Identification of food-derived collagen peptides in human blood after oral ingestion of gelatin hydrolysates     

Iwai K  Hasegawa T  Taguchi Y  Morimatsu F  Sato K  Nakamura Y  Higashi A  Kido Y  Nakabo Y  Ohtsuki K 《Journal of agricultural and food chemistry》2005,53(16):6531-6536

In the present study, we identified several food-derived collagen peptides in human blood after oral ingestion of some gelatin hydrolysates. Healthy human volunteers ingested the gelatin hydrolysates (9.4-23 g) from porcine skin, chicken feet, and cartilage after 12 h of fasting. Negligible amounts of the peptide form of hydroxyproline (Hyp) were observed in human blood before the ingestion. After the oral ingestion, the peptide form of Hyp significantly increased and reached a maximum level (20-60 nmol/mL of plasma) after 1-2 h and then decreased to half of the maximum level at 4 h after the ingestion. Major constituents of food-derived collagen peptides in human serum and plasma were identified as Pro-Hyp. In addition, small but significant amounts of Ala-Hyp, Ala-Hyp-Gly, Pro-Hyp-Gly, Leu-Hyp, Ile-Hyp, and Phe-Hyp were contained.  相似文献   

Collection,mapping, and annotation of over 28,000 cDNA clones from japonica rice   总被引：2，自引：0，他引：2  

Rice Full-Length cDNA Consortium;National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team  Kikuchi S  Satoh K  Nagata T  Kawagashira N  Doi K  Kishimoto N  Yazaki J  Ishikawa M  Yamada H  Ooka H  Hotta I  Kojima K  Namiki T  Ohneda E  Yahagi W  Suzuki K  Li CJ  Ohtsuki K  Shishiki T;Foundation of Advancement of International Science Genome Sequencing & Analysis Group  Otomo Y  Murakami K  Iida Y  Sugano S  Fujimura T  Suzuki Y  Tsunoda Y  Kurosaki T  Kodama T  Masuda H  Kobayashi M  Xie Q  Lu M 《Science (New York, N.Y.)》2003,301(5631):376-379

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Atmospheric methane and nitrous oxide of the Late Pleistocene from Antarctic ice cores     

Spahni R  Chappellaz J  Stocker TF  Loulergue L  Hausammann G  Kawamura K  Flückiger J  Schwander J  Raynaud D  Masson-Delmotte V  Jouzel J 《Science (New York, N.Y.)》2005,310(5752):1317-1321

The European Project for Ice Coring in Antarctica Dome C ice core enables us to extend existing records of atmospheric methane (CH4) and nitrous oxide (N2O) back to 650,000 years before the present. A combined record of CH4 measured along the Dome C and the Vostok ice cores demonstrates, within the resolution of our measurements, that preindustrial concentrations over Antarctica have not exceeded 773 +/- 15 ppbv (parts per billion by volume) during the past 650,000 years. Before 420,000 years ago, when interglacials were cooler, maximum CH4 concentrations were only about 600 ppbv, similar to lower Holocene values. In contrast, the N2O record shows maximum concentrations of 278 +/- 7 ppbv, slightly higher than early Holocene values.  相似文献   

Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus     

Watanabe T  Tomizawa S  Mitsuya K  Totoki Y  Yamamoto Y  Kuramochi-Miyagawa S  Iida N  Hoki Y  Murphy PJ  Toyoda A  Gotoh K  Hiura H  Arima T  Fujiyama A  Sado T  Shibata T  Nakano T  Lin H  Ichiyanagi K  Soloway PD  Sasaki H 《Science (New York, N.Y.)》2011,332(6031):848-852

Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specific sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated region (DMR) of the imprinted mouse Rasgrf1 locus, but not other paternally imprinted loci. A retrotransposon sequence within a noncoding RNA spanning the DMR was targeted by piRNAs generated from a different locus. A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1.  相似文献   

Asynchronous climate changes in the North Atlantic and Japan during the last termination     

Nakagawa T  Kitagawa H  Yasuda Y  Tarasov PE  Nishida K  Gotanda K  Sawai Y;Yangtze River Civilization Program Members 《Science (New York, N.Y.)》2003,299(5607):688-691

Pollen records from the annually laminated sediment sequence in Lake Suigetsu, Japan, suggest a sequence of climate changes during the Last Termination that resembles that of the North Atlantic region but with noticeable differences in timing. An interstadial interval commenced a few centuries earlier [approximately 15,000 years before the present (yr B.P.)] than the North Atlantic GI-1 (B?lling) event. Conversely, the onset of a Younger Dryas (YD)-like cold reversal (12,300 to 11,250 yr B.P.) postdated the North Atlantic GS-1 (YD) event by a few centuries. Climate in the Far East during the Last Termination reflected solar insolation changes as much as Atlantic influences.  相似文献   

A rubberlike stretchable active matrix using elastic conductors     

Sekitani T  Noguchi Y  Hata K  Fukushima T  Aida T  Someya T 《Science (New York, N.Y.)》2008,321(5895):1468-1472

By using an ionic liquid of 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide, we uniformly dispersed single-walled carbon nanotubes (SWNTs) as chemically stable dopants in a vinylidene fluoride-hexafluoropropylene copolymer matrix to form a composite film. We found that the SWNT content can be increased up to 20 weight percent without reducing the mechanical flexibility or softness of the copolymer. The SWNT composite film was coated with dimethyl-siloxane-based rubber, which exhibited a conductivity of 57 siemens per centimeter and a stretchability of 134%. Further, the elastic conductor was integrated with printed organic transistors to fabricate a rubberlike active matrix with an effective area of 20 by 20 square centimeters. The active matrix sheet can be uniaxially and biaxially stretched by 70% without mechanical or electrical damage. The elastic conductor allows for the construction of electronic integrated circuits, which can be mounted anywhere, including arbitrary curved surfaces and movable parts, such as the joints of a robot's arm.  相似文献   

湖羊起源及系统地位的研究   总被引：14，自引：0，他引：14  

耿荣庆  常洪  杨章平 《西北农林科技大学学报(自然科学版)》2002,30(3):21-24

应用中心产区典型群简单随机抽样法 ,检测控制血液酶和其他蛋白质变异的 14个结构基因座上31个等位基因的频率 ,并引用国内外 2 2个绵羊群体 11个座位的研究资料 ,以探讨湖羊的起源及系统地位。研究表明 ,湖羊与蒙古利亚的绵羊群体亲缘关系相对较近 ,两者可能在更早的世代具有共同的起源。这个结果与已知的历史事实吻合。  相似文献   

Curing and degradation processes of cement-bonded particleboard by supercritical CO<Subscript>2</Subscript> treatment     

Rohny Setiawan Maail  Kenji Umemura  Hideo Aizawa  Shuichi Kawai 《Journal of Wood Science》2011,57(4):302-307

This study examined the effects of supercritical CO₂ treatment on the curing and degradation of cementbonded particleboard (CBP). Significant correlations were found between the supercritical CO₂ treatment and mechanical properties during both curing and degradation processes. Internal bond (IB) strength, modulus of rupture (MOR), and modulus of elasticity (MOE) values of CBP achieved their maximums by supercritical CO₂ treatment in 30 min. These conditions indicated that supercritical CO₂ treatment accelerates the curing process rapidly and enhances the mechanical properties of the CBP. However, these values decreased in treatment from 60 min to 10 days and had a negative effect on board performance, indicating that supercritical CO₂ treatment over a longer time span leads to degradation of the CBP. Furthermore, X-ray diffractometry (XRD), thermal gravimetry (TG-DTG), and scanning electron microscopy (SEM) observation clarified that the mechanisms of degradation are directly affected by the mineralogical composition of the system, in par ticular, by the calcium carbonate content as caused by carbonation.  相似文献   

Fatty acid composition of ruminal bacteria and protozoa,and effect of defaunation on fatty acid profile in the rumen with special reference to conjugated linoleic acid in cattle     

Halima SULTANA  Kenji MIYAZAWA  Shuhei KANDA  Hisao ITABASHI 《Animal Science Journal》2011,82(3):434-440

Objectives of this study were to compare fatty acid (FA) composition of ruminal bacterial (B) and protozoal (P) cells, and to investigate effect of protozoa on FA profile in the rumen of cattle. Three cows were used to prepare ruminal B and P cells. Four faunated and three defaunated cattle (half‐siblings) were used to study effect of protozoa on ruminal FA profile. Proportions of C16:0 and C18:0 in total fatty acids in B cells were 20.7% and 37.4%, whereas those in P cells were 33.4% and 11.6%, respectively. Proportions of trans‐vaccenic acid (VA) and cis‐9, trans‐11 conjugated linoleic acid (CLA) in B cells were 3.9% and 1.0%, and those in P cells were 5.5% and 1.6%, respectively, being higher in P cells. Proportions of C18:1, C18:2 and C18:3 in P cells were two to three times higher than in B cells. Proportions of unsaturated fatty acids, VA and CLA in B cells of faunated cattle were higher than those of defaunated. VA and CLA in the ruminal fluid of faunated were also 1.6 to 2.5 times higher than those of defaunated. This tendency was similar for cell‐free fraction of ruminal fluid. These results indicate that protozoa contribute greatly in VA and CLA production in the rumen.  相似文献   

Effects of growth and dietary crude protein level until first insemination on milk production during first lactation in Holstein heifers     

Takashige ISHII  Kenji KAWASHIMA  Haruo ORIBE  Hiromi UEDA  Toshiya HASUNUMA  Kiyoshi AKIYAMA  Hirofumi NAKAYAMA  Mitsunori KURIHARA  Fuminori TERADA  Shiro KUSHIBIKI 《Animal Science Journal》2011,82(6):741-746

To decrease the age at first calving in Holsteins, the effects of average daily body weight gain (ADG) and crude protein (CP) level until first insemination on growth performance and milk production were examined. The MM group had a target ADG of 0.75 kg and received a diet with a CP level of 14%. The HM and HH groups had a target ADG of 1 kg; both these groups received a diet with CP levels 14% and 16%, respectively. The ADG in the HM and HH groups was 1.1 kg, whereas in the MM group it was 0.97 kg (P < 0.01). The HM and HH groups showed no differences in withers height at body weight 350 kg. The ages at first calving in MM, HM and HH groups were 23.1, 21.0 and 21.8 months, respectively. The HM and HH groups had lower milk yield at day 305 than the MM group (P < 0.01). These results suggest that growth performance until first insemination should be maintained at an ADG of 0.97 kg or less with a CP level of approximately 14%, to shorten time until first insemination and prevent the decrease of milk yield.  相似文献