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91.
92.
棉花单核苷酸多态性标记研究进展 总被引:1,自引:0,他引:1
单核苷酸多态性标记已在农作物研究中得到广泛应用并取得重大进展。为了便利棉花SNP(Single nucleotide polymorphism)标记的研究和应用,介绍了利用基因芯片、简化基因组测序、重测序等在棉花中开发SNP标记的方法 ,综述了SNP标记在棉花遗传图谱构建、数量位点的定位和分子标记辅助育种、基因组测序以及系统进化等研究中的应用。并对异源四倍体棉花中SNP标记开发时,同源序列位点和部分同源序列位点上的SNP标记辨别问题进行了系统探讨,对其快捷的开发、检测方式和在数量基因定位中的应用前景进行了展望。 相似文献
93.
Mingshi Li Chengquan Huang Wenjuan Shen Xinyu Ren Yingying Lv Jingrui Wang Zhiliang Zhu 《林业研究》2016,27(6):1329-1341
Forest losses or gains have long been recognized as critical processes modulating the carbon flux between the biosphere and the atmosphere. Timely, accurate and spatially explicit information on forest disturbance and recovery history is required for assessing the effectiveness of existing forest management. The major objectives of our research focused on testing the mapping efficacy of the vegetation change tracker (VCT) model over a forested area in China. We used a new version of VCT algorithm built upon the Landsat time series stacks (LTSS). The LTSS consisted of yearly image acquisitions to map forest disturbance history from 1987 to 2011 over the Ning-Zhen Mountains, Jiangsu Province of east China. The LTSS consisted of TM and ETM+ scenes with different projections due to distinct data sources (Beijing remote sensing ground station and the USGS EROS Center). The validation results of the disturbance year maps showed that most spatial agreement measures ranged from 70 to 86 %, comparable with the VCT accuracies reported for many places in USA. Very low accuracies were identified in 1995 (38.3 %) and 1992 (56.2 %) in the current analysis. These resulted from the insensitivity of the VCT algorithm to detect low intensity disturbances and also from the mis-registration errors of the image pairs. Major forest disturbance types existing in our study area were identified as agricultural expansion (39.8 %), urbanization (24.9 %), forest management practice (19.3 %), and mining (12.8 %). In general, there was a gradual decreasing trend in forest cover throughout this region, caused principally by China’s economic, demographic, environmental and political policies and decisions, as well as some weather events. While VCT has largely been used to assess long term changes and trends in the USA, it has great potential for assessing landscape level change elsewhere throughout the world. 相似文献
94.
The effects of ammonia and nitrite on vigour, survival rate, moulting rate of zoea of blue swimming crab, Portunus pelagicus, were studied. A total of five nitrite-N treatments (26.67, 53.34, 106.68, 213.36, 426.72 mg/l) and a control (no nitrite-N added) were
set up for the acute nitrite-N toxicity experiment; a total of five ammonia-N treatments (8.43, 16.86, 33.72, 67.44, 134.88 mg/l)
and a control (no ammonia-N added) were set up for the acute ammonia-N toxicity experiment. The results showed that the vigour,
survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 53.34 mg/l were significantly different
(P < 0.05) from the control group. The zoea LC50 values (mg/l) of nitrite-N were 179.47, 76.56, 66.70, 37.49, 25.01, 25.35, 25.34 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively.
The vigour, survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 16.86 mg/l were significantly
different (P < 0.05) from the control group. The zoea LC50 values (mg/l) of ammonia-N were 51.04, 39.62, 38.72, 24.43, 16.90, 13.42, 11.16 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively.
The zoeae are highly sensitive to ammonia and nitrite, and the toxicity of ammonia and nitrite on Portunus pelagicus decrease with development of this crab. 相似文献
95.
96.
目的 检验中文版沟通技能态度量表(the Chinese version of Communication Skills Attitude Scale,Ch-CSAS)的信度和效度.方法 采用委员会法,经前译、回译与跨文化调整形成Ch-CSAS,预试验后用于267名本科护理专业学生中,以检验其信度和效度.结果 Ch-CSAS为双因子结构,分别称为正向态度和负向态度.各因子及总表的克朗巴赫系数(α)分别为0.799、0.723、0.841,重测信度系数为0.894.结论 Ch-CSAS具有较好的测评属性,可用于对护理专业学生或护士的沟通技能态度的评价. 相似文献
97.
Haibo Wen Ruobo Gu Zheming Cao Xin Zhou Zhijuan Nie Xianping Ge Pao Xu Xin Zhou Zhijuan Nie Xianping Ge Pao Xu Dan Hua 《Journal of the World Aquaculture Society》2013,44(1):154-160
The variation and inheritance of juvenile shell color and pigmentation pattern in the freshwater mussel, Hyriopsis cumingii, were reported in 1‐yr‐old progeny of eight families. There were three distinctive phenotypes of shell color and pigmentation patterns observed, including a greenish‐brown shell with radial rays, yellowish‐brown shell with radial rays, and yellowish‐brown shell without radial rays. There were no greenish‐brown individuals without radial rays. The shell color phenotypes showed variation with the growth in juvenile Stage I (1–3 cm in shell length), and the percentage of individuals with radial rays increased once they reached a shell length of 11 mm and then stabilized after reaching 20 mm in shell length. Shell color differentiation became more apparent at a shell length of 26 mm. Results of chi‐square tests of the segregation ratio of shell color or ray phenotypes obtained from eight families at juvenile Stage II (6–9 cm in shell length) suggested that greenish‐brown is controlled by a dominant allele (G) and yellowish‐brown‐shell phenotype is by a recessive allele (y); the ray pattern phenotype is controlled by a recessive (r) and a dominant allele (R) at a single locus. Shell color phenotypes may be a useful genetic marker for future selective breeding of triangle pearl mussels. 相似文献
98.
干旱是影响宁夏东部沙化区荒漠草地退化的主要自然因素,也是天然草地补播成功率低的主要原因,该地区大面积种植的柠条是土壤覆盖保水的丰富资源,但是缺乏相关研究。选择平坦的、未生长柠条的退化天然补播草地作为研究对象,在临近有柠条生长的草地平茬其柠条枝条用于试验覆盖,研究不同柠条覆盖厚度(TSM0,覆盖0 cm;TSM1,覆盖1 cm;TSM2,覆盖2 cm;TSM3,覆盖3 cm)对该地区荒漠草地0~40 cm土壤水热以及补播牧草地上生物量的影响。结果表明,柠条枝条覆盖显著提高了土壤含水量,并且随着覆盖厚度的增加逐渐增大,但2018和2019年间所有覆盖处理下0~20 cm土壤水分平均值与TSM0之间差异不显著(P>0.05);在20~40 cm土层,3种柠条枝条覆盖厚度明显较对照提高了土壤水分含量,表现出TSM3>TSM2>TSM1>TSM0的趋势。结果还表明,柠条枝条覆盖还具有降低草地0~10 cm温度的作用,生长季8:00、14:00和18:00的土壤温度总体均随着柠条枝条覆盖厚度的增加出现降低趋势,且2019年土壤温度随着覆盖厚度的增加,变异系数也在减小,依次为4.6%、2.3%、1.7%,表明覆盖对土壤温度的变化具有缓冲作用。补播牧草地上生物量亦随着覆盖厚度的增加而增大,蒙古冰草、沙打旺及补播的3种牧草的总生物量均与草地20~40 cm土壤水分呈显著正相关(P<0.05)。本研究表明,柠条枝条覆盖有效地改善了荒漠草地土壤的水热,提高了土壤墒情,为牧草种植提供了良好的生长环境,可为该地区及类似地区退化草地的补播改良提供技术借鉴。 相似文献
99.
自2018年非洲猪瘟(African swine fever,ASF)传入中国以来疫情得到了有效控制。但2021年以后,我国的ASF流行情况出现了新的变化,临床中出现了“自然变异株”。这些毒株可能导致感染后临床症状不典型,容易与其他疫病混淆等问题。这与国外的流行演变规律一致,即当ASF在一个国家或地区流行较长时间后,其临床表现将由急性发病转变为缓慢发病或出现新的临床表现。经过多年的观察积累,国外已经对该病毒的流行规律特别是自然弱毒株的演变进程有了大量记载,但国内目前尚无这方面的描述。为此,就ASF自然弱毒株的演变进程进行综述,从而提出加强血清学诊断产品研发、加快产业化报批进程、适时开展血清学监测追溯等建议,以期为我国ASF科学防控提供参考。 相似文献
100.