Effect of hCG Treatment on the Oestrous and Ovulation Responses to FSH in Prepubertal Gilts     

R Manjarin  JC Dominguez  MJ Castro  DJ Sprecher  G Cassar  RM Friendship  RN Kirkwood 《Reproduction in domestic animals》2009,44(3):432-434

To ensure sufficient numbers of pregnant females, particularly at hotter times of the year, hormonal induction of gilt oestrus may be necessary. However, the gilt oestrus and ovulation responses to gonadotrophin treatment have often proven unpredictable. The objective of this study was to examine possible reasons for this unpredictability. Prepubertal gilts (approximately 150 days of age, n = 63) were assigned to one of three treatments: injection of 300 IU hCG (n = 15); pre-treatment with 100 mg FSH in polyvinylpyrrolidinone administered as 2 × 50 mg injections 24 h apart, followed by 600 IU eCG at 24 h after the second FSH injection (n = 23); or FSH pre-treatment as above followed by 300 IU hCG at 24 h after the second FSH injection (n = 25). To facilitate oestrus detection, gilts were exposed to a mature boar for 15 min daily for 7 days. Blood samples were obtained on the day of eCG or hCG injection and again 10 days later and gilt ovulation responses determined based on elevated progesterone concentrations. The oestrus responses by 7 days were 6.7%, 17.5% and 64.0% for gilts treated with hCG, FSH + eCG and FSH + hCG, respectively (p < 0.001). The oestrous gilt receiving hCG alone and one oestrous FSH + hCG gilt did not ovulate, all other oestrous gilts ovulated. A further two anoestrous FSH + eCG-treated gilts ovulated. These data suggest that FSH pre-treatment facilitated the development of ovarian follicles to the point where they became responsive to hCG, but had little effect on the response to eCG.  相似文献   

Undifferentiated embryonic cell transcription factor 1 (UTF1) and deleted in azoospermia‐like (DAZL) expression in the testes of donkeys          下载免费PDF全文

YS Lee  HJ Jung  MJ Yoon 《Reproduction in domestic animals》2017,52(2):264-269

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Duration of serological response to canine parvovirus‐type 2, canine distemper virus,canine adenovirus type 1 and canine parainfluenza virus in client‐owned dogs in Australia     

SA Mitchell  RJ Zwijnenberg  J Huang  A Hodge  MJ Day 《Australian veterinary journal》2012,90(12):468-473

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An immunohistochemical study of canine disseminated aspergillosis     

MJ DAY  WJ PENHALE 《Australian veterinary journal》1991,68(12):383-386

An immunohistochemical study of 25 lesions from 7 dogs with disseminated aspergillosis (Aspergillus terreus) is presented. All had multiple fungal granulomas in many viscera, with centres of necrotic tissue and hyphal elements surrounded by a mixed infiltrate of predominantly mononuclear cells. Within these lesions, hyphae coated with immunoglobulin (IgG, IgM, IgA) and complement (C3, C4) were identified, together with peri-lesional mononuclear cells that reacted with antisera directed towards either IgG, IgM, IgA or a T lymphocyte marker (MUII). A conspicuous feature was the prominent hyphal fluorescence seen with IgA and C3 antisera. The IgA reagent also marked large numbers of mononuclear cells both around lesions and scattered throughout interstitial tissue, suggesting an abnormality of IgA production or regulation as a factor predisposing to this condition.  相似文献   

Intraperitoneal inoculation of ewes with an autogenous vaccine to prevent mastitis due to Pasteurella haemolytica     

MJ. KABAY  TM. ELLIS 《Australian veterinary journal》1989,66(10):342-343

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The pathogenesis of the nervous syndrome of Phalaris aquatica toxicity in sheep   总被引：1，自引：0，他引：1  

CA BOURKE  MJ CARRIGAN  RJ DIXON† 《Australian veterinary journal》1990,67(10):356-358

The clinical signs displayed by 96 sheep affected by the nervous syndrome of Phalaris aquatica toxicity and 10 normal sheep injected intravenously with the phalaris alkaloid, 5-methoxy dimethyltryptamine (dose range 0.01 to 5.0 mg/kg), were observed. The distributions of phalaris indole-like cytoplasmic pigments in nuclei of the brains and spinal cords of 9 naturally affected sheep were determined microscopically. Based on the relationship between clinical signs and the central nervous system nuclei involved in their production, the distribution of phalaris indole-like pigments, and the pharmacology of dimethylated tryptamines, it is suggested that the nervous syndrome induced by Phalaris aquatica results from a direct action of phalaris alkaloids upon serotonergic receptors in specific brain and spinal cord nuclei.  相似文献   

Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus)          下载免费PDF全文

MJ Sánchez‐Calabuig  C López‐Fernández  SD Johnston  D Blyde  J Cooper  K Harrison  J de la Fuente  J Gosálvez 《Reproduction in domestic animals》2015,50(2):227-235

Sperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species‐specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA fragmentation dynamics in both fresh and post‐thaw bottlenose dolphin sperm using the SCDt following different cryopreservation protocols to gain new information about the post‐thaw differential sperm DNA longevity in this species. Fresh and cryopreserved semen samples from five bottlenose dolphins were examined for sperm DNA fragmentation dynamics using the SCDt (Halomax^®). Sperm DNA fragmentation was assessed immediately at collection and following cryopreservation (T0) and then after 0.5, 1, 4, 8, 24, 48 and 72 h incubation at 37°C. Serially collected ejaculates from four dolphins were frozen using different cryopreservation protocols in a TES‐TRIS‐fructose buffer (TTF), an egg‐yolk‐free vegetable lipid LP1 buffer (LP1) and human sperm preservation medium (HSPM). Fresh ejaculated spermatozoa initially showed low levels of DNA fragmentation for up to 48 h. Lower Sperm DNA fragmentation (SDF) was found in the second fresh ejaculate compared to the first when more than one sample was collected on the same day (p < 0.05); this difference was not apparent in any other seminal characteristic. While there was no difference observed in SDF between fresh and frozen–thawed sperm using the different cryopreservation protocols immediately after thawing (T0), frozen–thawed spermatozoa incubated at 37°C showed an increase in the rate of SDF after 24 h. Sperm frozen in the LP1^? buffer had higher levels (p < 0.05) of DNA fragmentation after 24‐ and 48‐h incubation than those frozen in TTF or HSPM. No correlation was found between any seminal characteristic and DNA fragmentation in either fresh and/or frozen–thawed samples.  相似文献   

Refractory otitis media and otitis externa in a koala (Phascolarctos cinereus) treated with total ear canal ablation and lateral bulla osteotomy     

MJ Swaffield  M Liberts  AK Gillett  PA Moses 《Australian veterinary journal》2023,101(11):440-444

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Book Review     

MJ Lancaster 《Australian veterinary journal》1999,77(6):387-387

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The Solar Acoustic Spectrum and Eigenmode Parameters     

F Hill  PB Stark  RT Stebbins  ER Anderson  HM Antia  TM Brown  TL Duvall  DA Haber  JW Harvey  DH Hathaway  R Howe  RP Hubbard  HP Jones  JR Kennedy  SG Korzennik  AG Kosovichev  JW Leibacher  KG Libbrecht  JA Pintar  EJ Rhodes  J Schou  MJ Thompson  S Tomczyk  CG Toner  R Toussaint  WE Williams 《Science (New York, N.Y.)》1996,272(5266):1292-1296

The Global Oscillation Network Group (GONG) project estimates the frequencies, amplitudes, and linewidths of more than 250,000 acoustic resonances of the sun from data sets lasting 36 days. The frequency resolution of a single data set is 0.321 microhertz. For frequencies averaged over the azimuthal order m, the median formal error is 0.044 microhertz, and the associated median fractional error is 1.6 x 10(-5). For a 3-year data set, the fractional error is expected to be 3 x 10(-6). The GONG m-averaged frequency measurements differ from other helioseismic data sets by 0.03 to 0.08 microhertz. The differences arise from a combination of systematic errors, random errors, and possible changes in solar structure.  相似文献