Biochemical and molecular analysis of carotenoid biosynthesis in flavedo of orange (Citrus sinensis L.) during fruit development and maturation   总被引：8，自引：0，他引：8  

Rodrigo MJ  Marcos JF  Zacarías L 《Journal of agricultural and food chemistry》2004,52(22):6724-6731

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Spectroscopic detection of forest diseases:a review (1970-2020)     

Lorenzo Cotrozzi 《林业研究》2022,33(1):21-38

Sustainable forest management is essential to confront the detrimental impacts of diseases on forest eco-systems.This review highlights the potential of vegetation spectroscopy in improving the feasibility of assessing forest disturbances induced by diseases in a timely and cost-effec-tive manner.The basic concepts of vegetation spectroscopy and its application in phytopathology are first outlined then the literature on the topic is discussed.Using several opti-cal sensors from leaf to landscape-level,a number of for-est diseases characterized by variable pathogenic processes have been detected,identified and quantified in many coun-try sites worldwide.Overall,these reviewed studies have pointed out the green and red regions of the visible spec-trum,the red-edge and the early near-infrared as the spectral regions most sensitive to the disease development as they are mostly related to chlorophyll changes and symptom develop-ment.Late disease conditions particularly affect the short-wave-infrared region,mostly related to water content.This review also highlights some major issues to be addressed such as the need to explore other major forest diseases and geographic areas,to further develop hyperspectral sensors for early detection and discrimination of forest disturbances,to improve devices for remote sensing,to implement long-term monitoring,and to advance algorithms for exploitation of spectral data.Achieving of these goals will enhance the capability of vegetation spectroscopy in early detection of forest stress and in managing forest diseases.  相似文献   

Aspectos Cronologicos en la Maduracion Nuclear de Oocitos Bovinos Cultivados in vitro     

P. Lorenzo    M. J. Illera    J. C. Illera  M. Illera 《Anatomia, histologia, embryologia》1995,24(2):139-144

In this study, the chronological changes in the meiotic progress of in vitro maturation of bovine oocytes was analysed. Oocytes (n = 1044) were obtained from a local abbatoir and were classified into two groups according to the presence or absence of cumulus cells. They were incubated in microdrops (5 per drop) in TCM-199 (control), TCM-199 + 10% fetal calf serum (treatment 2) and TCM-199 + 10% estrous cow serum (treatment 3). Oocytes were fixed and stained at the end of 0, 6, 12, 18, 24 and 30h of the beginning of in vitro culture, were evaluated according to the nuclear stage of maturation. These results demonstrated that the immature oocytes at the time of collection (0 h) were in the germinal vesicle stage (GV), and that the highest maturation rate was at 24 h of culture in all treatments. Serum treatments enhanced the maturation rates obtained (52.1 and 55.7%) compared to control (serum-free) medium (42.7%; P<0.05) in cumulus-cell-enclosed oocytes. In denuded oocytes, the maturation rates were lower compared to cumulus-cells-enclosed oocytes in all treatments. In conclusion, meiotic progression of bovine oocytes can be influenced by the inclusion of sera in the maturation media and by the presence of the cumulus cells.  相似文献   

Sampling Shrimp Populations in Hatcheries     

Lorenzo M.  Juarez Allan H.  Luxem  David B.  Rouse 《Journal of the World Aquaculture Society》1996,27(2):218-222

Abstract.– Shrimp populations were estimated with volumetric sampling techniques during different phases of hatchery operations. Factors considered which might effect precision of estimates included stage age, density and temperature. Estimates of nauplii were obtained with coefficient of variation values smaller than 3% when nauplii were concentrated in 20-L buckets and smaller than 8% when released in 13,000 L of water in larval rearing tanks. In both cases estimates were of sufficient precision to make management decisions for routine hatchery work. A technique was developed for estimating shrimp populations in larval rearing tanks with coefficient of variation values smaller than 15%. Volumetric sampling of postlarvae older than PL₄, in larval rearing tanks was highly variable due to the tendency of shrimp to congregate and attach to tank walls and bottoms. A direct relationship was observed between postlarval density and the precision of population estimates in harvest buckets. Water temperature and postlarval age did not affect precision at the densities and ages studied. These findings have important implications for commercial transactions. Industry standards utilizing coefficient of variation are recommended for larvae and postlarvae population estimates.  相似文献   

A PCR technique for the detection of Jaagsiekte sheep retrovirus in the blood suitable for the screening of ovine pulmonary adenocarcinoma in field conditions     

De Las Heras M  Ortín A  Salvatori D  Pérez de Villareal M  Cousens C  Miguel Ferrer L  Miguel Cebrián L  García de Jalón JA  Gonzalez L  Michael Sharp J 《Research in veterinary science》2005,79(3):201-264

Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring contagious lung neoplasia caused by jaagsiekte sheep retrovirus (JSRV). Although no specific circulating antibodies against the virus can be detected in infected sheep, JSRV proviral DNA sequences can be found in peripheral blood leukocytes (PBLs) in clinically affected and in a proportion of in contact animals. In this study, existing hemi-nested PCR procedure is compared with a new one-step PCR technique that was developed to minimise potential DNA contamination and reduce sample and reagent handling. Different blood preparations were assessed and the best results were achieved on DNA prepared from buffy coat. The sensitivity of this PCR was lower in JSRV infected sheep without lesions of OPA than in clinically affected sheep, which indicate that this PCR may not be not fully appropriate for screening of individual sheep, but rather to provide results at flock level. This PCR is the only currently available blood test for detection of JSRV infected sheep and may be useful in epidemiological studies and in control programmes of OPA.  相似文献   

Type A-like retroviral particles in a metastatic intestinal adenocarcinoma in an emerald tree boa (Corallus caninus)     

Orós J  Lorenzo H  Andrada M  Recuero J 《Veterinary pathology》2004,41(5):515-518

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Changes in the free amino acid contents of honeys during storage at ambient temperature     

Iglesias MT  Martín-Alvarez PJ  Polo MC  de Lorenzo C  Gonzalez M  Pueyo E 《Journal of agricultural and food chemistry》2006,54(24):9099-9104

This study was carried out to establish the changes in the free amino acid contents of floral honeys, honeydew honeys, and blend honeys during storage at room temperature and to test the capacity of the amino acids to distinguish the origin of the honeys after storage. For this purpose, 54 artisanal honeys (39 floral, 5 honeydew, and 10 blend) were studied. Samples were taken from recently collected honeys and at 3, 6, 9, 12, 16, 20, and 24 months after harvesting. The contents of most of the free amino acids were found to decrease with storage time, with the greatest reduction observed in the first 9 months. The contents of the amino acids aspartic acid, beta-alanine, and proline increased in the first few months after storage, reaching maximum values at 6 months, suggesting the possible existence of enzymatic activities. The application of stepwise discriminant analysis to the free amino acid content data demonstrated that the contents of the amino acids valine, beta-alanine, gamma-aminobutyric acid, serine, isoleucine, alpha-alanine, ornithine, and glutamine correctly assigned 87% of honeys to their group of origin: floral, honeydew, or blend.  相似文献   

Polar constituents from the aerial parts of Origanum vulgare L. Ssp. hirtum growing wild in Greece     

Koukoulitsa C  Karioti A  Bergonzi MC  Pescitelli G  Di Bari L  Skaltsa H 《Journal of agricultural and food chemistry》2006,54(15):5388-5392

From the polar extracts of Origanum vulgare L. ssp. hirtum 19 compounds have been isolated. The structures and relative stereochemistry have been elucidated by spectroscopic analysis and determined as apigenin, luteolin, chrysoeriol, diosmetin, quercetin, eriodictyol, cosmoside, vicenin-2, caffeic acid, p-menth-3-ene-1,2-diol 1-O-beta-glucopyranoside, thymoquinol 2-O-beta-glucopyranoside, thymoquinol 5-O-beta-glucopyranoside, thymoquinol 2,5-O-beta-diglucopyranoside, 12-hydroxyjasmonic acid, 12-hydroxyjasmonic acid 12-O-beta-glucopyranoside, lithospermic acid B, rosmarinic acid, 10-epi-lithospermic acid, and epi-lithospermic acid B. The three latter products display unusual stereochemistry of the 3,4-hydroxyphenyllactic acid unit(s), which to the authors' best knowledge has never been reported before in similar compounds. Moreover, lithospermic acid B (and its stereoisomers), p-menth-3-ene-1,2-diol 1-O-beta-glucopyranoside, 12-hydroxyjasmonic acid, and 12-hydroxyjasmonic acid 12-O-beta-glucopyranoside were isolated for the first time from Origanum species.  相似文献   

Control or eradication? Costs and benefits in the case of PRRSV     

Fraile L 《The Veterinary record》2012,170(9):223-224

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Correlation Between Catechin Content and NF-κB Inhibition by Infusions of Green and Black Tea     

Chiara Di Lorenzo  Mario Dell’Agli  Enrico Sangiovanni  Ariana Dos Santos  Francesca Uberti  Enzo Moro  Enrica Bosisio  Patrizia Restani 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(2):149-154

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