Fatty acid profile and qualitative characteristics of meat from zebu steers fed with different oilseeds     

Oliveira DM  Ladeira MM  Chizzotti ML  Machado Neto OR  Ramos EM  Gonçalves TM  Bassi MS  Lanna DP  Ribeiro JS 《Journal of animal science》2011,89(8):2546-2555

The objective of this study was to evaluate the effects of dietary addition of ground oilseed sources on the quality, fatty acid profile, and CLA content of meat from zebu steers. Thirty-one zebu steers with an initial average age of 23 mo and an initial BW of 365 kg were used in this study. The experimental period was 84 d, which was preceded by an adaption period of 28 d. The diet was provided ad libitum with a forage:concentrate ratio of 40:60. Corn silage was used as the forage source. Four different concentrates were formulated for each treatment: without additional lipids (control) or with ground soybeans (SB), ground cottonseed (CS), or ground linseed (LS). The SB, CS, and LS diets were formulated to have 6.5% ether extract on a total dietary DM basis. The experiment was set up as a completely randomized design. After slaughter, samples were taken from the longissimus thoracis muscle for the measurement of fatty acid concentration and the evaluation of meat quality. The luminosity index was greater in the control and LS diets (P < 0.01). The greatest percentages of myristic acid (C14:0), palmitic acid (C16:0), trans octadecenoic acid (C18:1 trans-10, trans-11, or trans-12), and SFA in the subcutaneous fat were observed in the CS treatment (P < 0.01). Moreover, the least percentages of oleic acid (C18:1 cis-9) and total unsaturated fatty acids in the subcutaneous fat were observed in the CS diet (P < 0.01). The meat linoleic acid and α-linolenic acid percentages were greatest in the SB and LS treatments, respectively (P < 0.001). The unsaturated fatty acid:SFA ratio was smallest for the CS diet (P < 0.01). A gradual increase in oxidation was observed as a function of storage time; however, the diets did not affect the rancidity of the meat (P > 0.05). The fatty acid profile of subcutaneous fat was impaired by the addition of CS. Supplying ground oilseeds did not increase the content of CLA in the meat.  相似文献   

What is your diagnosis? Lymphadenopathy in a cow with severe anemia     

Salgado BS  Battaglia CT  Stuchi RS  Cadioli FA  Rozza DB  Machado GF 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(1):103-104

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In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells     

Rondon FC  Bevilaqua CM  Accioly MP  Morais SM  Andrade-Junior HF  Machado LK  Cardoso RP  Almeida CA  Queiroz-Junior EM  Rodrigues AC 《Veterinary parasitology》2011,178(3-4):235-240

In South America, visceral leishmaniasis is a zoonosis caused by the protozoan species Leishmania infantum (syn. L. chagasi) and is primarily transmitted through the bite of the female Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommended by health agencies have not achieved significant results in reducing the incidence of human cases, and the lack of effective drugs to treat dogs resulted in the prohibition of this course of action in Brazil. Therefore, it is necessary to search new alternatives for the treatment of canine and human visceral leishmaniasis. The objectives of this study were to evaluate the in vitro effect of fractions from Aloe vera (aloe), Coriandrum sativum (coriander), and Ricinus communis (castor) on promastigotes and amastigotes of L. infantum and to analyze the toxicity against the murine monocytic cells RAW 264.7. To determine the viability of these substances on 50% parasites (IC50), we used a tetrazolium dye (MTT) colorimetric assay (bromide 3-4.5-dimethylthiazol-2-yl-2,5-dephenyltetrazolium), and on amastigotes we performed an in situ ELISA. All fractions were effective against L. infantum promastigotes and did not differ from the positive control pentamidine (p>0.05). However, the R. communis ethyl acetate and chloroform fractions, as well as the C. sativum methanol fraction, were the most effective against amastigotes and did not differ from the positive control amphotericin B (p>0.05). The R. communis ethyl acetate fraction was the least toxic, presenting 83.5% viability of RAW 264.7 cells, which was similar to the results obtained with amphotericin B (p>0.05). Based on these results, we intend to undertake in vivo studies with R. communis ethyl acetate fractions due the high effectiveness against amastigotes and promastigotes of L. infantum and the low cytotoxicity towards murine monocytic cells.  相似文献   

In silico structural characteristics and α-amylase inhibitory properties of Ric c 1 and Ric c 3, allergenic 2S albumins from Ricinus communis seeds     

Do Nascimento VV  Castro HC  Abreu PA  Oliveira AE  Fernandez JH  Araújo Jda S  Machado OL 《Journal of agricultural and food chemistry》2011,59(9):4814-4821

The major Ricinus communis allergens are the 2S albumins, Ric c 1 and Ric c 3. These proteins contain a trypsin/α-amylase inhibitor family domain, suggesting that they have a role in insect resistance. In this study, we verified that Ric c 1 and Ric c 3 inhibited the α-amylase activity of Callosobruchus maculatus, Zabrotes subfasciatus, and Tenebrio molitor (TMA) larvae as well as mammalian α-amylase. The toxicity of 2S albumin was determined through its incorporation in C. maculatus larvae as part of an artificial diet. Bioassays revealed that 2S albumin reduced larval growth by 20%. We also analyzed the tridimensional structures of Ric c 1 and Ric c 3 by (a) constructing a comparative model of Ric c 1 based on Ric c 3 NMR structure and (b) constructing the theoretical structure of the Ric c 1-TMA and Ric c 3-TMA complexes. Our biological and theoretical results revealed that Ric c 1 and Ric c 3 are a new class of α-amylase inhibitors. They could potentially be used to help design inhibitors that would be useful in diverse fields, ranging from diabetes treatment to crop protection.  相似文献   

Sex-related macrostructural organization of the deer's brachial plexus     

Melo SR  Gonçalves AF  de Castro Sasahara TH  Fioretto ET  Gerbasi SH  Machado MR  Guimarães GC  Ribeiro AA 《Anatomia, histologia, embryologia》2007,36(4):295-299

We describe the morphological organization of the deer brachial plexus in order to supply data to veterinary neuroclinics and anaesthesiology. The deer (Mazama gouazoubira) brachial plexus is composed of four roots: three cervical (C6, C7 and C8) and one thoracic (T1). Within each sex group, no variations are observed between the left and the right brachial plexus, though sex-related differences are seen especially in its origin. The origin of axillary and radial nerves was: C6, C7, C8 and T1 in males and C8-T1 (radial nerve) and C7, C8 and T1 (axillary nerve) in females; musculocutaneous nerve was: C6-C7 (males) and C8-T1 (females); median and ulnar nerves was: C8-T1 (males) and T1 (females); long thoracic nerve was: C7 (males) and T1 (females); lateral thoracic nerve was: C6, C7, C8 and T1 (males) and T1 (females); thoracodorsal nerve was: C6, C7, C8 and T1 (males) and C8-T1 (females); suprascapular nerve was: C6-C7 (males) and C6 (females) and subscapular nerve was: C6-C7 (males) and C7 (females). This study suggests that in male deer the origin of the brachial plexus is more cranial than in females and the origin of the brachial plexus is slightly more complex in males, i.e. there is an additional number of roots (from one to three). This sexual dimorphism may be related to specific biomechanical functions of the thoracic limb and electrophysiological studies may be needed to shed light on this morphological feature.  相似文献   

Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints     

Juliana J. Moreira  Ana Paula L. Moraes  Patrícia M. Brossi  Thaís S.L. Machado  Yara M. Michelacci  Cristina O. Massoco  Raquel Y.A. Baccarin 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):47-55

This experimental controlled study was performed to evaluate the composition of autologous processed plasma (APP), and the effects of APP intra-articular injection into healthy equine metacarpophalangeal joints. The effects on joints were analysed with a short-phase protocol and a prolonged-phase protocol using saline-injected joints as controls. For the short protocol, horses received one intra-articular APP injection. Synovial fluid samples were collected prior to the injection and 3, 6, 24, 48, and 16 h after treatment. For the prolonged protocol, the joints received three weekly injections of APP, and samples were collected at 0, 7, 14, 21, and 28 days before APP administration. IL1-ra level was found to be increased in APP compared to plasma. Upon intra-articular administration of APP, transient (up to 24 h) increases in white blood cell (WBC) counts along with elevated protein and prostaglandin E₂ (PGE₂) concentrations were observed in the treated joints. Over the 28-day observation period, APP did not elicit changes relative to baseline levels, but WBC counts, PGE₂ and chondroitin sulphate concentrations were lower than those found in the control. In conclusion, APP intra-articular injection induced a mild and transitory inflammatory response but no inflammation reaction was observed over a longer period of treatment and observation.  相似文献   

Tomato root distribution, yield and fruit quality under different subsurface drip irrigation regimes and depths   总被引：4，自引：0，他引：4  

Rui M. A. Machado  Maria do Rosàrio G. Oliveira 《Irrigation Science》2005,24(1):15-24

Tomato rooting patterns, yield and fruit quality were evaluated in a field trial where three irrigation regimes [0.6 (DI), 0.9 (DII) and 1.2 ET_c (DIII)] and three drip irrigation depths [surface (R0), subsurface at 20 cm depth (RI) and subsurface at 40 cm depth (RII)] were imposed following a split-plot experimental design, with four replications. The behaviour of the root system in response to the irrigation treatments was evaluated using minirhizotrons installed between two plants, near the plant row. Root-length intensity (L _a)—length of the root per unit of minirhizotron surface area (cm cm⁻²)—was measured at four crop stages. For all sampling dates, none of the factors studied were found to influence L _a or rooting depth significantly or the interaction between treatments. For all treatments most of the root system was concentrated in the top 40 cm of the soil profile, where the root-length density ranged from 0.5 cm cm⁻³ to 1.4 cm cm⁻³ . The response of tomato fruits to an increase in the water applied was similar in quantitative and qualitative terms for the different drip irrigation depths. Water applied by drip irrigation had the opposite effect on commercial yield (t ha⁻¹) and soluble solids (°Brix) (r=−0.82, P<0.001), however, yield in terms of total soluble solids (t ha⁻¹) was the same for the 0.9 and 1.2 ET_c. The increase in commercial yield can be described by the equation   相似文献   

Vasorelaxation, induced by Dictyota pulchella (Dictyotaceae), a brown alga, is mediated via inhibition of calcium influx in rats     

Queiroz TM  Machado NT  Furtado FF  Oliveira-Filho AA  Alustau MC  Figueiredo CS  Miranda GE  Barbosa-Filho JM  Braga VA  Medeiros IA 《Marine drugs》2011,9(10):2075-2088

This study aimed to investigate the cardiovascular effects elicited by Dictyota pulchella, a brown alga, using in vivo and in vitro approaches. In normotensive conscious rats, CH(2)Cl(2)/MeOH Extract (CME, 5, 10, 20 and 40 mg/kg) from Dictyota pulchella produced dose-dependent hypotension (-4 ± 1; -8 ± 2; -53 ± 8 and -63 ± 3 mmHg) and bradycardia (-8 ± 6; -17 ± 11; -257 ± 36 and -285 ± 27 b.p.m.). In addition, CME and Hexane/EtOAc Phase (HEP) (0.01-300 μg/mL) from Dictyota pulchella induced a concentration-dependent relaxation in phenylephrine (Phe, 1 μM)-pre-contracted mesenteric artery rings. The vasorelaxant effect was not modified by the removal of the vascular endothelium or pre-incubation with KCl (20 mM), tetraethylammonium (TEA, 3 mM) or tromboxane A(2) agonist U-46619 (100 nM). Furthermore, CME and HEP reversed CaCl(2)-induced vascular contractions. These results suggest that both CME and HEP act on the voltage-operated calcium channel in order to produce vasorelaxation. In addition, CME induced vasodilatation after the vessels have been pre-contracted with L-type Ca(2+) channel agonist (Bay K 8644, 200 nM). Taken together, our data show that CME induces hypotension and bradycardia in vivo and that both CME and HEP induce endothelium-independent vasodilatation in vitro that seems to involve the inhibition of the Ca(2+) influx through blockade of voltage-operated calcium channels.  相似文献   

Nitrogen,Phosphorus and Potassium Fertilization Interactions on the Photosynthesis of Containerized Citrus Nursery Trees     

Alberto C. De Campos Bernardi  Quirino A. De Camargo Carmello  Sérgio Alves De Carvalho  Eduardo Caruso Machado  Camilo Lázaro Medina  Mara De Menezes De Assis Gomes 《Journal of plant nutrition》2015,38(12):1902-1912

The objective of this research was to evaluate the nitrogen, phosphorus and potassium (NPK) fertilizer effect on the development of ‘Valencia’ sweet orange nursery trees budded on Rangpur lime rootstocks in a greenhouse using containers. The experiment was a complete (1/5) 5³ factorial randomized blocks design. Treatments comprised five concentrations (g per plant) of N (1.25; 6.25; 11.25; 16.25; 21.25); K (0.42; 3.75; 6.22; 9.34; 12.45); and P (0.19; 0.89; 1.59; 2.29; 2.99). Carbon dioxide assimilation rate (A), transpiration rate (E), stomata conductance (g_s), and internal carbon dioxide (CO₂) concentration (Ci) and water use efficiency (WUE). Reducing sugar, sucrose and starch were evaluated. Response functions were adjusted and nutrient rates for maximum yield were presented. The results indicated that the high levels of N (>16.25 g per plant) interferes negatively on photosynthesis. Potassium at intermediate fertilization levels (from 3.75 to 6.22 g per plant) had positive effects on photosynthesis. But P had little interference on photosynthesis. Carbohydrates levels were not related to nutrients fertilized.  相似文献   

Automatic flow system with voltammetric detection for diacetyl monitoring during brewing process     

Rodrigues PG  Rodrigues JA  Barros AA  Lapa RA  Lima JL  Machado Cruz JM  Ferreira AA 《Journal of agricultural and food chemistry》2002,50(13):3647-3653

Diacetyl can be determined by adsorptive stripping voltammetry after derivatization with o-phenylenediamine. The method may be applicable to the determination of diacetyl in different foods, being a good alternative to other analytical methods. In this work an on-line automated analytical system for diacetyl determination in beer is described. A hanging mercury drop electrode voltammetric flow detector was used, and the analyte was determined without the traditional deoxygenation procedure. The method was successfully applied to the determination of diacetyl during beer fermentation and in the final product. The automation strategy used was based on a flow network similar to those used in multicommutated flow systems, with a pervaporation unit used for diacetyl separation. The developed system was tested in real conditions in the monitoring of brewing processes. The results obtained were similar to those obtained with the usual GC-ECD methodology in the 5-600 ppb range. The analytical rate of the developed method is about 12 determinations/h.  相似文献