Meticillin-resistant Staphylococcus aureus (MRSA) is an important nosocomial and community-acquired pathogen with zoonotic potential. The relationship between MRSA in humans and companion animals is poorly understood. This study presents a quantitative exposure assessment, based on expert opinion and published data, in the form of a second order stochastic simulation model with accompanying logistic regression sensitivity analysis that aims to define the most important factors for MRSA acquisition in dogs. The simulation model was parameterised using expert opinion estimates, along with published and unpublished data. The outcome of the model was biologically plausible and found to be dominated by uncertainty over variability. The sensitivity analysis, in the form of four separate logistic regression models, found that both veterinary and non-veterinary routes of acquisition of MRSA are likely to be relevant for dogs. The effects of exposure to, and probability of, transmission of MRSA from the home environment were ranked as the most influential predictors in all sensitivity analyses, although it is unlikely that this environmental source of MRSA is independent of alternative sources of MRSA (human and/or animal). Exposure to and transmission from MRSA positive family members were also found to be influential for acquisition of MRSA in pet dogs, along with veterinary clinic attendance and, while exposure to and transmission from the veterinary clinic environment was also found to be influential, it was difficult to differentiate between the importance of independent sources of MRSA within the veterinary clinic. The implementation of logistic regression analyses directly to the input/output relationship within the simulation model presented in this paper represents the application of a variance based sensitivity analysis technique in the area of veterinary medicine and is a useful means of ranking the relative importance of input variables. 相似文献
ObjectiveTo assess the effect of halothane (H), isoflurane (I) or sevoflurane (S) on the bispectral index (BIS), and the effect of the addition of meperidine in dogs subjected to ovariohysterectomy.Study designProspective, randomized, blinded, clinical trial.AnimalsForty-eight female mixed-breed dogs, with weights varying from 10 to 25 kg.MethodsAll dogs were premedicated with acepromazine (A) (0.1 mg kg?1 IM) or A and meperidine (M) (3 mg kg?1 IM) and they were divided into six groups of eight animals (AH, AMH, AI, AMI, AS, and AMS). Fifteen minutes after premedication they were anesthetized with propofol (5 mg kg?1 IV) and then orotracheally intubated. Anesthesia was maintained with halothane, isoflurane or sevoflurane, respectively. The BIS, variables were recorded at 15 minutes after administering pre-anesthetic medication (T0); 10 minutes of anesthesia maintenance (T1); right ovarian pedicle ligation (T2); muscle suturing (T3); skin suture (T4) and 10 minutes after terminating the inhalant anesthetic (T5), respectively.ResultsBIS values were decreased at all times when compared to the baseline values in all groups (p < 0.05). In the comparative assessment between groups, the values obtained at T0 and T1 were similar for all groups. At T2, the values in AMH were lower than those obtained in AI, AMI and AS (p < 0.05). At the same time significantly higher values were found for AI when compared to AMS (p < 0.01). There was a correlation between the bispectral index and the expired anesthetic fraction in all groups.Conclusions and clinical relevanceWithin groups given the same inhalant anesthetic the bispectral index was a good indicator for the degree of hypnosis in dogs, indicating a good correlation with the amount of anesthetic and the nociceptive stimulation. BIS was a less reliable indicator of relative anesthetic depth when comparing equipotent end-tidal concentrations between the three inhalants. 相似文献
The objective of this study was to examine the relationship between easily measured, potential physiological and physical indicators of feed efficiency including metabolic hormones, metabolites, ultrasonic muscle and fat measures with performance and efficiency traits in performance tested pedigree beef bulls (n = 302; initial bodyweight 493 (SD = 64) kg). Animals were offered a high energy concentrate ad libitum plus 1.5 kg fresh weight grass hay daily and individual feed intake was measured for 70 days. Blood samples were collected by coccygeal venipuncture at the start and end of the performance test period and analysed for plasma concentrations of IGF-I, insulin, leptin and various metabolites. Similarly, ultrasonic muscle and fat depths were measured at the start and end of the test period. Residual feed intake (RFI) was computed for each animal as the residuals from a multiple regression model regressing dry matter intake (DMI) on average daily gain (ADG) and mid-test BW0.75 (MWT). Overall ADG, DMI, feed:gain (F:G) and RFI were 1.91 (SD = 0.29), 10.10 (SD = 1.31), 5.37 (SD = 0.84) and 0.00 (SD = 0.78), respectively. Residual feed intake was strongly correlated with DMI (r = 0.67) and moderately correlated with F:G (r = 0.40). Moderate positive correlations ranging from 0.3 to 0.6 were estimated between ultrasonic measures of final fat and muscle depth and their respective gains over the test period with DMI, ADG and RFI. There was no evidence of a strong association between any of the plasma analytes measured and RFI at either sampling times. However, end of test insulin was negatively correlated (r = − 0.14) with RFI. Final IGF-I concentration was negatively associated with DMI (r = − 0.14) and F:G (r = − 0.15). End of test circulating leptin concentration was positively correlated with DMI (r = 0.14) and F:G (r = 0.15). Plasma glucose concentration at the end of test was negatively related to DMI (r = − 0.21) and F:G (r = − 0.21). A positive relationship was observed between end of test plasma urea concentration and DMI (r = 0.30). Overall, the correlation coefficient estimates between the potential blood markers and measures of intake, performance and efficiency were weak and generally not different from zero. This suggests that it is unlikely that measurement of these metabolic indicators, per se, will be useful in the early identification of feed efficient animals. 相似文献
Canine B‐cell lymphoma is a clinically heterogenous disease; however, it is generally treated as a single disease entity. The purpose of this clinical trial was to prospectively evaluate naïve canine B‐cell lymphoma patients using histopathology, flow cytometry (FC) and a standardized chemotherapy protocol to better define subsets of this disease that may respond differently to treatment. Sixty‐four dogs with naïve multicentric B‐cell lymphoma were treated with a standardized 19‐week CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) chemotherapy protocol. Most of the dogs (84.3%) were diagnosed with diffuse large B‐cell lymphoma (DLBCL), followed by nodal marginal zone (7.8%), small B‐cell (4.7%), Burkitt‐like (1.6%) and follicular lymphoma (1.6%). FC confirmed the diagnosis of B‐cell lymphoma in all cases. There were no clear phenotyping differences between the subtypes of B‐cell lymphoma detectable by our FC panel. The histologic subtypes in this study exhibited a range of forward scatter values on flow cytometry, but all of the DLBCL cases were higher than a value of 469, while the only cases with a lower forward scatter value were follicular lymphoma and diffuse small B‐cell lymphoma. Dogs with DLBCL had a significantly better objective response rate to the CHOP protocol (96.3%) than the non‐DLBCL subtypes (70%, P = .024). The median progression‐free survival time for patients with DLBCL (233 days) was significantly longer than that of all other histopathologic subgroups combined (163 days, P = .0005). 相似文献
