Genetic relationships among resynthesized,semi-resynthesized and natural <Emphasis Type="Italic">Brassica napus</Emphasis> L. genotypes     

Katarzyna Sosnowska  Teresa Cegielska-Taras  Alina Liersch  Wojciech M. Karłowski  Jan Bocianowski  Laurencja Szała  Katarzyna Mikołajczyk  Wiesława Popławska 《Euphytica》2017,213(9):212

The allopolyploidization event that created cultivated oilseed rape Brassica napus L, followed by intense breeding, reduced its genetic diversity. Resynthesized (RS) B. napus L. obtained by interspecific hybridization between genotypes of B. rapa L. and B. oleracea L. can be a valuable source for broadening genetic diversity in cultivated oilseed rape. In this study, we determined the extent of DNA polymorphism among natural accessions of oilseed rape, resynthesized B. napus, their parental species and double-low quality semi-RS lines carrying the Rfo gene. Using 10 selected primer combinations, 522 polymorphic AFLP markers were scored in the complete set of 100 Brassica sp. To detect relationships between these genotypes, a cluster analysis was performed using the Jaccard’s distance. Resynthesized allopolyploids clustered directly between their diploid parents. Cultivated accessions of oilseed rape created a compact group away from resynthesized allopolyploids as well as semi-RS lines. The natural oilseed rape group, which consists of 49 cultivars and breeding lines of oilseed rape, is characterized by lower genetic diversity than the group of 33 accessions of resynthesized oilseed rape, and the analysis showed that the double-low quality semi-RS lines represent a specific genetic variation of B. napus. The de novo resynthesized B. napus lines and the semi-RS lines of double-low quality generated from them, provide a significant opportunity for enrichment the gene pool of oilseed rape.  相似文献   

Identification of S-haplotypes of European cultivars of sour cherry     

Anna Lisek  Danuta Kucharska  Agnieszka Głowacka  Elżbieta Rozpara 《The Journal of Horticultural Science and Biotechnology》2017,92(5):484-492

The sour cherry is known to exhibit the phenomenon of gametophytic self-incompatibility which prevents self-fertilization. In sour cherry, besides self-incompatible cultivars, there often occur self-compatible cultivars. This is due to the occurrence of natural mutations of the S-RNase or SFB genes and, consequently, loss of functionality of S-alleles. Here we present the results of the identification of S-haplotypes of 21 cultivars of sour cherry from various regions of Europe. The analyses were performed using methods based on the amplification of intron I and intron II of the S-RNase gene and fragments specific to the individual alleles of the S-RNase or SFB genes. The tested cultivars were found to contain 15 S-haplotypes: S₁, S_1?, S₄, S₆, S_6m, S_6m2, S₉, S₁₂, S₁₃, S_13?, S₂₆, S₃₅, S_36a, S_36b, and S_36b2. The most frequently occurring S-haplotypes were S_13? (61.9%), S_36a (57.1%), and S₂₆ (47.6%). On the basis of the results, 17 of the 21 cultivars were deduced to be self-compatible. The results will be of use in the production of sour cherry fruit by facilitating the selection of suitable pollinating cultivars. The results are also expected to be useful in the breeding of new cultivars of sour cherry when selecting genotypes for crosses.  相似文献   

Historical changes in land use influence current habitat preferences of large herbivores     

Perzanowski  Kajetan  Januszczak  Maciej  Łopucki  Rafał 《Landscape Ecology》2019,34(10):2251-2259

Landscape Ecology - Predicting habitat use patterns is a key issue in the management of large herbivore populations. Particularly, indicators providing a model of the spatial distribution of a...  相似文献   

Synthesis and phytotoxicity of new ionic liquids incorporating chiral cations and/or chiral anions     

Bałczewski P  Bachowska B  Białas T  Biczak R  Wieczorek WM  Balińska A 《Journal of agricultural and food chemistry》2007,55(5):1881-1892

The aim of this work was to synthesize chiral ionic liquids as chiral solvents for organic synthesis and to evaluate the phyto(eco)toxicity of the new products and starting N-alkylimidazoles and their potential environmental influence on soil and plants. Chiral ionic liquids containing anions such as Cl-, Br-, TsO-, PF6(-), NO3(-), CF3SO3(-), and (+)- and (-)-C6H5CH(OH)C(O)O- were synthesized using (-)-(1R)-6,6-dimethylbicyclo[3.1.1]hept-2-ene-2-ethyl [(-)-(1R)-nopyl] halides (X = Cl, Br) and tosylate in 62-100% yields. The chloride 7 and the nitrate 13 ionic liquids possessed a toxicity dependent on the applied concentration. The lowest concentration causing a distinct reduction in plant germination/growth was 100 mg/kg. Spring barley better tolerated the ionic liquids (200 mg/kg) than common radish (100 mg/kg). The nitrate liquid did not exhibit an inhibiting effect on the germination ability of seeds. The starting N-methylimidazole used in lower concentrations (1 and 10 mg/kg of soil dry weight) was not phytotoxic, in contrast to higher doses (>1000 mg/kg).  相似文献   

Temperature-dependent resistance to potato virus M in potato (Solanum tuberosum)     

Beata Tatarowska  Jarosław Plich  Dorota Milczarek  Bogdan Flis 《Plant pathology》2020,69(8):1445-1452

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Effect of dietary <Emphasis Type="SmallCaps">l</Emphasis>-tryptophan on cannibalism,survival and growth in pikeperch <Emphasis Type="Italic">Sander lucioperca</Emphasis> (L.) post-larvae     

Jarosław Król  Zdzisław Zakęś 《Aquaculture International》2016,24(2):441-451

The effect of supplemented commercial diets with crystalline l-tryptophan (TRP—5, 10 and 20 g TRP kg^?1) on cannibalism, survival and growth parameters of pikeperch post-larvae (Sander lucioperca) was evaluated. Fifteen-day-old pikeperch larvae (mean weight—6.8 mg) were reared during the next 28 days (20.5 °C, 16L:8D) in glass aquaria in a recirculating aquaculture system. The enzyme-linked immunosorbent assay showed that TRP-supplemented diets were effective in increasing the levels of serotonin (5-HT) in the body tissue of pikeperch. TRP supplementation resulted in a slight decrease in both types of cannibalism, although the reduction in cannibalism impact did not amount to more than a few percent. TRP treatment had no significant influence on the final survival of pikeperch post-larvae (ranged from 20.1 ± 12.4 to 29.0 ± 12.9 %). However, contrary to the earlier studies conducted on other fish species, no significant difference in the growth rates and feeding behavior of pikeperch between TRP-fed and control group were found. The final body weight and growth rate ranged from 0.211 ± 0.014 to 0.243 ± 0.016 and from 12.19 ± 0.38 to 12.76 ± 0.35 % day^?1, respectively. To our knowledge, this is the first study reporting the effects of TRP supplementation on the cannibalism–survival–growth relations in fish in the post-larval stage.  相似文献   

The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch,Sander lucioperca (L.)          下载免费PDF全文

Daniel Żarski  Sławomir Krejszeff  Dariusz Kucharczyk  Katarzyna Palińska‐Żarska  Katarzyna Targońska  Krzysztof Kupren  Pascal Fontaine  Patrick Kestemont 《Aquaculture Research》2015,46(2):324-334

The aim of this article was to analyse the process of pikeperch, Sander lucioperca, egg swelling and to apply tannic acid to eliminate egg stickiness at different moments of the swelling process on artificially obtained eggs. The first experiment involved observation of egg swelling process and the second determined the effect of temperature (12, 14 and 16°C) on the egg swelling rate. The third experiment involved elimination of egg stickiness in a tannin solution (0.75 g L^?1) where eggs were submerged in a solution for 0.5, 1, 2 and 5 min – 5, 10, 15, 20, 25 and 30 min following gamete activation. The results indicate that the pikeperch egg swelling process lasts 30 min. It was found that the temperature did not affect the process duration. The results of the third experiment showed that the effectiveness of tannic acid application in egg stickiness elimination increases with time. The best result was obtained in groups of eggs submerged for 1 and 2 min (86.5% and 80.5% of larvae were obtained respectively) 30 min following the gamete activation. The results presented in this study for the first time indicate the possibility of highly effective procedure of egg stickiness elimination with tannic acid in pikeperch aquaculture.  相似文献   

Sediment load variability in response to climate and land use changes in a Carpathian catchment (Raba River,Poland)     

Szalińska  Ewa  Orlińska-Woźniak  Paulina  Wilk  Paweł 《Journal of Soils and Sediments》2020,20(6):2641-2652

Journal of Soils and Sediments - This study analyzes the variability of sediment loads under variant climate change and land use scenarios in a Carpathian catchment with a dam reservoir. The areas...  相似文献   

Effect of Addition of Different Acetic Acid Concentrations on the Quality of Marinated Herring     

Mariusz Szymczak  Edward Kołakowski  Katarzyna Felisiak 《Journal Of Aquatic Food Product Technology》2013,22(6):566-581

This study investigated the effect of acetic acid concentrations from 3 to 8% on the physicochemical and sensory quality of marinated Atlantic and Baltic herring from fresh and frozen-thawed material. The increased concentration of acetic acid was found to exert a strong impact on decreased sensory scores, weight yield, and pH value as well as on an increased brightness parameter of meat. In turn, moisture, protein hydrolysis products (PHB), total volatile bases (TVB) content, and hardness parameter were affected by the higher addition of acid to a lesser extent. Enhanced protein hydrolysis was observed only at acetic acid concentration of 5% or 5–8%, whereas there was a decrease in the content of TVB only at an acid concentration of up to 4–5%. The marinated frozen-thawed fillets, compared to the fresh ones, required a greater addition of acid in the brine, by 1.5%, to reach a similar pH value. The freezing of fish contributed also to a decrease of PHB and free hydroxyproline, along with an increased concentration of acetic acid. The marinades from frozen-thawed herring contained more TVB, had worse color, and hardness parameters, as well as less yield.  相似文献   

Influence of zearalenone on reproductive system cell proliferation in gilts   总被引：1，自引：0，他引：1  

Obremski K  Gajecki M  Zwierzchowski W  Zielonka L  Otrocka-Domagała I  Rotkiewicz T  Mikołajczyk A  Gajecka M  Polak M 《Polish journal of veterinary sciences》2003,6(4):239-245

Zearalenone (ZEA) is a macrocyclic lactone, estrogenic, diet-depending and fusaric micotoxin, which is produced on many kinds of cereals and feeds in the favourable conditions of humidity and temperature. The structure of ZEA is similar to the structure of estrogens and it enables binding to the estrogenic receptors. The stimulation of protein synthesis in the cells of the reproductive system, which causes intensification of cell proliferation, is one of the effects of ZEA actions. Oedema and vulva reddening are the clinical, external signs of ZEA intoxication in pigs. The aim of this study was to designate the degree of reproductive cell proliferation after low doses of ZEA were applied per os in sexually immature gilts with simultaneous monitoring of zearalenone and alpha-zearalenol levels in peripheral blood. The following were observed in the gilts examined fluctuations of zearalenone and alpha-zearalenol levels in blood, which were connected with entero-hepatic circulation and also numerous histopathological changes in ovarian follicle structure. These changes were present in the reproductive system of sexually immature gilts with a big contribution of PCNA-positive cells. The studies show that zearalenone application in sexually immature gilts caused ovarian follicle atresia and apoptoso-like changes in granule cells. Intensified cell proliferation, which was expressed with the growth of PCNA index, was observed in uterus and oviduct.  相似文献