Comparison of the in vitro effects of saline,hypertonic hydroxyethyl starch,hypertonic saline,and two forms of hydroxyethyl starch on whole blood coagulation and platelet function in dogs          下载免费PDF全文

Virginie A. Wurlod Dr. vet. med.  Judith Howard Dr. vet. med.  DACVIM  Thierry Francey Dr. vet. med.  DACVIM  Ariane Schweighauser Dr. vet. med.  DACVIM  Katja N. Adamik Dr. vet. med.  DACVECC 《Journal of Veterinary Emergency and Critical Care》2015,25(4):474-487

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Spectrophotometric determination of yellow pigment content and evaluation of carotenoids by high-performance liquid chromatography in durum wheat grain     

Hentschel V  Kranl K  Hollmann J  Lindhauer MG  Böhm V  Bitsch R 《Journal of agricultural and food chemistry》2002,50(23):6663-6668

The so-called "yellow pigment" content of durum wheat has been used for a long time as an indicator of the color quality of durum wheat and pasta products. For decades the chemical nature of these pigments has been assigned to carotenoids, mainly to the xanthophyll lutein and its fatty acid esters. The chemical composition of the yellow pigments of eight German durum wheat cultivars was studied. Grains were milled on a laboratory mill. Pigment extraction of millstream fractions was performed according to the optimized ICC standard method 152 procedure, and the chemical composition of the extract was analyzed by isocratic reversed phase high-performance liquid chromatography. all-trans-Lutein ranged from 1.5 to 4 mg kg(-1), and zeaxanthin was found in traces. No lutein esters and carotenes were detected. Surprisingly, the fraction of carotenoids of the complete yellow pigment content amounted to only 30-50% of the yellow pigment quantities, so there are still compounds in durum wheat not yet identified that contribute considerably to the yellow color of the grain extracts. The isolation and chemical identification of those pigments are under investigation.  相似文献   

Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results     

Katja Hglund  Hanna Palmqvist  Sara Ringmark  Anna Svensson 《Journal of veterinary diagnostic investigation》2022,34(1):28

Catecholamine release increases in dogs with pheochromocytomas and in situations of stress. Although plasma catecholamines degrade rapidly, their metabolites, normetanephrine (NME) and metanephrine (ME), are stable in acidified urine. Our aim was to verify a human urine ELISA kit for the quantification of NME and ME in canine urine and to determine the effects on metabolite stability of sampling time (morning or midday) and day (ordinary or day spent in a clinic). We analyzed 179 urine samples from 17 healthy dogs. For NME, the mean intra-assay CV was 6.0% for all samples and 4.3% for the canine control; inter-assay CVs were 3.3, 3.8, and 12% for high and low concentration human urine positive controls supplied in the ELISA kit and a positive canine control, respectively; spike-recovery was 90–101%. For ME, mean intra-assay CV was 6.5% for samples and 9.0% for the canine control; inter-assay CVs were 12.7, 7.2, and 22.5% for high and low concentration human urine positive controls supplied in the ELISA kit and a positive canine control, respectively; spike-recovery was 85–89%. Dilution recovery was unsatisfactory for both metabolites. Based on our verification results, NME was selected for remaining analyses. We found no effect on NME concentrations of acidification or room temperature storage for up to 24 h. The NME:creatinine ratio was higher after the first of 3 clinic days compared to the same morning (111.2 ± 5.5 vs. 82.9 ± 5.3; p < 0.0001), but not on the other days. NME verification results were generally superior to ME. Dilution studies were unsatisfactory for both metabolites. Given that NME was stable without acidification at room temperature, urine samples can be collected at home. The clinic environment can cause higher NME:creatinine ratios, especially in unaccustomed dogs.  相似文献   

Status quo of adoption of precision agriculture enabling technologies in Swiss plant production     

Groher  Tanja  Heitkämper  Katja  Walter  Achim  Liebisch  Frank  Umstätter  Christina 《Precision Agriculture》2020,21(6):1327-1350

Precision Agriculture - This paper presents the state of application of Precision Agricultural enabling Technology (PAT) in Swiss farms as an example for small-scale, highly mechanised Central...  相似文献   

What is your diagnosis? Os clitoris or os penis with ambiguous genitalia     

Wucherer K  Lulich JP 《Journal of the American Veterinary Medical Association》2006,229(9):1383-1384

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Assessment of collagen genes involved in fragmented medial coronoid process development in Labrador Retrievers as determined by affected sibling-pair analysis     

Salg KG  Temwitchitr J  Imholz S  Hazewinkel HA  Leegwater PA 《American journal of veterinary research》2006,67(10):1713-1718

OBJECTIVE: To evaluate the involvement of various collagen genes in the development of fragmented medial coronoid process (FCP) in Labrador Retrievers. SAMPLE POPULATION: 93 dogs originating from 13 litters were used in the study; FCP was diagnosed in 35 dogs, and each affected dog had at least 1 sibling that was also affected. Twelve dams and sires were included in the analysis. All dogs were purebred Labrador Retrievers except for 2 litters (offspring of a female Golden Retriever-Labrador Retriever mixed-breed dog). PROCEDURES: For each dog, DNA was isolated from blood samples. Polymorphic microsatellite markers adjacent to 14 candidate genes (ie, COL1A1, COL1A2, COL2A1, COL3A1, COL5A1, COL5A2, COL6A3, COL9A1, COL9A2, COL9A3, COL10A1, COL11A1, COL11A2, and COL24A1) were analyzed by use of PCR assays; genotypes were determined via automated detection of DNA products. The level of allele sharing between pairs of affected siblings was assessed. RESULTS: Among the 93 dogs, allele sharing of the 14 collagen genes was determined as follows: COL1A1, 45%; COL1A2, 47%; COL2A1, 37%; COL3A1, 32%; COL5A1, 43%; COL5A2, 32%; COL6A3, 36%; COL9A1, 45%; COL9A2, 49%; COL9A3, 38%; COL10A1, 46%; COL11A1, 52%; COL11A2, 47%; and COL24A1, 47%. CONCLUSIONS AND CLINICAL RELEVANCE: Because siblings share 50% of their genome at random, the fact that the percentages of allele sharing among the analyzed collagen genes were not significantly > 50% indicates that these genes are not determinant candidates for FCP in Labrador Retrievers. The gene for the vitamin D receptor could also be excluded because of its proximity to COL2A1.  相似文献   

Plant lipid composition is not affected by short‐term isotopic (13C) pulse‐labelling experiments     

Guido L. B. Wiesenberg  Katja Schneckenberger  Yakov Kuzyakov  Lorenz Schwark 《植物养料与土壤学杂志》2009,172(3):445-453

Pulse‐labelling of plants in a ¹⁴CO₂ or ¹³CO₂ atmosphere is a useful tool for investigating C allocation in plants and soils, revelation of C sources in soil organisms, as well as for CO₂ partitioning studies. Recently, these labelling experiments have been used to produce isotopically labelled biomass of plants or microorganisms for the investigation of C dynamics in these organisms or in soil. However, it remains unknown whether these labelling approaches may affect the composition of plant constituents that react to modifications of environmental conditions during biosynthesis. Lipids as primary biosynthesis products and main components of plant waxes are well known to react fast to environmental changes resulting in a modified lipid composition. In this study, we demonstrate that lipid composition may be only slightly affected by the labelling procedure, especially, when only short pulses (only a few hours) are applied and when the sampling does not occur immediately after the labelling. While the differences of plant lipid compositions are obviously modified not as a result of isotopic pulses, the environmental conditions of plants grown under controlled laboratory conditions have a significant effect leading to a shift of the distribution pattern of plant lipids compared to the lipid composition of plants grown under field conditions.  相似文献   

Repeated CO₂ pulse-labelling reveals an additional net gain of soil carbon during growth of spring wheat under free air carbon dioxide enrichment (FACE)     

Rainer Martens  Katja Heiduk  Andreas Pacholski  Hans-Joachim Weigel 《Soil biology & biochemistry》2009,41(12):2422-2429

Rising levels of atmospheric CO₂ have often been found to increase above and belowground biomass production of C3 plants. The additional translocation of organic matter into soils by increased root mass and exudates are supposed to possibly increase C pools in terrestrial ecosystems. Corresponding investigations were mostly conducted under more or less artificial indoor conditions with disturbed soils. To overcome these limitations, we conducted a ¹⁴CO₂ pulse-labelling experiment within the German FACE project to elucidate the role of an arable crop system in carbon sequestration under elevated CO₂. We cultivated spring wheat cv. “Minaret” with usual fertilisation and ample water supply in stainless steel cylinders forced into the soil of a control and a FACE plot. Between stem elongation and beginning of ripening the plants were repeatedly pulse-labelled with ¹⁴CO₂ in the field. Soil born total CO₂ and ¹⁴CO₂ was monitored daily till harvest. Thereafter, the distribution of ¹⁴C was analysed in all plant parts, soil, soil mineral fractions and soil microbial biomass. Due to the small number of grown wheat plants (40) in each ring and the inherent low statistical power, no significant above and belowground growth effect of elevated CO₂ was detected at harvest. But in comparison to ambient conditions, 28% more ¹⁴CO₂ and 12% more total CO₂ was evolved from soil under elevated CO₂ (550 μmol CO₂ mol⁻¹). In the root-free soil 27% more residual ¹⁴C was found in the FACE soil than in the soil from the ambient ring. In soil samples from both treatments about 80% of residual ¹⁴C was found in the clay fraction and 7% in the silt fraction. Very low ¹⁴C contents in the CFE extracts of microbial biomass in the soil from both CO₂ treatments did not allow assessing their influence on this parameter. Since the calculated specific radioactivity of soil born ¹⁴CO₂ gave no indication of an accelerated priming effect in the FACE soil, we conclude that wheat plants grown under elevated CO₂ can contribute to an additional net carbon gain in soils.  相似文献   

Analysing the diets of invertebrate predators using terminal restriction fragments     

Anita Juen  Katja Hogendoorn  Gang Ma  Otto Schmidt  Michael A. Keller 《Journal of pest science》2012,85(1):89-100

Analysing food webs in agricultural habitats is essential for the development of natural control strategies. Several molecular tools to investigate trophic interactions on a species-specific level have been developed in recent years and their advantages and limitations have been discussed. With this study we introduce another tool, terminal restriction fragment length polymorphism (tRFLP). Generalist predators found in Australian brassica crops, their prey and abundant parasitoids were chosen as a model system to adapt and evaluate the tRFLP approach. Using general primers and selecting six restriction enzymes, we obtained species-specific tRF patterns for 21 of the most abundant arthropods in brassica crops. We detected up to three prey species in the gut contents of laboratory-fed predators. Detection rates differed among predators, ranging between 28 and 100%. The identification of a species-specific tRF pattern was strongly affected by the presence and concentrations of DNA from other species. In a preliminary field study, prey could be identified from 20% of the collected brown lacewings. The advantage of the tRFLP method is the possibility of identifying multiple species at once. This advantage is counterbalanced by methodological limitations, among which the most critical one is the fact that it will be difficult to maintain specificity in highly diverse ecosystems. However, if species diversity is limited and the target sequence and the primers are chosen to increase detection success, the tRFLP method can be used to study trophic interactions in the field.  相似文献   

Evaluation of D‐dimer levels in aqueous humor of rabbit eyes with and without induced intraocular fibrin and fibrinolytic treatment     

Katrin Voelter  Christoph Tappeiner  Barbara Riond  Katja Nuss  Deborah Bruetsch  Simon A. Pot 《Veterinary ophthalmology》2020,23(2):212-218

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