全文获取类型
收费全文 | 17084篇 |
免费 | 47篇 |
专业分类
林业 | 3688篇 |
农学 | 1320篇 |
基础科学 | 141篇 |
2902篇 | |
综合类 | 737篇 |
农作物 | 2127篇 |
水产渔业 | 1910篇 |
畜牧兽医 | 1285篇 |
园艺 | 1123篇 |
植物保护 | 1898篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 13篇 |
2021年 | 35篇 |
2020年 | 24篇 |
2019年 | 36篇 |
2018年 | 2761篇 |
2017年 | 2718篇 |
2016年 | 1205篇 |
2015年 | 84篇 |
2014年 | 61篇 |
2013年 | 53篇 |
2012年 | 855篇 |
2011年 | 2195篇 |
2010年 | 2130篇 |
2009年 | 1286篇 |
2008年 | 1357篇 |
2007年 | 1617篇 |
2006年 | 66篇 |
2005年 | 127篇 |
2004年 | 128篇 |
2003年 | 173篇 |
2002年 | 85篇 |
2001年 | 7篇 |
2000年 | 44篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 12篇 |
1992年 | 8篇 |
1990年 | 2篇 |
1989年 | 5篇 |
1988年 | 11篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1977年 | 5篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 4篇 |
1967年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Yongju Zhao Jiahua Zhang Hong Wei Xinming Sun Biao Mu Mingju Yu Lingbing Wang 《Tropical animal health and production》2010,42(6):1257-1262
Two experiments were undertaken to select the efficient method applied for goat estrous synchronization. In experiment 1,
a total of 120 does (Capra hircus) were divided into five groups with a randomized block design, and the does of treatment 1 were synchronized by intravaginal
sponges impregnated with 30 mg Levonorgestrel inserted for 10 days. Does of treatments 2, 3, and 4 were treated with further
injection of 25 IU follicle-stimulating hormone (FSH), 0.05 mg prostaglandin F2 alpha (PGF2α), and 25 IU FSH + 0.05 mg PGF2α at sponge withdrawal, respectively. The does in the control group (n = 40) without estrous synchronization treatment and natural estrous does were observed. In experiment 2, a total of 140 does
in five goat farms in breeding and non-breeding seasons were treated with the selective efficient procedure. The results presented
that all the employed treatments were capable of inducing and synchronizing estrous goats. According to estrous response and
economy, the use of intravaginal sponges impregnated with 30 mg Levonorgestrel and 0.05 mg PGF2α (treatment 3) is the first choice for estrous synchronization, and 95.0% of synchronized does demonstrated estrus, which
was significantly higher than that of treatment 1 (P < 0.05) and control group (P < 0.01). The percentages of ovulating of treatments 3 and 4 were the same (95.0%), which were significantly higher than that
of treatment 1 (P < 0.01). The ovulation rates among different groups were not significant (P > 0.05). When the selective procedure was applied to five goat farms, 85.7% (120/140) of does demonstrated estrus, and the
kidding percentage, litter size, and prolificacy rate were 53.6%, 0.95, and 177%, respectively. 相似文献
82.
George Rigos Alexandros Samartzis Morgane Henry Eleni Fountoulaki Efthimia Cotou John Sweetman Simon Davies Ioannis Nengas 《Aquaculture International》2010,18(6):1093-1104
The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead
sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet).
Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final
weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells,
haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst
activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue
distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200
ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly
affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory
burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300
ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication
that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point
of view. 相似文献
83.
84.
In order to identify the factors providing the triazolopyrimidines with a wide range of selectivity amongst agriculturally important plant species, studies were made on the uptake and metabolism of a representative compound, N-(2,6-dichlorophenyl)-5,7-dimethyl-1,2,4-triazolo[1,5-a]pyrimidine- 2-sulfonamide. This compound is much more phytotoxic to dicots than to monocots. Experiments showed that variations in leaf morphologies and geometries relative to spray applications resulted in different herbicide dosages applied to plant species used in selectivity evaluations. However, this parameter did not have a statistically significant correlation to herbicide selectivity measurements. Characterization of the metabolic pathways showed that the major herbicide metabolites involved methyl hydroxylation or hydroxylation of the aniline ring followed by glucose conjugation. Since these metabolites were not exclusive to either monocots or dicots, herbicide selectivity does not appear dependent on the pathway of metabolism. Studies on the kinetics of metabolism suggest instead that metabolic rate was important. Monocots metabolized the herbicide rapidly and generally were tolerant while the opposite was true of dicots. 相似文献
85.
Cecilia Mónica Galosi Viviana Cid de la Paz Leandro Carlos Fernández Javier Pablo Martinez María Isabel Craig María Barrandeguy María Elisa Etcheverrrigaray 《Journal of veterinary diagnostic investigation》2005,17(5):500-502
This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion. 相似文献
86.
Pablo Gómez-Ochoa Francisco Javier Miana-Mena María Jesús Mu?oz Encarna Cativiela Francisco Gómez 《Canadian journal of veterinary research》2005,69(4):309-312
The purpose of this study was to isolate and cultivate a subpopulation of pluripotent stem cells (PSCs) from the peripheral blood of rabbits, which are frequently used in veterinary research as an animal model. Pluripotent stem cells, as described in human beings, are fibroblast-like cells that exhibit a CD34 marker, specific from other hematopoietic stem cells. Commonly used human commercial media has been researched for culturing rabbit PSCs. These findings allow us to contemplate the direct application of this simple and standardized methodology in several areas of study, such as of the pharmacological effect of many drugs on hematopoietic cells, veterinary practice, and even the study of new strategies in cellular therapy for some human diseases. 相似文献
87.
Marianne J. Huisman Erik Jongedijk Dinie Posthumus-Lutke Willink Frank Van Der Wilk Ben J. C. Cornelissen 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):29-36
To engineer resistance against potato virus X (PVX), the viral coat protein (CP) gene has been introduced into two potato cultivars. Stable expression of the gene in transgenic clones throughout the growing season has been obtained and resulted in considerably increased virus resistance. With varying frequencies depending on the original cultivar used, true-to-type PVX resistant transgenic clones have been obtained. Since deviant light sprout characteristics were invariably associated with aberrations in plant phenotype, they can be used in procedures to early screen for deviations. Furthermore, it has been possible to unequivocally discriminate between the original untransformed and independent transgenic cultivars. Although no relation has been found between the presence, if any, of the CP of potato virus Y (PVY) or potato leafroll virus (PLRV) in CP gene transgenic potato, appreciable levels of resistance to these viruses has been obtained. This suggests that the mechanism by which a viral CP gene in the potato genome evokes resistance, differs amongst various viruses. 相似文献
88.
O. M. van Andel 《European journal of plant pathology / European Foundation for Plant Pathology》1968,74(1):113-120
Experiments on the effect of various compounds with plant growth regulating activity led to the hypothesis that conditions inhibitory to indoleacetic acid (IAA) action or leading to a decrease in the IAA level in cucumber seedlings would be unfavourable for the development of cucumber scab, caused byCladosporium cucumerinum. Susceptibility decreased as the result of treatment with growth retardants, which cause an increase in IAA-oxidase activity in the plants, whereas application of indoleacetic acid or compounds expected to decrease the rate of oxidative breakdown of IAA in the seedlings resulted in an increase of susceptibility. Growing the plants under various periods of illumination also influenced both the susceptibility of the plants and the IAA-oxidase activity in the hypocotyl tissue.To obtain further information about the relation between the increase in resistance and the increase in the rate of IAA oxidation, the effect of one compound, viz. L-threo-β-phenylserine (abbr. phenylserine) was studied in more detail. The rate of oxidative breakdown of IAA was increased in extracts of hypocotyls and cotyledons of plants treated with phenylserine as compared with extracts of tissue of untreated plants. Extracts of phenylserine-treated plants were found to contain a higher cofactor and/or a lower inhibitor content than those of control plants. The substances involved were not identified, but this result may indicate a shift in the concentrations of various phenols in the tissue.The question must remain open whether a shift in the concentration of phenols some-how effected by phenylserine treatment determines both degree of susceptibility and rate of IAA oxidation independently, or whether the rate of IAA breakdown and the resulting change in IAA content in the plants is directly related to the degree of susceptibility. 相似文献
89.
Ruiz A Molina JM González J Martínez-Moreno FJ Gutiérrez PN Martínez-Moreno A 《Veterinary research》2003,34(4):435-443
The use of cysteine proteinases from Fasciola hepatica adult flukes for the serodiagnosis of caprine fasciolosis by means of an indirect ELISA test was studied. Two proteolytic fractions from adult fluke homogenates, with apparent molecular weights of 28 and 34 kDa (P28 and P34 respectively), were characterised as cysteine proteinases using azocasein assays and gelatin gel analysis. Both P28 and P34 fractions were electroluted and used as antigens in two different indirect ELISA tests. Serum IgG levels against P28 and P34 in goats given an experimental primary infection with 200 metacercariae or in goats given two experimental infections with 200 metacercariae were determined and compared with those observed in an uninfected control group. ELISA tests using both cysteine proteases showed a rapid and consistent detection of specific IgG in all experimentally infected goats. The IgG response to P28 was the first to be detected as early as 2-3 weeks post-infection and remained elevated throughout the experiment. The response to P34 was detected later (4-6 wpi) and disappeared in some animals at 18 wpi, while flukes were still present in the bile ducts. No significant differences were observed between the anti-P28 and anti-P34 IgG responses between animals receiving a primary or a challenge infection. The results of our study, although preliminary, are promising since the P28 ELISA described here may be a reliable method for the immunodiagnosis of F. hepatica infection in goats. 相似文献
90.