Mycotic bronchopneumonia in cattle     

V T Kotov  D D Novak  A M Borisov 《Veterinariia》1969,46(2):55-57

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Double Strand Breaks and Cell-Cycle Arrest Induced by the Cyanobacterial Toxin Cylindrospermopsin in HepG2 Cells     

Alja ?traser  Metka Filipi?  Matja? Novak  Bojana ?egura 《Marine drugs》2013,11(8):3077-3090

The newly emerging cyanobacterial cytotoxin cylindrospermopsin (CYN) is increasingly found in surface freshwaters, worldwide. It poses a potential threat to humans after chronic exposure as it was shown to be genotoxic in a range of test systems and is potentially carcinogenic. However, the mechanisms of CYN toxicity and genotoxicity are not well understood. In the present study CYN induced formation of DNA double strand breaks (DSBs), after prolonged exposure (72 h), in human hepatoma cells, HepG2. CYN (0.1–0.5 µg/mL, 24–96 h) induced morphological changes and reduced cell viability in a dose and time dependent manner. No significant increase in lactate dehydrogenase (LDH) leakage could be observed after CYN exposure, indicating that the reduction in cell number was due to decreased cell proliferation and not due to cytotoxicity. This was confirmed by imunocytochemical analysis of the cell-proliferation marker Ki67. Analysis of the cell-cycle using flow-cytometry showed that CYN has an impact on the cell cycle, indicating G0/G1 arrest after 24 h and S-phase arrest after longer exposure (72 and 96 h). Our results provide new evidence that CYN is a direct acting genotoxin, causing DSBs, and these facts need to be considered in the human health risk assessment.  相似文献   

Effect of supplementation with an electrolyte containing a Bacillus-based direct-fed microbial on immune development in dairy calves     

Novak KN  Davis E  Wehnes CA  Shields DR  Coalson JA  Smith AH  Rehberger TG 《Research in veterinary science》2012,92(3):427-434

Immune characteristics in 65 calves were evaluated in response to a Bacillus-based direct-fed microbial (DFM) provided in electrolyte scour treatment. Blood samples were analyzed for cell surface markers and α(1)-acid glycoprotein (AGP) concentration. AGP increased in scouring calves given electrolyte containing Bacillus at day 7 post-placement compared to scouring calves administered electrolyte alone and non-scouring calves, enhancing the inflammatory response for pathogen clearance. The Bacillus promotes T cell subsets including greater proportions of activated, mature cells (CD8(-)CD25(+), CD8(-)CD45RO(+), CD8(-)TCR1(+)) in calves given electrolyte containing Bacillus than scouring calves administered electrolyte alone and non-scouring calves. Also, the Bacillus may be alleviating inflammation at day 3 post-placement as the proportion of monocytes and granulocytes lacking L-selectin (CD172a(+)CD62L(-)) was greater in scouring calves given electrolyte compared to the other groups. Electrolyte containing Bacillus administered at the onset of scours influences components of innate and adaptive immune development during and following the scouring event.  相似文献   

The Banfield Education Program     

Lewis HB  Novak W 《Journal of veterinary medical education》2003,30(1):33-40

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Comparison of prophylactic or therapeutic dietary administration of capsaicin for reduction of Salmonella in broiler chickens     

Orndorff BW  Novak CL  Pierson FW  Caldwell DJ  McElroy AP 《Avian diseases》2005,49(4):527-533

In three experiments the effects of prophylactic or therapeutic dietary inclusion of capsaicin, the pungent component of peppers, were evaluated as a nonantibiotic alternative for reduction of Salmonella in broiler chickens through culture and morphologic assessment of cecal tissue. Expt. 1 evaluated the effects of 0 or 10 ppm purified capsaicin (CAP) in the starter phase (days 1-16) on chicks challenged with Salmonella Enteritidis (SE) on day of age. Therapeutic inclusion of 10 ppm purified CAP increased (P < 0.05) liver/spleen (L/S) and ceca positive results for SE. In Expt. 2, capsaicin oleoresin (CO) was included in the finisher diet (days 30-37) at 0, 5, or 20 ppm with SE challenge on day 31. Inclusion of 5 ppm CO increased ceca positive results for SE, and a linear decrease in cecal lamina propria thickness of SE-challenged birds was observed with increased CO concentration in the diet. Expt. 3 evaluated prophylactic CO treatment at 0, 5, or 20 ppm in starter, grower, and finisher diets for resistance to SE or Salmonella Typhimurium (ST) challenge on day 14 or 29. With challenge on day 14, 5 and 20 ppm prophylactic CO feeding reduced ceca SE positive results by 37% and 26%, respectively, and ST culture rate was reduced similarly with 5 ppm CO. Lamina propria thickness of the ceca increased with 5 ppm CO feeding in SE-challenged birds, whereas a decrease was observed in nonchallenged birds fed 5 ppm CO. Challenge on day 29 of birds fed 20 ppm CO resulted in reduced L/S positive results for SE. Lamina propria thickness decreased with 5 ppm CO and SE or ST challenge compared with nonchallenged birds fed 5 ppm. An increase was observed in ST- or SE-challenged birds fed 20 ppm CO compared with nonchallenged birds fed 20 ppm CO. No differences were observed in mast cell number in either Expt. 2 or 3. These data provide evidence that prophylactic or therapeutic dietary capsaisin differentially affects broiler susceptibility to Salmonella.  相似文献   

The power of practice: harnessing patient outcomes for clinical decision making.     

Karen Faunt  Elizabeth Lund  Will Novak 《Veterinary Clinics of North America: Small Animal Practice》2007,37(3):521-532

The practice of evidence-based medicine (EBM) relies on the ability of veterinarians to evaluate clinical outcomes. Evaluation of clinical outcomes optimizes the patient care process by transforming what is learned about a population of patients and applying it to an individual patient. Veterinarians' ability to summarize and record relevant information from each pet encounter enables outcomes analysis, thereby transforming clinical data into medical knowledge. This article describes the multiple integrated processes required to evaluate outcomes and practice EBM. As a result of the aggregation and analysis of patient outcomes, knowledge is derived that has the potential to enhance clinical decision making and client communication.  相似文献   

Properties of partially purified goat kidney beta-D-mannosidase     

R D Pearce  J W Callahan  A Novak  P B Little  J T Clarke 《The British veterinary journal》1990,146(3):270-280

Lysosomal beta-D-mannosidase (EC 3.2.1.25) was purified 6900-fold from normal goat kidney by serial Concanavalin A-Sepharose 4B and Red A dye ligand affinity chromatography, followed by anion exchange and molecular sieve high performance liquid chromatography. The relative molecular mass of the enzyme was estimated by molecular sieving to be 79,000 +/- 3000. The apparent Km for the synthetic substrate, 4-methylumbelliferyl-beta-D-mannopyranoside, was 2.3-2.8 mM and the sharp, unimodal pH optimum was 5.5. Enzyme activity was inhibited by Hg2+, Zn2+, Ag+, Co2+ and the thiol reactive agent N-ethylmaleimide. The mannose derivatives p-nitrophenyl-beta-D- thiomannopyranoside and p-aminophenyl-beta-D-thiomannopyranoside inhibited enzyme activity and may be of use as immobilized ligands in future attempts to purify beta-D-mannosidase by specific affinity chromatography.  相似文献   

Soil CO₂ flux from a norfolk loamy sand after 25 years of conventional and conservation tillage     

Philip J. Bauer  James R. Frederick  Jeff M. Novak  Patrick G. Hunt 《Soil & Tillage Research》2006,90(1-2):205-211

Tillage affects the ability of coarse-textured soils of the southeastern USA to sequester C. Our objectives were to compare tillage methods for soil CO₂ flux, and determine if chemical or physical properties after 25 years of conventional or conservation tillage correlated with flux rates. Data were collected for several weeks during June and July in 2003, October and November in 2003, and April to July in 2004 from a tillage study established in 1978 on a Norfolk loamy sand (fine-loamy, kaolinitic, thermic Typic Kandiudults). Conventional tillage consisted of disking to a depth of approximately 15 cm followed by smoothing with an S-tined harrow equipped with rolling baskets. Conservation tillage consisted of direct seeding into surface residues. Flux rates in conservation tillage averaged 0.84 g CO₂ m⁻² h⁻¹ in Summer 2003, 0.36 g CO₂ m⁻² h⁻¹ in Fall 2003, 0.46 g CO₂ m⁻² h⁻¹ in Spring 2004, and 0.86 g CO₂ m⁻² h⁻¹ in Summer 2004. Flux rates from conventional tillage were greater for most measurement times. Conversely, water content of the surface soil layer (6.5 cm) was almost always higher with conservation tillage. Soil CO₂ flux was highly correlated with soil water content only in conventional tillage. In conservation tillage, no significant correlations occurred between soil CO₂ flux and soil N, C, C:N ratio, pH, bulk density, sand fraction, or clay fraction of the surface 7.5 cm. In conventional tillage, sand fraction was positively correlated, while bulk density and clay fraction were negatively correlated with soil CO₂ flux rate, but only when the soil was moist. Long-term conservation tillage management resulted in more uniform within- and across-season soil CO₂ flux rates that were less affected by precipitation events.  相似文献   

Effects of biochars and hydrochars produced from lignocellulosic and animal manure on fertility of a Mollisol and Entisol     

J. M. Novak  K. A. Spokas  K. B. Cantrell  K. S. Ro  D. W. Watts  B. Glaz  W. J. Busscher  P. G. Hunt 《Soil Use and Management》2014,30(2):175-181

Biochar and hydrochars (HC) are emerging soil fertility amendments; however, their ability to improve fertility levels in soils possessing vastly different pedogenic characteristics has not been well investigated. In this study, several plant and manure biochars and two blended HC applied at 3.84 g/kg (ca.10 t/ha) were incubated in pots containing a highly fertile‐Mollisol (Waukegan series; Sandy‐skeletal, mixed, superactive, mesic Typic Hapludoll) and an infertile Entisol (Margate series; Siliceous, hyperthermic, Mollic Psammaquent). During the 124–125 day laboratory incubations, pots were leached four times with deionized H₂O with the leachates analysed for the concentrations of dissolved phosphorus (DP) and potassium (DK). After the incubations, both soils were analysed for fertility characteristics (i.e. pH, cation‐exchange capacity (CEC), and extractable P and K). In both soils after biochar additions, there were mixed pH and CEC responses. Both the Mollisol and Entisol treated with swine solid biochar had greater plant extractable P and K contents, which was reflective of the elevated P and K contents in the swine solid biochar. However, most biochars and HC additions to the Mollisol and Entisol had minimal impact on soil fertility characteristics indicating a low direct fertilization potential. These nutrient contents could be altered through feedstock blending to target a particular fertilizer requirement.  相似文献   

Fumonisins affect the intestinal microbial homeostasis in broiler chickens,predisposing to necrotic enteritis     

Gunther Antonissen  Siska Croubels  Frank Pasmans  Richard Ducatelle  Venessa Eeckhaut  Mathias Devreese  Marc Verlinden  Freddy Haesebrouck  Mia Eeckhout  Sarah De Saeger  Birgit Antlinger  Barbara Novak  An Martel  Filip Van Immerseel 《Veterinary research》2015,46(1)

Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0234-8) contains supplementary material, which is available to authorized users.  相似文献