Correlation between α-amylase gene expression and seedling vigor in rice     

Erik E. Karrer  John M. Chandler  Majid R. Foolad  Raymond L. Rodriguez 《Euphytica》1992,66(3):163-169

Summary This report examines the relationship between seedling vigor, -amylase enzyme activity and -amylase mRNA accumulation in ten varieties of rice (Oryza sativa L.) grown at two temperatures (15°C and 30°C). A significant, positive correlation was observed between seedling vigor, -amylase enzyme activity, and the accumulation of mRNA from one rice -amylase gene (RAmy1A) at both temperature regimens. The results of this study support previous experiments which have correlated -amylase enzyme activity to seedling vigor. We have extended this correlation to the expression of one of ten genes that comprise the rice -amylase multigene family. These results suggest that the expression of -amylase gene RAmy1A is an important, and possibly rate-limiting factor in determining seedling vigor in rice.  相似文献   

Accumulation and remobilization of assimilates in different genotypes of durum wheat under terminal drought stress     

Vosoghi Rad  Mehdi  Jami Moeini  Matin  Taherian  Majid  Armin  Mohammad 《Journal of Crop Science and Biotechnology》2022,25(2):199-214

Journal of Crop Science and Biotechnology - The present research was conducted to examine the accumulation and remobilization of some promising lines of durum wheat assimilates using a randomized...  相似文献   

Acephalous lamb from an in vitro-produced sheep embryo          下载免费PDF全文

Abolfazl Shirazi  Ebrahim Ahmadi  Majid Jadidi  Naser Shams-Esfandabadi    Banafsheh Heidari 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(5):501-505

This is the first report of an acephalous lamb from the transfer of an in vitro-produced sheep embryo. Twelve in vitro-fertilized embryos were transferred to 4 recipient ewes (3 embryos/ewe). Two ewes remained pregnant: one delivered a normal female lamb, the other a male acephalous lamb. Possible contributing factors are discussed.  相似文献   

A Novel Splice Site Variant in the LDLRAP1 Gene Causes Familial Hypercholesterolemia     

Najmeh Ahangari  Amirhossein Sahebkar  Mohsen Azimi-Nezhad  Hamideh Ghazizadeh  Mohsen Moohebati  Mahmoud Ebrahimi  Habibollah Esmaeili  Gordon A. Ferns  Alireza Pasdar  Majid Ghayour Mobarhan 《Iranian Biomedical Journal》2021,25(5):374

Background:FH, a hereditary disorder, is caused by pathogenic variants in the LDLR, APOB, and PCSK9 genes. This study has assessed genetic variants in a family, clinically diagnosed with FH. Methods:A family was recruited from MASHAD study in Iran with possible FH based on the Simon Broom criteria. The DNA sample of an affected individual (proband) was analyzed using WES, followed by bioinformatics and segregation analyses. Results:A novel splice site variant (c.345-2A>G) was detected in the LDLRAP1 gene, which was segregated in all affected family members. Moreover, HMGCR rs3846662 g.23092A>G was found to be homozygous (G/G) in the proband, probably leading to reduced response to simvastatin and pravastatin. Conclusion:LDLRAP1 c.345-2A>G could alter the PTB, which acts as an important part of biological pathways related to lipid metabolism. Key Words: Genetic research, LDLRAP1, Hypercholesterolemia, Hydroxymethylglutaryl-CoA Reductase Inhibitors  相似文献   

Metallothionein biosynthesis as a detoxification mechanism in mercury exposure in fish,spotted scat (<Emphasis Type="Italic">Scatophagus argus</Emphasis>)     

Mahmood Sinaie  Kazem Darvish Bastami  Masoud Ghorbanpour  Hossein Najafzadeh  Majid Shekari  Sara Haghparast 《Fish physiology and biochemistry》2010,36(4):1235-1242

It is of crucial importance to study on the biomarkers types to assess the specification of the pollutants and health status of marine ecosystems in environmental evaluation projects. In this respect, total metallothionein biosynthesis and mercury bioaccumulation in the liver and gills under acute mercury exposure were investigated in fish, Scat (Scatophagus argus). Spotted scat was exposed to different mercury concentrations (0, 10, 20, 30) for 24, 48, 72 h. Total MT levels were determined by enzyme-linked immunosorbent assay (ELISA) method. Mercury contents were determined through cold vapor atomic absorption spectrometry (CVAAS). Induction of MT during exposure was tissue specific, displaying different response pattern in gills and liver. Mercury accumulated in liver much higher than in gills and the latter also showed lower MT level (P < 0.05). MT biosynthesis in liver showed a significant (P < 0.05) increase after exposure to different mercury concentration with increase in exposure time, whereas total MT content did not significantly (P > 0.05) change in gills except for 72 h exposure at 30 μg l⁻¹. Nonetheless, the relationship between MT biosynthesis and Mercury bioaccumulation in both tissues was significant (P < 0.05). The results suggest that this form of MT in S. argus was Hg inducible and could be extended as a biomarker of mercury pollution in marine ecosystems.  相似文献   

Genetic analysis of late blight resistance in a new RIL population of tomato derived from LB-resistant Solanum pimpinellifolium accession PI 270443     

Mengyuan Jia  Majid R. Foolad 《Plant Breeding》2020,139(3):651-659

Late blight (LB), caused by Phytophthora infestans, is one of the most devastating diseases of tomato (Solanum lycopersicum) worldwide. Aggressive pathogen isolates resistant to fungicides have driven research in favour of finding new sources of host resistance for tomato breeding. Recently, we reported S. pimpinellifolium accession PI 270443 exhibiting LB resistance stronger than all commercial LB-resistant tomato cultivars. The purpose of this study was to examine the inheritance of LB resistance conferred by this accession. An interspecific cross was made between PI 270443 and a LB-susceptible tomato breeding line and advanced to F₁₀ generation. A total of 166 F₉ and corresponding F₁₀ recombinant inbred lines (RILs) were evaluated for response to LB in four replicated greenhouse experiments. Estimates of heritability (h²) of LB resistance, determined by parent–offspring (F₉:F₁₀) correlation analysis, ranged from 0.66 to 0.81, with an average of 0.76. The moderately high h² of LB resistance in PI 270443 suggests the utility of this accession for tomato breeding. Molecular mapping and RNA-sequencing efforts are underway to identify genes underlying LB resistance in PI 270443.  相似文献   

Surface drainage nitrate loading estimate from agriculture fields and its relationship with landscape metrics in Tajan watershed     

Fatemeh Rajaei  Abbas Esmaili Sari  Abdolrassoul Salmanmahiny  Majid Delavar  Ali Reza Massah Bavani  Raghavan Srinivasan 《Paddy and Water Environment》2017,15(3):541-552

Pasture, forest, and farmland are the dominant land covers in the Tajan River watershed and this landscape status has a direct connection with nitrate pollution. Understanding the correlations between landscape variables and nitrate pollutant is a priority in order to assess pollutants loading and predicting the impact on surface water quality. The soil and water assessment tool was used to simulate nitrate loads in different land cover types in different years. The landscape pattern was calculated by FRAGSTATS. The contributing share of each land use/land cover shows nitrate pollutant produced by grassland (5.7%) and forest (29%) are less than those produced by agricultural land (64.2%). Agricultural land was identified as the main source of nitrate pollution. Paddy fields and orchards had the most intensive soluble nitrate loss especially in spring and summer. Statistical analysis indicated that nitrate was positively associated with patch density, edge density, patch number, total edge, effective mesh size, largest patch index, and landscape shape index (p ≤ 0.01). We then analyzed how nitrate was related to landscape attributes in six different sites. Also the regression analysis results suggested that landscape metrics could account for more than 94% of the variance of nitrate in the whole catchment. The regression models confirmed the great importance of the agriculture metrics and forest metric in predicting nitrate in watershed. Defining the generation and extent of pollution in this particular watershed which discharges into the Caspian Sea can constitute an important step toward protecting this ecosystem.  相似文献   

Numerical modeling based on a finite element method for simulation of flow in furrow irrigation     

Sareh Sayari  Majid Rahimpour  Mohammad Zounemat-Kermani 《Paddy and Water Environment》2017,15(4):879-887

In this study, a zero-inertia finite element model (ZIFEM) is developed and applied for simulating all phases of furrow irrigation based on Saint–Venant equations. The complexity and nonlinear behavior of the Saint–Venant equations are the major difficulty in developing a finite element model to simulate furrow irrigation. Therefore, through the Galerkin FEM approach, the model assesses the free surface flow on a variable cell length at each time step and determines the suitable element length for each individual cell and the model solves the equations by using an iterative method. Along with the free surface flow phase, the infiltration phase is estimated by the Kostiakov–Lewis equation. The ZIFEM model is verified using seven experimental data sets collected from the literature and observed data from the farm consisting of two free drainage furrows with a length of 72 m, a top width of 0.8 m, a depth of 0.25 m and a slope of 0.2%. The model accuracy is studied to simulate advance and recession trajectories and runoff by calculating the root-mean-square error (RMSE), relative error and percentage error. It is observed that in all irrigation events, the proposed model reasonably agreed with field measurements. An evaluation of the RMSE shows that in 81.25% irrigation events the ZIFEM is more accurate than the WinSRFR model. In overall, the results of the model suggest that the ZIFEM can be introduced as a potential numerical tool for analyzing and evaluating furrow irrigation.  相似文献