Antcin B and its ester derivative from Antrodia camphorata induce apoptosis in hepatocellular carcinoma cells involves enhancing oxidative stress coincident with activation of intrinsic and extrinsic apoptotic pathway     

Hsieh YC  Rao YK  Whang-Peng J  Huang CY  Shyue SK  Hsu SL  Tzeng YM 《Journal of agricultural and food chemistry》2011,59(20):10943-10954

The triterpenoids methylantcinate B (MAB) and antcin B (AB), isolated from the medicinal mushroom Antrodia camphorata , have been identified as strong cytotoxic agents against various type of cancer cells; however, the mechanisms of MAB- and AB-induced cytotoxicity have not been adequately explored. This study investigated the roles of caspase cascades, reactive oxygen species (ROS), DNA damage, mitochondrial disruption, and Bax and Bcl-2 proteins in MAB- and AB-induced apoptosis of hepatocellular carcinoma (HCC) HepG2 cells. Here, we showed that MAB and AB induced apoptosis in HepG2 cells, as characterized by increased DNA fragmentation, cleavage of PARP, sub-G1 population, chromatin condensation, loss of mitochondrial membrane potential, and release of cytochrome c. Increasing the levels of caspase-2, -3, -8, and -9 activities was involved in MAB- and AB-induced apoptosis, and they could be attenuated by inhibitors of specific caspases, indicating that MAB and AB triggered the caspase-dependent apoptotic pathway. Additionally, the enhanced apoptotic effect correlates with high expression of Fas, Fas ligand, as well as Bax and decreased protein levels of Bcl-(XL) and Bcl-2, suggesting that both the extrinsic and intrinsic apoptosis pathways were involved in the apoptotic processes. Incubation of HepG2 cells with antioxidant enzymes superoxide dismutase and catalase and antioxidants N-acetylcysteine and ascorbic acid attenuated the ROS generation and apoptosis induced by MAB and AB, which indicate that ROS plays a pivotal role in cell death. NADPH oxidase activation was observed in MAB- and AB-stimulated HepG2 cells; however, inhibition of such activation by diphenylamine significantly blocked MAB- and AB-induced ROS production and increased cell viability. Taken together, our results provide the first evidence that triterpenoids MAB and AB induced a NADPH oxidase-provoked oxidative stress and extrinsic and intrinsic apoptosis as a critical mechanism of cause cell death in HCC cells.  相似文献   

Oocysts of Hepatozoon canis in Rhipicephalus (Boophilus) microplus collected from a naturally infected dog     

de Miranda RL  de Castro JR  Olegário MM  Beletti ME  Mundim AV  O'Dwyer LH  Eyal O  Talmi-Frank D  Cury MC  Baneth G 《Veterinary parasitology》2011,177(3-4):392-396

Canine hepatozoonosis is a tick-borne disease caused by protozoans of the genus Hepatozoon. Several tick species have been implicated as potential vectors. Therefore, extensive studies are needed to determine the 'natural' endemic cycle of this parasite. This paper presents the first report of the presence of Hepatozoon canis oocysts in Rhipicephalus (Boophilus) microplus collected from an infected dog.  相似文献   

Efficacy of dexamethasone,salbutamol, and reduced respirable particulate concentration on aerobic capacity in horses with smoke-induced mild asthma     

Stephanie L. Bond  Persephone Greco-Otto  Jacqueline MacLeod  Angelica Galezowski  Warwick Bayly  Renaud Léguillette 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(2):979-985

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Multicenter retrospective evaluation of ileocecocolic perforations associated with diagnostic lower gastrointestinal endoscopy in dogs and cats     

Vanessa L. Woolhead  Jacqueline C. Whittemore  Sarah A. Stewart 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(2):684-690

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Current progress in studying blackleg disease (Leptosphaeria maculans and L. biglobosa) of canola in Iran: Where do we stand now?     

Ali Zamanmirabadi  Roghayeh Hemmati  Aria Dolatabadian  Jacqueline Batley 《Plant pathology》2022,71(2):239-250

Blackleg, caused by a complex of Leptosphaeria species (L. maculans and L. biglobosa), is a fungal disease on Brassica species, especially important in canola (Brassica napus). Since the first report of L. biglobosa in Iran in 2007 and L. maculans in 2008, both species are now of major importance in Iran affecting 10 provinces and 30 regions, with a higher prevalence in the northern provinces of Mazandaran and Golestan. Despite the rapid progression of the disease and the emergence of new Leptosphaeria races in Iran, the research into this pathogen has not progressed at the same rate and is limited to phenotypic characterization studies, pathogenicity research, and to a lesser extent, disease management research. Given the rapid increase in canola cultivation in Iran and changes in the genetic diversity of the pathogen populations, it is likely that blackleg disease will increasingly become a severe threat to Iran’s canola production. Therefore, systematic and prospective studies, along with fundamental research on the pathogen's biology, epidemiology, and genetic diversity, would provide critical information for the development of disease management strategies. Here, we review the research that has been carried out to date on blackleg disease in Iran and describe the extent of progress towards disease control, especially in disease-prone regions.  相似文献   

Closing the compliance gap in marine protected areas with human behavioural sciences     

Brock J. Bergseth  Adrian Arias  Michele L. Barnes  Iain Caldwell  Amber Datta  Stefan Gelcich  Sam H. Ham  Jacqueline D. Lau  Cristina Ruano-Chamorro  Patrick Smallhorn-West  Damian Weekers  Jessica Zamborain-Mason  Joshua E. Cinner 《Fish and Fisheries》2023,24(4):695-704

Advocates, practitioners and policy-makers continue to use and advocate for marine protected areas (MPAs) to meet global ocean protection targets. Yet many of the worlds MPAs, and especially no-take MPAs, are plagued by poaching and ineffective governance. Using a global dataset on coral reefs as an example, we quantify the potential ecological gains of governing MPAs to increase compliance, which we call the ‘compliance gap’. Using ecological simulations based on model posteriors of joint Bayesian hierarchical models, we demonstrate how increased compliance in no-take MPAs could nearly double target fish biomass (91% increases in median fish biomass), and result in a 292% higher likelihood of encountering top predators. Achieving these gains and closing the compliance gap necessitates a substantial shift in approach and practice to go beyond optimizing enforcement, and towards governing for compliance. This will require engaging and integrating a broad suite of actors, principles, and practices across three key domains: (i)) harnessing social influence, (ii) integrating equity principles, and (iii) aligning incentives through market-based instruments. Empowering and shaping communication between actor groups (e.g., between fishers, practitioners, and policy-makers) using theoretically underpinned approaches from the behavioural sciences is one of the most essential, but often underserved aspects of governing MPAs. We therefore close by highlighting how this cross-cutting tool could be further integrated in governance to bolster high levels of compliance in MPAs.  相似文献   

Diagnostic and public health investigation of Mycobacterium tuberculosis infection in a dog in Ontario,Canada     

Luke A. J. Haydock  Anthony C. G. Abrams-Ogg  J. Scott Weese  Michael R. Goldstein  Amy B. Clifford  Adrian Sebastian  Elizabeth H. Rea  Frances B. Jamieson  Carla Duncan  Olga Andrievskaia  Mirjana Savic  Durda Slavic  Robert A. Foster  Christopher J. Greenwood  Tamara L. MacDonald  Jacqueline E. Scott  Andrea Sanchez 《Journal of veterinary diagnostic investigation》2022,34(2):292

A 4-y-old, female mixed-breed dog was presented to the Ontario Veterinary College for further evaluation of multiple pulmonary and hepatic masses, intrathoracic lymphadenitis, and recent development of a pyogranulomatous pleural effusion. Along with other comprehensive tests, a thoracic lymph node biopsy was performed, and Mycobacterium tuberculosis complex infection was confirmed by real-time PCR. The dog’s condition declined post-operatively, and euthanasia was elected. Postmortem examination confirmed severe granulomatous pneumonia, hepatitis, intrathoracic and intraabdominal lymphadenitis, omentitis, and nephritis. Line-probe assays performed on samples collected postmortem confirmed the species as M. tuberculosis. 24-loci MIRU-VNTR genotyping, spoligotyping, and whole-genome sequencing revealed relations to known human isolates, but no epidemiologic link to these cases was investigated. Given the concern for potential human exposure during this animal’s disease course, a public health investigation was initiated; 45 individuals were tested for M. tuberculosis exposure, and no subsequent human infections related to this animal were identified. Our case highlights the need for more readily available, minimally invasive testing for the diagnosis of canine mycobacteriosis, and highlights the ability of canid species to act as potential contributors to the epidemiology of M. tuberculosis infections.  相似文献   

Feline house soiling: elimination and marking behaviors.     

Jacqueline C Neilson 《Veterinary Clinics of North America: Small Animal Practice》2003,33(2):287-301

Although challenging at times, managing cats with elimination issues can be rewarding. The frustration in management of these cases is often a result of the lack of a proper diagnosis and random treatment application. A systematic approach to these cases should help to achieve treatment success. Also, new information should lead to further advancements in treatment.  相似文献   

Surgical treatment of chronic penile prolapse in Vietnamese pot‐bellied pigs: 5 cases (2016–2017)     

Laia Reig  Jacqueline A. Hill  Anna Mitchell  Galina Hayes  Ariel N. Schlag  Susan L. Fubini 《Veterinary surgery : VS》2019,48(5):890-896

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Persistent haematuria and proteinuria due to glomerular disease in related Abyssinian cats     

White JD  Norris JM  Bosward KL  Fleay R  Lauer C  Malik R 《Journal of Feline Medicine and Surgery》2008,10(3):219-229

Eight cases of glomerular disease in young, related Abyssinian cats are described. Haematuria was the most consistent feature. Six cats developed the nephrotic syndrome. The short-term prognosis was good for cats with haematuria and fair for cats with the nephrotic syndrome as oedema resolved in three of the six cats. Light microscopic examination of renal biopsies from three cats was considered normal or revealed only mild abnormalities. In the three cases subjected to necropsy, histological abnormalities included mild mesangial hypercellularity and adhesions between the glomerular tuft and Bowman's capsule consistent with a focal proliferative glomerulopathy. Further investigation into this glomerulopathy will require ultrastructural and immunohistochemical studies to characterise the glomerular abnormality and genetic analyses to investigate its potential to be an inherited disease. Glomerular disease, potentially a familial one, should be considered in the investigation of persistent haematuria or proteinuria in Abyssinian and related cats.  相似文献