Identification of an allele attributable to formation of cucumber-like flavor in wild tomato species (Solanum pennellii) that was inactivated during domestication     

Matsui K  Ishii M  Sasaki M  Rabinowitch HD  Ben-Oliel G 《Journal of agricultural and food chemistry》2007,55(10):4080-4086

Carbon 6 (C6)-aldehydes formed by fatty acid 13-hydroperoxide lyase (13HPL) specific to fatty acid 13-hydroperoxides (13-HPO) are important flavor constituents in fresh tomato fruits. C9-aldehydes are usually formed by 9/13HPL showing dual specificity to 9- and 13-HPOs and are scarcely found in tomato fruits. Mature red fruits of one of the introgression lines, IL1-4, generated by hybridization of a cultivated tomato (Solanum lycopersicon) to its wild relative Solanum pennellii, form high amounts of C9-aldehydes upon homogenization. The IL1-4 fruits showed high 9/13HPL activity. One of the genes isolated from IL1-4 showed a high similarity to plant 9/13HPLs. Recombinant proteins expressed in Escherichia coli showed 9/13HPL activity. Cleaved amplified polymorphic sequence analyses indicated that the gene was specific to IL1-4 and S. pennellii. S. lycopersicon had a gene having high similarity to the S. pennellii gene. It was absent in IL1-4. Among the differences of amino acid residues found between the two genes, a Cys to Ser exchange may be responsible for the inactivation of resultant protein product of S. lycopersicon gene because the Cys is an essential amino acid residue for HPL activity. From these observations, it could be assumed that a tomato gene corresponding to S. pennellii 9/13HPL gene had been inactivated through domestication of tomatoes.  相似文献   

Pathogenicity of Pepper mild mottle virus Is Controlled by the RNA Silencing Suppression Activity of Its Replication Protein but Not the Viral Accumulation     

Tsuda S  Kubota K  Kanda A  Ohki T  Meshi T 《Phytopathology》2007,97(4):412-420

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Seasonal parasitism of native egg parasitoids of brown marmorated stink bug (Halyomorpha halys) in Japan     

Kamiyama  Matthew T.  Matsuura  Kenji  Hata  Toshimitsu  Yoshimura  Tsuyoshi  Yang  Chin-Cheng Scotty 《Journal of pest science》2022,95(3):1067-1079

Journal of Pest Science - The invasive, Halyomorpha halys (Hemiptera: Pentatomidae), is a severe economic insect pest native to East Asia. A strong effort has been made to identify natural egg...  相似文献   

Compensatory Cellular Reactions to Nonsteroidal Anti-Inflammatory Drugs on Osteogenic Differentiation in Canine Bone Marrow-Derived Mesenchymal Stem Cells     

Namgil OH  Sangho KIM  Kenji HOSOYA  Masahiro OKUMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):629-636

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Prediction of bending stiffness and moment carrying capacity of sugi cross-laminated timber     

Minoru Okabe  Motoi Yasumura  Kenji Kobayashi  Kazuhiko Fujita 《Journal of Wood Science》2014,60(1):49-58

Cross-laminated timber (CLT) panels consist of several layers of lumber stacked crosswise and glued together on their faces. Prototype sugi CLT floor panels were manufactured and bending tests were carried out under the different parameters of lumber modulus of elasticity (MOE), number of layers, thickness of lumber and thickness of CLT panels. On the basis of above tests, bending stiffness and moment carrying capacity were predicted by Monte Carlo method. MOE of lumber was measured by using grading machine and tensile strength of lumber was assumed to be 60 % of bending strength based on the obtained bending test. Bending stiffness EI of CLT panels could be estimated by adopting composite theory and equivalent section area. Experimental moment carrying capacity showed 12 % higher value than the calculated moment carrying capacity by average lumber failure method, and also showed 45 % higher value than the calculated moment carrying capacity by minimum lumber failure method due to the reinforcement of the outer layer by the neighboring cross layer.  相似文献   

Growth changes in bighand thornyhead <Emphasis Type="Italic">Sebastolobus macrochir</Emphasis> off the Pacific coast of northern Honshu,Japan     

Tsutomu Hattori  Yoji Narimatsu  Masaki Ito  Yuji Ueda  Kunihiro Fujiwara  Daiji Kitagawa 《Fisheries Science》2007,73(2):341-347

ABSTRACT:     The biomass of bighand thornyhead Sebastolobus macrochir was increased by the high recruitment success of the 1999–2002 year classes off the Pacific coast of northern Honshu, Japan. In this study, the growth of bighand thornyhead was examined over a 9-year period from 1996 to 2004 in this area. The growth of the 1999 year class and the 2000–2002 year classes was reduced at 3 and 2 years old, respectively, while the 1999–2002 year classes were smaller than the 1993–1998 year classes. In 2-, 3- and 4-year-old fish, the relationship between abundance and mean standard length was expressed by negative linear regressions, while fish became smaller when abundance of the year class was larger. Mean bottom temperatures were stable at depths of 350–900 m; variations in water temperature were small in the main distribution area of bighand thornyhead. We discuss the factors affecting the growth of bighand thornyhead via changes in the demersal fish community and feeding habits.  相似文献   

Comparison of the Occurrence of Transportation-associated Fever in 2 Years Old Thoroughbreds before and after Introduction of Prophylactic Marbofloxacin Administration     

Yoshiro ENDO  Takeru TSUCHIYA  Kentaro AKIYAMA  Naoya TAKEBE  Kenji NAKAI  Kenji KOROSUE  Mutsuki ISHIMARU  Nao TSUZUKI  Seiji HOBO 《Journal of Equine Science》2014,25(4):79-81

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Increase in plasma total antioxidant capacity of grazing Japanese Black heifers and cows in forestland in Japan     

Satoshi Haga  Hiroshi Ishizaki  Miwa Nakano  Seiji Nakao  Kiyoshi Hirano  Yoshito Yamamoto  Miya Kitagawa  Hiroyuki Sasaki  Yoshihiro Kariya 《Animal Science Journal》2014,85(2):135-142

Blood total antioxidant capacity (TAC) has become a key bio‐marker for animal health. Forest‐grazing cattle are known to forage various native plants that have high TAC. This study evaluated differences of plasma TAC between forest‐grazing (FG) and pasture‐grazing cattle (PG). Experiment 1 monitored the plasma TAC levels of 32 Japanese Black cattle. The level in PG did not change throughout the grazing period. However, that in FG, which increased from summer, was significantly higher than that in PG through fall (P < 0.05). In experiment 2, we used nine Japanese Black heifers and investigated their blood antioxidant parameters and the TAC in plants that the cattle consumed in late June and September. The plasma TAC levels in FG were significantly higher than those in PG in both periods (P < 0.05). Plasma levels of lipid peroxidation in FG tended to be lower than that in PG (P = 0.098). Furthermore, the TAC levels in various species of shrubs and trees consumed by FG were higher than those in pasture grasses. Results of this study show that plasma TAC of grazing Japanese Black cattle in forestland increase from summer through fall.  相似文献   

Effect of shrimp head protein hydrolysates on the state of water and denaturation of fish myofibrils during dehydration   总被引：1，自引：0，他引：1  

Yaowalux?Ruttanapornvareesakul  Misako?Ikeda  Kenji?HaraEmail author  Kazufumi?Osako  Orawan?Kongpun  Yukinori?Nozaki 《Fisheries Science》2005,71(1):220-228

ABSTRACT:   To utilize fisheries waste products as food materials with functional properties, shrimp head protein hydrolysates (SHPH) from three species of shrimp, that is, Northern pink shrimp ( Pandalus eous ), Endeavour shrimp ( Metapenaeus endeavouri ) and Black tiger shrimp ( Penaeus monodon ), were produced by enzymatic hydrolysis using endopeptidase derived from Bacillus subtilis and exopeptidase derived from Aspergillus oryzae at a level of 0.1% (w/w). SHPH were rich in protein (90–91%) and amino acids (71–84%) but little fat (0.01–0.02%). The average molecular weight of SHPH was 300–1400. The effect of 5% SHPH (dry basis) addition on the state of water and denaturation of lizard fish myofibrils (Mf) during the dehydration process was evaluated by the desorption isotherm and the Ca-ATPase activity, and compared with the effect of sodium glutamate (Na-Glu). SHPH decreased the water activity and the Ca-ATPase inactivation, and increased monolayer sorbed water and multilayer sorbed water of Mf, although these effects of SHPH were smaller than those of Na-Glu. These findings suggest that the SHPH suppressed dehydration-induced denaturation of myofibrillar protein by stabilizing the hydrated water surrounding myofibrils.  相似文献   

Evaluation of technologies for sequestering carbon in agricultural land by using organic filter underdrains     

Iwao Kitagawa  Yasutaka Tsukamoto 《Paddy and Water Environment》2014,12(2):287-294

Technology for storing carbon in the subsoil of agricultural land using organic filter materials in underdrains was evaluated as an activity for sequestering CO₂ to mitigate global warming. First, the quantity of carbon remaining in wood chips and rice husks in underdrains was determined over 11 years after construction. Moreover, the quantity of CO₂ emissions from the construction of two underdrain types was calculated. Then, a survey was conducted to investigate the effect on greenhouse gas emissions of the filter material. The results indicate that greenhouse gas emissions would not increase. The quantity of storage carbon in the wood chip filter material of underdrains during their 15-year service life was estimated to be 6.76 tCO₂eq ha^?1. Rice husks, in contrast, were found ineffective in storing carbon. Therefore, the selection of the filter material for underdrains is important. The value of storage carbon in the wood chip filter material is similar to the amount of carbon sequestered by no-till farming, which is now being implemented as a global warming countermeasure based on soil management in agricultural land.  相似文献