Proliferative responses to canine thyroglobulin of peripheral blood mononuclear cells from hypothyroid dogs     

Tani H  Nabetani T  Sasai K  Baba E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):363-368

The immune responses of hypothyroid dogs to canine thyroglobulin (cTg) were evaluated for the proliferative ability of peripheral blood mononuclear cells (PBMC). PBMC from three hypothyroid dogs with high titers of thyroglobulin autoantibody (TgAA) and 3 clinically normal dogs were cultured with 5, 10, or 20 microg/ml of cTg for 72 hr. The proliferative responses of the cells were determined by the level of incorporated BrdU. The numbers of cells expressing Thy-1, CD4, CD8 and IgG in the PBMC were counted by the immunofluorescence method. Proliferative responses to cTg were observed in the cells from hypothyroid dogs. The number of cells expressing IgG and CD8 in the hypothyroid dogs tended to be high compared with the clinically normal dogs. The CD4+ cells in cultures from hypothyroid dogs increased depending upon the amount of cTg. There was a significant (P<0.05) positive correlation between the number of CD4+ cells and the concentration of cTg in the cultures from hypothyroid dogs. These findings suggest a possible relationship between canine hypothyroidism and cellular immunity. Loss of self tolerance to thyroid antigens in CD4+ T cells may play an important role in the development of canine hypothyroidism.  相似文献   

Genetic diversity of the entomopathogen<Emphasis Type="Italic">Verticillium lecanii</Emphasis> on the basis of vegetative compatibility     

Midori?Sugimoto  Masanori?KoikeEmail author  Hideyuki?Nagao  Kayo?Okumura  Masayuki?Tani  Katsuhisa?Kuramochi 《Phytoparasitica》2003,31(5):450-457

Forty-three isolates ofVerticillium lecanii from insects, phytopathogenic fungi and other substrates were tested for vegetative compatibility by observing heterokaryon formation among complementary nitrate-nonutilizing (nit) mutants.nit mutants were isolated from 42/43 strains examined. Twenty-one isolates were self-incompatible, and the remaining 21 isolates were divided into 14 vegetative compatibility groups (VCGs): ten containing only a single strain each, and the remaining four containing two to four isolates each. Members of isolates in each of these VCGs all shared the same IGS haplotype. Further, the isolates within a VCG were correlated with one another in part by fragment patterns of mt-LrDNA, -SrDNA, Bt-2 and H4 region, by PCR-RFLP and -SSCP, but not by dsRNA. Two isolates belonging to VL-J2 have high virulence to aphids, whereas strains from VL-J1 lack this character. These findings indicate that two VCGs (VL-J1 and -J2) may originate from two distinct clonal lineages. Alternatively, high VCG diversity and HSI frequency ofV. lecanii might be associated with an array of distinct lineages. These data not only suggest relationships among DNA polymorphisms, virulence, and VCG, but also demonstrate genetic heterogeneity ofV. lecanii. http://www.phytoparasitica.org posting Sept. 30, 2003.  相似文献   

Case of Pregnancy in Two Cows with Unicorn Horn of the Uterus either by Artificial Insemination at Ipsilateral or Embryo Transfer at Contralateral Corpus Luteum in the Ovary     

C Moriyama  I Kobayashi  M Tani  T Oishi  M Kajisa  Y Horii  S Kamimura 《Reproduction in domestic animals》2008,43(3):382-384

Two Holstein heifers and a cow were diagnosed with White Heifer Disease by ultrasonography. Case 1 was a 14 month-old heifer with aplasia of both sides of the uterine horn. In case 2, a primiparous cow and case 3, an 18 month-old heifer, both showed aplasia of the right uterine horn. Case 2 became pregnant by artificial insemination at ipsilateral ovulatory follicle and corpus luteum in the left ovary, while case 3 became pregnant by embryo transfer at 7 days after oestrus with contralateral corpus luteum in the right ovary.  相似文献   

Ivermectin treatment of demodicosis in 56 hamsters.     

K Tani  T Iwanaga  K Sonoda  S Hayashiya  M Hayashiya  Y Taura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(11):1245-1247

Fifty-six hamsters with demodicosis were treated with daily oral administration of ivermectin (0.3 mg/kg). Thirty-three cases (58.9%) were cured and 6 cases (10.7%) had improved clinically but needed to continue treatment. Of 5 cases (8.9%) who relapsed within 3 months and were retreated with ivermectin, 4 were cured and 1 needed further treatment. Five cases (8.9%) had improved clinically but died within 3 months. Seven cases (12.5%) had not improved and died within 3 months. Overall 49 (87.5%) hamsters had improved clinically. No significant differences in prognosis of demodicosis were detected according to sex, breed, age and clinical features, but the prognosis of demodicosis in hamsters with concurrent disease was poor.  相似文献   

Differences among six Salmonella serovars in abilities to colonize reproductive organs and to contaminate eggs in laying hens   总被引：3，自引：0，他引：3  

Okamura M  Kamijima Y  Miyamoto T  Tani H  Sasai K  Baba E 《Avian diseases》2001,45(1):61-69

The abilities of Salmonella serovars to colonize the reproductive organs of chickens and to contaminate eggs were compared. Mature laying hens were inoculated intravenously with 10(5) colony-forming units of Salmonella enteritidis, Salmonella typhimurium, Salmonella infantis, Salmonella hadar, Salmonella heidelberg, or Salmonella montevideo to cause the systemic infection. Salmonella enteritidis was recovered from three yolks of the laid eggs (7.0%), suggesting egg contamination from the transovarian transmission of S. enteritidis. The liver, spleen, and cecum were colonized by each serovar similarly at 4 or 7 days postinoculation (PI), whereas the ovary and preovulatory follicles were colonized by S. enteritidis with significantly (P < 0.05) higher levels than by the other serovars at 4 and 7 days PI. Salmonella enteritidis was recovered from the cloaca and vagina at 2, 4, and 7 days PI and from the other portions of the oviduct at 4 and 7 days PI. In addition, S. enteritidis had been persistent in the peripheral blood for 7 days PI. These results suggest that S. enteritidis is the predominant serovar to colonize the reproductive organs of mature laying hens among six serovars used in this study, reflecting the field situatibn in which the predominant outbreaks of human salmonellosis were caused by S. enteritidis-contaminated eggs recently. The ability of S. enteritidis to colonize the reproductive organs may be one of the reasons that egg contamination with S. enteritidis has increased.  相似文献   

Distribution and movement of larvae, juvenile and young of the pointhead flounder Hippoglossoides pinetorum in Ishikari Bay and vicinity, Hokkaido   总被引：1，自引：0，他引：1  

Osamu Tominaga  Masamichi Watanobe  Masakazu Hanyu  Kazuko Domon  Yasuhiro Watanabe and  Toyomi Takahashi 《Fisheries Science》2000,66(3):442-451

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Erratum to: Highly polymorphic nuclear microsatellite markers reveal detailed patterns of genetic variation in natural populations of Yezo spruce in Hokkaido     

Masakazu G. Iwaizumi  Mineaki Aizawa  Atsushi Watanabe  Susumu Goto 《Journal of Forest Research》2015,20(3):364-364

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Effects of dietary lysine/protein ratio and fat levels on growth performance and meat quality of finishing pigs     

Keisuke Maeda  Fumika Yamamoto  Masanari Toyoshi  Masakazu Irie 《Animal Science Journal》2014,85(4):427-434

This study aimed to evaluate the effects of dietary lysine/protein ratio and fat levels on the growth, carcass characteristics and meat quality of finishing pigs fed feed made from food waste, including noodles and chocolate. Four dietary treatments, 2 levels of lysine/protein ratio (0.035 and 0.046) and 2 levels of fat (3.3% and 6.0%), were adapted to a 2 × 2 factorial arrangement. Each diet for the finishing pigs contained the same levels of adequate crude protein (16%) and lysine (0.58–0.75%), and similar levels of high total digestible nutrients (90.2–92.6%). In total, 32 LWD pigs with an average body weight of 57.2 kg were assigned to 4 dietary groups. The pigs were slaughtered at about 115 kg. Growth performance was not influenced by the dietary treatments. Carcass characteristics were slightly influenced by the dietary fat level. As the dietary lysine/protein ratio decreased, the marbling score of Longissimus dorsi muscle increased and the intramuscular fat (IMF) increased from 6.82% to 9.46%. Marbling score was not significantly influenced by the dietary fat level. These results indicate that IMF increased without adverse effects on growth, carcass characteristics and meat quality, when pigs were fed a diet with low lysine/protein ratio.  相似文献   

Microbial biomass in soils with calcium accumulation associated with the application of composted lime-treated sewage sludge     

Masakazu AOYAMA  Baoku ZHOU  Masato SAITOH  Norihiko YAMAGUCHI 《Soil Science and Plant Nutrition》2006,52(2):177-185

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COVER IMAGE     

Hiroyuki Tani  Sena Kurita  Ryo Miyamoto  Kazuhiko Ochiai  Kyoichi Tamura  Makoto Bonkobara 《Veterinary and comparative oncology》2020,18(2)

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