Dietary organic acid salts mitigate plant protein induced inflammatory response and improve humoral immunity,antioxidative status and digestive enzyme activities in yellowfin seabream,Acanthopagrus latus     

Ebrahim Sotoudeh  Mohammad Sangari  Dara Bagheri  Salim Morammazi  Mansour Torfi Mozanzadeh 《Aquaculture Nutrition》2020,26(5):1669-1680

An eight‐week research was conducted to investigate the effects of single or combined administration of sodium propionate (Na‐P) and sodium acetate (Na‐A) on the performance of yellowfin seabream (Acanthopagrus latus) juveniles (6.5 ± 0.3 g). A plant protein (PP)‐rich diet was supplemented with sole or blends of organic acid salts (OAS) namely Na‐P and Na‐A to design six experimental feeds including control (without OAS), Na‐P5 (5 g/kg Na‐P), Na‐P10 (10 g/kg Na‐P), Na‐A5 (5 g/kg Na‐A), Na‐A10 (10 g/kg Na‐A) and Na‐P + A (5 g/kg Na‐P + 5 g/kg Na‐A). Except for Na‐A5 group, the other OAS‐supplemented treatments had higher growth and feed efficiency ratio than the control (p < .05). The inclusion of OAS in the experimental feeds pronouncedly enhanced plasma lysozyme and alternative complement pathway activities compared to the control. Furthermore, fish fed on the OAS‐supplemented diets had greater catalase and glutathione peroxidase activities in the liver than the control (p < .05). Total antioxidant capacity in the liver of fish fed on the OAS‐supplemented diet also was higher than the control. Fish fed on the OAS‐supplemented diets had higher pepsin, trypsin and lipase activities than the control. The insulin‐like growth factor 1 (IGF‐1) gene expression was remarkably down‐regulated in the liver of fish fed on the OAS‐supplemented diets compared to the control especially in groups fed on the Na‐P10 and Na‐A10 diets. The greatest IGF‐1 gene down‐regulation level in the gut was in fish fed on the Na‐P5 and Na‐P10 diets. The interleukine‐1β in the gut was remarkably up‐regulated in the control compared to the other groups (p < .05). The lactic acid bacterial colonies count in the gut of the control was lower than the OAS‐supplemented groups. Based on the findings of the present study, supplementing PP‐rich diets with 10 g/kg Na‐P or blends of Na‐P (5 g/kg) and Na‐A (5 g/kg) beneficially alleviated inflammatory responses and improved immune parameters and digestive capacity in yellowfin seabream juveniles.  相似文献   

Tissue culture based screening for selection of high biomass and phenolic producing clonal lines of lavender using pseudomonas and azetidine-2-carboxylate.     

H Al-Amier  B M Mansour  N Toaima  R A Korus  K Shetty 《Journal of agricultural and food chemistry》1999,47(7):2937-2943

Lavender is a good source of essential oils and phenolic metabolites for food, medicine, and cosmetic applications. Due to cross-pollination, lavender has substantial plant to plant variation and therefore a high degree of genetic inconsistency in the level of phytochemicals produced for diverse applications. Tissue culture methods, using benzyladenine-induced shoot organogenesis, were used to isolate clonal lines originating from individual heterozygous seeds among a heterogeneous seed population to exploit the genetic heterogeneity. Subsequently, in a two-step method, clonal shoots of each clonal line were evaluated for the ability to tolerate Pseudomonas inoculation and various levels (0-200 microM) of proline analogue, azetidine-2-carboxylate. On the basis of tolerance to Pseudomonas and proline analogue treatments, multiple shoot forming ability, biomass, rosmarinic acid, total phenolics, and total chlorophyll, 20 separate clonal lines were screened and isolated for further vegetative propagation and evaluation. From the clonal lines isolated, lines LH-14, LH-15, LH-17, and LH-11 showed the best potential for overexpression of phenolic metabolites in response to Pseudomonas and proline analogue.  相似文献   

Effect of methanol concentration and thaw rate on the viability and fertility of cryopreserved Arctic char,Salvelinus alpinus (L.), spermatozoa     

Gavin F Richardson  Mary A McNiven  Nabil Mansour 《Aquaculture Research》2011,42(8):1096-1100

In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25 °C water for 17 s (11.6 °C s^?1) or in 5 °C water for 60 s (3.3 °C s^?1). The viability of the frozen–thawed semen was measured by determining post‐thaw motility and sperm membrane integrity. Two fertility trials were also conducted. There was no effect of trial or thaw rate on post‐thaw sperm viability or fertility. Use of 15% methanol in the extender resulted in the highest overall percentage of sperm motility and fertility. Use of 12.5% methanol as a cryoprotectant resulted in a higher per cent post‐thaw motility and a lower percentage of dead cells than did 10% methanol. Thus, levels of methanol higher than the commonly used 10% are beneficial for cryopreserving Arctic char sperm.  相似文献   

Effects of total fish oil replacement to vegetable oils at two dietary lipid levels on the growth,body composition,haemato‐immunological and serum biochemical parameters in caspian brown trout (Salmo trutta caspius Kessler, 1877)     

Abdolmohammad Abedian Kenari  Mansour Torfi Mozanzadeh  Reza Pourgholam 《Aquaculture Research》2011,42(8):1131-1144

This research aimed to evaluate the effects of two dietary fat levels [low fat (LF) (10%), high fat (HF) (20%)] and sources [fish oil (FO), vegetable oil (VO)] on the growth and some physiological parameters of Caspian brown trout fingerlings for 60 days. Tuna oil or blends of canola and soybean oils (85:15) were added to diets to design four feeds namely LFFO, HFFO, LFVO and HFVO according to the fat levels and sources. The fish fed the LFFO diet had lower weight gain than the other fish (P<0.05). The total n‐6 fatty acids increased in fish fed diets with the blends of VO, while the total n‐3 fatty acids decreased in these fish (P<0.05). Serum lysozyme activity was higher in fish fed the HFVO diet than the other fish (P<0.05). Serum glucose, total cholesterol, triglyceride and very low‐density lipoprotein were lower in fish fed LFFO than the other fish (P<0.05). The present study demonstrates that in terms of fish growth, VOs can be used as an alternate source of dietary fat, whereas fish health and nutritional value are improved with the LFFO diet. According to these results, a partial substitution of FO by VO in high‐level fat diets is suggested for long‐term feeding of Caspian brown trout.  相似文献   

Morphological characterization of Arctic char, Salvelinus alpinus, eggs subjected to rapid post-ovulatory aging at 7 °C     

Nabil Mansour  Franz Lahnsteiner  Mary A. McNiven  Gavin F. Richardson 《Aquaculture (Amsterdam, Netherlands)》2008,279(1-4):204-208

Egg quality in Arctic char, Salvelinus alpinus, kept indoors at 7 °C during spawning season was morphologically classified. Four egg categories based on lipid droplet distributions and egg diameter were characterized. Eggs with homogenous lipid vesicle distributions and a uniform size were classified as Good. Eggs with some yolk lipid droplets coalesced toward one pole but were homogeneous in size were classified as Fair. Eggs with lipid vesicles that were usually coalesced at one or two poles with were classified as Poor. The fourth egg category was Heterogeneous, in which lipid vesicle distribution and egg size were inhomogeneous. This distribution pattern of the lipid vesicle had a strong relationship with the percentage of fertilized and eyed eggs. Percentages of fertilized and eyed eggs in Good and Fair eggs were 85.5 and 83.4; 30.2 and 28.2, respectively. With Poor and Heterogeneous eggs, the percentages of fertilized and eyed eggs were zero. Egg diameter and absolute weight was increased in Poor eggs than in other egg categories. Relative weight increase during hardening after 30, 45 and 60 min were higher in Good and Fair eggs than in Poor and Heterogeneous eggs. Ovarian fluid collected from egg batches with low rates of fertilized and eyed eggs (Heterogeneous and Poor) had a lower pH and higher protein and aspartate amino-transferase enzyme than ovarian fluid from eggs batches classified as Good or Fair, while the ovarian fluid osmolality did not significantly differ. ATP contents of Poor and Heterogeneous eggs were lower than for Good and Fair eggs. In Heterogeneous eggs, both ATP and acid phosphatase contents were very variable.  相似文献