Downregulation of male-specific cytochrome P450 by profenofos     

Moustafa GG  Ibrahim ZS  Ahmed MM  Ghoneim MH  Sakamoto KQ  Ishizuka M  Fujita S 《The Japanese journal of veterinary research》2008,56(2):109-118

The health hazards of individual organophosphorus insecticides have been characterized by their acute toxicity, mainly by investigating their cholinesterase inhibition. However, the chronic effects of most of these toxicants on the drug-metabolizing enzymes have not been investigated. Profenofos (O-4-bromo-2-chlorophenyl O-ethyl S-propyl phosphorothioate) is an organophosphorus pesticide widely used in cotton cultivation. In the present study, we investigated the effect of profenofos on male-specific cytochrome P450 (CYP) enzymes in adult Wistar rats. We orally administered 17.8 mg/kg body weight, twice weekly for 65 days. Profenofos downregulated levels of hepatic and testicular CYP2C11 and CYP3A2 mRNA and protein expression. Testicular aromatase (CYP19A) mRNA was decreased in the profenofos-treated rats compared to controls. Overall, the present study suggests that profenofos acts as an endocrine disruptor of male-specific CYP enzymes and affects testosterone concentration, which implicates its deleterious effects on animal or human males chronically exposed to organophosphorus pesticide.  相似文献   

Molecular variability of the genomes of capilloviruses from apple, Japanese pear, European pear, and citrus trees     

Magome H  Yoshikawa N  Takahashi T  Ito T  Miyakawa T 《Phytopathology》1997,87(4):389-396

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Variation in Virulence of Plasmodiophora brassicae in Japan Tested with Clubroot-resistant Cultivars of Chinese Cabbage (Brassica rapa L. ssp. pekinensis)     

Yasuhisa Kuginuki  Hiroaki Yoshikawa  Masashi Hirai 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(4):327-332

The differential hosts of Williams (1966) and the European Clubroot Differential (ECD) (Buczacki et al., 1975) have been used commonly to identify populations of Plasmodiophora brassicae, which causes clubroot disease in Brassica crops. However, some of these hosts showed intermediate and fluctuating scores to most populations from Japan. Therefore, these hosts could not be used to provide a clear classification in Japan. We have tried to clarify the genetic diversity in pathogenicity of P. brassicae in Japan using Japanese clubroot-resistant (CR) F₁ hybrid (F₁) cultivars and lines of Brassica rapa. The responses of some CR F₁ cultivars were very clear. Four groups of field populations in Japan were recognized using the CR F₁ cultivars. The clear response obtained here may depend largely on the genetic purity of the F₁ cultivars. Moreover, it is possible to classify some of these Japanese populations into the same race using the Williams set and ECD 01 to ECD 05. The present differential hosts may be useful in the study of European populations of P. brassicae. The response of the differential hosts suggests that there are several major CR genes in B. rapa. It is suggested that pyramiding CR genes would be useful in breeding CR cultivars that can overcome the breakdown of the present CR cultivars of Chinese cabbage.  相似文献   

A transdisciplinary research of coastal fisheries co-management: the case of the hairtail <Emphasis Type="Italic">Trichiurus japonicus</Emphasis> trolling line fishery around the Bungo Channel,Japan     

Mitsutaku Makino  Shingo Watari  Taro Hirose  Kentaro Oda  Masahito Hirota  Atsushi Takei  Michio Ogawa  Hiroshi Horikawa 《Fisheries Science》2017,83(6):853-864

Under the coastal fisheries co-management regime in Japan, local fishers play important roles in deciding on and implementing local management measures. Therefore, using the case study of a hairtail trolling line fishery, we conducted a transdisciplinary research with fishers, processors, government officers, etc. Taking social and biological factors into account, we defined management criteria and targets (resource, economic, human community, and local food culture) in collaboration with local fishers. We chartered local fishing vessels to determine parameters that were easily understood by local fishers. We established a local consultative committee comprising fishers, processors, and distributors, and developed a fish distribution strategy to nurture the local hairtail food culture. The integrated effects of various management scenarios were evaluated by the operating model. We found that co-ordinated management by both the trolling line fishery and purse seiners in the neighbouring prefecture was required to achieve targets. Two realistic scenarios are recommended: a conservative scenario that could achieve targets even with few strong year classes, and an adaptive scenario that protects strong year classes, but relies on a higher frequency of these. We shared these results with the local fishers and government officers, which led to actual improvements in management measures.  相似文献   

Enhancement and Biological Characteristics Related to Aerobic Biodegradation of Toluene with Co-Existence of Benzene     

Miho Yoshikawa  Ming Zhang  Koki Toyota 《Water, air, and soil pollution》2016,227(9):340

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Quantifying morphological and genetic variation of sympatric populations to guide conservation of endangered,micro‐endemic springsnails          下载免费PDF全文

Cayla R. Morningstar  Kentaro Inoue  Makiri Sei  Brian K. Lang  David J. Berg 《水产资源保护:海洋与淡水生态系统》2014,24(4):536-545

Work-related increases in titer of Campylobacter jejuni antibody among workers at a chicken processing plant in Miyazaki prefecture,Japan, independent of individual ingestion of edible raw chicken meat     

Elisabet TANGKONDA  Meiko KUBO  Satoshi SEKIGUCHI  Taisuke SHINKI  Satomi SASAKI  Kentaro YAMADA  Takako TANIGUCHI  Torrung VETCHAPITAK  Naoaki MISAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1306

Workers in poultry abattoirs may be frequently exposed to Campylobacter jejuni, which is a leading cause of bacterial food poisoning in Japan. The present study was conducted to measure the titers of IgG and IgA antibodies against C. jejuni among 104 female workers in a chicken processing plant in Miyazaki prefecture, Japan. Information regarding habitual ingestion of raw chicken meat and potential occupational risk factors was collected using a questionnaire. Acid extracts of four C. jejuni strains representing the genotypes most dominant in Miyazaki were used as antigens. The levels of both immunoglobulins measured by ELISA were not correlated with ingestion of edible raw chicken meat, the amount consumed in one sitting, or its frequency. Although age was correlated with antibody levels, the length of employment was not. Furthermore, the IgG and IgA levels in workers at the evisceration step were significantly higher than those at other locations in the plant. To identify the bacterial proteins recognized by the workers’ IgG and IgA antibodies, Western blotting followed by LC/MS was conducted. Flagellin was identified as the common protein recognized in the sera of workers for whom ELISA demonstrated both the highest and lowest antibody levels. We concluded that the titers of IgG and IgA against C. jejuni in workers at the processing plant had been increased by occupational exposure to Campylobacter, regardless of raw chicken meat ingestion.  相似文献   

Effect of fatty acids added to the milk replacer on white scour and excretion of fatty acids in Holstein calves     

Keiji OKADA  Yoshie GOTO  Takehiro FURUKAWA  Kentaro IKUTA  Jun YASUDA 《Animal Science Journal》2009,80(5):542-545

In order to examine the relationship between white scour and fatty acids, we added fatty acids to the milk replacer. Twenty healthy Holstein calves were divided into 4 groups, five calves per group; a control group with no fortified fatty acid, and 3 groups fed either with oleic, stearic, or palmitic acid, respectively. The calves were fed milk replacer (5% of the calf's body weight) twice a day but the fatty acids (2 wt % of milk replacer) were added only once. The fecal and blood samples were obtained at 0, 12, 24, 36, and 48 h after feeding of the acids. All five calves in the palmitic acid group, and 3 out of 5 calves each in the stealic and the oleic acid groups had whitish feces after feeding fatty acid. The stearic acid group had a significantly elevated stearic acid concentration in the feces during 24–36 h compared to the pre-feeding level. The fecal concentration of palmitic acid increased significantly at 24–36 h in the palmitic acid group. We concluded that the long-chain saturated fatty acids are one of the causes of white scour in calves.  相似文献   

External beam radiation therapy for canine intracranial meningioma          下载免费PDF全文

Hiroto Yoshikawa  Monique N. Mayer 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(1):97-100

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Pathological and phylogenetic features of prevalent canine distemper viruses in wild masked palm civets in Japan     

Takayama I  Kubo M  Takenaka A  Fujita K  Sugiyama T  Arai T  Yoneda M  Sato H  Yanai T  Kai C 《Comparative immunology, microbiology and infectious diseases》2009,32(6):539-549

Ten wild masked palm civets infected with canine distemper virus (CDV), captured in Japan from 2005 to 2007, were histopathologically and phylogenetically analyzed. Phylogenetic analysis based on the amino acid sequences of the H protein of two CDV isolates from masked palm civets revealed that the two isolates were classified into the clade of recent isolates in Japan. Histopathologically marked lesions of virus encephalitis were present in the brain, whereas gastrointestinal lesions were absent or at a mild degree. The distribution of the lesions resembles that of recent CDV cases in dogs. Therefore, recent CDV infections in masked palm civets could be caused by recently prevalent CDV in dogs. The possibility of the masked palm civet as a spreader of CDV among wildlife is also discussed.  相似文献