Synthesis and bioactivity of some new N-aryl/alkyl/cyclohexyl-N'-(2,3-dihydro-2-oxo-4H-benz[e][1,3,2]oxazaphosphorin-2-yl) ureas     

Kiran YB  Gunasekar D  Devendranath Reddy C  Suresh Reddy C  Tran K  Le T  Berlin KD  Srinivasan S  Charitha Devi M 《Pest management science》2005,61(10):1016-1023

Several new substituted oxazaphosphorinyl urea derivatives of the type RR'P(O)NHC(O)NHR' were synthesized from alpha-(3-chloro-4-fluoroanilino)-o-cresol by reaction with chlorides of aryl/alkyl/cyclohexyl carbamidophosphoric acids in the presence of triethylamine at 0-50 degrees C. Their significant insecticidal and antimicrobial activity and promotion of Rhizobium bacteria growth in the soil without effect on the host tissue suggests their possible commercial application as ecofriendly pesticides and antimicrobial agents.  相似文献   

Heifers express G‐protein coupled receptor 153 in anterior pituitary gonadotrophs in stage‐dependent manner          下载免费PDF全文

Kiran Pandey  Onalenna Kereilwe  Hiroya Kadokawa 《Animal Science Journal》2018,89(1):60-71

We recently found that orphan G‐protein‐coupled receptor (GPR)153 is expressed in the anterior pituitary (AP) of heifers, leading us to speculate that GPR153 colocalizes with gonadotropin‐releasing hormone receptor (GnRHR) in the plasma membrane of gonadotrophs and is expressed at specific times of the reproductive cycle. To test this hypothesis, we examined the coexpression of GnRHR, GPR153, and either luteinizing hormone or follicle‐stimulating hormone in AP tissue and cultured AP cells by immunofluorescence microscopy. GPR153 was detected in the gonadotrophs, and was colocalized with GnRHR in the plasma membrane. GPR153 was also detected in the cytoplasm of cultured gonadotrophs. Real‐time PCR and western blot analyses found that expression was lower (P < 0.05) in AP tissues during early luteal phase as compared to pre‐ovulation or late luteal phases. The 5′‐flanking region of the GPR153 gene contained a consensus response element sequence for estrogen, but not for progesterone. These data suggest that some, but not all GPR153 colocalizes with GnRHR in the plasma membrane of gonadotrophs, and its expression changes stage‐dependently in the bovine AP.  相似文献   

Performance evaluation of five detection tests for avian influenza antigen with various avian samples     

Chua TH  Ellis TM  Wong CW  Guan Y  Ge SX  Peng G  Lamichhane C  Maliadis C  Tan SW  Selleck P  Parkinson J 《Avian diseases》2007,51(1):96-105

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Impact of organic manure and chemical fertilizers on artemisinin content and yield in Artemisia annua L     

Prabhakar JhaMauji Ram  M.A. KhanUsha Kiran  Mahmooduzzafar  M.Z. Abdin 《Industrial Crops and Products》2011,33(2):296-301

Artemisinin isolated from the aerial parts of Artemisia annua L. is a promising and potent antimalarial drug. It posses remarkable activity against both chloroquinine resistant as well as chloroquinine sensitive strains of Plasmodium falciparum. It is also useful in the treatment of cerebral malaria. The relatively low content of artemisinin in A. annua and unavailability of cost effective and viable synthetic protocol however, are major obstacles to the commercial production of the drug. The enhanced production of artemisinin is hence, highly desirable, which can be achieved by adequate and judicious supply of plant nutrients. The present experiment was therefore, designed to study the effect of organic manure (15 tonnes ha⁻¹) and chemical fertilizers (N₄₀₊₄₀, P₄₀, K₄₀, S₁₅₊₁₅ kg ha⁻¹; nitrogen, phosphorus, potassium and sulphur) on the accumulation of artemisinin and biomass in various plant parts through the developmental stages of A. annua L. Artemisinin yield (kg ha⁻¹) was also determined through the developmental stages of A. annua L. Artemisinin content and artemisinin yield of dried leaves were increased significantly at pre-flowering stage in the plants treated with NPKS (27.3% and 53.6%) and NPK (18.2% and 33.5%), respectively, when compared with control. Maximum dry yield of leaf ranging from 2596 to 3141 kg ha⁻¹ was observed at pre-flowering stage with various treatments.  相似文献   

Plant pathogenic bacteria in open irrigation systems: what risk for crop health?          下载免费PDF全文

J. R. Lamichhane  C. Bartoli 《Plant pathology》2015,64(4):757-766

The current global area equipped for irrigation provides 30–40% of the world's gross food output. Water used for plant irrigation may harbour plant pathogens and foster their spread. This represents a serious risk for crop health, with heavy socio‐economic consequences. More specifically to plant pathogenic bacteria (PPB), a range of species have been reported from irrigation systems and their potential role in epidemic development has long been recognized. However, only a few studies have been performed to date on the ecology of PPB in the context of water habitats. Consequently, current knowledge of the biology, ecology and epidemiology of PPB in irrigation water is poor. In light of this, an attempt is made to describe the most relevant information concerning the role of open irrigation systems in the survival and dissemination of PPB throughout the range of cultivations and the possible consequences for crop health. The information described in this paper will help to improve understanding on the overlooked role of irrigation water as a reservoir of PPB.  相似文献   

Insights into epidemiology and control of diseases of annual plants caused by the Pseudomonas syringae species complex     

Jay Ram Lamichhane  Antoine Messéan  Cindy E. Morris 《Journal of General Plant Pathology》2015,81(5):331-350

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Occurrence and characterization of a new red-pigmented variant of Curtobacterium flaccumfaciens,the causal agent of bacterial wilt of edible dry beans in Iran     

Ebrahim Osdaghi  S. Mohsen Taghavi  Habiballah Hamzehzarghani  Amal Fazliarab  Robert M. Harveson  Jay Ram Lamichhane 《European journal of plant pathology / European Foundation for Plant Pathology》2016,146(1):129-145

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SCAR markers: A potential tool for authentication of herbal drugs     

Usha Kiran  Salim Khan  Khanda Jabeen Mirza  Mauji Ram  M.Z. Abdin   《Fitoterapia》2010,81(8):969-976

Randomly Amplified Polymorphic DNA (RAPD) is easy to develop and simple molecular marker, but lack of reproducibility makes it less reliable for authentication of herbal drugs. Besides RAPD, other popular PCR and non-PCR based markers like AFLP, ISSR, SSR and RFLP are also used for authentication. However, these also have disadvantages like use of radioactive isotopes, high cost and absolute requirement of sequence information. Therefore, it is a better option to improve the reproducibility of RAPD by converting RAPD amplicons into Sequence Characterized Amplified Region (SCAR) markers. SCAR markers are easy, reliable and reproducible thus, have an advantage over RAPD markers for authentication of medicinal herbs used in the preparation of traditional medicines. These markers however, have been developed for only a few medicinal herbs. This review is an attempt to evaluate critically the role of SCAR markers in authentication of medicinal herbs used in traditional formulations.  相似文献   

Epiphytic Curtobacterium flaccumfaciens strains isolated from symptomless solanaceous vegetables are pathogenic on leguminous but not on solanaceous plants          下载免费PDF全文

E. Osdaghi  S. M. Taghavi  H. Hamzehzarghani  A. Fazliarab  R. M. Harveson  S. Tegli  J. R. Lamichhane 《Plant pathology》2018,67(2):388-398

In Iran, during 2013–16, 16 Gram‐positive corynebacteria‐like strains were recovered from the epiphytic parts of solanaceous vegetables including eggplant, pepper and tomato. The strains were recovered accidentally as a result of monitoring for other bacterial pathogens in solanaceous fields. The strains were phenotypically different from Clavibacter michiganensis strains. Although none of the strains were pathogenic on their host of isolation or on any other solanaceous plants, 12 out of 16 strains were pathogenic on common bean, cowpea, mung bean and soybean. Colonization by strains was observed on maize, zucchini, faba bean, honeydew melon, rapeseed, sugar beet and sunflower plants under greenhouse conditions. In PCR tests, the primer pair CffFOR2/CffREV4, specific for Curtobacterium flaccumfaciens pv. flaccumfaciens, enabled the amplification of the appropriately sized fragment in 12 out of 16 strains, and all 12 strains were pathogenic on dry beans. Phylogenetic analysis, using the gyrB and recA genes, showed all 16 bacterial strains clustered within several pathovars of C. flaccumfaciens. A nonpathogenic yellow‐pigmented strain (Xeu15) was clustered with the type strains of C. flaccumfaciens pv. betae and C. flaccumfaciens pv. oortii. Bacteriocin profiling assays revealed no significant differences among the pathogenic and nonpathogenic strains. Host range and population dynamics of four representative strains on 17 plant species showed population build‐up of the strains only on common bean, cowpea, wheat and red nightshade plants. The results provide important insights into the possible role of nonhost plants as reservoirs of plant pathogenic bacteria, which has important implications in plant disease epidemiology and management.  相似文献   

Epiphytic growth of Xanthomonas arboricola and Xanthomonas citri on non‐host plants          下载免费PDF全文

S. Zarei  S. M. Taghavi  H. Hamzehzarghani  E. Osdaghi  J.R. Lamichhane 《Plant pathology》2018,67(3):660-670

The population dynamics of Xanthomonas arboricola pv. pruni (Xap) and X. citri subsp. citri (Xcc) was assessed on over three dozen plant species/genotypes under field and greenhouse conditions. Both Xap and Xcc multiplied on red nightshade, black nightshade, bindweed, Chenopodium, common bean and wheat up to 20 days post‐inoculation (dpi) under greenhouse conditions. A high bacterial growth rate was observed on all (alfalfa, bindweed, Chenopodium, field mustard, millet and prickly lettuce) but one (liquorice) plant species tested under field conditions. Xap successfully proliferated on both lemon and sweet lemon up to 140 dpi, attaining a population density even higher than that of Xcc. The latter showed an increased growth rate on GxN, GF677, Ghisella 6 and Mariana 2624 rootstocks up to 140 dpi. While Xap and Xcc did not grow on pomegranate and common fig, they had a steady population growth on apple and pear plants up to 140 dpi, although the final population sizes were smaller than those observed on lemon and sweet lemon plants. The results suggest that a large number of non‐host plant species could support epiphytic populations of Xap or Xcc, which may have implications for plant disease epidemiology.  相似文献