Post-transplant crown allometry and shoot growth of two species of street trees     

Anu Riikonen  Leena Lindn  Minna Pulkkinen  Eero Nikinmaa 《Urban Forestry & Urban Greening》2011,10(2):87

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Parental eating attitudes and indicators of healthy eating in a longitudinal randomized dietary intervention trial (the STRIP study)     

Talvia S  Räsänen L  Lagström H  Anglè S  Hakanen M  Aromaa M  Sillanmäki L  Saarinen M  Simell O 《Public health nutrition》2011,14(11):2065-2073

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Factors causing variation in fine root biomass in forest ecosystems   总被引：4，自引：0，他引：4  

Leena Finér  Mizue Ohashi 《Forest Ecology and Management》2011,261(2):265-277

Fine roots form one of the most significant components contributing to carbon cycling in forest ecosystems. We study here the effect of variation in root diameter classes, sampling depth and the inclusion of understorey vegetation root biomass in fine root biomass (FRB) estimates. The FRB estimates for different forest biomes are updated using a database of 512 forest stands compiled from the literature. We also investigate the relationships between environmental or forest stand variables and fine root biomass (≤2 mm in diameter) at the stand (g m⁻²) and tree level (g tree⁻¹). The FRB estimates extrapolated for the whole rooting depth were 526 ± 321 g m⁻², 775 ± 474 g m⁻² and 776 ± 518 g m⁻² for boreal, temperate and tropical forests, respectively, and were 26-67% higher than those based on the original sampling depths used. We found significant positive correlations between ≤1 and ≤2 mm diameter roots and between ≤2 and ≤5 mm roots. The FRB estimates, standardized to the ≤2 mm diameter class, were 34-60% higher and 25-29% smaller than those standardized to the ≤1 mm and ≤5 mm diameter classes, respectively. The FRB of the understorey vegetation accounted for 31% of the total FRB in boreal forests and 20% in temperate forests. The results indicate that environmental factors (latitude, mean annual precipitation, elevation, temperature) or forest stand factors (life form, age, basal area, density) can not explain a significant amount of the variation in the total FRB and a maximum of 30% that in the FRB of trees at the stand level, whereas the mean basal area of the forest stand can explain 49% of the total FRB and 79% of the FRB of trees at the tree level.  相似文献   

Acute phase response in two consecutive experimentally induced E. coli intramammary infections in dairy cows     

Leena Suojala  Toomas Orro  Hanna J?rvinen  Johanna Saatsi  Satu Py?r?l? 《Acta veterinaria Scandinavica》2008,50(1):18

Background

Acute phase proteins haptoglobin (Hp), serum amyloid A (SAA) and lipopolysaccharide binding protein (LBP) have suggested to be suitable inflammatory markers for bovine mastitis. The aim of the study was to investigate acute phase markers along with clinical parameters in two consecutive intramammary challenges with Escherichia coli and to evaluate the possible carry-over effect when same animals are used in an experimental model.

Methods

Mastitis was induced with a dose of 1500 cfu of E. coli in one quarter of six cows and inoculation repeated in another quarter after an interval of 14 days. Concentrations of acute phase proteins haptoglobin (Hp), serum amyloid A (SAA) and lipopolysaccharide binding protein (LBP) were determined in serum and milk.

Results

In both challenges all cows became infected and developed clinical mastitis within 12 hours of inoculation. Clinical disease and acute phase response was generally milder in the second challenge. Concentrations of SAA in milk started to increase 12 hours after inoculation and peaked at 60 hours after the first challenge and at 44 hours after the second challenge. Concentrations of SAA in serum increased more slowly and peaked at the same times as in milk; concentrations in serum were about one third of those in milk. Hp started to increase in milk similarly and peaked at 36–44 hours. In serum, the concentration of Hp peaked at 60–68 hours and was twice as high as in milk. LBP concentrations in milk and serum started to increase after 12 hours and peaked at 36 hours, being higher in milk. The concentrations of acute phase proteins in serum and milk in the E. coli infection model were much higher than those recorded in experiments using Gram-positive pathogens, indicating the severe inflammation induced by E. coli.

Conclusion

Acute phase proteins would be useful parameters as mastitis indicators and to assess the severity of mastitis. If repeated experimental intramammary induction of the same animals with E. coli is used in cross-over studies, the interval between challenges should be longer than 2 weeks, due to the carry-over effect from the first infection.  相似文献   

Brook Water Quality and Background Leaching from Unmanaged Forested Catchments in Finland     

Mattsson  Tuija  Finér  Leena  Kortelainen  Pirkko  Sallantaus  Tapani 《Water, air, and soil pollution》2003,147(1-4):275-298

The water chemistry of 21 outlet brooks in undisturbed, forested catchments (0.074–38 km²) in Finland was monitored during three years (1997–1999) in order to estimate the background levels and leaching of total organic carbon (TOC), iron, nitrogen and phosphorus, and examine the relationships between catchment characteristics and brook water quality in pristine forested areas. The studied catchments are located throughout Finland except the northernmost parts of the country. The concentrations of TOC were relatively high, on average 20 mg L^-1. Annual leaching of TOC ranged from 3000 to 10 000 kg km^-2. The average total nitrogen concentration and annual leaching were 430 μg L^-1 and 140 kg km^-2, respectively. The average total phosphorus concentration and annual leaching were 15 μg L^-1 and 5.4 kg km^-2, respectively. On a national level temperature and discharge conditions were the most important variables for predicting total phosphorus, total nitrogen and TOC concentrations. Both total nitrogen and total phosphorus concentrationsdisplayed positive correlation with temperature, and thus the concentrations were higher in the south than in the north. Nitrogen was mainly organic and showed strong correlation with TOC. On the catchment level, both TOC and total nitrogen concentrations and export had a strongpositive correlation with the abundance of Norway spruce (Piceaabies Karsten) and a strong negative correlation with the abundance of Scots pine (Pinus sylvestris L.). Nitrate concentrations and leaching were related to average site type. The more fertile the average site type was in the catchment, the higher the nitrate concentrations and export were.  相似文献   

Forest edge structure as a shaping factor of understorey vegetation in urban forests in Finland     

Leena Hamberg  Susanna Lehvävirta  D. Johan Kotze 《Forest Ecology and Management》2009

We investigated the effects of edge structure (i.e. side-canopy openness based on tree, sapling and shrub characteristics, and the composition of tree species) on the understorey vegetation at mesic urban conifer-dominated forest edges in southern Finland. Forest edge structure had an effect on understorey vegetation, and on the spatial extent of the edge effect into the forests. At open edges the edge effect (in terms of the abundances of understorey vegetation) penetrated at least up to 30 m into the forest patches whereas closed edges may prevent these effects. A multilayered canopy with saplings and shrubs at the edge is important to alleviate the effects of the edge. We found that 225–250 m³ ha⁻¹ of trees (diameter at breast height (dbh) > 5 cm) is adequate to restrict the edge effect near the edge. However, the number of broad-leaved trees may be high at edges which, in turn, diminishes the abundance of mosses and favours herb species, thus changing the original natural understorey vegetation composition. Therefore we recommend that conifers be favoured at the edges of mesic conifer-dominated forest patches if the purpose is to restrict the extent of the effects of habitat edges. The appropriate proportion of conifers at these edges should be 80% or more.  相似文献   

Carbon sequestration in the growing stock of trees in Finland under different cutting and climate scenarios     

Juho Matala  Leena Kärkkäinen  Kari Härkönen  Seppo Kellomäki  Tuula Nuutinen 《European Journal of Forest Research》2009,128(5):493-504

In this work the aim was to determine how carbon sequestration in the growing stock of trees in Finland is dependent on the forest management and increased production potential due to climate change. This was analysed for the period 2003–2053 using forest inventory data and the forestry model MELA. Four combinations of two climate change and two management scenarios were studied: current (CU) and gradually warming (CC) climate and forest management strategies corresponding to different rates of utilisation of the cutting potential, namely maximum sustainable removal (Sust) or maximum net present value (NPV) of wood production (Max). In this analysis of Finland, the initial amount of carbon in the growing stock was 765 Mt (2,802 Tg CO₂). At the end of the simulation, the carbon in the growing stock of trees in Finland had increased to 894 Mt (3,275 Tg CO₂) under CUSust, 906 Mt (3,321 Tg CO₂) under CUMax, 1,060 Mt (3,885 Tg CO₂) under CCSust and 1,026 Mt (3,758 Tg CO₂) under CCMax. The results show that future development of carbon in the growing stock is not only dependent on climate change scenarios but also on forest management. For example, maximising the NPV of wood production without sustainability constraints results, over the short term, in a large amount of wood obtained in regeneration cuttings and a consequent decrease in the amount of carbon in growing stock. Over the longer term, this decrease in the carbon of growing stock in regenerated forests is compensated by the subsequent increase in fast-growing young forests. By comparison, no drastic short-term decrease in carbon stock was found in the Sust scenarios; only minor decreases were observed.  相似文献   

Physiological responses to continuous swimming in wild salmon (Salmo salar L.) parr and smolt     

Erkki Virtanen  Leena Forsman 《Fish physiology and biochemistry》1987,4(3):157-163

Wild salmon (Salmo salar) parr and smolt were forced to swim against constant flow (50 cm.s^–1) for 8 hours. Physiological properties describing the hormonal status, the energy metabolism and the ionic and osmotic balance of fish were measured from the fish prior to and at the end of the swimming test.Plasma cortisol levels were elevated in response to enforced swimming; the response of the smolt was clearly greater than that of the parr. Plasma thyroxine concentration increased in the parr but stayed at the initial level in the smolts. The parr consumed much of their coelomic fat, but the glycogen stores stayed nearly constant. The smolts had very low fat stores, and the glycogen stores were depleted in the test. The ionic and osmotic balance of the parr was stable in the test, but in smolts, the plasma Cl^–1 and osmotic concentrations decreased and muscle moisture increased.The results indicate that downstream migration smolts have markedly lower physiological capacity for continuous swimming than parr.  相似文献   

Transgenic barley by particle bombardment. Inheritance of the transferred gene and characteristics of transgenic barley plants     

Anneli Ritala  Reino Aikasalo  Kristian Aspegren  Marjatta Salmenkallio-Marttila  Satu Akerman  Leena Mannonen  Ulrika Kurtén  Riitta Puupponen-Pimiä  Teemu H. Teeri  Veli Kauppinen 《Euphytica》1955,85(1-3):81-88

Summary Transgenic barley plants (Hordeum vulgare L. cv. Kymppi) were obtained by particle bombardment of various tissues. Immature embryos and microspore-derived cultures were bombarded with gold particles coated with plasmid DNA carrying the gene coding for neomycin phosphotransferase II (NPTII), together with plasmid DNA containing the gene for -glucuronidase (GUS).Bombarded immature embryos were grown to plants without selection and NPTII activity was screened in small plantlets. One plant proved to be transgenic (T₀). This chimeric plant passed the transferred nptII gene to its T₁ progeny. The presence of the nptII gene was demonstrated by the PCR technique and enzyme activity was analyzed by an NPTII gel assay. Four T₀ spikes and 15 T₁ offspring were transgenic. The integration and inheritance was confirmed by Southern blot hybridization. Transgenic T₂ and T₃ plants were produced by isolating embryos from green grains of transgenic T₁ and T₂ plants, respectively and growing them to plants. After selfing, the ratio of transgenic to non-transgenic T₂ offspring was shown to follow the rule of Mendelian inheritance. The general performance of transgenic plants was normal and no reduction in fertility was observed.Microspore-derived cultures were bombarded one and four weeks after microspore isolation. After bombardment, cultures were grown either with or without antibiotic selection (geneticin ^R or kanamycin). When cultures were grown without selection and regenerated plants were transferred to kanamycin selection in rooting phase, one out of a total of about 1500 plants survived. This plant both carried and expressed the transferred nptII gene. The integration was confirmed by Southern blot hybridization. This plant was not fertile.  相似文献   

Seed set of ulluco (Ullucus tuberosus Loz.). Variation between clones and enhancement of seed production through the application of plant growth regulators     

Leena Pietilä  Paula Jokela 《Euphytica》1990,47(2):139-145

Summary Ulluco, Ullucus tuberosus, is a tuber crop plant with a reduced ability to produce botanical seed. This paper discusses problems related to the botanical seed set of ulluco. Ten clones of this species were studied, and the number of seeds per plant was recorded. Enhancement of seed production was attempted by the application of three growth regulators; gibberellic acid, CCC, and a synthetic cytokinin, 6-benzylamine purine. Seed set ability was shown to vary between the clones, and the application of plant growth regulators did not enhance seed production. Variation in fruit weight was observed. Because of genetic variation between clones in their ability to set seed, it is proposed that there is a possibility of breeding ulluco using botanical seeds. However, methods for improving the yield of botanical seed need to be developed.  相似文献