外源人绒毛膜促性腺激素诱导黑边石斑鱼排卵的研究     

徐文刚  唐永政  张建柏  征矢野清  邹华锋 《广东农业科学》2020,47(4):122-128

【目的】了解外源人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)诱导黑边石斑鱼(Epinephelus fasciatus)排卵的影响。【方法】以成熟的黑边石斑鱼为研究对象,测量其体长、体质量和肥满度后,在2018年7—8月,使用HCG激素(剂量为200 IU/kg或500 IU/kg)注射分别对6尾雌鱼进行成熟诱导。【结果】在200 IU/kg HCG处理中,注射24 h前有5尾雌鱼的卵母细胞处于第三次卵黄球前期阶段;注射48 h后2尾雌鱼出现核移动卵母细胞,1尾雌鱼的卵母细胞为第三次卵黄球后期阶段;注射60 h后1尾雌鱼出现排卵现象,其余5尾雌鱼均为第三次卵黄球后期阶段。在200 IU/kg HCG处理的排卵个体中,注射时其卵母细胞直径为477.0 μm,注射后60 h卵母细胞的直径增加至624.4 μm。此外,经催产、排卵、受精和孵化后共获得总卵数16 906粒,受精率为68.7%,孵化率为43.0%。而500 IU/kg HCG处理的黑边石斑鱼排卵失败。【结论】使用剂量为200 IU/kg的外源HCG激素可诱导黑边石斑鱼成熟且排卵,但为了提高排卵率、受精率和孵化率,其注射剂量和效应时间还需要进一步调节。  相似文献   

DNA polymorphism in the chicken growth hormone gene: Association with egg production   总被引：3，自引：0，他引：3  

Norio KANSAKU  Asako NAKADA  Hisato OKABAYASHI  Daniel GUÉMENÉ  Urs KUHNLEIN  David ZADWORNY  Kiyoshi SHIMADA 《Animal Science Journal》2003,74(3):243-244

Restriction fragment length polymorphic markers in the chicken growth hormone (GH) gene were tested for their association with egg production traits. Alleles of GH were determined in 3 non‐inbred strains from different genetic bases. The frequency of PS1 (+) allele in the three strains was very low in contrast to the layer strain previously reported. Results of the present study indicate that the frequency of PS (+) is related to egg production and that the PS1 (+) allele may be used as a genetic marker for selection in egg production.  相似文献   

Molecular cloning of two types of spiggin cDNA in the three-spined stickleback, Gasterosteus aculeatus     

Fumie Kawasaki  Ioanna Katsiadaki  Alexander P. Scott  Takahiro Matsubara  Kiyoshi Osatomi  Kiyoshi Soyano  Akihiko Hara  Koji Arizono  Masaki Nagae 《Fish physiology and biochemistry》2003,28(1-4):425-425

Two types of spiggin cDNAs were isolated from the three-spined stickleback. Northern blot analysis revealed that glue protein is composed of multiple spiggin molecules, and that their synthesis in kidney is strongly up-regulated by androgens.  相似文献   

Cytotoxic constituents in the holdfast of cultivated Laminaria japonica     

Makoto  NISHIZAWA  Nobuaki  TAKAHASHI  Kumiko  SHIMOZAWA  Takako  AOYAMA  Kouichi  JINBOW  Yukari  NOGUCHI  Kiyoshi  HORITA  Hideo  BANDO  Takashi  YAMAGISHI 《Fisheries Science》2003,69(3):639-643

ABSTRACT:     Four steroidal ketones were isolated from the holdfast of cultivated Laminaria japonica . The structures were characterized as ergosta-4,24(28)-diene-3-one (1), ergosta-4,24(28)-diene-3,6-dione (2), stigmasta-4,24(28)-diene-3-one (3) and stigmasta-4,24(28)-diene-3,6-dione (4), by spectral data. Compounds 2 and 4 were shown to be cytotoxic against the MCF-7 human breast cancer cell, and the growth of MCF-7 was inhibited by 96% and 79%, respectively, at 10 µg/mL. It is the first report on the isolation of cytotoxic steroidal ketones from the kelp in the genus of Laminaria.  相似文献   

Response changes with growth in the gustatory receptors of young yellowtails (Seriola quinqueradiata)     

Jun Kohbara  Kiyoshi Furukawa  Hajime Taishaku  Iwao Hidaka 《Fisheries Science》2000,66(6):1106-1114

SUMMARY: Laboratory-hatched juvenile yellowtails (mean fork length, 9.3 cm) were reared for 2 months in two groups on different kinds of food, mysid and squid in one group and fish in the other, and the gustatory neural responses to seven chemicals (adenosine-5'-monophosphate, alanine, arginine, betaine, proline, tryptophan, and valine) were recorded in fish before and after the 2-month rearing period. Large variances in the responses to some chemicals were noticed in the juveniles before the rearing experiment. Mann–Whitney U -tests on the neural responses indicated significant changes in the magnitude of the responses to valine and some other chemicals relative to that for proline or tryptophan between the pre- and the two post-rearing groups. No significant differences in the response magnitude for the seven chemicals were observed for the two post-rearing groups suggesting that the response changes during the 2-month rearing might have been intrinsic and not due to specific food items in the diet. The dose–response relationship for some chemicals was also examined in the juveniles before and after the 2-month rearing. A slight lowering of threshold was noticed for alanine and valine after the rearing. Data on the responses of wild yellowtails were in support of the changing responsiveness of gustatory receptors during development of juvenile yellowtails.  相似文献   

Correction to: Precocious maturation in male tiger pufferfish Takifugu rubripes: genetics and endocrinology     

Yoshikawa  Sota  Chuda  Hisashi  Hamasaki  Masaomi  Kadomura  Kazushi  Yamada  Toshiyuki  Kikuchi  Kiyoshi  Hosoya  Sho 《Fisheries Science》2021,87(3):431-431

Fisheries Science - A correction to this paper has been published: https://doi.org/10.1007/s12562-021-01505-w  相似文献   

The original papers appeared in the Journal of Japanese Forestry Society vol. 86, no. 3 in Japanese with English abstracts     

Kenichi Kano  Kiyoshi Nishikura  Osamu Arisaka 《Journal of Forest Research》2005,10(1):79-82

Abstracts from the Journal of Japanese Forestry Society

The original papers appeared in the Journal of Japanese Forestry Society vol. 86, no. 3 in Japanese with English abstracts  相似文献   

alpha-Glucosidase inhibitory profile of catechins and theaflavins   总被引：1，自引：0，他引：1  

Matsui T  Tanaka T  Tamura S  Toshima A  Tamaya K  Miyata Y  Tanaka K  Matsumoto K 《Journal of agricultural and food chemistry》2007,55(1):99-105

To clarify the postprandial glucose suppression effect of flavonoids, the inhibitory effects of catechins and theaflavins against alpha-glucosidase (AGH) were examined in this study. It was initially demonstrated that theaflavins and catechins preferentially inhibited maltase rather than sucrase in an immobilized AGH inhibitory assay system. For the maltase inhibitory effects of theaflavins, the effects were observed in descending order of potency of theaflavin (TF)-3-O-gallate (Gal) > TF-3,3'-di-O-Gal > TF-3'-O-Gal > TF. This suggests that the AGH inhibition induced by theaflavins is closely associated with the presence of a free hydroxyl group at the 3'-position of TF as well as the esterification of TF with a mono-Gal group. In addition, the R-configuration at the 3'-position of TF-3-O-Gal showed a higher inhibitory activity than the S-configuration. As a result of a single oral administration of maltose (2 g/kg) in rats, a significant reduction in blood glucose level was observed at a dose of 10 mg/kg of TF-3-O-Gal, demonstrating for the first time that TF-3-O-Gal can suppress glucose production from maltose through inhibition of AGH in the gut.  相似文献   

Pericardial mesothelioma in a neonatal calf     

Takasu M  Shirota K  Uchida N  Iguchi T  Nishii N  Ohba Y  Maeda S  Miyazawa K  Murase T  Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):519-521

We encountered an extremely rare tumor, a pericardial mesothelioma, in a neonatal calf. The patient calf showed severe abdominal distention, and died immediately after birth. The thoracic cavity was contained a huge heart with a large amount of pericardial fluid. A number of granular and cobblestone-like nodules were dispersed over the epicardium and pericardium. The nodules consisted of papillary proliferations of neoplastic cells, and the neoplasm occasionally showed mesenchymal proliferations. Immunohistochemistry revealed that they had the characteristics of mesothelial cells (cytokeratin-and vimentin-positive), and the neoplasm was diagnosed as mesothelioma.  相似文献   

Phyto- and endogenous estrogens differently activate intracellular calcium ion mobilization in bovine endometrial cells     

Woclawek-Potocka I  Borkowski K  Korzekwa A  Okuda K  Skarzynski DJ 《The Journal of reproduction and development》2006,52(6):731-740

The main purpose of this study was to check whether phyto- and endogenous estrogens influence calcium ion mobilization [Ca(2+)](i) in bovine endometrial cells and whether this action is connected with biological effects i.e. prostaglandin (PG)F(2alpha) production. In our study we used two calcium measurement methods by comparing the microscopic method with widely used quantitative - spectrofluorometric method of [Ca(2+)](i) measurement. We also wanted to confirm whether visualization of calcium ion [Ca(2+)](i) in cells using microscopic method supported by micro image analysis (Micro Image Olympus system) reflects real, qualitative changes in the ion concentration. In both methods a cell-permeable form of fluorescent [Ca(2+)](i) indicator Fura-2 was used. Cultured bovine endometrial epithelial and stromal cells influenced by phorbol-2-myristate-13-acetate (PMA; positive control), estradiol 17-beta (E(2); endogenous estrogen) and active metabolites of phytoestrogens (environmental estrogens) were used as a model to study PGF(2alpha) secretion and [Ca(2+)](i) mobilization in the cells. Equol and para-ethyl-phenol in doses of 10(-8)-10(-6) M increased PGF(2alpha) concentration both in epithelial and stromal cells (P<0.05). In both methods, equol and para-ethyl-phenol did not cause intracellular [Ca(2+)](i) mobilization in epithelial and stromal cells (P>0.05). Both methods revealed that only E(2) and PMA induced intracellular [Ca(2+)](i) mobilization in epithelial and stromal cells (P<0.05). The results of both methods were highly correlated (P<0.001; r=0.82 for epithelial cells and r=0.89 for stromal cells). In conclusion, both methods gave approximately the same results and showed that phytoestrogens, in contrast to PMA and E(2), did not cause intracellular [Ca(2+)](i) mobilization in endometrial cells. The obtained results proved that the [Ca(2+)](i) visualization method supported by micro image analysis can produce similar results to the spectrofluorometric method.  相似文献