Prevalence of equine herpesvirus type 1 strains of neuropathogenic genotype in a major breeding area of Japan     

Tsujimura K  Oyama T  Katayama Y  Muranaka M  Bannai H  Nemoto M  Yamanaka T  Kondo T  Kato M  Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1663-1667

A single non-synonymous nucleotide substitution of guanine (G) for adenine (A) at position 2254 in the viral DNA polymerase gene (encoded by open reading frame [ORF] 30) of equine herpesvirus type 1 (EHV-1) has been significantly associated with neuropathogenic potential in strains of this virus. To estimate the prevalence of EHV-1 strains with the neuropathogenic genotype (ORF30 G(2254)) in the Hidaka district--a major horse breeding area in Japan--we analyzed the ORF30 genomic region in cases of EHV-1 infection in this area during the years 2001-2010. Of the 113 cases analyzed, 3 (2.7%) were induced by ORF30 G(2254) strains. This prevalence is lower than those observed in the U.S.A. (10.8-19.4%), Argentina (7.4%), France (24%), and Germany (10.6%).  相似文献   

DNA methylation status of bovine blastocyst embryos obtained from various procedures     

Sawai K  Takahashi M  Fujii T  Moriyasu S  Hirayama H  Minamihashi A  Hashizume T  Onoe S 《The Journal of reproduction and development》2011,57(2):236-241

DNA methylation is an important factor for the regulation of gene expression in early embryos. It is well known that the satellite I sequence is more heavily methylated in bovine somatic cell nuclear transfer (NT-SC) embryos than in embryos derived from in vitro fertilization (IVF). However, the methylation status of bovine embryos obtained by other procedures is not well known. To clarify DNA methylation levels of bovine embryos obtained from various procedures, we examined satellite I sequences in bovine blastocyst (BC) embryos derived from NT-SC, NT using embryonic blastomeres (NT-EM), in vivo (Vivo), IVF and parthenogenetic treatment (PA). Furthermore, in order to evaluate the efficacy of DNA demethylation by the NT procedure, we determined the DNA methylation levels in bovine embryos in which NT was recapitulated (Re-NT). Although the DNA methylation levels in the NT-SC embryos were higher than those in the other embryos, the NT-EM embryos exhibited lower DNA methylation levels. The satellite I sequence in the NT-SC embryos was more demethylated than that in the donor cells. Although the DNA methylation level in the individual NT-SC embryos showed variation, the full-term developmental efficacy of these embryos were not different. These findings suggest that the methylation level of the satellite I sequence at the BC stage is not related to the abnormalities of bovine embryos produced by NT-SC. There was no difference in methylation levels between Re-NT and NT-SC embryos. Our results indicated that the DNA methylation status differed among embryos produced by various methods and that at least some of the demethylation of the donor cell genome occurred in the recipient cytoplast after NT-SC, but the demethylation ability of the NT procedure was noted in the first NT but not in the second NT.  相似文献   

Long-term survival of a cow with cervical ectopia cordis     

Onda K  Sugiyama M  Niho K  Sato R  Arai S  Kaneko K  Ito S  Muto M  Suganuma T  Wakao Y  Wada Y 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(6):667-669

This study investigated the long-term survival of a calf with cervical ectopia cordis that grew normally, became pregnant, and calved normally. The cow showed normal cardiac function and absence of peripheral circulation abnormalities. This paper documents antemortem characteristics of the affected cow.  相似文献   

Establishment of a quantitative ELISA for the measurement of allergen-specific IgE in dogs using anti-IgE antibody cross-reactive to mouse and dog IgE     

Okayama T  Matsuno Y  Yasuda N  Tsukui T  Suzuta Y  Koyanagi M  Sakaguchi M  Ishii Y  Olivry T  Masuda K 《Veterinary immunology and immunopathology》2011,139(2-4):99-106

As IgE plays a pivotal role in type I hypersensitivity-mediated allergic diseases, it is valuable to measure absolute quantity of serum antigen-specific IgE for clinical and research purposes. Here we describe a novel ELISA system that enables quantification of antigen-specific IgE in ng/ml in dogs. A newly developed monoclonal antibody (CRE-DM) was shown to recognize canine and mouse IgE equally in a dose dependent manner, but it did not recognize canine IgG. The reactivity of CRE-DM to canine IgE was also confirmed by an inhibition ELISA using canine IgE as an inhibitor and the maximum inhibition rate was 91.3%. In order to know whether canine IgE specific to an allergen could be quantitatively measured with an ELISA using CRE-DM, we established a quantitative ELISA that could measure canine IgE recognizing Cry j 1, one of the major allergens of Japanese cedar pollen. In this ELISA, a standard curve was created by using concentration-predetermined Cry j 1-specific monoclonal mouse IgE. According to the standard curve, the concentration of Cry j 1-specific IgE in dogs that were experimentally sensitized to Japanese cedar pollen could be calculated and determined in ng/ml. The specificity of the Cry j 1-specific IgE ELISA using CRE-DM was also confirmed by inhibition ELISA using canine IgE as an inhibitor and the inhibition rate was 97.0%. Reproducibility of the ELISA in three independent assays was determined using groups of pooled canine sera whose Cry j 1-IgE titers ranged from 155.9 to 888.2 ng/ml. Intra- and inter-assay reproducibility was determined with coefficient of variation ranging between 3.1-5.2% and 2.2-8.0%, respectively. These results demonstrated that the ELISA utilizing CRE-DM was a specific, reliable and robust new laboratory test that could quantify absolute amount of antigen-specific IgE in canine serum. The ELISA will serve as a useful tool in the clinics to evaluate the change of serum IgE titers during anti-allergic treatments as well as during seasonal fluctuation of allergen exposure.  相似文献   

Uptake by dietary exposure and elimination of aflatoxins in muscle and liver of rainbow trout (Oncorhynchus mykiss)     

Nomura H  Ogiso M  Yamashita M  Takaku H  Kimura A  Chikasou M  Nakamura Y  Fujii S  Watai M  Yamada H 《Journal of agricultural and food chemistry》2011,59(9):5150-5158

Uptake and elimination of aflatoxins (AFs) by rainbow trout ( Oncorhynchus mykiss ) during a long-term (21 days) dietary exposure were studied to assess contamination by AFs in aquaculture fish fed AF-containing feed. The uptake factor (UF) of aflatoxin B(1) (AFB(1)) in muscle ranged from 0.40 × 10(-3) to 1.30 × 10(-3). AFB(1) concentrations in liver were 165-342 times higher than in muscle. AFs from feed were more highly accumulated in liver than in muscle. Aflatoxicol (AFL) and aflatoxin M(1) (AFM(1)) were detected in muscle and liver and also in the rearing water. AFL concentrations were higher than AFM(1) by 2 orders of magnitude in muscle, and AFL was a major metabolite of AFB(1). The elimination rate constants (α) of AFB(1) and AFL in muscle (1.83 and 2.02 day(-1), respectively) and liver (1.38 and 2.41 day(-1), respectively) were very large. The elimination half-life (t(1/2)) of AFB(1) was 0.38 days (9.12 h) in muscle and 0.50 days (12.00 h) in liver. The elimination half-life of AFL in muscle and liver was 0.34 day (8.16 h) and 0.29 day (6.96 h), respectively. These data show that AFs are eliminated rapidly and are not biomagnified in fish. Thus, AFB(1) concentration in muscle of fish fed AFB(1)-containing feed (ca. 500 μg/kg) decreased to below the detection limit (20 ng/kg) of the most sensitive analytical method at 1.54 days (36.96 h) after the change to uncontaminated feed.  相似文献   

Effects of oocyte‐derived paracrine factors on release of extracellular vesicles by murine mural granulosa cells in vitro     

Yuta Matsuno  Natsumi Maruyama  Wataru Fujii  Kunihiko Naito  Koji Sugiura 《Animal Science Journal》2020,91(1)

  相似文献   

Efficacy of en bloc thoracic duct ligation in combination with pericardiectomy by video-assisted thoracoscopic surgery for canine idiopathic chylothorax     

Hiroo Kanai DVM  Masaru Furuya DVM  PhD  Ken Hagiwara MD  Aya Nukaya DVM  Motoki Kondo DVM  PhD  Toshihide Aso DVM  Ayako Fujii DVM  Kazumi Sasai DVM  PhD 《Veterinary surgery : VS》2020,49(Z1):O102-O111

  相似文献   

Temporal changes in clinical and radiographic variables in dogs with preclinical myxomatous mitral valve disease: The EPIC study     

Adrian Boswood  Sonya G. Gordon  Jens Häggström  Martin Vanselow  Gerhard Wess  Rebecca L. Stepien  Mark A. Oyama  Bruce W. Keene  John Bonagura  Kristin A. MacDonald  Mark Patteson  Sarah Smith  Philip R. Fox  Karen Sanderson  Richard Woolley  Viktor Szatmári  Pierre Menaut  Whitney M. Church  M. Lynne O'Sullivan  Jean-Philippe Jaudon  Jan-Gerd Kresken  John Rush  Kirstie A. Barrett  Steven L. Rosenthal  Ashley B. Saunders  Ingrid Ljungvall  Michael Deinert  Eric Bomassi  Amara H. Estrada  Maria J. Fernandez Del Palacio  N. Sydney Moise  Jonathan A. Abbott  Yoko Fujii  Alan Spier  Michael W. Luethy  Roberto A. Santilli  Masami Uechi  Anna Tidholm  Christoph Schummer  Philip Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(3):1108-1118

  相似文献   

Preface to the special section “Contamination of Agro-Environment and Forestry with Radionuclides from the Fukushima Daiichi Nuclear Power Station”     

Guest Editor-in-Chief of the Fukushima special section Masanori Saito  Guest Editors of the Fukushima special section Hirofumi Tsukada  Noriko Yamaguchi 《Soil Science and Plant Nutrition》2013,59(6):749-750

  相似文献   

Application of chromatographic method and high-frequency apparatus for determination of K and Na in soil extract     

Hideaki Saeki  Kazuo Fujii 《Soil Science and Plant Nutrition》2013,59(1):190-194

The amount of K and Na in HCI-extract of soils has been determined by various methods, using the flame photometer, and specific reagents, but none of these methods are fully satisfactory from a view point of cost or accuracy; Poul Porter et al.¹⁾ have pointed out the large errors that have frequently occured in tlamephotometry.  相似文献