets containing organic zinc at 30, 70, 110 and 150 mg kg^?1 diet and one inorganic zinc source (zinc oxide) at 150 mg kg^?1 diet for a period of 12 weeks. No significant (P>0.05) differences were found in the growth performance parameters (final weight, specific growth rate), immunological indices tested (respiratory burst activity in whole blood) and the wound‐healing process. Zinc accumulation in the skin, vertebrae and liver increased significantly (P<0.01) with increased dietary zinc levels but not in muscle. The highest zinc concentrations were obtained in skin tissue, followed by vertebrae, liver and muscle tissue. Using the concentration of zinc in skin as a response criterion, broken‐line analysis showed that the supplementation of 148 mg organic Zn kg^?1 diet seemed to be the optimum dietary zinc supplementation level for sea bass juveniles.  相似文献   

Local management and landscape drivers of pollination and biological control services in a Kenyan agro-ecosystem     

Mark Otieno  Ben A. Woodcock  Andrew Wilby  Ioannis N. Vogiatzakis  Alice L. Mauchline  Mary W. Gikungu  Simon G. Potts 《Biological conservation》2011,(10):2424-2431

Arthropods that have a direct impact on crop production (i.e. pests, natural enemies and pollinators) can be influenced by both local farm management and the context within which the fields occur in the wider landscape. However, the contributions and spatial scales at which these drivers operate and interact are not fully understood, particularly in the developing world. The impact of both local management and landscape context on insect pollinators and natural enemy communities and on their capacity to deliver related ecosystem services to an economically important tropical crop, pigeonpea was investigated. The study was conducted in nine paired farms across a gradient of increasing distance to semi-native vegetation in Kibwezi, Kenya. Results show that proximity of fields to semi-native habitats negatively affected pollinator and chewing insect abundance. Within fields, pesticide use was a key negative predictor of pollinator, pest and foliar active predator abundance. On the contrary, fertilizer application significantly enhanced pollinator and both chewing and sucking insect pest abundance. At a 1 km spatial scale of fields, there were significant negative effects of the number of semi-native habitat patches within fields dominated by mass flowering pigeonpea on pollinators abundance. For service provision, a significant decline in fruit set when insects were excluded from flowers was recorded. This study reveals the interconnections of pollinators, predators and pests with pigeonpea crop. For sustainable yields and to conserve high densities of both pollinators and predators of pests within pigeonpea landscapes, it is crucial to target the adoption of less disruptive farm management practices such as reducing pesticide and fertilizer inputs.  相似文献   

乳糖在仔猪料中的最佳水平     

何忠武 《国外畜牧学(猪与禽)》2007,27(3):11-12

乳糖是一种缓泻药,也是提高仔猪早期生长性能的促进剂。因此,即使其成本能负担得起,也需要对刚断奶仔猪的饲喂量作详细的评估。  相似文献