Presence of less heavily encapsulated Klebsiella pneumoniae capsular type 1 in semen of healthy stallions and cervical swabs of mares suffering from metritis and comparison of virulence between heavily and less heavily encapsulated strains     

N Kikuchi  N Takayanagi  Y Kosaka  T Hiramune  R Yanagawa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(2):313-323

  相似文献   

Integrative diagnosis of the needle nematode Longidorus jonesi affecting forest in southern Japan     

J. E. Palomares‐Rius  T. Kikuchi  C. Cantalapiedra‐Navarrete  P. Castillo 《Forest Pathology》2014,44(3):246-249

  相似文献   

Interleukin-1 receptor antagonist expression in the equine endometrium during the peri-implantation period     

S. Haneda  K. Nagaoka  Y. Nambo  M. Kikuchi  Y. Nakano  M. Matsui  Y. Miyake  J.N. Macleod  K. Imakawa   《Domestic animal endocrinology》2009,36(4):209-218

To identify factors involved in the establishment of pregnancy in the mare, endometrium was collected from day 13 (day 0 = day of ovulation) cyclic and day 13, 19, and 25 pregnant animals. From initial cDNA subtraction studies, interleukin-1 receptor antagonist (IL-1RN) mRNA was found as a candidate molecule expressed uniquely in the pregnant endometrium. Expression of IL-1RN mRNA was markedly increased in day 19 and 25 gravid endometrium. In situ hybridization analysis revealed that IL-1RN mRNA was localized to the glandular epithelium. Interleukin-1 receptor antagonist (IL-1RN) protein was found in the extracts of day 25 gravid endometrium and was immunochemically localized to the glandular epithelium/luminal cavity of the pregnant uterus. High concentrations of estradiol-17β (E₂) were detected in day 25 conceptuses. Concentrations of E₂ were higher in the gravid endometrial portion than in other endometrial regions. On the other hand, progesterone concentrations did not differ among endometrial samples analyzed. Furthermore, the expression of IL-1RN mRNA was up-regulated in endometrium culture samples treated with 10 ng/mL E₂ and 10 ng/mL progesterone. In the analysis of related gene expression, increased amounts of IL-1α and IL-6 mRNA were also found in the day 25 gravid endometrium; however, these expressions in endometrial culture samples were not up-regulated by the steroid treatment. These results indicate that expression of IL-1RN in the endometrium is likely regulated by E₂ and progesterone and suggest that IL-1RN regulates the degree of IL-1 signal transduction and thereby plays an important role in the establishment of equine pregnancy.  相似文献   

Plasma Atrial Natriuretic Peptide in Healthy Calves and Calves with Congenital Heart Disease     

Y. Hori  M. Kikuchi  A. Nakagawa  T. Yonezawa  H. Miura  Y. Ohnami 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(3):653-656

Background: The basic and clinical implications of evaluating plasma atrial natriuretic peptide (ANP) concentration in calves are unknown.
Objective: To investigate the relationship between the plasma ANP concentration and left ventricular end-diastolic pressure (LVEDP) in healthy calves subjected to volume overload (Study 1), and to compare the plasma ANP concentration in calves with or without heart disease (Study 2).
Animals: Six healthy calves were used in Study 1; disease calves and sick calves with (n = 9) and without congenital heart disease (CHD) (n = 9) were used in Study 2.
Methods: In Study 1, LVEDP in anesthetized calves was manipulated by IV administration of acetated Ringer's solution (rate of 100 mL/kg/h for 20 minutes) and furosemide. In Study 2, disease calves were identified by blood examination and echocardiography or pathological examination. The plasma ANP concentration was determined by a chemiluminescence enzyme immunoassay for human α-ANP.
Results: In Study 1, preloading significantly increased the plasma ANP concentration (36 ± 20–185 ± 156, P < .01) and LVEDP (−11 ± 7–2 ± 12, P < .01) from the baseline. Furthermore, plasma ANP concentrations were strongly correlated with LVEDP ( r = 0.61). In Study 2, the plasma ANP concentration was significantly higher in the calves with CHD than in the calves without heart disease (220 [67–970] versus 31 [10–86]; mean [range], P < .001).
Conclusions and Clinical Importance: Measurement of plasma ANP concentrations in calves can provide additional information useful for predicting hemodynamic abnormalities.  相似文献   

Changes in circulating and testicular levels of inhibin A and B during postnatal development in bulls     

Kaneko H  Matsuzaki M  Noguchi J  Kikuchi K  Ohnuma K  Ozawa M 《The Journal of reproduction and development》2006,52(6):741-749

We investigated testicular and circulating levels of dimeric inhibins in Holstein bulls from the infantile to postpubertal periods (5 to 50 weeks of age) and examined the relationship between the profiles of circulating dimeric inhibins and FSH. Concentrations of total inhibin and inhibin B in the testis were highest at 4 to 5 weeks of age but decreased gradually as the bulls aged. Testicular inhibin A levels showed a gradual decline to a nadir at 15 to 26 weeks of age, but by 39 weeks, they were high again. The contents of total inhibin, inhibin A, and inhibin B per testis generally increased with age. Fractionation of testicular homogenates obtained from 15-week-old bulls by a combination of immunoaffinity chromatography and SDS-PAGE confirmed the presence of two major molecular weight forms (32 and 45 kDa) of dimeric inhibins in the testes. Circulating levels of total inhibin and inhibin A showed a significant increase in bulls at around 10 to 14 weeks of age compared to the levels between 5 and 7 weeks of age but decreased thereafter. However, immunoreactivity for inhibin B was not detected in the peripheral circulation, probably because of low sensitivity of the inhibin B assays. The concentrations of plasma FSH were high at 5 weeks of age but declined to lower levels between 11 and 40 weeks, and then increased from 41 weeks onward. There was no significant correlation between the plasma levels of FSH and inhibin A or total inhibin. The results clearly indicate that the bull testis produces inhibin A and B and secretes at least inhibin A into the circulation during postnatal development. However, the profile of circulating FSH in bulls shows no reciprocal relationship with the inhibin A or total inhibin profile during the postnatal period.  相似文献   

Successful long term culture of immature porcine sertoli cells in the reconstructed testicular cell cord     

Noguchi J  Ohnuma K  Ozawa M  Karja NW  Fahrudin M  Somfai T  Kikuchi K  Kaneko H 《The Journal of reproduction and development》2006,52(3):383-389

The ultimate goal of this study was to establish an in vitro system to produce sperms. To pursue this goal, immature porcine testicular cells were cultured in stereostructural form and cultured testicular cord was investigated morphologically. At 4 weeks of age, the seminiferous tubules of the porcine testes consisted of undifferentiated germ cells (gonocytes and undifferentiated spermatogonia) and immature Sertoli cells. The interstitial tissue was largely occupied by Leydig cells. The testes were enzymatically digested, and the dispersed cells were encapsulated with alginate either immediately or after freeze-thawing. The resulting testicular cell cords were cultured for up to 10 weeks. After 2 weeks of culture, Sertoli cells, which were identified by their inhibin-positive reaction in immunohistochemistry, and Leydig cells, which were identified by their morphological characteristics, were observed in the cords. Neither undifferentiated nor differentiated types of germ cells were detected. The number of cells in the cords progressively decreased during the culture period. In order to discover the fate of the Sertoli cells, the level of inhibin in the spent media was determined. Inhibin in the media was at a detectable level after 2 days of culture. The levels increased and peaked at 2 weeks. When frozen-thawed testicular cells were applied to the culture, the peak level was maintained for over 8 weeks, in contrast to the gradual decrease of inhibin level when fresh cells were cultured. These results indicate that the culture conditions can sustain the survival of Sertoli cells. Further improvement is required for proliferation and differentiation of germ cells.  相似文献   

Pilot-scale test of a soil filter for treatment of malodorous gas     

R. Kikuchi 《Soil Use and Management》2000,16(3):211-214

Abstract. A pilot-scale test confirmed that malodorous air containing 600 mg l⁻¹ ammonia can be passed through a soil filter with 98.5% removal efficiency. Furthermore, the concentrations of methyl mercaptan, dimethyl sulfide, dimethyl disulfide, actaldehyde, and trimethyl amine were also significantly reduced using soil as a filter. The tests provide technical information for planning of industrial-scale soil filters: linear velocity, bed depth, properties of soil, reliability, and effects of soil deodorization on various types of odorous substances. It is concluded that the soil can be an effective filter to remove noxious and malodorous gases derived from industrial processes.  相似文献   

Heavy-ion-induced mutants in sweet pepper isolated by M1 plant selection     

Ichiro Honda  Kaori Kikuchi  Satoshi Matsuo  Machiko Fukuda  Hiroyuki Saito  Hiromichi Ryuto  Nobuhisa Fukunishi  Tomoko Abe 《Euphytica》2006,152(1):61-66

We examined the effects of heavy-ion bombardment on mutagenesis in sweet pepper (Capsicum annuum L.). Dose–response studies indicated that 10 Gy irradiation of ¹²C or ²⁰Ne ions on dry seeds is suitable for inducing mutations in plants. From ²⁰Ne-irradiated M₁ plants, putative mutants included two dwarf plants and one plant whose pericarp was yellow were isolated. Phenotypes of their M₂ progeny were similar to those of the M₁ plants and did not segregate. F₁ plants resulting from reciprocal crosses between the mutants and wild-type plants showed the wild-type phenotype, but phenotypes of F₂ and BC₁F₁ segregated at 1:3 (mutant:wild) and 1:1, respectively. These crossing experiments indicate that the three mutants have monogenic recessive mutations in nuclear genes. In light of these data, we discuss the effectiveness of using heavy-ion bombardment to mutate sweet peppers.  相似文献   

Simultaneous liquid chromatographic determination of residual synthetic antibacterials in cultured fish     

N Nose  Y Hoshino  Y Kikuchi  M Horie  K Saitoh  T Kawachi  H Nakazawa 《Journal of the Association of Official Analytical Chemists》1987,70(4):714-717

A liquid chromatographic method is described to determine simultaneously the following 11 synthetic antibacterial agents used in a fishery: nitrofuran derivatives furazolidone, nifurpirinol, difurazone, and furamizole; sulfa drugs sulfamerazine, sulfisozole, sulfamonomethoxine, and sulfadimethoxine; and, oxolinic, nalidixic, and piromidic acids. A Nucleosil C18 column was used with tetrahydrofuran-acetonitrile-phosphoric acid-water (29 + 1 + 0.06 + 69.94) as the mobile phase. Pretreatment of the fish meat sample with acetone extraction and alumina column cleanup gave good separation of the LC peaks without interference from any other components. Recovery of the antibacterial agents was ca 80%. The lower limit of detection of the drugs was 1-2 ng for 10 microL injection.  相似文献   

Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis     

Nagata T  Yamada H  Du Z  Todoriki S  Kikuchi S 《Journal of agricultural and food chemistry》2005,53(4):1022-1030

  相似文献