Seroepidemiologic studies on Babesia caballi and Babesia equi infections in Japan     

Ikadai H  Nagai A  Xuan X  Igarashi I  Tsugihiko K  Tsuji N  Oyamada T  Suzuki N  Fujisaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(4):325-328

Antibodies to Babesia caballi and Babesia equi were examined on a total of 2,019 horse serum samples that had been collected in 1971-1973 by the National Institute of Animal Health by enzyme-linked immunosorbent assay (ELISA) using recombinant proteins and by Western-blot analysis. Based on the criterion for positivity by ELISA, 5.4% (109/2,019) and 2.2% (44/2,019) had antibodies against B. caballi and B. equi, respectively. The ELISA-positive sera were further examined by Western blot; 30/109 for B. caballi and 2/ 44 for B. equi were positive for native B. caballi or B. equi, but none of them was seropositive for both infections. Based on the results of this study, further investigations should be required to survey horses that have arrived in Japan relatively recently and tick vectors of equine Babesia using ELISA with some recombinant protein, a parasite detection method in an in vitro culture of equine Babesia, and PCR testing.  相似文献   

Phenotype and function of murine peritoneal cavity macrophage derived-dendritic cells   总被引：4，自引：0，他引：4  

Makala LH  Nishikawa Y  Mishima M  Inoue N  Xuan X  Suzuki H  Fujisaki K  Mikami T  Nagasawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(9):813-820

The accessory activity was reported in murine peritoneal cavity macrophage derived dendritic cells (PEC-DC) in a mixed lymphocyte reaction (MLR). Here we continue the characterization of the generated PEC-DC using the criteria of morphology, phenotype and other accessory function. We have demonstrated that murine peritoneal cavity macrophages can be induced to differentiate in vitro into cells exhibiting typical dendritic cell (DC) morphology, phenotype and function. The proliferative capacity of the progenitors was amplified in the first step of the culture (day 0-7) using a combination of early cytokines: interleukin 4 and granulocyte-macrophage colony-stimulating factor. The second step of the culture started at day 7 with the removal of early growth factors to allow differentiation and final maturation of DC during 2 days of culture with interferon gamma plus either Toxoplasma lysate antigen (TLA) or lipopolysaccharide (LPS), a bacterial agent as a DC maturing agent. The resulting DC population exhibited typical DC morphology and expressed higher levels of MHC class II and the co-stimulatory molecules CD80 and CD86 compared to the untreated peritoneal cells. The generated DC cells efficiently presented soluble protein antigen to CD3(+) spleen T cells.  相似文献   

Clinical improvement in two dogs with hydrocephalus and syringohydromyelia after ventriculoperitoneal shunting     

Kitagawa M  Ueno H  Watanabe S  Igarashi O  Uzuka Y  Kanayama K  Sakai T 《Australian veterinary journal》2008,86(1-2):36-42

Two young Miniature Dachshunds were presented with abnormal gait. Magnetic resonance imaging showed, hydrocephalus with expanding fourth ventricle, and syringohydromyelia in the cervical spinal cord. These dogs underwent ventricle-peritoneal shunting, after which hydrocephalus, syringohydromyelia, and their clinical signs, improved.  相似文献   

Gene silencing of ribosomal protein P0 is lethal to the tick Haemaphysalis longicornis     

Gong H  Liao M  Zhou J  Hatta T  Huang P  Zhang G  Kanuka H  Nishikawa Y  Xuan X  Fujisaki K 《Veterinary parasitology》2008,151(2-4):268-278

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Molecular characterization of Theileria orientalis piroplasm protein encoded by an open reading frame (To ORF2) in a genomic fragment     

Kim JY  Yokoyama N  Kumar S  Inoue N  Fujisaki K  Sugimoto C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):957-963

In the present study, a novel antigenic protein expressed in the piroplasm stage of Theileria orientalis was characterized. A 4,707 bp genomic fragment amplified by PCR contained two open reading frames (ORFs). The deduced amino acid sequence of the first ORF showed significantly high similarlity to the ubiquitin carboxy terminal hydrolases/proteases while the second ORF (To ORF2) showed homology to several surface antigens of plasmodia. To ORF2 was expressed to determine whether the protein product is expressed by the parasite. In western blot analysis, bovine antiserum from a T. orientalis-infected calf recognized the recombinant protein containing a C-terminal part of the ORF expressed by baculovirus system. Western blot analysis with the anti-To ORF2 mouse serum recognized a 48 kDa protein in T. orientalis piroplasm lysates. Indirect immunofluorescence antibody test by confocal scanning laser microscopic analysis showed that antisera against the recombinant protein recognized T. orientalis piroplasm in the infected erythrocyte. The results from this study indicate that To ORF2 protein is expressed at the piroplasm stage and is immunogenic. This novel antigenic To ORF2 protein could be exploited for vaccine development against bovine piroplasmosis.  相似文献   

Cerebellar focal granulomatous meningoencephalitis in a dog: clinical findings and MR imaging     

Kitagawa M  Kanayama K  Satoh T  Sakai T 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2004,51(6):277-279

The authors encountered a dachshund dog, presenting vestibular disorder. On magnetic resonance (MR) imaging, a mass showing isointensity on the T1- and T2-weighted images and enhanced by contrast medium, was observed in the right cerebellum. In addition, the periphery of the mass showed isointensity on the T1-weighted image and hyperintensity on the T2-weighted image, suggesting sever oedema. Although the dog underwent surgery, it died. The mass was diagnosed pathologically as a granulomatous meningoencephalitis.  相似文献   

Effect of Litter Moisture on the Development of Footpad Dermatitis in Broiler Chickens     

Kazuyo TAIRA  Toshimune NAGAI  Takeshi OBI  Kozo TAKASE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):583-586

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Isolation and identification of indigestible pyroglutamyl peptides in an enzymatic hydrolysate of wheat gluten prepared on an industrial scale     

Higaki-Sato N  Sato K  Esumi Y  Okumura T  Yoshikawa H  Tanaka-Kuwajima C  Kurata A  Kotaru M  Kawabata M  Nakamura Y  Ohtsuki K 《Journal of agricultural and food chemistry》2003,51(1):8-13

An enzymatic hydrolysate of wheat gluten was further digested in vitro with porcine pepsin and pancreatin to obtain an indigestible peptide. Indigestible pyroglutamyl peptide was isolated from the digest by strong cation-exchange, size-exclusion, and reversed-phase chromatographies. The pyroglutamyl peptide was digested with pyroglutamate aminopeptidase, and the digest was reacted with phenyl isothiocyanate. The resultant phenylthiocarbamyl (PTC) peptides were purified by reversed-phase HPLC by using binary gradient elution with ammonium acetate buffer, pH 6.0, and acetonitrile. The PTC peptides were analyzed with an automatic peptide sequencer on the basis of the Edman degradation method with a modified program. Some pyroglutamyl peptides were also analyzed by fast-atom bombardment ionization mass spectrometry without the pyroglutamate amino peptidase digestion. Consequently, pyroGlu-Asn-Pro-Gln, pyroGlu-Gln-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gly-Gln-Gly-Gln, pyroGlu-Gln, pyroGlu-Gln-Pro, pyroGlu-Ile-Pro-Gln, pyroGlu-Ile-Pro, pyroGlu-Gln-Pro-Leu, pyroGlu-Gln-Phe-Pro-Gln, pyroGlu-Ser-Phe-Pro-Gln, pyroGlu-Phe-Pro-Gln, and pyroGlu-Gln-Pro-Pro-Phe-Ser were identified.  相似文献   

Characterization of <Emphasis Type="Italic">Inago1</Emphasis> and <Emphasis Type="Italic">Inago2</Emphasis> retrotransposons in <Emphasis Type="Italic">Magnaporthe oryzae</Emphasis>     

Edmundo SanchezJr.  Kozo Asano  Teruo Sone 《Journal of General Plant Pathology》2011,77(4):239-242

From the genome of a Japanese field isolate of the rice blast fungus, Magnaporthe oryzae, we newly identified Inago1 and Inago2 LTR retrotransposons. Both elements were found to be Ty3/gypsy-like elements whose copies were dispersed within the genome of Magnaporthe spp. isolates infecting rice and other monocot plants. Southern hybridization patterns of nine re-isolates derived from conidia of the strain Ina168 produced after a methyl viologen treatment were not changed, indicating that the insertion pattern of Inago elements is relatively stable.  相似文献   

Analysis of phenolic compounds in white rice, brown rice, and germinated brown rice   总被引：1，自引：0，他引：1  

Tian S  Nakamura K  Kayahara H 《Journal of agricultural and food chemistry》2004,52(15):4808-4813

Two hydroxycinnamate sucrose esters, 6'-O-(E)-feruloylsucrose and 6'-O-(E)-sinapoylsucrose, were isolated from methanol extracts of rice bran. Soluble and insoluble phenolic compounds as well as 6'-O-(E)-feruloylsucrose and 6'-O-(E)-sinapoylsucrose from white rice, brown rice, and germinated brown rice were analyzed using HPLC. The results demonstrated that the content of insoluble phenolic compounds was significantly higher than that of soluble phenolics in rice, whereas almost all compounds identified in germinated brown rice and brown rice were more abundant than those in white rice. 6'-O-(E)-Feruloylsucrose (1.09 mg/100 g of flour) and 6'-O-(E)-sinapoylsucrose (0.41 mg/100 g of flour) were found to be the major soluble phenolic compounds in brown rice. During germination, an approximately 70% decrease was observed in the content of the two hydroxycinnamate sucrose esters, whereas free phenolic acid content increased significantly; the ferulic acid content of brown rice (0.32 mg/100 g of flour) increased to 0.48 mg/100 g of flour and became the most abundant phenolic compound in germinated brown rice. The content of sinapinic acid increased to 0.21 mg/100 g of flour, which is nearly 10 times as much as that in brown rice (0.02 mg/100 g of flour). In addition, the total content of insoluble phenolic compounds increased from 18.47 mg/100 g of flour in brown rice to 24.78 mg/100 g of flour in germinated brown rice. These data suggest that appropriate germination of brown rice may be a method to improve health-related benefits.  相似文献