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251.
L.?BrillanteEmail authorView authors OrcID profile B.?Bois O.?Mathieu J.?Lévêque 《Precision Agriculture》2016,17(6):637-658
Electrical resistivity (ER) can be used to assess soil water in the field. This study investigated the possibility of extending the use of ER to measure plant available soil water variables, i.e. available soil water (ASW), total transpirable SW (TTSW), and fraction of transpirable SW (FTSW) using a pedotransfer approach. In a vineyard, 224 electrical resistivity tomography (ERT) transects and 672 time domain reflectometry (TDR) soil water profiles were acquired over 2 years. Soil physical–chemical properties were measured on 73 soil samples from eight different sites. To estimate the amount of soil water available to plants, grapevine (Vitis vinifera L.) water status was monitored by means of leaf water potentials. A benchmark experiment was carried out to compare four machine-learning techniques: multivariate adaptive regression splines (MARS), k-nearest neighbours (KNN), random forest (RF), and gradient boosting machine (GBM). Model interpretation led to a deeper understanding of the relationships between electrical resistivity and soil properties when predicting soil water availability for the plant. The models assessed had good predictive performance and were therefore used to map ASW, TTSW and FTSW in the vineyard. ER coupled to machine-learning algorithms was shown to be a good proxy for quantification and visualisation of plant available soil water with low disturbance. 相似文献
252.
Pasquet V Morisset P Ihammouine S Chepied A Aumailley L Berard JB Serive B Kaas R Lanneluc I Thiery V Lafferriere M Piot JM Patrice T Cadoret JP Picot L 《Marine drugs》2011,9(5):819-831
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL−1 (0.17 μM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL−1 (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL−1 (66.7 μM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained. 相似文献
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254.
Ammar?ShihanEmail author Stephan?H?ttenschwiler Alexandru?Milcu Fran?ois-Xavier?Joly Mathieu?Santonja Nathalie?Fromin 《Biology and Fertility of Soils》2017,53(2):171-185
This study aimed at quantifying the consequences of reduced precipitation and plant diversity on soil microbial community functioning in a Mediterranean shrubland of southern France. Across a natural gradient of shrub species diversity, we established a total of 92 plots (4 × 4 m) with and without a moderate rain exclusion treatment of about 12 % of total precipitation. Shrub diversity included all possible combinations of the four dominant species (Cistus albidus, Quercus coccifera, Rosmarinus officinalis, and Ulex parviflorus). Respective leaf litter mixtures of these species combinations were exposed in all plots over 2 years. We quantified how litter species richness and the reduction in precipitation affected the soil microbial substrate utilization (measured by CO2 evolution using the MicroResp method) on soil samples collected underneath each individual litter mixture after 1 and 2 years of decomposition. Moderate precipitation reduction had a minor impact, but litter species richness and the dissimilarity in phenolic concentrations (estimated using Rao’s quadratic entropy) showed a positive effect on the diversity of substrates metabolized by the microbial communities. Moreover, litter species richness increased soil microbial activity by increasing the catabolic diversity of the soil microbial community. These effects were mostly driven by the presence of Quercus and Ulex leaf litter, which at the same time reduced microbial metabolic dominance, while the presence of Rosmarinus had opposite effects. Our data suggest that plant species loss can have stronger effects on the functioning of soil microbial communities than moderate drought, with potentially important feedbacks on biogeochemical cycling in Mediterranean shrubland ecosystems. 相似文献
255.