Portal vein aneurysm in a dog     

Miyawaki S  Washizu M  Maeda S  Shibata S  Watanabe K  Yamazoe K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(9):1191-1193

Portal vein aneurysm (PVA) is a rare abnormal dilatation of the portal vein, which has not been reported in dogs. We describe the findings of ultrasound and computed tomography in a case of PVA in a young male toy poodle, with the final diagnosis established by explorative surgical observation. The dog had an aneurysmal fusiform dilatation in the extrahepatic portal vein with portal hypertension and multiple portsystemic shunts. This is the first report of canine PVA.  相似文献   

Valdensia leaf blight of highbush blueberry caused by Valdensinia heterodoxa, a new fungal disease in Japan     

Syuuichi Nekoduka  Satoko Kanematsu  Kazuaki Tanaka  Yukio Harada  Teruo Sano 《Journal of General Plant Pathology》2012,78(3):151-159

In July 2003, a new disease occurred on leaves of highbush blueberry (Vaccinium corymbossum L.) in Iwate, Japan. Leaves initially had brownish spots, which subsequently developed into large lesions with concentric rings, resulting in premature defoliation. Teardrop-shaped conidia infecting leaves were visible by the naked eye as small protuberances in the center of lesions. Star-shaped conidia were sporadically produced on large lesions. The causal fungus was identified as Valdensinia heterodoxa, based on cultural, morphological and genetic studies. Inoculation tests showed that the fungus reproduced lesions on detached young leaves of highbush blueberry. A field survey in 2009 indicated that symptoms initially appeared on the lower leaves of basal shoots in late May, and the disease rapidly progressed on leaves of basal shoots, eventually spreading to lateral shoots from late June to late July. Removal of all basal shoots in late June significantly reduced disease incidence on lateral shoots in late July.  相似文献   

Black leaf spot of Japanese persimmon (<Emphasis Type="Italic">Diospyros kaki</Emphasis>), a new disease caused by <Emphasis Type="Italic">Adisciso kaki</Emphasis> sp. nov.     

Jun?Yamamoto  Kazuaki?Tanaka  Nobuaki?Ohtaka  Toyozo?SatoEmail author 《Journal of General Plant Pathology》2012,78(2):99-105

A new defoliating disease with severe leaf spot of Japanese persimmon (Diospyros kaki) was found in Shimane Prefecture. A fungus, isolated from the ascospores that had formed on the leaf spot, was demonstrated to reproduce the symptoms on leaves and described as a new species, Adisciso kaki, based on morphological and molecular phylogenetic analyses. This fungus is characterized by small ascomata without clypeus, obclavate to broadly cylindrical asci with an amyloid apical apparatus, and hyaline ascospores with a submedian septum. We coined the name black leaf spot (Kurohoshi-rakuyo-byo, in Japanese) for the new disease.  相似文献   

Successful use of an acceleration rate response pacemaker with a transvenous steroid-eluting screw-in lead for third-degree atrioventricular block in a labrador retriever     

Miura N  Fujiki M  Misumi K  Setoyama K  Takegawa K  Takahashi T  Miyahara K  Sakamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1101-1105

Permanent pacemakers are commonly used in veterinary practice and can have a dramatic effect on the treatment of heart block. A Labrador Retriever dog suffering from exercise intolerance secondary to third degree atrioventricular block was treated with a new pacemaker system. A steroid-eluting screw-in type lead that has the advantage of being more fixed to the myocardial wall without increasing the pacing threshold was used. The heart rate was regulated with an acceleration sensing pacemaker generator that included several automatic modulation systems. Nineteen months after implantation, the dog has a normal level of activity. The present case suggests that this pacemaker design may offer important advantages for canine patients.  相似文献   

Presumptive primary cardiac lymphoma in a cat causing pericardial effusion     

Nako Shinohara DVM  John M. MacGregor DVM  Anthony Calo DVM  John E. Rush DVM  MS  Dipl ACVIM  Dipl ACVECC  Dominique G. Penninck DVM  Dipl ACVR  Joyce S. Knoll DVM  PhD  Dipl ACVP 《Journal of Veterinary Cardiology》2005,7(1):65-69

We report a case of primary cardiac lymphoma in a cat, causing pericardial effusion. A 13-year-old castrated male Himalayan cat was evaluated for chronic weight loss and radiographic finding of cardiomegaly. Pericardial effusion and a heart mass were detected via echocardiography. Pericardiocentesis and ultrasound-guided fine needle aspirate of the heart mass were performed under sedation. Antemortem diagnosis of cardiac lymphoma was made based on cytology of pericardial fluid. Based on physical examination, laboratory tests and abdominal radiographs, primary cardiac lymphoma was established as the presumptive clinical diagnosis. Treatment with chemotherapeutic agents was initiated.  相似文献   

High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system   总被引：9，自引：0，他引：9  

Nagata T  Ishikawa S  Shimokawa E  Kamikawa M  Kikuma R  Muneta Y  Yokomizo Y  Nakamura M  Takehara K 《Veterinary immunology and immunopathology》2002,87(1-2):65-72

Bioactive recombinant bovine interleukin-18 (rboIL-18) was expressed using a baculovirus system. Normally, IL-18 is translated as a precursor form of a 24kDa polypeptide and processed by IL-1beta converting enzyme (ICE) to a mature bioactive form of 18kDa protein. Hence, to express active form IL-18, we constructed two recombinant baculoviruses containing boIL-18 and human ICE (hICE) genes, respectively, and superinfected these viruses into insect cells. Superinfection of both recombinant viruses into the cells resulted in the expression of a 24kDa precursor form and an 18kDa mature form detectable in the supernatant by immunoblotting using anti-porcine IL-18 antibody. Culture supernatant from the superinfected cells showed a synergistic effect with recombinant boIL-12 for production of interferon-gamma (IFN-gamma) in bovine peripheral mononuclear cells. By addition of histidine hexamer at the C-terminal of boIL-18, the mature IL-18 was purified. Bioactivity remained after purification.  相似文献   

Production and in vivo testing of a recombinant bovine IL-12 as an adjuvant for Salmonella typhimurium vaccination in calves     

Takehara K  Kikuma R  Ishikawa S  Kamikawa M  Nagata T  Yokomizo Y  Nakamura M 《Veterinary immunology and immunopathology》2002,86(1-2):23-30

A recombinant bovine interleukin-12 (boIL-12) that contains a histidine hexamer, rboIL-12His, was produced, purified and administered to calves. We first tried the purification of heterodimer IL-12 from a mixture of p40 homodimer, p40 monomer, and p40-p35 heterodimer with a p35 subunit tagged with a histidine hexamar at its C-terminal (p35His). A recombinant baculovirus expressing p35His was generated and used for superinfection with a recombinant baculovirus expressing p40 subunit. The expressed subunits, p40 and p35His, were assembled into a 70kDa heterodimer in insect cells, released into culture medium, and then purified using a nickel chelate column. The purified rboIL-12His was bioactive for induction of IFN-gamma in bovine peripheral blood mononuclear cells (PBMCs) in vitro.The purified rboIL-12His was then administered to calves with inactivated Salmonella Typhimurium (ST). When sera were assayed by ELISA, specific anti-ST IgG1 antibodies were detected in all ST immunized calves, but, specific anti-ST IgG2 antibodies were detected only in calves administered ST along with rboIL-12His, indicating a possible switch to a Th1 response. Administration of commercially available Salmonella vaccine did not elicit IgG2 antibodies in calves. These results suggest that co-administration of IL-12 with inactivated ST cells could induce a Th1-type response in calves.  相似文献   

Surveillance of avian influenza viruses in Northern pintails (Anas acuta) in Tohoku District, Japan     

Jahangir A  Watanabe Y  Chinen O  Yamazaki S  Sakai K  Okamura M  Nakamura M  Takehara K 《Avian diseases》2008,52(1):49-53

Among winter migratory waterfowl, Northern pintails (Anas acuta), in one of the largest flocks in Tohoku district, northeast Japan, were surveyed for influenza A viruses at five wintering sites in three prefectures, viz., Aomori, Akita, and Miyagi. A total of 38 influenza A viruses were isolated from 2066 fecal samples collected during November 2006 through March 2007. The overall isolation rate was 1.84%. Eleven different subtypes were isolated, including nine H5N2, seven H6N8, seven H10N1, four H4N6, three H6N1, three H11N9, and one each of H1N1, H6N2, H6N5, H10N9, H11N1. Only the H4N6 subtype was detected during two successive months, November and December, from Lake Ogawara of Aomori prefecture. One wintering site, Lake Izunuma of Miyagi prefecture, was negative for virus isolation throughout the study period. During the sampling period, the highest virus isolation rate was in December (4.90%) followed by November (2.18%), January (0.91%), and February (0.30%). Virus isolation was negative for samples collected in March 2007. These results suggest that influenza viruses are introduced by Northern pintail when they migrate into Japan, but the viruses are not maintained in the flocks, most likely because the birds are not breeding during the winter. We believe that this relatively large data set creates a strong foundation for future studies of avian influenza virus (AIV) prevalence, evolution, and ecology in wintering sites, along with the role of Northern pintails in the spread of AIV during their migration from northern Russia and Asia to Japan.  相似文献   

Effects of temperature on the growth of a Japanese willow (Salix gilgiana Seemen)     

Kenji Shinohara  Akinori Nagao  Shiro Okuda  Kaoru Niiyama  Toyonobu Sugawa 《Journal of Forest Research》1998,3(1):55-60

The effects of temperature on the growth characteristics of a Japanese willow (Salix gilgiana Seemen) were studied with respect to dry matter production, wood anatomy and the net photosynthetic rates of leaves. Clonal materials were grown in natural-light rooms (phytotrons) at 30°C/25°C, at 25°C/20°C or at 20°C/15°C (day/night: 12 h/12 h), respectively. The elongation rate of shoots from the beginning of June to the end of July was approximately 22 cm per week under the three different sets of conditions. The shoots reached approximately 3 m in height after 4 months of growth. Total dry matter production and dry weights of stems did not differ very much among the three sets of temperature conditions after 9 months of growth. The dry weight of leaves increased at high temperatures while that of roots decreased. The number of vessels increased significantly at 30°C/25°C and 25°C/20°C, whereas the diameters of vessels and fibers, and the double wall thickness of fibers did not vary markedly under all three temperature regimes. The net photosynthetic rate and the respiration rate in mature leaves were highest at 30°C/25°C, and both activities increased with increases in growth temperature. The increase in the photosynthetic rate was much smaller than that in the respiration rate. Individual plants had been completely adapted to the respective growth temperatures, as judged from the decrease in the photosynthetic rate after the transfer to different temperatures. Part of this work was reported at the 102nd Annual Meeting of the Japanese Forestry Society (Nagoya, Japan, 1991). This work was supported by Grants-in-Aid (Integrated Research Program for Effective Use of Biological Activities to Create New Demand) from the Ministry of Agriculture, forestry and Fisheries of Japan (BRP 97-IV-B-10).  相似文献   

Development of industrial tree plantation in the administrative region No. 10, in mindanao island, philippines: With two case studies     

Diosdado A. Paler  Takeo Shinohara  Romulo A. del Castillo  Isamu Nomura 《Journal of Forest Research》1998,3(4):193-197

The study describes the development of industrial tree plantation in a specific region in the Philippines. Two case studies using plantation companies are presented. Likewise, management prospects and problems are considered. Since industrial plantations are given emphasis in the 1980’s, plantation owners are already capable to engage in development. Although majority are small scale, their management performance is considerable. Likewise, social and economic gains are enormous. Fast growing species for pulpwood are developed under different climatic conditions at low stocking density. However, further development is limited by problems particularly product development, financial assistance, and the volatility of plantation policy. Several approaches are endorsed. The study is partially funded by the Sasagawa Research Foundation of the Japan Science Society. Sincere appreciation is extended.  相似文献