An experiment was conducted to evaluate the effects of different proportions of ‘Au Grazer’ sericea lespedeza [SL, Lespedeza cuneata (Dum. Cours.) G. Don], a legume rich in condensed tannins (CT), on nutrient intake and digestibility, and to estimate methane (CH4) emissions and 13C isotopic composition (δ13CCH4) from beef steers consuming a forage-based diet. Twenty-five Angus-crossbred steers were distributed in a randomized complete block design (344 ± 48 kg initial BW), and randomly assigned to one of five treatments: 0SL, 25SL, 50SL, 75SL, and 100SL, diets containing 0%, 25%, 50%, 75%, and 100% of SL hay, respectively, mixed with ‘Tifton-85’ bermudagrass hay (Cynodon spp.). The study was carried out for two experimental periods of 21-d each. The statistical model included the fixed effect of treatment and random effects of block, experimental period, and their interaction. Apparent total tract digestibility of crude protein, neutral detergent fiber, and acid detergent fiber was linearly decreased (P < 0.001) by the inclusion of SL. No effects were observed for total CH4 emissions per day, nor for CH4 relative to organic matter intake or digestible organic matter with the inclusion of SL. However, emission of CH4 in relation to intake of CT was affected by treatment (P < 0.001). A linear (P < 0.001) decrease and a quadratic effect (P < 0.001) were observed for δ13C of diets and gas, respectively, in which diets and enteric CH4 with greater inclusion of SL were more depleted in 13C. Moreover, the difference in δ13C between diets and gas (Δδ13C) had a linear decrease (P = 0.001) with the inclusion of SL. The model developed to predict the C3 proportions in the enteric CH4 fitted to predicted values (P < 0.0001). Therefore, greater proportions of SL resulted in lesser CH4 emission when CT intake was considered and the isotopic composition from enteric CH4 was able to predict the contribution of SL in the emissions. 相似文献
Ecuadorian Penaeus vannamei were cultured in dirt ponds (each of approximately 163 m2) at four different stocking densities, i.e. 5 shrimp m−2, 10 shrimp m−2, 15 shrimp m−2 and 20 shrimp m−2. Experiments were carried out over three different periods during the year. Each experiment lasted for 11–14 weeks. No commercial feed was given to the shrimp. The only input to the ponds was about 30 kg of cattle manure per pond per week. Chemical composition of the cattle manure was analyzed. Water quality parameters such as temperature, pH, DO and turbidity were recorded twice daily for each experiment; nutrients (nitrite, nitrate, ammonium and phosphate), water ATP, sediment ATP, H2S and chlorophyll were measured twice weekly for each experiment. Shrimp were sampled either weekly or bi-weekly for body weight measurements.
The results showed a negative correlation between stocking density and growth. Weekly growth ranged from 0·44 to 1·58 g week−1. Survival was over 50% in all treatments and averaged at 70·8%. Under these stocking densities, shrimp production ranged from 4·4 to 18·8 kg ha−1 day−1. The stocking density of 15 shrimps m−2 provides better production than the other stocking densities.
Water quality data did not relate to any shrimp growth. Water nutrient levels in pond discharge water were less than or equal to the nutrients in the incoming water in spite of the weekly addition of cattle manure and did not increase with the addition of cattle manure. No coliform bacteria were detected in any pond water samples through the study period. This indicates digestion of cattle manure in marine shrimp ponds would not pollute the environment with high concentrations of dissolved nutrients.
Thus, a marine shrimp pond can be considered a dissolved nutrient marine treatment plant converting unwanted cattle manure (1841 kg cattle manure ha−1 week−1 in this study) into a valuable commodity — shrimp. 相似文献
The amount of short-wave solar radiation willingly tolerated by lactating Holstein cows on pasture was determined in an equatorial semi-arid environment. The study was carried out on a dairy farm located in Limoeiro do Norte, CE, northeastern Brazil. The observed behaviours were as follows: grazing, under the sun, under the shade, standing, lying, ruminating, idling and wallowing in the water. The behaviours were recorded using instantaneous scan sampling at regular intervals of 15 min from 0600 to 1800 hours over 5 days. On all sampling days, the meteorological variables, including local short-wave solar radiation (RS-W, W m?2), were recorded. The RS-W data were divided into five levels. The sun exposure was more frequent under low (100 %) and moderately low (97 %) levels, when RS-W remained below 500 W m?2. The grazing was more intense under low (100 %) and moderately low (93 %) levels. Above 500 W m?2, the grazing time significantly decreased (11 %). The cows avoided grazing under high (0 %) and very high (0 %) levels, when RS-W exceeded 700 W m?2. The ruminating behaviour was more frequent under high (33 %) and very high (37 %) levels, in which the highest averages of RS-W were recorded (815 and 958 W m?2, respectively). The standing posture was more frequent under low (100 %) and moderately low (97 %) levels. Therefore, the critical RS-W level that motivates cows to stop grazing and seek shade was in the interval between 500 and 700 W m?2. 相似文献
The use of native and adapted forages is an alternative for sustainable production systems in northeast Brazil. The goal of this study was to evaluate the substitution of Tifton 85 by maniçoba hay on the performance and carcass traits of Morada Nova hair sheep. The 16 lambs that were used in this study initially weighed 19.36?±?1.48 kg, were housed in individual pens, and were slaughtered at 58 days of confinement. The dry matter intake (839.84 g/day and 3.81 % WB) was higher (P?<?0.05) in sheep fed maniçoba hay. The feed conversion was 7.39 for sheep fed maniçoba hay. The empty body weight (21.61 kg) and cold carcass weight (11.40 kg) did not differ (P?>?0.05) between sheep fed different hay types. The replacement of Tifton 85 by maniçoba hay did not influence the loin eye area (cm2) or carcass compactness index (kg/cm). The replacement of Tifton 85 by maniçoba hay in Morada Nova sheep diet did not affect the weight gain or carcass characteristics. The maniçoba hay can be considered a forage resource for feeding sheep in Brazilian semiarid tropics. 相似文献
A 2.5‐year‐old intact male Roborovski hamster (Phodopus roborovskii) was presented with a large subcutaneous mass overlying the abdomen, affecting the animal's ambulation and access to different compartments of the cage through narrow tubing. Ultrasound examination delineated a well‐circumscribed mass in the subcutis of the caudoventral abdominal region. The mass was surgically excised and on cytologic examination showed, in a background of blood, a small population of individually arranged oval to spindle‐shaped cells that exhibited a moderate degree of anisokaryosis, coarsely stippled chromatin, one or more prominent nucleoli, and lightly basophilic well‐defined cytoplasmic processes. Histologically, the mass was composed of interlacing streams and bundles of pleomorphic spindle cells (ganglion‐like cells) with variable amounts of collagenous stroma. The neoplastic cells exhibited moderate features of malignancy. These cells stained intensely with vimentin, but not with any other markers, including antibodies to cytokeratin AE1/AE3, S100 protein, desmin, smooth muscle actin, synaptophysin, neurofilament, and androgen receptor. Based on histologic features, the mass was diagnosed as an atypical fibrosarcoma. This is the first report of an atypical fibrosarcoma in a Roborovski hamster and one of few reports of atypical fibrosarcoma in domesticated hamsters overall. 相似文献
The stifle is a large and complex joint in quadrupeds, including horses, making the diagnosis of stifle diseases a challenge for the clinician. The detection of “stifle syndrome” during acupuncture examination of the equine stifle may be useful for diagnosis of stifle diseases in horses. The aim of the present study was to describe stifle syndrome diagnosis in horses. From 2001 through 2012, 810 athletic horses involved in different disciplines were examined using the technique of acupuncture diagnosis. Stifle syndrome was suspected when the following points were reactive: the sacral point suggestive of stifle disease located at the sacral origin of the biceps femoris muscle, bladder (BL)-20, BL-21, and stomach (ST)-30, together with the three local points in the muscle groove of the posterolateral aspect of the thigh. The diagnostic protocol included radiography and/or ultrasonography in certain cases. The horses were evaluated because of reduced athletic performance, routine or prepurchase examination, hind limb lameness, refusal to jump, inappetence, upward fixation of the patella, thoracolumbar spine or sacroiliac pain, suspensory lesion, and front hoof problems, and 86 (9.4%) were diagnosed with stifle syndrome. The sacral acupuncture point suggestive of stifle syndrome correlated with BL-21, local stifle points, gall bladder (GB)-27, spleen-13, BL-23, BL-52, BL-26, and/or GB-25, ST-30, BL-54 (P < .0001), and BL-20 (P = .003). Twenty-two (25.6%) of the 86 horses with stifle syndrome underwent ultrasonography and/or radiography, and lesions were detected in 21. In conclusion, acupuncture for diagnosis of stifle disease may be considered a diagnostic method to be included in routine clinical examination of horses, and its reproducibility shall be investigated in further studies. 相似文献
Loperamide is a peripheral
opiate agonist that can cause apoptosis and G2/M arrest in human cancer cell lines and may
sensitize cells to chemotherapy. The objectives of this study were to investigate the
effects of loperamide on viability, apoptosis and cell cycle kinetics in canine cancer
cells and to establish whether the drug sensitizes cells to doxorubicin. Cell viability
was assessed using Alamar Blue. Cell death and cell cycle were studied using flow
cytometry with 7-Aminoactinomycin-D (7-AAD) and propidium iodide (PI), respectively.
Loperamide decreased cell viability in a dose-dependent fashion and was most effective
against canine osteosarcoma cells. In all cell lines, it induced a dose and time dependent
apoptosis and resulted in accumulation in G0/G1. When co-incubated with doxorubicin,
loperamide induced a synergistic cell kill in canine carcinoma cells. Investigation is
warranted into the role of loperamide in the treatment of canine cancer. 相似文献
The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle‐stimulating hormone (FSH) i.m. in six descending doses at 12‐h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate‐buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick‐end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E2) and progesterone (P4) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p < 0.005), fewer embryos classified as degenerated (p < 0.001) and fewer TUNEL‐positive cells/blastocyst (p < 0.001). The number of transferable embryos was positively correlated with E2 concentrations on day of oestrus (r = 0.562; p < 0.01) and P4 concentrations on the day of embryo collection (r = 0.912; p < 0.001). We concluded that in vivo leptin administration during FSH treatment improved embryo quality and affected ovarian steroidogenesis in superovulated goats. 相似文献