Ectomycorrhizal fungi identification in single and pooled root samples: terminal restriction fragment length polymorphism (TRFLP) and morphotyping compared     

David J. Burke  Kendall J. Martin  Mary A. Topa 《Soil biology & biochemistry》2005,37(9):1683-1694

We describe here the results of a study conducted to evaluate a terminal restriction fragment length polymorphism (TRFLP) approach targeting rRNA genes for determination of ectomycorrhizal (ECM) communities colonizing the roots of loblolly pine (Pinus taeda L.). Root tips separated from soil cores were classified according to morphological characteristics and DNA was then extracted from each group of morphotyped tips. Labeled primers were used to generate terminal restriction fragments (TRF) for molecular fingerprinting of root colonizing fungi and to determine how well TRFLP could be used to discriminate between ectomycorrhizal types. Morphotypes generally correlated well with specific TRFs and sequence analysis confirmed that TRFs could be used to discriminate among fungal types. Sequence analysis indicated that important ECM fungi including Russulaceae, Thelephorales, and Tricholomataceae could be fingerprinted with TRFLP. In addition, a fixed proportion of the DNA extracted from each morphotype from the same core was used in a pooling experiment used to assess whether previously identified fungal species types could be distinguished from one another within reconstructed communities. Since some morphotypes share TRFs, dual analysis of ITS1 and ITS2 was necessary for accurate fingerprinting of fungal types. Approximately, 5.0±0.3 phylotypes were detected per core with TRFLP-corrected morphotyping as compared to 4.0±0.4 phylotypes using TRFLP on pooled community samples. TRFLP made on experimental sporocarp communities suggested that reduced ECM richness with TRFLP may be partly caused by differences in the amount of DNA available for PCR and primer bias. Nonetheless, TRFLP on pooled morphotypes accounted for more than 93% of colonized root tips. The method can be used to facilitate analysis of mycorrhizal communities using root tips collected from soil cores.  相似文献   

Untersuchungen zur Entwicklung der apomiktischen Fortpflanzungsweise bei Futtergräsern II. Hybrideffekt und Fertilität von Art- und Gattungsbastarden     

Kurt Gröber  Fritz Matzk  Martin Zacharias 《Genetic Resources and Crop Evolution》1976,24(1):349-364

  相似文献   

Effect of simulated acid rain on mycorrhizal infection of Pinus strobus L.     

H. F. Stroo  Martin Alexander 《Water, air, and soil pollution》1985,25(1):107-114

Mycorrhizal infection but not growth of white pine seedlings was reduced by application of simulated rain at pH 3.5 at 3 times ambient rates to plants grown in steamed Mardin soil inoculated with Pisolithus tinctorius. In unsteamed Mardin soil, the simulated acid rain at 3 times ambient rates had no effect on mycorrhizal infection or growth of pine seedlings if the rain was applied to the plants and soil or to only the soil before planting, except that nitrate-containing acid rain increased growth, infection and N content. In limed Mardin soil, the simulated acid rain reduced mycorrhizal infection if applied to the plants and soil as the seedlings were growing but not if applied to the soil before planting. Application of pH 3.5 rain at ambient rates had no detectable influence on mycorrhizal infection in 6 of 9 soils tested, but it stimulated infestion in 3 soils if the simulated rain contained only sulfate and in 1 soil if it had both sulfate and nitrate.  相似文献   

The persulfate oxidation of fulvic acids     

F. Martin  F. J. Gonzalez-Vila 《植物养料与土壤学杂志》1983,146(4):409-414

Persulfate oxidation of two fulvic acids from different soils was accomplished. This is a new mild chemical degradative method which differentiates between fulvic acids isolated from soils developed under widely differing environments. A fulvic acid isolated from an Humic Haplorthod gives as major oxidation products phenolic acids, whereas a fulvic acid from an Andic Hapludult yields benzenecarboxylic acids as most abundant compounds. The possibility to differentiate between fulvic acids from different soils, as chemical degradation with persulfate does, offers interesting prospects to the characterization of humic materials.  相似文献   

Pyrolysis of a soil humic acid and of their hydrolyzed and methylated products     

F. Martin  F. J. Gonzalez-Vila 《植物养料与土壤学杂志》1983,146(5):653-659

Pyrolysis of soil humic acid yields a variety of products arising from heterogeneous materials associated to the humic structure and from this self. After acid hydrolysis most of the compounds related to proteins, polysaccharides and lignin dissappear. Adsorbed compounds such as fatty and dicarboxylic acids are released after methylation and subsequent pyrolysis. In addition pyrolysis produces aromatic and substituted aromatic hydrocarbons. All the identified pyrolysis products have also been isolated by different complex and time-consuming chemical degradations and solvent extraction procedures. Pyrolysis provide some evidence of the humic acid structure.  相似文献   

Continuous flow fermentation to purify waste water by the removal of cadmium     

R. Campbell  M. H. Martin 《Water, air, and soil pollution》1990,50(3-4):397-408

Fungi have been isolated that can tolerate and absorb high levels of heavy metals, especially Cd, when grown in a continuous flow air-lift fermenter with up to 10 throughputs per day. This allows the recovery of metals or the purification of industrial effluent streams. An average of over 97% of the Cd was removed from the simulated effluent during passage through the fermenter, reducing the Cd concentration from over 6 mg L^?1 to less than 0.2 mg L^?1. The biomass recovered contained up to 2.9% Cd (on a dry weight basis) and can either be disposed of or returned for smelting to recover the metal.  相似文献   

An application of the mixed linear model and the dirichlet process prior in veterinary medicine research     

Abrie J. van der Merwe  Albertus L. Pretorius 《Journal of Agricultural, Biological & Environmental Statistics》2003,8(3):328-343

This article applies Bayesian nonparametric techniques of analysis to the mixed linear model. The distribution of the random effects is specific as a nonparametric prior. A Dirichlet process prior is specified on the space of prior distributions. A modified Dirichlet process is described and applied using a Gibbs sampler. The approach is demonstrated in an investigation of the changes over time of packed cell volume in two breeds of cattle.  相似文献   

Seasonal changes in periphyton nitrogen fixation in a protected tropical wetland     

Rodrigo Vargas  Eberto Novelo 《Biology and Fertility of Soils》2007,43(3):367-372

Cyanobacteria are important for global nitrogen cycle and often form complex associations referred to as cyanobacterial mats or periphyton that are common in tropical, limestone-based wetlands. The objective of this study was to monitor the nitrogen fixation rate using the acetylene reduction assay of these cyanobacterial mats in a tropical, unfertilized, and protected wetland. To account for temporal and spatial variation of nitrogenase activity, we were interested in seasons in a hydrological cycle (dry, rains, and end of rains), sites with different vascular vegetation, and rates of nitrogenase activity in a 24-h cycle. The annual average of nitrogenase activity was 22 nmol C₂H₄ cm⁻² h⁻¹, with a range of <6 to 35 nmol C₂H₄ cm⁻² h⁻¹, and the annual nitrogen fixation rate of our study site (9.0 g N m⁻² year⁻¹) is higher than similar estimates from other freshwater wetlands. There was a clear temporal pattern in nitrogenase activity with a maximum rate occurring during the rainy season (August) and a maximum nitrogenase activity occurring between 0600 and 1200 hours. We found spatial differences in nitrogenase activity among the four sites that could be attributed to variations in species composition within the periphyton.  相似文献   

Nitrogen and carbon mineralization of semi-arid shrubland soil exposed to long-term atmospheric nitrogen deposition     

George L. Vourlitis  Gypsi Zorba 《Biology and Fertility of Soils》2007,43(5):611-615

Anthropogenic N-deposition represents a significant input of N into semi-arid chaparral and coastal sage scrub (CSS) shrublands of southern California. High levels of atmospheric N deposition have the potential to increase soil C and N mineralization, and we hypothesize that semi-arid shrubland soil exposed to long-term (decades) high N deposition will have significantly higher C and N mineralization potentials. This hypothesis was tested in a laboratory incubation where the inorganic N (NH₄+NO₃) and CO₂ production of soils maintained at a constant temperature of 25°C and a soil moisture of 0.25 g H₂O/g (65% water-filled pore space) were sampled sequentially over a 50-week period. The temporal trend in cumulative C and N mineralization was well described by a first- and zero-order model, respectively. Long-term atmospheric N deposition significantly increased potential N mineralization but not C mineralization, and both the rate and total N mineralization were significantly positively correlated with the surface (0–10 cm) soil δ ¹⁵N natural abundance and negatively correlated with the surface soil C:N ratio. While the incubation techniques used here do not provide realistic estimates of in situ C or N mineralization, these assays indicate that atmospheric N deposition has significantly altered ecosystem N storage and cycling.  相似文献   

Changes in soil chemistry associated with the establishment of forest gardens on eroded,acidified grassland soils in Sri Lanka     

Ilyas Siddique  Caroline Gutjahr  Gamini Seneviratne  Broder Breckling  Sudheera W. Ranwala  Ian J. Alexander 《Biology and Fertility of Soils》2007,44(1):163-170

Topsoil properties were determined in forest gardens established about 20 years ago on eroded grassland soils (abandoned tea lands) in the wet zone of the Sri Lankan highlands. They were compared with adjacent, eroded grasslands (abandoned tea lands) on strongly weathered soils vs soils at earlier stages of pedogenic development in a two-way analysis of variance. Soil pH in forest gardens was, on average, 6.1, nearly one unit higher than in the adjacent grasslands. In the garden soils, the cation exchange capacity (CEC measured at pH 4.8) was nearly double, exchangeable calcium concentrations five times and exchangeable magnesium three times as high as in the grasslands soils. Total soil N content was found to be nearly 40% higher in the gardens. Topsoil gravel contents in the gardens were less than half as high as in the grasslands. The increases in exchangeable bases and N in gardens, relative to grasslands, were attributed to increased nutrient retention and acquisition. Higher retention was partly due to the higher CEC_pH4.8, and probably to reduced erosion and increased, continuous fine root density in the garden topsoils. Higher field CEC in gardens was likely to result from generally higher C contents and from the reversal of acidification, presumably caused by base accumulation and decomposition processes. Our results suggest that forest garden establishment on degraded grasslands can lead to accumulation of mobile nutrients in the topsoil, probably due to increased nutrient retention, subsoil uptake and litter input exceeding nutrient uptake by the standing biomass.  相似文献