应用RACE技术扩增驴DGAT1基因3′端及序列分析     

肖海霞  托乎提·阿及德  田可川  刘明军  陈从英 《中国奶牛》2011,(20):1-5

DGAT1（脂酰辅酶A：二脂酰甘油酰基转移酶）基因是产奶性状的一个重要功能侯选基因。由于通过预测奶牛DGAT1基因序列与马属动物DGAT1基因序列具有98%的同源性,本试验从影响牛产奶性状的DGAT1基因出发,以驴乳腺组织的RNA为模板,按照不同物种DGAT1基因的相似性设计特异引物,运用PCR和3′RACE技术扩增并获得了特异片断,特异片段回收纯化连接到pUCmT Vector载体后,转化到大肠杆菌中并筛选阳性菌落;提取质粒进行测序,结果发现该段序列与预期的目标一致,通过与其他动物同源性比较分析说明已首次克隆到驴DGAT1基因3′端。  相似文献   

托木尔峰自然保护区地衣的补充研究   总被引：6，自引：0，他引：6  

艾尼瓦尔·铁木尔  阿不都拉·阿巴斯 《干旱区研究》2000,17(3):20-27

我们根据多年的研究并结合前人的工作进一步地整理了分布在新疆托木尔峰自然保护区的地衣种类。分析该地区的地衣种有１２０个，分别隶性于４９属，其中有１１个变种和４个变型。调查中我们还发现该地区地衣随着海拔高度的升高而有所不同，北坡和南坡也有明显的差异。  相似文献   

Quantitation of transgenic plant DNA in leachate water: real-time polymerase chain reaction analysis     

Gulden RH  Lerat S  Hart MM  Powell JR  Trevors JT  Pauls KP  Klironomos JN  Swanton CJ 《Journal of agricultural and food chemistry》2005,53(15):5858-5865

Roundup Ready (RR) genetically modified (GM) corn and soybean comprise a large portion of the annual planted acreage of GM crops. Plant growth and subsequent plant decomposition introduce the recombinant DNA (rDNA) into the soil environment, where its fate has not been completely researched. Little is known of the temporal and spatial distribution of plant-derived rDNA in the soil environment and in situ transport of plant DNA by leachate water has not been studied before. The objectives of this study were to determine whether sufficient quantities of plant rDNA were released by roots during growth and early decomposition to be detected in water collected after percolating through a soil profile and to determine the influence of temperature on DNA persistence in the leachate water. Individual plants of RR corn and RR soybean were grown in modified cylinders in a growth room, and the cylinders were flushed with rain water weekly. Immediately after collection, the leachate was subjected to DNA purification followed by rDNA quantification using real-time Polymerase Chain Reaction (PCR) analysis. To test the effects of temperature on plant DNA persistence in leachate water, water samples were spiked with known quantities of RR soybean or RR corn genomic DNA and DNA persistence was examined at 5, 15, and 25 degrees C. Differences in the amounts and temporal distributions of root-derived rDNA were observed between corn and soybean plants. The results suggest that rainfall events may distribute plant DNA throughout the soil and into leachate water. Half-lives of plant DNA in leachate water ranged from 1.2 to 26.7 h, and persistence was greater at colder temperatures (5 and 15 degrees C).  相似文献   

芜菁种子中总黄酮提取工艺研究   总被引：2，自引：0，他引：2  

毛居代  帕热德姑丽·托合提  布海力期木·伊米提 《种子》2011,30(1)

采用乙醇浸提法提取芜菁种子中的黄酮类化合物。结果表明:浸提提取的最佳条件是50 ml 70%乙醇在70℃下提取2 h。本试验采用分光光度法,以芦丁标准品测定芜菁种子提取物中总黄酮的含量,总黄酮含量为2.01%.方法简便,快速,重现性好,可作为检测芜菁种子提取物中黄酮含量的一种手段。  相似文献   

Apollo 14 and apollo 16 heavy-particle dosimetry experiments     

Fleischer RL  Hart HR  Comstock GM  Carter M  Renshaw A  Hardy A 《Science (New York, N.Y.)》1973,181(4098):436-438

Doses of heavy particles at positions inside the command modules of Apollo missions 8, 12, 14, and 16 correlate well with the calculated effects of solar modulation of the primary cosmic radiation. Differences in doses at different stowage positions indicate that the redistribution of mass within the spacecraft could enhance safety from the biological damage that would otherwise be expected on manned, deep-space missions.  相似文献   

Mycobacterium tuberculosis in macrophages: effect of certain surfactants and other membrane-active compounds   总被引：10，自引：0，他引：10  

P D Hart 《Science (New York, N.Y.)》1968,162(854):686-689

Some compounds, not directly inhibitory or enhancing, nevertheless influence growth of tubercle bacilli in macrophages in cell culture. They include certain surfactants whose effects can be varied by their structural design. The compounds are probably stored in cell lysosomes. They can interact with various membranes to affect permeability. The anti- and protuberculous surfactants differ in such interaction and also in effect on lysosomal enzyme activity in infected macrophages. A link between the effect on lysosomal membranes and on tuberculous infection is suggested.  相似文献   

Frame-shift deletions in patients with Duchenne and Becker muscular dystrophy   总被引：33，自引：0，他引：33  

S B Malhotra  K A Hart  H J Klamut  N S Thomas  S E Bodrug  A H Burghes  M Bobrow  P S Harper  M W Thompson  P N Ray 《Science (New York, N.Y.)》1988,242(4879):755-759

Duchenne muscular dystrophy (DMD) and its less severe form Becker muscular dystrophy (BMD) are allelic disorders. It has been suggested that in the mutations involving BMD, the translational reading frame of messenger RNA is maintained and a smaller, though partially functional, protein is produced. In order to test this, the exon-intron boundaries of the first ten exons of the DMD gene were determined, and 29 patients were analyzed. In a number of BMD patients (mild and severe BMD), the reading frame of messenger RNA was not maintained. On the basis of these findings, a model for reinitiation from an internal start codon is suggested.  相似文献   

化肥污染的防治措施和方法探讨   总被引：2，自引：0，他引：2  

哈丽丹·买买提  阿不利米提 《新疆农业科学》2005,42(Z1):136-138

滥用化肥或施肥不当不仅会使土壤、植物养分失调,而且对农作物产量及品质构成威胁,更为严重的是未被植物吸收的化肥残留,对土壤、地表水、地下水造成污染。因此,在肯定施用化肥对农业增产有重要作用的同时,探讨预防化肥污染的方法和措施是十分必要的。  相似文献   

Particle Track, X-ray, Thermal, and Mass Spectrometric Studies of Lunar Material     

Fleischer RL  Haines EL  Hanneman RE  Hart HR  Kasper JS  Lifshin E  Woods RT  Price PB 《Science (New York, N.Y.)》1970,167(3918):568-571

Particle tracks in Apollo 11 samples are dominantly of cosmic ray and solar origin: primary galactic and solar flare particles, likely spallation recoil tracks, and possible solar-wind heavy particles. The energy spectrum of irongroup nuclei is inferred from track density gradients in surface layers, and a limit of < 10(-7) centimeter per year is deduced for the surface erosion rate. From cosmic ray tracks in rock and core samples it is clear that the lunar soil is stirred often during each few million years. X-rays reveal augite, anorthite, olivine, ilmenite, troilite, nonmeteoritic iron, and assorted glasses, but no major structural damage. Hydrogen, helium, and other gases in the fines are compatible with expected solar wind ratios.  相似文献   

Condensation nucleus discriminator making optical measurements on fog: a tool for environmental research     

Hart EJ  Schmidt KH  Vasudevan KN 《Science (New York, N.Y.)》1973,180(4090):1064-1067

An instrument providing a new, rapid, and accurate method of determining the number and critical radii of condensation nuclei with radii under 200 angstroms is described. Based on the principle of the cloud chamber, the instrument measures transient changes in the attenuation and scattering of a monochromatic light beam by the growing fog droplets. From data obtained the absolute number concentration and radii of condensation nuclei can be calculated. Preliminary studies of aerosol formation in beta-irradiated mixtures of air and sulfur dioxide showed that carbon monoxide and methane inhibit the formation of nuclei; relative rate constants can be deduced. Some applications of this instrument for environmental and basic research are pointed out.  相似文献