The characteristics of timber production in the administrative region No. 10, in Mindanao Island, Philippines: A case study     

Diosdado A. Paler  Takeo Shinohara  Romulo A. del Castillo  Isamu Nomura 《Journal of Forest Research》1998,3(3):139-143

The characteristics of timber production in the public forest of a specific region in the Philippines are examined and the patterns on the linkages between logging, deforestation, and socio-economic development are established and illustrated. The logging industry is fully manned and financially capable and is a major contributor to the economy. However, the industry is now shrinking due to the declining forest resource of the region. The end results eventually lead to reduced productivity and contribution. The reduction of forest resources was traced to the excessive commercial logging and agricultural conversion of forest lands. Alternatives are suggested. The assistance provided by Mr. Jose D. Malvas, Jr., Director of the Forest Management Bureau, in the gathering of the data used in the study is deeply appreciated.  相似文献   

Use of herpesvirus papio 2 as an alternative antigen in immunoblotting assay for B virus diagnosis     

Tanaka S  Mannen K  Sato H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):529-532

Herpesvirus papio 2 (HVP2), which infects baboons, is much more closely related genetically and antigenically to monkey B virus (BV) than to human herpes simplex virus 1(HSV1) and other related herpes viruses. The usefulness of HVP2 as an alternative test antigen in immunoblotting assays to detect BV-antibody in macaque monkey sera was assessed. Six HVP2 proteins reacted with BV-positive sera in immunoblotting. No specific bands could be detected with BV-negative sera. These results show the usefulness of HVP2 antigen as an alternative and safer antigen than authentic BV antigen in detecting BV antibody in immunoblotting.  相似文献   

Gel-filtration analysis to detect Al-binding ligands in Eucalyptus camaldulensis     

Ko Tahara  Katsumi Kojima  Kenji Shinohara 《Journal of Forest Research》2015,20(4):411-414

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Transgenic Pigs with Pancreas-specific Expression of Green Fluorescent Protein     

Hitomi MATSUNARI  Toshihiro KOBAYASHI  Masahito WATANABE  Kazuhiro UMEYAMA  Kazuaki NAKANO  Takahiro KANAI  Taisuke MATSUDA  Masaki NAGAYA  Manami HARA  Hiromitsu NAKAUCHI  Hiroshi NAGASHIMA 《The Journal of reproduction and development》2014,60(3):230-237

The development and regeneration of the pancreas is of considerable interest because of the role of these processes in pancreatic diseases, such as diabetes. Here, we sought to develop a large animal model in which the pancreatic cell lineage could be tracked. The pancreatic and duodenal homeobox-1 (Pdx1) gene promoter was conjugated to Venus, a green fluorescent protein, and introduced into 370 in vitro-matured porcine oocytes by intracytoplasmic sperm injection-mediated gene transfer. These oocytes were transferred into four recipient gilts, all of which became pregnant. Three gilts were sacrificed at 47–65 days of gestation, and the fourth was allowed to farrow. Seven of 16 fetuses obtained were transgenic (Tg) and exhibited pancreas-specific green fluorescence. The fourth recipient gilt produced a litter of six piglets, two of which were Tg. The founder Tg offspring matured normally and produced healthy first-generation (G1) progeny. A postweaning autopsy of four 27-day-old G1 Tg piglets confirmed the pancreas-specific Venus expression. Immunostaining of the pancreatic tissue indicated the transgene was expressed in β-cells. Pancreatic islets from Tg pigs were transplanted under the renal capsules of NOD/SCID mice and expressed fluorescence up to one month after transplantation. Tg G1 pigs developed normally and had blood glucose levels within the normal range. Insulin levels before and after sexual maturity were within normal ranges, as were other blood biochemistry parameters, indicating that pancreatic function was normal. We conclude that Pdx1-Venus Tg pigs represent a large animal model suitable for research on pancreatic development/regeneration and diabetes.  相似文献   

Analysis of the Mechanism of Reactivation of Latently Infecting Pseudorabies Virus by Acetylcholine     

Seiichi TANAKA  Kazuaki MANNEN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):719-722

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Element-selective single atom imaging     

Suenaga K  Tence M  Mory C  Colliex C  Kato H  Okazaki T  Shinohara H  Hirahara K  Bandow S  Iijima S 《Science (New York, N.Y.)》2000,290(5500):2280-2282

Electron energy-loss spectroscopy (EELS) is widely used to identify elemental compositions of materials studied by microscopy. We demonstrate that the sensitivity and spatial resolution of EELS can be extended to the single-atom limit. A chemical map for gadolinium (Gd) clearly reveals the distribution of Gd atoms inside a single chain of metallofullerene molecules (Gd@C82) generated within a single-wall carbon nanotube. This characterization technique thus provides the "eyes" to see and identify individual atoms in nanostructures. It is likely to find broad application in nanoscale science and technology research.  相似文献   

Chemical studies on antioxidant mechanism of curcuminoid: analysis of radical reaction products from curcumin   总被引：10，自引：0，他引：10  

Masuda T  Hidaka K  Shinohara A  Maekawa T  Takeda Y  Yamaguchi H 《Journal of agricultural and food chemistry》1999,47(1):71-77

In the course of studies on the antioxidant mechanism of curcumin, its radical reaction was investigated. Curcumin was reacted with radical species, which were generated from the pyrolysis of 2, 2'-azobis(isobutyronitrile) under an oxygen atmosphere, and the reaction products from curcumin were followed by HPLC. The reaction at 70 degrees C gave several products, three of which were structurally identified to be vanillin, ferulic acid, and a dimer of curcumin after their isolation. The dimer was a newly identified compound bearing a dihydrofuran moiety, and its chemical structure was elucidated using spectroscopic analyses, especially 2D NMR techniques. A mechanism for the dimer production is proposed and its relation to curcumin's antioxidant activity discussed. The time course and gel permeation chromatography studies of the reaction were also investigated, and the results indicate that the dimer is a radical-terminated product in the initial stage.  相似文献   

Molecular phylogeny and diversity of Myanmar and Bhutan mithun based on mtDNA sequences     

Kazuaki TANAKA  Tatsuya TAKIZAWA  Hayato MURAKOSHI  Tashi DORJI  Maung Maung NYUNT  Yoshizane MAEDA  Yoshio YAMAMOTO  Takao NAMIKAWA 《Animal Science Journal》2011,82(1):52-56

The mithun (Bos frontalis), synonymous with mithan and gayal, is considered to be a domesticated form of gaur (B. gaurus). However, there has been a controversy concerning its origin. In an effort to address this issue, the mitochondrial cytochrome b (cytb) genes of 20 mithun from Myanmar and 13 from Bhutan were sequenced to trace its maternal origin. Seven cytb haplotypes were found in the 33 mithun, and the phylogenetic tree for these haplotypes clearly showed three embranchments involving five gaur types, a B. indicus type, and a B. taurus type. Sixteen Myanmar and 12 Bhutan mithun had gaur haplotypes, while a B. indicus haplotype was found in three Myanmar and one Bhutan mithun. The B. taurus haplotype was detected in a single Myanmar animal. These results demonstrated that the principal maternal origin of mithun was gaur and suggested that it was directly domesticated from gaur. However, some introgression of domestic cattle existed in current mithun populations. The presence of cattle mtDNA raised the question of how many cattle nuclear genes might have been integrated into the gene pool of mithun.  相似文献   

Three-dimensional culture of keratinocytes and the formation of basement membrane for canine footpad substitute   总被引：1，自引：0，他引：1  

Yamazoe K  Miyamoto S  Hikosaka Y  Kitagawa K  Watanabe K  Sakai H  Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):611-617

A pad equivalent for a dog was prepared as a substitute for the loss of footpad. In addition to the time course of formation on epidermal morphogenesis, we investigated expressions of alpha(6) integrin subunit as adhesive molecule, and laminin and type IV and VII collagens as extracellular matrices of basement membrane components. Epithelium of the pad equivalent was thick enough to be easily confirmed at 5 days at the air-liquid interface, but many creases appeared on it at 7 days, and it shrank at 10 and 14 days. Keratinocytes were increased in 4 to 5 cell layers at 1 day at the air-liquid interface, differentiating into basal cell layer. Granular and corneal cell layers were confirmed until 5 days, and maintained their shape at least until 14 days. Alpha 6 integrin was expressed at almost the same fluorescent intensity as native pad tissue at 1 day at the dermal-epidermal junction. Laminin and type IV collagen were intermittently expressed at 5 and 10 days, respectively, at the dermal-epidermal junction, and at 14 days the fluorescence showed almost the same intensity as native pad tissue. The expression of type VII collagen was discontinuous at 2 days at the dermal-epidermal junction, but remained as it was at 14 days. The present findings suggested that although the formation of anchoring fibrils in basement membrane was incomplete, the pad equivalent in the dog was reconstructed similar to a native pad by epidermal morphogenesis.  相似文献   

Oral pharmacokinetics of the acidic drugs,diclofenac and sulfamonomethoxine in male Shiba goats     

Mohamed ELBADAWY  Takara SAKIYAMA  Rania ABOHATAB  Kazuaki SASAKI  Minoru SHIMODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):21-26

In the present study, we examined the oral pharmacokinetics of the acidic drugs, diclofenac (DF) and sulfamonomethoxine (SMM), which have different physicochemical properties, in Shiba goats. DF and SMM were intravenously and orally administered to 5 male goats using a crossover design. The T_max of DF and SMM were reached 1.5 and 5.6 hr after they have been orally administered, respectively, and this was followed by their slow elimination. The elimination of both drugs was markedly faster after being intravenously rather than orally administered, which indicated flip-flop phenomena after the oral administration. The mean absorption times (MATs) of DF and SMM were 6 and 15 hr, respectively. This slow absorption may have been due to slow gastric emptying in goats. The large difference observed in MATs between DF and SMM may have been because DF, which is more lipophilic than SMM, was partly absorbed from the forestomach. Therefore, these results suggest that the absorption of highly lipophilic drugs from the forestomach may be markedly high in Shiba goats. In case of drugs whose elimination is quite fast, their efficacies may appear from the early stage after oral administration even in ruminants, because elimination rate is the determinant factor of T_max in flip-flop phenomena. Such drugs may be used orally even in ruminants.  相似文献