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排序方式: 共有221条查询结果,搜索用时 15 毫秒
161.
Tani K Une S Hasegawa A Adachi M Kanda N Watanabe S Nakaichi M Taura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):445-448
We demonstrated that Demodex canis was transferred to skin xenografts of a dog and a hamster onto severe combined immunodeficiency mice. After the transfer of mites, the number of eggs, larvae, nymphs and adult mites per gram of canine and hamster xenografts increased, whereas no live mites were detected on murine allograft. These results indicate that D. canis proliferates in hair follicles of dog and hamster skins but not in murine allograft. Therefore, D. canis may have host preference but not strict host-specificity. 相似文献
162.
Eiichi Inoue Masakazu Kasumi Fumio Sakuma Hiroyuki Anzai Katsuki Amano Hiromichi Hara 《Scientia Horticulturae》2006
In Japanese pear, fruit skin color is a very important trait for growers because the russet skin protects the fruit against external stress caused by disease, insects, bad weather, and shipping. This report describes the development of a randomly amplified polymorphic DNA (RAPD) marker linked to major genes controlling the fruit skin color in Japanese pear. Two F1 progenies from the cross of ‘Kousui’ × ‘Kinchaku’ and ‘Niitaka’ × ‘Chikusui’ segregated by fruit skin color were used for bulked segregant analysis. Four kinds of bulked DNA were constructed and used for the polymerase chain reaction in RAPD analysis. After 200 random primers were screened against four bulks, a band named OPH-19425 was selected in cooperation with green bulks. The recombination rate between OPH-19425 and the green skin phenotype was 7.3%. The RAPD marker (OPH-19425) could select green fruit with probability as high as approximately 92%. The marker was apparently useful for the selection of green-skinned individuals in a breeding program. This is the first report on developing a DNA marker closely linked to the fruit phenotype in Japanese pear. 相似文献
163.
Kusuda S Ikoma M Morikaku K Koizumi J Kawaguchi Y Kobayashi K Matsui K Nakamura A Hashikawa H Kobayashi K Ueda M Kaneko M Akikawa T Shibagaki S Doi O 《The Journal of reproduction and development》2007,53(6):1283-1289
The progesterone (P(4)) profiles and macroscopic vulvar changes of female Malayan tapirs were investigated in order to understand their fundamental reproductive physiology and to search for visual indicators of estrus. Blood was collected once or twice a week from seven female Malayan tapirs kept at four zoos. Serum or plasma P(4) concentrations were determined by radioimmunoassay. The P(4) concentrations changed cyclically throughout the years, and a total of 56 cycles was confirmed in the seven females. The length of the estrous cycle based on the P(4) profiles was 43.6+/-2.0 days; however, this mean includes great variation in length, from 21 to 84 days. Mucous discharge from the vulva and vulvar swelling were seen when the P(4) concentrations were low before the beginning of a rise in most cases. In conclusion, captive female Malayan tapirs have variations of approximately 1 to 3 months in estrous cycle length, and visual changes in the vulva are helpful in estimating estrus in female Malayan tapirs. 相似文献
164.
Sakai K Sakabe G Tani O Watanabe Y Jahangir A Nakamura M Takehara K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(12):1307-1311
A field isolate of Newcastle disease virus (NDV) isolated from northern pintail (Anas acuta) in Tohoku district, northeast Japan, was characterized. Phylogenetic analysis of the fusion protein indicated that the isolate belonged to genotype I and was closely related to isolates from the Far East corresponded to the migration route for this bird species. The isolate had the typical avirulent cleavage site of the fusion protein (112)GKQGR*L(117). In addition, pathogenicity tests indicated the isolate to have avirulent characteristics. However, the isolate has been shown to cause fusion cytopathic effects and form plaques on chicken embryo fibroblasts (CEF) in the absence of trypsin. The present results suggest that the CEF-adapted NDV, which is avirulent, is circulating among waterfowl populations. 相似文献
165.
Masaaki Katayama Hiroyuki Igarashi Kenji Tani Hiroaki Kamishina 《Research in veterinary science》2010,89(1):124-180
The effect of fluconazole (Fcz) on the cyclosporine (CsA) dosage was investigated in renal transplanted dogs receiving CsA-based immunosuppressive therapy. Initially, CsA was administered orally twice daily to raise the blood trough level between 400 and 600 ng/ml. After the addition of Fcz, the CsA dosage was adjusted to maintain its therapeutic blood concentration. Fcz significantly decreased CsA dosage in both normal and renal transplanted dogs, but a higher dosage of CsA was needed in renal transplanted dogs. In conclusion, Fcz decreases required CsA dosage and thereby reduces the cost of immunosuppressive therapy in canine renal transplantation. 相似文献
166.
Yamashita A Salam KA Furuta A Matsuda Y Fujita O Tani H Fujita Y Fujimoto Y Ikeda M Kato N Sakamoto N Maekawa S Enomoto N Nakakoshi M Tsubuki M Sekiguchi Y Tsuneda S Akimitsu N Noda N Tanaka J Moriishi K 《Marine drugs》2012,10(4):744-761
Hepatitis C virus (HCV) is a causative agent of acute and chronic hepatitis, leading to the development of hepatic cirrhosis and hepatocellular carcinoma. We prepared extracts from 61 marine organisms and screened them by an in vitro fluorescence assay targeting the viral helicase (NS3), which plays an important role in HCV replication, to identify effective candidates for anti-HCV agents. An ethyl acetate-soluble fraction of the feather star Alloeocomatella polycladia exhibited the strongest inhibition of NS3 helicase activity, with an IC(50) of 11.7 μg/mL. The extract of A. polycladia inhibited interaction between NS3 and RNA but not ATPase of NS3. Furthermore, the replication of the replicons derived from three HCV strains of genotype 1b in cultured cells was suppressed by the extract with an EC(50) value of 23 to 44 μg/mL, which is similar to the IC(50) value of the NS3 helicase assay. The extract did not induce interferon or inhibit cell growth. These results suggest that the unknown compound(s) included in A. polycladia can inhibit HCV replication by suppressing the helicase activity of HCV NS3. This study may present a new approach toward the development of a novel therapy for chronic hepatitis C. 相似文献
167.
Riaz Hasan Keiichiro Nishimura Masakazu Okada Miki Akamatsu Masafumi Inoue Tamio Ueno Tooru Taga 《Pest management science》1996,46(2):105-112
Methyl 3-(4-chlorophenyl)-1-[N-(4-chlorophenyl)carbamoyl]-4-methyl-2-pyrazoline-4-carboxylate was converted to corresponding (1R)- and (1S)-phenethyl esters via its carboxylic acid and acid chloride at the C-4 atom to separate the diastereomers. Their configurations were confirmed by X-ray analysis. Both isomers of the (1R)methylbenzyl ester were subjected to transesterification with sodium methoxide to obtain enantiomers of the starting methyl ester. Their insecticidal activity was measured against American cockroaches (Periplaneta americana (L.)) by injection and against house flies (Musca domestica L.) by topical application under various synergistic conditions with metabolic inhibitors. The activity values of the four α-methylbenzyl esters and the R-isomer of the starting methyl ester were similar. The S-enantiomer of the methyl ester was about 10 and 100 times more active than the R-isomer against the cockroach and the fly, respectively. Some N-arylacetyl and N-aryloxyacetyl derivatives of the starting N-(4-chlorophenyl)carbamoyl compound gave very low activity. Conformation-energy profiles for some compounds suggested that the conformation of substituents on the N-1 atom in the pyrazoline ring has a specific role for the potential insecticidal effects. 相似文献
168.
Kazushi Kusakabe Masaki Hata Jun Shoji Masakazu Hori Takeshi Tomiyama 《Fisheries Science》2017,83(2):215-219
To determine the optimal temperature for juvenile (0 year old) marbled flounder Pseudopleuronectes yokohamae, juveniles of 40–54 mm standard length were reared at six temperature conditions in the range of 8–26 °C, using group- and individual-based methods. Growth of juveniles increased from 8 to 20 °C but decreased from 20 to 26 °C, irrespective of the rearing method used. Food intake was greatest at 20 and 24 °C compared with other temperatures, while feed conversion efficiency was greater at 20 °C than 24 °C in individual rearing. Individual rearing provided more information such as individual variations in growth and food consumption, suggesting the importance of individual-based experiments for exploring the optimal temperature for fish. 相似文献
169.
Shigeki Dan Hiraku Iwasaki Arata Takasugi Shodai Shibasaki Hideki Yamazaki Masakazu Oka Katsuyuki Hamasaki 《Aquaculture Research》2019,50(4):1361-1370
Despite recent advances in culture technology for East Asian common octopus Octopus sinensis paralarvae using upwelling systems, securing suitable feed for the paralarvae is an unresolved issue. The zoea of the swimming crab Portunus trituberculatus is a good candidate for paralarval feed because of the high fecundity of the adult females. To investigate the effects of supplying P. trituberculatus zoeae and their feeding method on paralarvae, we cultured paralarvae with supplying different combination ratios of zoeae and Artemia (10:0, 7:3, 5:5, 3:7 and 0:10), and with or without supplementing rotifers using small‐scale (3‐L) upwelling systems. Paralarval survival rate and growth were improved when zoeae were supplied as the main feed, but reduced when the proportion of Artemia exceeded half the whole preys. Supplementing rotifers did not affect the paralarval survival and growth. Subsequently, paralarvae were cultured by supplying zoeae (partially augmented by Artemia) using three large (1‐kl) upwelling systems to assess their feeding effectiveness in juvenile octopus production. Paralarvae could be cultured at high survival rates of 77.1 ± 5.1% to reach benthic juveniles at 23 days after hatching. In conclusion, supplying P. trituberculatus zoeae augmented with Artemia under an upwelling culture system has great potential for juvenile octopus production. 相似文献
170.
Hirofumi Arai Wataru Tani Akira Okamoto Kenji Fukunaga Yuki Hamada Katsuyasu Tachibana 《Fisheries Science》2009,75(2):499-505
The commercial value of yellowtail Seriola quinqueradiata meat is often reduced by color deterioration of the dark muscle during storage. The objective of this study was to investigate
the effect of simultaneous dietary supplementation of vitamin C (VC) and vitamin E (VE) to cultured yellowtail on color degradation
of the dark muscle during storage. Yellowtail were fed for 6 or 11 days on a diet containing 1% VC and 1% VE (vitamin group)
or without these vitamins (control group). The amounts of VC and VE in the dark muscle of the vitamin group tended to be higher
than those of the control group. During the storage of sliced meat at 23 and 4°C, the decreases in a* value (redness) of the dark muscle of the vitamin group was slower than those of the control group. The formation of metmyoglobin,
which can occur along with myoglobin oxidation, in the dark muscle of the vitamin group was slower than that of the control
group. These results suggest that simultaneous feeding of high amounts of VC and VE for a short period to yellowtail before
harvesting could suppress the color deterioration of the dark muscle during storage. 相似文献