梨火疫细菌实时荧光PCR和诱捕PCR-ELISA检测方法的建立   总被引：8，自引：1，他引：8  

朱建裕  廖晓兰  高必达  朱水芳  陈红运  戴富泉 《植物检疫》2003,17(1):7-10

根据梨火疫细菌中独特而保守存在的质粒pEA29，设计了1对引物和3条探针，建立了实时荧光PCR检测方法和诱捕PCR-ELISA检测方法。实时荧光PCR采用带荧光标记的核酸杂交探针，边扩增边检测，步骤简单，不需PCR后处理，可避免假阳性和交叉污染；诱捕PCR-ELISA检测方法只需简单处理的样品就能检测，减少了核酸不纯出现的漏检，由于增加了核酸杂交探针，可不需凝胶电泳EB染色检测，不会出现假阳性问题。  相似文献   

低温胁迫下弓葵幼苗膜脂过氧化及保护酶活性的变化   总被引：32，自引：0，他引：32  

杨盛昌  谢潮添  张平  陈德海  丁印龙  廖启炓 《园艺学报》2003,30(1):104-106

 低温胁迫下弓葵( Butia capitata Becc) 幼苗叶片的MDA 含量逐渐增加, 膜脂过氧化作用增强。- 8 ℃条件下的膜脂过氧化作用明显强于2 ℃。细胞膜透性在2 ℃条件下变化不大, - 8 ℃时则随低温胁迫时间延长而急剧上升, 细胞膜受到伤害。- 8 ℃胁迫下细胞保护酶SOD、POD 和CAT 活性短期(6 h) 内升高,然后下降, 24 h 以后3 种保护酶受到低温胁迫的严重抑制。在2 ℃胁迫下, SOD 活性在6 h 内变化不大, 随后下降; CAT活性变化趋势与- 8 ℃时相似, 但变化幅度较小; POD 虽也呈现先升后降的趋势, 但降幅明显小于升幅, 至48 h 时POD 活性仍维持较高水平。2 ℃低温胁迫不是抑制而是促进POD 活性的提高。  相似文献   

小菊花器若干性状在F₁代的表现   总被引：11，自引：2，他引：11  

陈发棣  蒋甲福  郭维明 《园艺学报》2003,30(2):175-182

 以‘水晶白’、‘晚霞’等30个小菊品种为亲本，配制成28个杂交组合，对杂交后代的花器性状进行统计分析。结果发现，红色遗传能力大于黄色，且表现出较强的偏母性遗传特点，而原始色黄色和白色没有明显的偏母性遗传，以白色系品种作母本后代会出现许多亲本不具有的花色；杂交后代的舌状花数目、筒状花数目和花序直径与双亲比较，F，代表现出明显的遗传优势，杂种总平均舌状花数目、筒状花数目和花序直径分别相当于亲中值的122.6% 、128.7%、124.7% ，随着母本舌状花数目和筒状花数目的增大，杂种平均舌状花数目、筒状花数目和花序直径的增加幅度变缓，甚至出现衰弱的趋势，但花序直径除外。在花型上，平盘型的遗传能力比单窄瓣型的强。  相似文献   

Duplex PCR 快速检测松材线虫   总被引：2，自引：0，他引：2  

赵立荣  钟国强  廖金铃 《植物检疫》2004,18(6):324-326

利用duplex PCR技术对松材线虫(Bursaphelenchus xylophilus)与拟松材线虫(B. mucronatus)的rDNA部分核苷酸序列扩增.根据松材线虫与拟松材线虫的ITS1序列区别,设计出特异性引物,检测松材线虫的存在;在5.8S,28S保守序列区设计通用引物,检测伞滑刃线虫的存在.  相似文献   

奶牛隐性乳腺炎病原菌的分离鉴定     

林炜明  王轶敏  王志东  利光辉  靳亚平 《中国草食动物》2004,24(2):23-24

用兰州乳房炎试验(LMT)对来自西安市某个牛场的76份奶样作隐性乳房炎检验，并对乳样进行细菌分离鉴定。结果表明，该牛场的隐性乳房炎检出率为33％，阳性乳样中细菌检出率为93．4％。阴性乳样中病原菌检出率为28％，在检出的94株细菌中，共有5种菌22株是与乳房炎有关的病原菌。其中葡萄球菌和链球菌占病原菌总数的90％以上。是引起该场奶牛隐性乳房炎的主要病原菌。  相似文献   

Genetic variation in the MITF promoter affects skin colour and transcriptional activity in black-boned chickens     

G. Wang  J. Liao  M. Tang 《British poultry science》2018,59(1):21-27

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Effect of gut stress induced by oxidized wheat gluten on the growth performance,gut morphology and oxidative states of broilers          下载免费PDF全文

R. B. Liao  H. J. Yan  G. H. Liu  S. Zhang  W. H. Chang  W. Liu  C. H. Lin  X. Y. Huang  H. Y. Cai 《Journal of animal physiology and animal nutrition》2018,102(2):e849-e855

This study was to investigate the effect of oxidized wheat gluten (OG) on growth performance, gut morphology and its oxidative states of broilers. One hundred and eighty‐day‐old male broilers (10 chicks/pen) were randomly allocated into three dietary treatments: control diet (CON), diet with 8% wheat gluten (WG) and diet with 8% OG with six pens/treatment. Body weight (BW) (21 and 35 days) and average daily gain (ADG) (1–21 days and 22–35 days) decreased (p < .05) and feed conversion ratio (FCR) (1–21 days and 22–35 days) increased (p < .05) in OG treatment. Feed intake (FI) decreased (p < .05) in WG and OG treatments during 22–35 days. However, FI was not influenced by dietary treatments during 1–21 days (p > .05). The OG‐fed broilers had a lower faecal pH value (p < .05) and higher faecal moisture content (p < 05) at 14, 21, 28 and 35 days. Villus height, crypt depth and V/C value were not different (p > .05) among treatments at 21 and 35 days. Lipid peroxidation (LPO) (21 and 35 days) and malondialdehyde (MDA) (35 days) content in crop of OG treatment increased (p < .05). Oxidized glutathione (GSSG) (21 days), LPO (21 and 35 days) and MDA (21 and 35 days) content in ileum of OG treatment increased (p < .05). The reduced glutathione/oxidized glutathione (GSH/GSSG) (21 days) and (GSH) (35 days) in ileum of OG treatment decreased (p < .05). The present findings indicate that OG might be a stressor for broiler gut, which could induce oxidative stress both in crop and in ileum, and the diarrhoea as well. The growth performance of broiler was consequently depressed.  相似文献   

Effects of fat supplementation on plasma glucose,insulin and fatty acid analysis in ponies maintained on a forage‐based diet          下载免费PDF全文

T. Williams  B. Rude  S. Liao  C. Mochal‐King  M. Nicodemus 《Journal of animal physiology and animal nutrition》2018,102(4):1069-1076

The objective of this study was to observe how fat incorporated into an equine forage‐based diet through supplementation altered levels of plasma glucose, insulin and fatty acids. Five Shetland/Hackney cross pony mares were fed alfalfa pellet diets top dressed with commercially available vegetable oil (blend of soya bean, canola and corn oils) at 0%, 5%, 10% or 15% of diet. Ponies were randomly assigned one of four diets to start, with a 14‐day adjustment period between transitioning to another one of the four diets. Ponies were gradually adapted to the new diet within the 14‐day period before a five‐day trial period. Each pony received all four diets by the end of the study. Each trial was a five‐day period with a three‐day sample collection. Blood samples for each collection week were taken 0, 30, 60, 90, 120, 150, 180, 210, 240 and 270 min and at 5, 6, 7, 8, 9 and 10 hr post‐feeding. Excess fat did not impact plasma glucose (p > .1), nor did it affect blood plasma insulin concentration. While there was no time alteration found for plasma fatty acid concentration (p > .1), C14:0 increased when ponies were fed 0% fat and C18:2 decreased when ponies were fed 0% fat. Plasma fatty acids (% of total FA) were higher in C18:0, C18:1, C18:2 and C20:1 in the added fat diets (p < .1). These findings suggest the amounts reported in this study of fat supplementation on a forage‐based diet did influence the fatty acid analysis within the pony, but did not negatively impact blood glucose and insulin concentrations.  相似文献   

磷镁促释基质养分释放特性及种植效果     

张俊涛  叶少萍  廖宗文 《草业科学》2018,35(2):407-414

选用4种促释材料(W、Y、QW、FS)对磷矿粉、轻烧氧化镁混合物进行活化处理,配制促释材料含量分别为3%、5%的Ⅰ、Ⅱ型促释磷镁草花基质,通过淋溶试验、盆栽试验研究水溶性磷、镁素释放特性以及对夏堇(Torenia fournieri)、本地长春花(Catharanthus roseus)生长及开花的影响。结果显示,促释材料W、Y、QW、FS对磷矿粉均表现出良好的促释效果,磷镁促释基质水溶性磷淋溶总量较不添加促释磷镁显著增加了15.42%~27.10%(P0.05);添加4种促释材料对水溶性镁总量释放效果均不显著(P0.05)。促释磷镁W-Ⅰ不仅增加了夏堇开花数量,同时对本地长春花的冠幅增长、分枝以及地上部生物量积累有促进作用;FS-Ⅱ则有利于夏堇地上部生物量的积累;Y-Ⅰ则对夏堇、本地长春花生长无促进作用。综合考虑,草花基质添加促释磷镁W-Ⅰ、FS-Ⅱ应用效果最好,其最适添加比例分别为3.09、3.16 kg·m-3。  相似文献   

温室自动牵引车设计与应用          下载免费PDF全文

王志冉  周增产  姚涛  张晓文  马铁  曲维明 《农业工程》2020,10(4):27-31

自动导航牵引车在工业中担任运输物料的作用，随着单栋温室的面积不断增大，操作区之间的距离逐渐增大，为了实现各操作区之间的联系，高效完成运输、采摘和喷洒等各项任务，开发一种应用于温室中的自动导航牵引车。该设备主要包括驱动总成、转向总成、AGV控制总成、手动控制总成、路面清理装置、铅酸蓄电池、底盘和外壳。开发的温室自动牵引车不仅能够实现自动导航，在面积巨大、路况复杂或线路不确定的区域还能够实现手动控制。在温室生产中应用灵活方便，极大地提高了现代农业的装备化水平。   相似文献