Development and comparison of serologic methods for diagnosing chicken anemia virus infection.   总被引：1，自引：0，他引：1  

C M Lamichhane  D B Snyder  T Girschick  M A Goodwin  S L Miller 《Avian diseases》1992,36(3):725-729

This paper describes the development of an indirect immunoperoxidase assay (IIP) and an indirect enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to chicken anemia virus (VAC). The IIP assay developed used CAV-infected MDCC-MSB1 cells for detecting antibody to CAV, whereas the ELISA utilized gradient-purified immunoadsorbed CAV as the target antigen. The IIP and ELISA were compared with the standard indirect immunofluorescent antibody (IFA) assay, which is more conventionally used to screen chicken serum for antibodies against CAV. Comparative test results of 185 field samples of chicken serum by these three methods were in agreement 84% of the time. Both IFA and IIP assays yielded fewer positive tests than did the ELISA. IFA and IIP assays were in agreement 93% of the time, as compared with 91% agreement of IIP and ELISA results, or 84% agreement for comparative IFA and ELISA results.  相似文献   

Micropropagation of western hemlock (Tsuga heterophylla [Raf.] Sarg.) from embryonic explants     

Indra S. Harry  Chin-Yi Lu  Kiran K. Sharma  Trevor A. Thorpe 《New Forests》1994,8(1):1-13

An in vitro propagation protocol is described for western hemlock, an important forestry species in Canada. For shoot bud induction, embryonic explants were placed initially on one-third strength Schenk and Hildebrandt medium containing 5 M N⁶-benzyladenine (BA) or 5 M BA in combination with either 5 M kinetin or 5 M 2-isopentenyl adenine for 14 days. The explants were transferred to basal medium without cytokinins for 3 weeks, and then to basal medium containing 0.05% activated charcoal. Elongating shoots were subcultured every 4 weeks on charcoal medium. Shoots, 10 mm in stem height, were rooted either in agar or sterilized peat/perlite (1:1). Up to 70% of the shoots formed roots when they were transferred to the latter, moistened with 1/2 strength Gresshoff and Doy medium containing 5 M -naphthaleneacetic acid. About 90% of the plantlets survived transfer to greenhouse conditions.  相似文献   

Canine brucellosis in a dog imported into the UK     

Dunne J  Sehgal K  McMillan A  Perret L 《The Veterinary record》2002,151(8):247

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Genetic dissection of root architectural traits by QTL and genome‐wide association mapping in rapeseed (Brassica napus)     

Aysha Kiran  Abdul Wakeel  Rod Snowdon  Wolfgang Friedt 《Plant Breeding》2019,138(2):184-192

Variation in root architecture is essential for the adaptation of plants to target environments. A non‐destructive gel‐based mini‐rhizotron system was used for root architecture trait phenotyping. This system has facilitated the visualization of root architectural traits in large genotype collection of rapeseed including 94 double haploid (DH) lines from “Express617” x “V8” and 439 inbred lines (ASSYST diversity set). A high‐density Express617‐V8 linkage map was used for quantitative trait loci (QTLs) identification in DH population based on standard composite interval mapping. 6K SNPs were analysed for association mapping of root traits in ASSYST diversity set. A large variation, broad segregation and medium–low heritability of root architectural traits, primary root length and growth rate, lateral root number, lateral root length and lateral root density, were observed. In the double haploid population, 11 QTL regions, and in the diversity set, 38 significant marker–trait associations were detected. Significant marker–trait associations proved that these are quantitatively inherited traits controlled by multiple genes which revealed to proceed for genetic improvement and selection of rapeseed lines with improved root system.  相似文献   

Differential Expression Pattern Of MADS Box Genes in Floral Whorls of Garcinia indica     

Patil  Reshma V.  Pawar  Kiran D. 《Journal of Crop Science and Biotechnology》2019,22(4):363-369

Journal of Crop Science and Biotechnology - Flower formation is one of the main developmental stages in the life cycle of flowering plants that can be used as a model system to elucidate the...  相似文献   

The use of piperonyl butoxide and MGK-264 to improve the efficacy of some plant-derived molluscicides     

Kiran Singh  Ajay Singh  D. K. Singh 《Pest management science》1998,54(2):145-149

Synergism of an oil of Azadirachta indica, a powdered extract of Allium sativum bulbs and an oleoresin of Zingiber officinale rhizomes by piperonyl butoxide and MGK-264 was studied against the snails Lymnaea acuminata and Indoplanorbis exustus. The active components of these plant-derived molluscicides, respectively azadirachtin, allicin and [6]gingerol, were also combined with these synergists. Both piperonyl butoxide and MGK-264 enhanced the toxicity of all of the test compounds. The response of snails to the synergised mixtures was both time- and dose-dependent. © 1998 Society of Chemical Industry  相似文献   

Morphological and molecular analyses of Rosa damascena × R. bourboniana interspecific hybrids     

Kiran Kaul  S. KarthigeyanD. Dhyani  Navtej KaurR.K. Sharma  P.S. Ahuja 《Scientia Horticulturae》2009

Rosa damascena Mill is the most important scented rose species cultivated for rose oil production. Rosa bourboniana L. (Edward rose), a related species, is popular on account of its longer blooming period and ease of propagation. With an aim to combine the oil quality of R. damascena and recurrent flowering habit of R. bourboniana, two cultivars (Jwala and Himroz) of R. damascena were crossed with R. bourboniana. The F1 hybrids obtained were evaluated using morphological, random amplified polymorphic DNA (RAPD) and microsatellite (SSR) markers. Twenty-two selected RAPD and three SSR primer pairs were utilized for hybrid identification. According to presence or absence of bands RAPD and SSR markers were classified into seven types of markers. The bands specific for the pollen parent and occurring in the hybrids were good markers to confirm the hybridity. The non-parental bands expressing uniquely in hybrids were effective in distinguishing the hybrids from each other. Cluster analysis, based on Jaccard's similarity coefficient using unweighted pair group method based on arithmetic mean (UPGMA), reliably discriminated the hybrids into two main clusters. These results indicate the practical usefulness of RAPD and SSR markers in hybrid identification in scented roses. The approach is advantageous for its rapidity and simplicity, for identification of hybrids at the juvenile stage. One of the studied morphological traits – prickle density, can also complement in the identification of interspecific hybrids between R. damscena (♀) and R. bourboniana (♂).  相似文献   

Overcoming self-incompatibility in Ipomoea cairica by IAA     

Sood  Renuka  Prabha  Kiran  Govil  Suman  Gupta  Shrish C. 《Euphytica》1982,31(2):333-339

Summary Ipomoea cairica Sweet (Convolvulaceae) exhibits sporophytic self-incompatibility. This has partially been overcome under in vitro conditions, by treating the pollen and/or stigma with 10^-5–10^-1M indole-3-acetic acid (IAA), the optimum being 10^-2M. The self-pollen. which otherwise does not even stick to stigma. germinates after self-pollination provided only one or both the partners are treated. The pollen tubes not only penetrate stigmatic papillae but also traverse the whole length of the style, at least in optimum experimental combinations.  相似文献   

International provenance trial of Neem (<Emphasis Type="Italic">Azadirachta indica</Emphasis>) in the terai region of Nepal     

D. Lamichhane  H. B. Thapa 《Agroforestry Systems》2011,81(1):37-43

An international provenance trial of Neem (Azadirachta indica A. Juss.) was established in July 1996 at Butwal Research Station of western Terai region of Nepal. It comprised the seed sources of 23 provenances collected from 10 countries including Nepal. A randomized complete block design was used for the experiment with two replications in an area of 1.5 hectares. The objectives of this trail were similar to that of other provenance trials under the International Neem Network. The assessments were made for survival rate, diameter at breast height (DBH), height, crown diameter and straightness of stem at age 5 and age 10. Average survival rates of the provenances in the two assessments were 69.74 and 63.5% respectively. The survival rate ranged from 33% for Kuliyapitiya, Sri Lanka to 92% for Ramannagudu, India. The four provenances Ramannagudu, Sagar, Sunyani, and Lamahi showed ≥80% survival rate. Similarly, Sunyani, Ban Bo, Yezin, Ramannagudu and Doitao showed best performance for DBH. The mean DBH of provenances was 8.74 cm (SD = 2.07). The five provenances namely, Sunyani, Yezin, Ban Bo, Ghaati and Bandia were the best performer for height. The mean height and SD of provenances were 6.29 m and 0.79 respectively. Sunyani had the widest crown diameter at both ages. Ban Nong showed the highest score for the straightness of main stem. Out of the two local provenances of Nepal, Lamahi showed better performance than Geta in many characteristics.  相似文献   

DNA barcoding of important fruit tree species of agronomic interest in the genus Garcinia L. from the Western Ghats     

Anerao  Jayesh  Jha  Vikas  Shaikh  Nishaat  Shivalkar  Apurva  Nityanand  Aishwarya  Sawant  Dishanand  Rao  G. Ramachandra  Mangaonkar  Kiran  Deodhar  Manjushri  Desai  Nitin 《Genetic Resources and Crop Evolution》2021,68(8):3161-3177

Genetic Resources and Crop Evolution - Garcinia L. is a pantropically distributed genus with high species richness in South East Asia. It is a tropical evergreen plant with distinct morphological...  相似文献