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A study was conducted to evaluate the probiotic effect of Pediococcus acidilactici MA18/5M on rainbow trout, Oncorhynchus mykiss. Fish (310 ± 9 g) were fed a control diet or a P. acidilactici‐supplemented diet (at 2.4 × 106 CFU/g) for 4 weeks. The probiotic was observed to populate the intestine with levels ranging from log 3.7 to 5.4 CFU/g. Furthermore, these populations were able to persist for at least 24 hr after the cessation of probiotic feeding. High‐throughput sequencing analysis of bacterial 16S rRNA libraries demonstrated that P. acidilactici was able to modulate the gut microbiome of rainbow trout and that the probiotic was detected as a common taxon on the mucosa and in the digesta of the probiotic fish (p < .05). Real‐time polymerase chain reaction demonstrated that feeding the probiotic upregulated pro‐inflammatory cytokines, interleukin‐1β, and interleukin‐8 and downregulated anti‐inflammatory interleukin‐10 compared to the control‐fed fish. Furthermore, the mRNA levels for the mucosal antibody immunoglobulin T was also elevated in probiotic‐fed fish. These findings help to explain some of the mechanisms behind the previously reported observed benefits of using this probiotic in the intestinal morphology and immunity of rainbow trout.  相似文献   
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The larvicidal and ovicidal effectiveness of twelve insecticides on Grapholita lobarzewskii Nowicki was tested by dipping apples in different concentrations of the insecticides. Emamectin was the most effective larvicidal product, with an LC(50) of 0.01 mg kg(-1), closely followed by spinosad, methoxyfenozide and chlorpyrifos-methyl, with LC(50) values between 0.2 and 0.7 mg kg(-1). Products like imidacloprid, indoxacarb, phosmet and thiacloprid gave LC(50) values between 1 and 2 mg kg(-1). Lastly, chlorpyrifos-ethyl and phosalone had LC(50) values of around 4 mg kg(-1), whereas tebufenozide and diflubenzuron had almost no larvicidal effect. Most of the products tested had low ovicidal effectiveness, and only fenoxycarb and emamectin gave LC(50) values worthy of note, close to 2 mg kg(-1).  相似文献   
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The soybean isoflavones, daidzein, genistein, and glycitein, were hypothesized to act as cholesterol-lowering components, separate from soy protein. Pure synthetic daidzein, genistein, or glycitein (0.9 mmol/kg diet) or a casein-based control diet was fed to groups of 10 female Golden Syrian hamsters for 4 weeks. Hamsters fed glycitein had significantly lower plasma total (by 15%) and non-HDL (by 24%) cholesterol compared with those fed casein (P<0.05). Daidzein and genistein's effects on these lipids did not differ from the effects of either casein or glycitein. Plasma HDL cholesterol and triglyceride concentrations were not significantly affected by dietary treatments. The percentage of urinary recovery of the ingested dose of each isoflavone was glycitein>daidzein>genistein (33.2%, 4.6%, 2.2%, respectively), with the apparent absorption of glycitein significantly greater than that of the other isoflavones. These data suggest that glycitein's greater cholesterol-lowering effect was due to its greater bioavailability, as reflected in its urinary recovery compared with that of the other isoflavones.  相似文献   
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A survey on the occurrence on grape of fungi species in 2001 and their capability to produce ochratoxin A (OTA) and naphtho-gamma-pyrones (NGPs) was conducted in different vineyards from several French viticulture regions. The total numbers of fungal isolates, from setting to harvest, were 732. The Aspergillus genus was essentially represented by section Nigri (98.53%) and it was predominant (74.72%) when compared to Penicillium (25.27%). Approximately one third (30.46%) of the fungal isolates were OTA producers, and 94.17% belong to black aspergilli; Aspergillus carbonarius was the main OTA producer. Moreover, 8.33% of isolates (belong to A. carbonarius and A. niger) were NGP producers. However, none of the Penicillium spp. or other Aspergillus spp. isolates can produces NGP derivatives under the conditions used. No other study on NGPs production by fungi isolated from grapes has been reported. In the second part, a novel NGP, named aurasperone G (1), was isolated from the fermentation broth of the culture extracts of Aspergillus niger C-433, strain producer of OTA, along with the known compound aurasperone F (2). The chemical structure of the new polyketide was proposed based on complete (1)H and partial (13)C, COSY, HMQC, 1D NOE NMR spectra as well as UV and MS spectra. This new NGP was not reported before in nature or prepared synthetically.  相似文献   
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BACKGROUND: Bifora radians, an annual weed in winter wheat, is distributed mainly in the Mediterranean area, Asia Minor and the Caucasus. It infests winter-sown crops of the Central Anatolia and Middle Black Sea regions of Turkey. Field experiments in heavily B. radians-infested fields were conducted over 3 years in Samsun, Turkey, to determine the response of B. radians to ALS-inhibiting herbicides, because growers had complained of a decrease in herbicide effect. RESULTS: The efficacy of ALS inhibitors on a putatively tolerant population sprayed annually with ALS inhibitors and an adjacent allegedly sensitive population was estimated at the ED50 and ED90 response levels. The recommended rates of herbicides controlled 90% of the weed (ED90) in the sensitive population at the early stage of B. radians development, but not in the tolerant population. The relative potencies (EDx(tolerant)/EDx(sensitive)) of herbicides on the two populations were estimated by assuming years as being random effects. The relative potency was on average about 1.7, irrespective of the EDx levels. CONCLUSION: Although the relative potencies were not large, they were large enough to be noted by growers. In field experiments it would be important to establish tools demonstrating when farmers recognise loss of herbicide efficacy. There has been no indication of evolution of resistant biotypes so far, but continuous spraying favours biotypes with increased levels of tolerance. Copyright © 2011 Society of Chemical Industry  相似文献   
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The aim of this study was to compare the influence of a high α-linolenic acid (ALA) diet (linseed diet) and a high linoleic acid (LA) diet (sunflower diet) on performances of pigs, on the dietetical quality of their tissues (adipose tissues and muscles), and on the lipogenic potential of these tissues. Growth and carcass performances, and the lipid content of the tissues were not affected by the diet. Feeding the linseed diet increased the content of n-3 PUFA and decreased the LA/ALA ratios in all the tissues, while the sunflower diet led to an increase in the n-6 PUFA contents. Neither the stearoyl-CoA-desaturase and glucose-6-phosphate-dehydrogenase activities nor the β-hydroxyacyl-CoA-dehydrogenase activity were affected by the diet. Acetyl-CoA-carboxylase and fatty acid synthase activities were enhanced with the linseed diet in respectively subcutaneous adipose tissue and intermuscular adipose tissue, while malic enzyme activity was decreased in liver and subcutaneous adipose tissue of pigs fed the linseed diet.  相似文献   
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This work focuses on a new approach to quantify the effects of above-ground earthworm's activity on soil erosion in steep slope ecosystems such as in Northern Vietnam. In these areas and in many others in the world, soil erosion becomes a major issue while the factors that determine it are still misunderstood. Earthworm's activity is believed to influence soil erosion rate, but we are still unable to precisely quantify their contribution to soil erosion. In this study, we used Near Infrared Reflectance Spectroscopy (NIRS) to quantify the proportion of soil aggregate in eroded soil coming from earthworm activity. This was done by generating NIRS signatures corresponding to different soil surface aggregates (above-ground soil casts produced by earthworms vs. surrounding topsoil).  相似文献   
60.
A reversible taurine-deficient dilated cardiomyopathy occurred in five related golden retrievers. An apical systolic heart murmur was the most common physical abnormality. According to fractional shortening and end-systolic diameter on echocardiography, significant improvements (P<0.005) were recorded within 3 to 6 months of starting taurine supplementation. The dogs regained substantial systolic function, and four were weaned off all cardiac medications except taurine. This response to therapy was unusual, because canine dilated cardiomyopathy is generally progressive and fatal.  相似文献   
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