Pancytopenia in a cat with visceral leishmaniasis     

Ricardo Marcos  Marta Santos  Fernanda Malhão  Rui Pereira  Ana Cristina Fernandes  Luís Montenegro  Paola Roccabianca 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(2):201-205

Abstract: A 4‐year‐old, domestic shorthair, female spayed cat was presented for decreased appetite and depression. Severe pancytopenia with erythrocyte autoagglutination was found. The cat was seronegative for feline immunodeficiency and leukemia viruses. Immune‐mediated hemolytic anemia was suspected but no response to treatment with a blood transfusion, enrofloxacin, and prednisone was observed. Blood and bone marrow smears obtained 11 days later contained Leishmania amastigotes in the cytoplasm of neutrophils and macrophages, respectively. Serologic and PCR testing of peripheral blood confirmed infection with Leishmania infantum. Despite treatment, the cat worsened clinically and was euthanized. At necropsy, visceral dissemination of the parasite was confirmed. The findings in this case indicate that visceral leishmaniasis should be considered as a differential diagnoses in cats with pancytopenia in areas endemic for Leishmania. In addition, amastigotes may be observed in peripheral blood neutrophils.  相似文献   

Effect of floor cooling and dietary amino acids content on performance and behaviour of lactating primiparous sows during summer     

B.A.N. Silva  R.F.M. Oliveira  J.L. Donzele  H.C. Fernandes  A.L. Lima  D. Renaudeau  J. Noblet 《Livestock Science》2009,120(1-2):25-34

Fifty nine primiparous sows PIC Camborough 23 were distributed in a completely randomized 2 × 2 (with and without floor cooling × two dietary treatments) factorial design with 16 sows/treatment, each sow being considered as an experimental unit. Four replicates of sixteen sows each were used during the trial with the objective of evaluating the effects of floor cooling and the use of dietary amino acid contents on their performance and behaviour during summer. The sows were distributed among the treatments according to body weight and backfat thickness after farrowing. The sows were maintained in the experiment until weaning at 21 days of lactation. The two experimental diets supplied the same levels of crude protein (22%), metabolizable energy (ME; 14.65 MJ/kg) and levels of essential digestible AA relative to digestive lysine and differed according to the digestible lysine to ME ratio (0.75 vs. 0.82 g/MJ of ME). The temperature of the water circulating in the cooled floor was maintained at about 17 °C. Based on the average minimum and maximum temperatures (21.5 and 29.5 °C) obtained during the experimental trial, it can be assumed that the sows were exposed to periods of heat stress. The replicate and the interaction between replicate and treatment effects on all the measurements were not significant. Similarly, no effect of diet or interaction between diet and floor cooling system was found for all criteria measured. An effect (P < 0.05) of floor cooling on average daily feed intake was observed and floor cooling sows showed a higher average (P < 0.05) digestible lysine (61.5 vs. 51.8 g/d) and ME (78.2 vs. 65.9 MJ/d) intakes. The sows submitted to floor cooling showed, consistently, higher absolute values for average weight (+ 8.5 kg) and backfat (+ 0.75 mm) at weaning, compared with the control sows. The sows submitted to the cooled floor showed a shorter (P < 0.01) weaning-to-oestrus interval. The piglet and litter's daily weight gain (DWG), average weight at weaning (AWW) and total weight gain during lactation (TWG) were higher (P < 0.01) for the floor cooling sows. The floor cooling sows showed a higher (P < 0.01) daily milk production. The respiratory rate and rectal temperature values were lower (P < 0.01) for the floor cooling sows. There were differences (P < 0.01) on the cutaneous temperatures measured on the different parts of the sow's body, with the animals submitted to the cooled floor having lower values. The sows submitted to floor cooling spent less (P < 0.01) time in lateral recumbency inactive, more time nursing (P < 0.05) and more time feeding (P < 0.01) compared with control sows. The floor cooling under the sows increased daily feed intake and lysine intake, leading to a lower body weight loss, a lower weaning-to-oestrus interval and also improved nursing behaviour of the sows, leading to a higher milk production and, consequently, higher weight gains of piglets and litter during the lactation period.  相似文献   

Rearing temperature affects Senegalese sole (Solea senegalensis) larvae protein metabolic capacity     

Catarina Campos  M. Filipa Castanheira  Sofia Engrola  Luísa M. P. Valente  Jorge M. O. Fernandes  Luís E. C. Conceição 《Fish physiology and biochemistry》2013,39(6):1485-1496

The present work examined the short- and long-term effects of three rearing temperatures on protein metabolism and growth trajectories of Senegalese sole larvae using ¹⁴C-labelled Artemia protein as feed. A first feeding trial was performed on larvae reared at 15, 18 and 21 °C (at 26, 17 and 14 days post-hatching (dph), respectively) and a second trial conducted on post-larvae after transfer to the same rearing temperature (~20 °C) (49, 35 and 27 dph, in larvae initially reared at 15, 18 and 21 °C, respectively). Temperature greatly influenced larvae relative growth rate (RGR) and survival, since growth at 15 °C was severely depressed. Protein digestibility and retention was highest at 18 °C during the first trial (85.35 ± 1.16 and 86.34 ± 2.33 %, respectively). However, during the second trial, post-larvae from 15 °C had the highest feed intake and protein digestibility (3.58 ± 1.54 and 75.50 ± 1.35 %, respectively), although retention was similar between treatments. Furthermore, after transfer to 20 °C larvae from 15 °C experienced compensatory growth, which was observed until 121 dph, and confirmed by RGR values, which were significantly higher at 15 ºC than at 21 ºC or 18 ºC. Results from the present study show that Solea senegalensis larval development, survival and protein digestion and retention are highly affected by thermal history.  相似文献   

AHI POKE (RAW TUNA SALAD) CONSUMPTION AND CONSUMER CHARACTERISTICS IN HAWAII     

Pedro M. Fernandes da Costa  Minling Pan 《Aquaculture Economics & Management (Blackwell Science)》2013,17(4):302-315

Ahi poke (raw tuna salad) has a significant role in Hawaiian culture and economy. A consumer survey in Hawaii was used to examine consumers’ purchasing intentions for ahi poke. A censored regression analysis was conducted to analyze these purchases and connect with various consumer characteristics. Results show that many consumers eat ahi poke frequently and different consumer profiles will lead to large differences in the quantity consumed. Information obtained in this study may help producers and retailers to better target their marketing strategies and increase sales.  相似文献   

Release of doxycycline through cellulose acetate symmetric and asymmetric membranes produced from recycled agroindustrial residue: Sugarcane bagasse     

Guimes Rodrigues Filho  Sabrina Dias RibeiroCarla da Silva Meireles  Leandro Gustavo da SilvaReinaldo Ruggiero  Moacir Fernandes Ferreira JuniorDaniel Alves Cerqueira  Rosana Maria Nascimento de AssunçãoMara Zeni  Patricia Polleto 《Industrial Crops and Products》2011,33(3):566-571

Cellulose acetate is one of the components employed in drug controlled-release systems in the form of membranes. The aim of this study was to examine the controlled-release of doxycycline employing cellulose acetate symmetric and asymmetric membranes as matrices. The cellulose triacetate was produced from sugarcane bagasse through a homogeneous acetylation reaction, using acetic acid as the solvent, acetic anhydride as the acetylating agent and sulfuric acid as the catalyst. The viscosity average molecular weight of the cellulose acetate produced was 39,000 g mol⁻¹. The symmetric membranes were produced using a system solvent of dichloromethane/ethanol (9:1, v/v) and the asymmetric membranes were produced from the same solvent system and 10% of water. For the formulation of both, 5% of doxycycline was used. The membranes were characterized by thermal analysis (DSC and TGA) and scanning electron microscopy SEM. The release of doxycycline through cellulose triacetate matrices was examined using spectrophotometric analysis in the ultraviolet-visible region, at 275 nm. The results revealed that asymmetric membranes release 80% of the drug in 100 min, while symmetric membranes release 14% of the drug during the same time interval.  相似文献   

Biosorption of macronutrients by Brazilian tropical peats     

Camila Almeida Melo  Lílian Karla de Oliveira  Leonardo Fernandes Fraceto  André Henrique Rosa 《Communications in Soil Science and Plant Analysis》2018,49(12):1530-1539

The objective of this work was to evaluate the adsorption of macronutrients calcium, potassium, magnesium, nitrogen, and phosphorus in two Brazilian tropical peat samples, investigating the effect of pH and determining the kinetics of the adsorption process. Two different Brazilian tropical peat samples were characterized using FTIR, TG, and SEM techniques. Different pH conditions were tested, as well as different mass concentrations of the peats. Differences in the chemical structures of the peat samples directly influenced the adsorptive capacities for the macronutrients. The adsorptive capacity for nitrogen was highest at pH 3, while the best adsorption of calcium and potassium was obtained at pH 6. The best fit to the data was provided by the pseudo-second-order model, which confirmed the rapid adsorption of calcium by both peats.  相似文献   

Evaluation of Magnetic Coagulant (α-Fe<Subscript>2</Subscript>O<Subscript>3</Subscript>-MO) and its Reuse in Textile Wastewater Treatment     

Tássia Rhuna Tonial dos Santos  Gustavo Affonso Pisano Mateus  Marcela Fernandes Silva  Carolina Sayury Miyashiro  Leticia Nishi  Murilo Barbosa de Andrade  Márcia Regina Fagundes-Klen  Raquel Guttieres Gomes  Rosângela Bergamasco 《Water, air, and soil pollution》2018,229(3):92

The textile industries are characterized as one of the biggest consumers of potable water and chemical products throughout its process, being responsible for the elevated wastewater generation with intense coloration and wide polluting potential. In this context, the present study proposes the development and application of a new coagulant material for textile wastewater treatment. The proposed coagulant (α-Fe₂O₃-MO) was composed by hematite nanoparticles (α-Fe₂O₃) obtained by a simple non-pollutant methodology, associated with Moringa oleifera (MO) seeds saline extract compounds. Coagulation/flocculation (CF) efficiency was evaluated by removal of physicochemical parameters such as apparent color, turbidity, and compounds with absorption at UV_254nm (UV_254nm) through CF tests carried out on Jar test equipment and sedimentation carried out in the presence and absence of external magnetic field (600 k Am^?1). Kinetics sedimentation was from 0 to 90 min. The use of this new coagulant allowed the removal of 92.37% for apparent color, 91.43% for turbidity, and 46.09% for UV_254nm, indicating that the proposed coagulant association was efficient in the treatment of this type of wastewater under external magnetic field with only 10 min of sedimentation. In addition, the resulting sludge from CF process was tested as base material for a new coagulant synthesis, demonstrating great reuse potential. Therefore, the new proposed coagulant, composed of α-Fe₂O₃ and the compounds present in the seed extract of MO, has applicability for textile wastewater treatment demonstrating high removal rate for all evaluated parameters with cost reduction in the proposed treatment for this wastewater.  相似文献   

Biofilms of Candida spp. from the ocular conjunctiva of horses with reduced azole susceptibility: a complicating factor for the treatment of keratomycosis?          下载免费PDF全文

Raimunda Sâmia Nogueira Brilhante  Paula Vago Bittencourt  Débora de Souza Collares Castelo‐Branco  Glaucia Morgana de Melo Guedes  Jonathas Sales de Oliveira  Lucas Pereira Alencar  Rossana de Aguiar Cordeiro  Mariana Pinheiro  Evilázio Fernandes Nogueira‐Filho  Waldemiro de Aquino Pereira‐Neto  José Júlio Costa Sidrim  Marcos Fábio Gadelha Rocha 《Veterinary ophthalmology》2017,20(6):539-546

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Immortalization and characterization of a new canine mammary tumour cell line FR37‐CMT          下载免费PDF全文

L. R. Raposo  C. Roma‐Rodrigues  P. Faísca  M. Alves  J. Henriques  M. C. Carvalheiro  M. L. Corvo  P. V. Baptista  A. J. Pombeiro  A. R. Fernandes 《Veterinary and comparative oncology》2017,15(3):952-967

Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37‐CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD‐SCID mice. FR37‐CMT cell line has a stellate or fusiform shape, displays the ability to reorganize the collagen matrix, expresses vimentin, CD44 and shows the loss of E‐cadherin which is considered a fundamental event in epithelial to mesenchymal transition (EMT). The up‐regulation of ZEB1, the detection of phosphorylated ERK1/2 and the downregulation of DICER1 and miR‐200c are also in accordance with the mesenchymal characteristics of FR37‐CMT cell line. FR37‐CMT shows a higher resistance to cisplatin (IC₅₀>50 µM) and to doxorubicin (IC₅₀>5.3 µM) compared with other CMT cell lines. These results support the use of FR37‐CMT as a new CMT model that may assist the understanding of the molecular mechanisms underlying EMT, CMT drug resistance, fostering the development of novel therapies targeting CMT.  相似文献   

Yield and nutritional requirements of cassava in response to potassium fertilizer in the second cycle     

Adalton Mazetti Fernandes  Bruno Gazola  Jesion Geibel da Silva Nunes  Emerson Loli Garcia  Magali Leonel 《Journal of plant nutrition》2017,40(20):2785-2796

Potassium (K) is one of the most absorbed nutrients by cassava because it acts on the synthesis and starch accumulation in the storage roots. Here, we show that K application at the beginning of the second vegetative cycle of cassava submitted to shoot pruning increased the yield of roots and starch, and the nutrient demand of plants. Application of 45–89 kg ha^?1 dipotassium oxide (K₂O) in the second cycle increased the yield of storage roots and starch from 36–49% and K applied at this time had a greater effect on the synthesis and allocation of starch in the storage roots. K supply increased the accumulation of N and S by 2.0- to 3.0-fold and the accumulation of other nutrients by 1.4- to 1.7-fold. The removal of phosphorus (P), manganese (Mn), and zinc (Zn) by storage roots was not affected by K application, whereas the removal of other nutrients increased by 1.3- to 4.3-fold.  相似文献