Effect of cooled and chlorinated chiller water on Campylobacter and coliform counts on broiler carcasses during chilling at a middle-size poultry processing plant     

Kameyama M  Chuma T  Nishimoto T  Oniki H  Yanagitani Y  Kanetou R  Gotou K  Shahada F  Iwata H  Okamoto K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):129-133

To evaluate the effect of cooled and chlorinated chill water for Campylobacter and coliforms at a middle-size processing plant which was considered to be difficult for eliminate pathogenic bacteria on carcasses, following three conditions were examined; keeping temperature at < 20, < 10 and < 10°C, and chlorine concentration at < 50, < 50 and 50 to 70 ppm during processing in experiment 1, 2 and 3 respectively. Fifteen prechill and 15 postchill carcasses were examined in each experiment. In lower temperature of experiment 2, decreasing rate (%) of coliforms was significantly higher (P<0.01) than that in experiment 1. In higher chlorination of experiment 3, no Campylobacter was detected from all postchill carcasses.  相似文献   

Detection of tumorigenic Agrobacterium strains from infected apple saplings by colony PCR with improved PCR primers     

Kouichi Suzaki  Kouji Yoshida  Hiroyuki Sawada 《Journal of General Plant Pathology》2004,70(6):342-347

With the colony polymerase chain reaction (PCR), the specificity of newly prepared primer sets VCF2/VCR2, VCF3/VCR3, VCF4/VCR4, and VCF5/VCR5 to Ti or Ri plasmids in Agrobacterium strains were compared to that of the conventional set VCF/VCR. At first, control strains, which consisted of a nonpathogenic strain and phytopathogenic strains carrying Ti or Ri plasmid, were used. VCF3/VCR3 and VCF5/VCR5 were highly specific to all the phytopathogenic strains, whereas the others were not. These two primer sets were superior to VCF/VCR in their specificity with colony PCR to tumorigenic Agrobacterium strains isolated from apple saplings.  相似文献   

Characterization of tetraploid plants regenerated via protoplast culture of Iris fulva and their crossability with Japanese irises     

Kouichi Inoue  Tomomi Kato  Asami Nobukuni  Hisato Kunitake  Tsutomu Yabuya   《Scientia Horticulturae》2006,110(4):334-339

Characteristics such as flower form, size and color of outer and inner perianths, anthocyanins in outer perianths, size, color and fertility of pollen and self-fertility of diploid and tetraploid lines regenerated via protoplast culture of Iris fulva were examined and compared with those of the diploid wild line. Among these characteristics, flower form, inner and outer perianth sizes of the tetraploid lines were noticeable, because these lines had upward flower forms and bigger flowers than diploid lines. Furthermore, reciprocal crosses between diploid or tetraploid lines of I. fulva and I. ensata and those of I. fulva and I. laevigata were performed. Three seedlings were obtained from the cross of tetraploid I. fulva × diploid I. laevigata through embryo rescue. One of them was identified as the interspecific hybrid between tetraploid I. fulva and I. laevigata by flow cytometoric (FCM), cytological and molecular (RAPD) analyses. This is the first report on production of hybrids from these lines. I. fulva has unique brown flowers, and this trait could be very useful for flower color breeding of I. laevigata which lacks this color. Therefore, the hybrid of I. fulva (4×) × I. laevigata may be the best available gene source for brown color breeding of this species.  相似文献   

Incidence of viruses in <Emphasis Type="Italic">Alstroemeria</Emphasis> plants cultivated in Japan and characterization of <Emphasis Type="Italic">Broad bean wilt virus-2, Cucumber mosaic virus</Emphasis> and <Emphasis Type="Italic">Youcai mosaic virus</Emphasis>     

Shin-ichi Fuji  Nanae Mochizuki  Masashi Fujinaga  Makoto Ikeda  Kouichi Shinoda  Seiji Uematsu  Hiromitsu Furuya  Hideki Naito  Fumiyoshi Fukumoto 《Journal of General Plant Pathology》2007,73(3):216-221

Alstroemeria plants were surveyed for viruses in Japan from 2002 to 2004. Seventy-two Alstroemeria plants were collected from Aichi, Nagano, and Hokkaido prefectures and 54.2% were infected with some species of virus. The predominant virus was Alstroemeria mosaic virus, followed by Tomato spotted wilt virus, Youcai mosaic virus (YoMV), Cucumber mosaic virus (CMV), Alstroemeria virus X and Broad bean wilt virus-2 (BBWV-2). On the basis of nucleotide sequence of the coat protein genes, all four CMV isolates belong to subgroup IA. CMV isolates induced mosaic and/or necrosis on Alstroemeria. YoMV and BBWV-2 were newly identified by traits such as host range, particle morphology, and nucleotide sequence as viruses infecting Alstroemeria. A BBWV-2 isolate also induced mosaic symptoms on Alstroemeria seedlings.  相似文献   

The four-line model of the Triangular Running Skyline system: Theory and numerical examples     

Yasushi Suzuki  Tadashi Kawai  Kouichi Kanzaki 《Journal of Forest Research》1996,1(3):117-122

The generalized static solution of the Triangular Running Skyline (TRS) system has been derived. A pair of tail spars with a haulback line enables the carriage of the TRS system to move around over the triangular area formed by connecting the bases of the three spars with each other,i.e., the two tail spars and one head spar. From the head spar, the main and slack-pulling lines stretch toward the carriage to move up and down the load. The theory derived in this paper separately evaluates the line lengths and line tensions of the main and slack-pulling lines (the four-line model), while the previous theory treats these two lines as a single equivalent line (the three-line model). Four numerical examples provided in this paper ascertained the accuracy of the two procedures of the four-line model. A part of this paper was orally presented at the Second Annual Meeting of the Japanese Forest Engineering Association, November 18, 1995, Tokyo. This English title is a tentative translation form the original Japanese by the authors of this paper.  相似文献   

Stimulatory effect of plasma samples from fattening cattle on adipogenesis‐related gene expression in preadipocyte cells          下载免费PDF全文

Noriaki Shoji  Ryo Saito  Tatsuya Nakano  Kazuki Saito  Keisuke Sumiyoshi  Michael T. Rose  Natumi Okada  Kouichi Watanabe  Hisashi Aso 《Animal Science Journal》2015,86(7):698-706

It is desirable to produce beef with high levels of monounsaturated fatty acids (MUFA), as this is related to fat softness and palatability. However, the physiology of MUFA synthesis in bovine fat during the fattening process remains to be established. In this study, in order to elucidate the relationship between plasma components and the fatty acid composition of intramuscular fat, we investigated the effect of plasma obtained from fattening cattle on the messenger RNA (mRNA) expressions of the adipogenesis‐related gene in a clonal bovine intramuscular preadipocyte line (BIP cells). The mRNA expressions of stearoyl‐CoA desaturase, adipocyte Protein 2, peroxisome proliferator‐activated receptor gamma and sterol regulatory element‐binding protein 1 in BIP cells were significantly higher following treatment with those plasma samples collected from the cattle with the highest diaphragmatic unsaturated fatty acids to saturated fatty acids ratio (US/S). Furthermore, the concentration of nonesterified fatty acid (NEFA) in the plasma samples had an inverse correlation with carcass diaphragmatic US/S. These results indicate that cattle with a low ratio of US/S in fat may be discriminated from the population of fattening cattle before slaughter by measuring the effect of their plasma on gene expression in BIP cells as well as their plasma concentration and composition of NEFA.  相似文献   

Measurement of Carbonic Anhydrase Isozyme VI (CA-VI) in Bovine Sera,Saliva, Milk and Tissues     

Nishita T  Tanaka Y  Wada Y  Murakami M  Kasuya T  Ichihara N  Matsui K  Asari M 《Veterinary research communications》2007,31(1):83-92

Concentrations of bovine carbonic anhydrase isozyme VI (CA-IV) in bovine serum, saliva, normal milk, colostrum, submandibular gland, liver, and mammary gland were determined. CA-VI was purified from bovine saliva and an antibody to CA-VI was generated. The concentrations of CA-VI in the saliva (7.8 ± 7.9 μg/ml), serum (2.1± 5.7 ng/ml), milk (7.9 ± 12.1 ng/ml), submandibular gland (284.7 μg/g protein), liver (921.0 ± 180.7 ng/g protein) and mammary gland (399.6 ± 191.2 ng/g protein) were determined by ELISA. No seasonal change in CA-VI levels was observed in normal milk. The concentration of CA-VI in colostrum (day 1 post partum) was 119 ng/ml and decreased rapidly by 1 month following birth. Mammary gland contained much smaller amounts than the submandibular gland. CA-VI mRNA was detected in the liver and mammary gland of cow by RT-PCR. The ELISA used in this study proved to be a precise and sensitive method for determining CA-VI concentrations in saliva, serum, milk and tissue specimens from cows. The ELISA may enable the study of changes in CA-VI associated with hereditary or metabolic disorders of the salivary gland, mammary gland and liver using small samples of saliva, serum or milk.  相似文献   

Immunohistolocalization and gene expression of the secretory carbonic anhydrase isozymes (CA-VI) in canine oral mucosa, salivary glands and oesophagus     

Kasuya T  Shibata S  Kaseda M  Ichihara N  Nishita T  Murakami M  Asari M 《Anatomia, histologia, embryologia》2007,36(1):53-57

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Mode of bleaching phytotoxicity of herbicidal diphenylpyrrolidinones     

Hitoshi Ogawa  Isao Yamada  Kiyoshi Arai  Kangetsu Hirase  Kouichi Moriyasu  Christian Schneider  Gerhard Sandmann  Peter Bger  Ko Wakabayashi 《Pest management science》2001,57(1):33-40

A mode of action study of herbicidal diphenylpyrrolidinones was carried out through carotenoid analyses in intact Scenedesmus cells and by a cell‐free plant‐type phytoene desaturase assay using Escherichia coli transformants. A series of forty‐eight diphenylpyrrolidinones decreased the carotenoid content of Scenedesmus cells in the light and inhibited phytoene desaturase. The relationship between substituents at various positions and inhibition of phytoene desaturase is discussed. Using very active bleaching diphenylpyrrolidinones, a 10⁻⁵ M concentration affected neither the ζ‐carotene desaturase nor the protoporphyrinogen‐IX oxidase. Although some differences in their inhibitory activity were found between the in vivo and cell‐free assays, it is concluded that the compounds are essentially bleachers affecting carotenoid biosynthesis in plants. Enzyme kinetics studies with recombinant phytoene desaturase revealed a non‐competitive inhibition with respect to the substrate phytoene. A competition against the inhibitor was shown by the cofactor NADP⁺, suggesting an interaction of pyrrolidinones at the cofactor‐binding site of phytoene desaturase. © 2001 Society of Chemical Industry  相似文献   

Target strength estimation of black porgy Acanthopagrus schlegeli using acoustic measurements and a scattering model   总被引：1，自引：0，他引：1  

Donhyug  KANG  Kazuhiro  SADAYASU  Tohru  MUKAI  Kohji  IIDA  Doojin  HWANG  Kouichi  SAWADA  Kazushi  MIYASHITA 《Fisheries Science》2004,70(5):819-828

ABSTRACT:   Black porgy are hatched and released in marine ranching areas in Japan, Korea and Taiwan to maintain fisheries resources in coastal areas. If acoustic methods are used for field surveys, target strength ( TS ) information on the species is needed to convert acoustic data to biomass density. Target strength of black porgy ( Acanthopagrus schlegeli ) 15.5–32.9 cm fork length (nine individuals) was measured at 38 and 120 kHz (split beam) by ex situ measurements (cage and fishhook methods) of living fish, a tethered method with shock-frozen fish, and an acoustic scattering model, the Kirchhoff ray mode (KRM) model based on fish morphology. The ex situ TS -to-fork length ( FL , cm) relationships were TS _38kHz = 20log₁₀ FL  − 64.6 ( r  = 0.77) and TS _120kHz = 20log₁₀ FL  − 65.2 ( r  = 0.70). The values for the KRM model were TS _38kHz = 20log₁₀ FL  − 64.8 ( r  = 0.88) and TS _120kHz = 20log₁₀ FL  − 65.4 ( r  = 0.80). The agreement between the ex situ measurements and the KRM model for the mean TS was very good. In contrast, the results for the tethered method ( TS _38kHz = 20log₁₀ FL  − 69.3 [ r  = 0.42]) differed markedly from the other two methods, perhaps because of the effects of the shock-freezing procedure in air. The results of the ex situ measurements and scattering model provide basic information for studying TS and conducting acoustic surveys of black porgy, even though the tethered method must be modified.  相似文献