Investigation of pseudorabies virus latency in nervous tissues of seropositive pigs exposed to field strain     

Yoon HA  Eo SK  Aleyas AG  Cha SY  Lee JH  Chae JS  Jang HK  Cho EG  Song HJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(2):143-148

The prevalence and quantity of latent pseudorabies virus (PrV) in nervous tissues of pigs exposed to field strain in Korea was investigated by nested and real-time PCR. Nervous tissues including trigeminal ganglion (TG), olfactory bulb (OB), and brain stem (BS) were collected from 94 seropositive pigs. PrV latent infection in nervous tissues was initially investigated by nested PCR targeting three glycoprotein genes (gB, gE, and gG). Based on the obtained result, latent infection was detected in 95.7% of screened animals. Furthermore, it was revealed that the examined tissues harbored different copy numbers of latent PrV genome ranging from <10(2.0) to 10(7.1) copies per microgram of genomic DNA in real-time PCR analysis. These results show that under normal conditions, levels of latent PrV in the nervous tissues of pigs can vary across a wide range. Therefore, the data presented here provides information regarding control of the endemic state of PrV in Korea.  相似文献   

Application of a solid-phase fluorescence immunoassay to determine oxytetracycline and tetracycline residues in tissue of olive flounder (Paralichthys olivaceus)     

Kim S  Chung HS  Ha JY  Jung WC  Heo SH  Lee HJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1243-1245

Parallux, a solid-phase fluorescence immunoassay (SPFIA) developed for antibotics residue detection in milk, was applied for analysis of fish tissue. The recommended therapeutic doses of oxytetracycline (OTC, 100 g/ton water, withdrawal period 30 days) and tetracycline (TC, 150 g/ton water, withdrawal period 30 days) were treated to a group of 35 olive flounders (Paralichthys olivaceus) using dipping administration. Muscle was sampled before and after drug treatment 1st, 2nd, 3rd, 5th, 7th, and 14th day. The concentration of oxytetracycline in muscle, determined by SPFIA, was compared with that of internal standard (100 ppb as oxytetracycline). The S/C ratio of sample inhibition value to cutoff inhibition value was employed as an index to determine the muscle residue in olive flounder. To investigate the recovery rate, and standard solutions were added to muscle samples to give final concentrations in muscle of 0.1 and 0.5 microg/ml. The recovery rates of all spiked samples were >89% of the spiked value. OTC and TC were detected in muscle of fishes treated until the 3rd day of withdrawal period. The present study showed that the SPFIA can be easily adopted in predicting tissue residues for OTC and TC in farmed fishes.  相似文献   

Growth and Body Composition Effects of Tuna Byproduct Meal Substituted for Fish Meal in the Diet of Juvenile Abalone,Haliotis discus          下载免费PDF全文

Won‐Gwan Jung  Hee Sung Kim  Ki Wook Lee  Ye Eun Kim  Dong Kyu Choi  Bok‐Il Jang  Sung Hwoan Cho  Cheol Young Choi  Byeng‐Hak Kim  Yang‐Ick Joo 《Journal of the World Aquaculture Society》2016,47(1):74-81

The effects on growth and body composition that result from tuna byproduct meal (TBM) substituted for fish meal in the diet of juvenile abalone, Haliotis discus, were determined. One thousand two hundred sixty juvenile abalone were randomly distributed into 18 70‐L plastic rectangular containers. Six experimental diets were prepared in triplicate. The TBM0 diet included 28% fish meal and 13% soybean meal as the protein source. Twenty‐five, 50, 75, and 100% of the fish meal were substituted with TBM. Finally, salted sea tangle was prepared. The essential amino acids, such as isoleucine, lysine, and valine, tended to decrease with the dietary substitution of TBM for fish meal in the experimental diets. The weight gain and specific growth rate (SGR) of abalone that were fed the TBM25 diet were higher than those of abalone that were fed the other diets. The crude protein content of the soft body of the abalone linearly decreased with the dietary substitution of TBM for fish meal. In conclusion, as much as 75% of the fish meal in the diet of abalone can be replaced with TBM without retardation in weight gain and SGR of the abalone when 28% fish meal was included.  相似文献   

Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs     

Cho-Hee Jee  Na-Young Eom  Hyo-Mi Jang  Hae-Won Jung  Eul-Soo Choi  Jin-Hee Won  Il-Hwa Hong  Byeong-Teck Kang  Dong Wook Jeong  Dong-In Jung 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):79-87

This study was conducted to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as a method of application to acute cutaneous wounds in dogs. Healthy adult beagles (n = 3) were used in this study. Autologous PRP was separated from anticoagulant treated whole blood in three dogs. Cutaneous wounds were created and then treated by intralesional injection of PRP in the experimental group, while they were treated with saline in the control group on days 0, 2 and 4. The healing process was evaluated by gross examination throughout the experimental period and histologic examination on day 7, 14 and 21. In PRP treated wounds, the mean diameter was smaller and the wound closure rate was higher than in the control. Histological study revealed that PRP treated wounds showed more granulation formation and angiogenesis on day 7, and faster epithelialization, more granulation formation and collagen deposition were observed on day 14 than in control wounds. On day 21, collagen deposition and epithelialization were enhanced in PRP treated groups. Overall, PRP application showed beneficial effects in wound healing, and intralesional injection was useful for application of PRP and could be a good therapeutic option for wound management in dogs.  相似文献   

The fish fauna of mountain streams in South Korean national parks and its significance to conservation of regional freshwater fish biodiversity     

Min-Ho Jang  Martyn C Lucas 《Biological conservation》2003,114(1):115-126

The fish fauna of mountain streams in the Jiri (440 km²) and Seorak (373 km²) National Park areas of South Korea was investigated from September 1998 to May 2001. A total of 5979 fish were collected and classified into 22 families and 60 species from both national parks. At Jiri, 30 species in 12 families of fishes were collected, while at Seorak there were 42 species in 17 families. Zacco temmincki [relative abundance (RA) 47.9%] was dominant at both parks. Subdominant species were Rhynchocypris kumkangensis (RA 10.8%), Zacco platypus (RA 9.4%), and Pungtungia herzi (RA 5.3%). A total of 20 species were found to be Korean endemic species, representing seven families. The relative abundance of Korean endemic species was higher at Seorak (30.5%) than Jiri (18.5%). One exotic species (Oncorhynchus mykiss) and two species translocated outside their native catchments (Hypomesus nipponensis and Coreoperca herzi) were collected. The proportion of Korean endemic freshwater fish species in both parks (33.3%) was higher than the overall proportion on the Korean peninsula (23.6%). Within the 14 South Korean mountain-area national parks as a whole, larger parks tend to have higher fish diversity. We conclude that South Korean national parks are important for conservation of the regional fish fauna, especially for endemic and endangered species. Current threats to conservation of fishes within South Korean national parks are identified and management solutions are suggested.  相似文献   

Constituents of Musa x paradisiaca cultivar with the potential to induce the phase II enzyme,quinone reductase     

Jang DS  Park EJ  Hawthorne ME  Vigo JS  Graham JG  Cabieses F  Santarsiero BD  Mesecar AD  Fong HH  Mehta RG  Pezzuto JM  Kinghorn AD 《Journal of agricultural and food chemistry》2002,50(22):6330-6334

A new bicyclic diarylheptanoid, rel-(3S,4aR,10bR)-8-hydroxy-3-(4-hydroxyphenyl)-9-methoxy-4a,5,6,10b-tetrahydro-3H-naphtho[2,1-b]pyran (1), as well as four known compounds, 1,2-dihydro-1,2,3-trihydroxy-9-(4-methoxyphenyl)phenalene (2), hydroxyanigorufone (3), 2-(4-hydroxyphenyl)naphthalic anhydride (4), and 1,7-bis(4-hydroxyphenyl)hepta-4(E),6(E)-dien-3-one (5), were isolated from an ethyl acetate-soluble fraction of the methanol extract of the fruits of Musa x paradisiaca cultivar, using a bioassay based on the induction of quinone reductase (QR) in cultured Hepa1c1c7 mouse hepatoma cells to monitor chromatographic fractionation. The structure and relative stereochemistry of compound 1 were elucidated unambiguously by one- and two-dimensional NMR experiments ((1)H NMR, (13)C NMR, DEPT, COSY, HMQC, HMBC, and NOESY) and single-crystal X-ray diffraction analysis. Isolates 1-5 were evaluated for their potential cancer chemopreventive properties utilizing an in vitro assay to determine quinone reductase induction and a mouse mammary organ culture assay.  相似文献   

Differential effects of powdered whole soy milk and its hydrolysate on antiobesity and antihyperlipidemic response to high-fat treatment in C57BL/6N mice     

Choi JY  Jeon JE  Jang SY  Jeong YJ  Jeon SM  Park HJ  Choi MS 《Journal of agricultural and food chemistry》2011,59(6):2584-2591

This study was performed to investigate the beneficial effects of powdered whole soy milk and its hydrolysate, compared to the processed soy milk and its hydrolysate, on the alteration of lipid metabolism and their possible effects on antiobesity in C57BL/6N mice fed a high-fat and -cholesterol diet. The mice were divided into a control group (20% casein) and four test groups for 5 weeks: soy milk (SM, 20% soy milk protein), soy milk hydrolysate (SMH, 20% hydrolyzed soy milk protein), whole soy milk (WSM, 20% whole soy milk protein), and whole soy milk hydrolysate (WSMH, 20% whole soy milk hydrolysate protein). The body weight and adipose tissue weights were significantly lowered in SMH, WSM, and WSMH groups compared to the control group despite providing an isoenergetic diet. Plasma lipid concentrations and hepatic fatty acid synthase (FAS) and glucose-6-phosphate dehydrogenase (G6PD) activities were significantly lowered in all soy milk groups; however, the hepatic lipid contents and malic enzyme (ME) activity were only significantly lowered in the WSM and WSMH groups, compared to the control group. Data suggest that powdered WSM or WSMH appears to be more beneficial than SM or SMH in overall antiobesity and antihyperlipidemic properties following in the order WSMH/WSM, SMH, SM, and casein.  相似文献   

Preventive effect of t,t-conjugated linoleic acid on 12-O-tetradecanoylphorbol-13-acetate-induced inhibition of gap junctional intercellular communication in human mammary epithelial MCF-10A cells     

Rakib MA  Kim YS  Jang WJ  Jang JS  Kang SJ  Ha YL 《Journal of agricultural and food chemistry》2011,59(8):4164-4170

The anti-tumor promotional effects of t9,t11-conjugated linoleic acid (t9,t11-CLA) and t10,t12-CLA were evaluated on the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inhibition of gap junctional intercellular communication (GJIC) in the human mammary epithelial cell line MCF-10A. The results were compared to those obtained from c9,t11-CLA, which is a more effective anti-tumor promoter on TPA-induced GJIC inhibition in MCF-10A cells than t10,c12-CLA. Cells were treated with 20 μM t9,t11-CLA, t10,t12-CLA, or c9,t11-CLA for 24 h followed by 60 nM TPA for 1 h. Both t9,t11-CLA and t10,t12-CLA equally protected MCF-10A cells from TPA-induced inhibition of GJIC with inferior efficacy to c9,t11-CLA.The protection was due to the ameliorated phosphorylation of connexin43 via suppression of extracellular signal-regulated kinases (ERK1/2) activation. Suppression of TPA-induced reactive oxygen species (ROS) generation by t9,t11-CLA and t10,t12-CLA was less effective, relative to c9,t11-CLA. The results suggest that the anti-promotional activities of t9,t11-CLA and t10,t12-CLA are equal but less potent than c9,t11-CLA in TPA-treated MCF-10A cells. The activity might be mediated by the attenuation of ROS production in MCF-10A cells by preventing the downregulation of GJIC during the cancer promotion stage.  相似文献   

Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate     

Min-Kyoung Shin  Myung Hwan Jung  Won-Jung Lee  Pil Son Choi  Yong-Suk Jang  Han Sang Yoo 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(4):401-403

Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.  相似文献   

Identification and determination of antigenic proteins of Korean ranavirus-1 (KRV-1) using MALDI-TOF/TOF MS analysis     

Kim YR  Hikima J  Jang HB  Nho SW  Park SB  Cha IS  Ohtani M  Eom AH  Aoki T  Jung TS 《Comparative immunology, microbiology and infectious diseases》2011,34(3):237-245

Ranaviruses are serious pathogens of fish, amphibians, and reptiles, and pose a major threat to global biodiversity. A ranavirus isolated from tissues of diseased tadpoles and frogs in Gangwon province, Korea, in 2006 and 2007, was designated Korean ranavirus-1 (KRV-1) and was infectious in a variety of fish cell lines with highest titers (10¹⁰ TCID₅₀/ml) in Epithelioma papulosum cyprini cells (EPCs) and baby hamster kidney-21 (BHK-21) cells. Bullfrog (Rana catesbeiana) tadpoles challenged by immersion in 10⁵ TCID₅₀/ml of KRV-1 showed 60% mortality within 10 days. SDS-PAGE of frog virus 3 (FV3) and KRV-1 proteins yielded several bands 35-49 kDa in size, which were identified as major capsid proteins (MCPs) by MALDI-TOF MS. Immunoblotting of FV3 proteins showed antigenic bands 34 kDa and 93 kDa in size which were identified by MALDI-TOF/TOF MS as MCP and neurofilament triplet H1-like protein (NF-H1), respectively. In KRV-1, antigenic bands at 32 kDa, 69 kDa, and 72 kDa were identified as MCP, Hypothetical protein, and NF-H1, respectively. The genes encoding these KRV-1 proteins were sequenced. KRV-1 appeared to be closely related to the soft-shelled turtle iridovirus (STIV), based on alignments of amino acid sequences from various ranaviruses. Variability in ranavirus antigenic proteins was apparent in an earlier study. It is expected that use of the methods employed here, together with the results of the present work, will contribute to an understanding of the pathogenesis of ranaviruses, and will further the development of DNA- or protein-based bait vaccines for conservation of natural habitats.  相似文献