Ultrastructural features of alveolar lesions in induced respiratory syncytial virus pneumonia of calves   总被引：2，自引：0，他引：2  

D G Bryson  S McConnell  M McAliskey  M S McNulty 《Veterinary pathology》1991,28(4):286-292

Ultrastructural changes occurred in alveolar epithelium in the acute and repair stages of induced respiratory syncytial virus pneumonia induced in eight calves (calf Nos. 1-7, 3 to 6 days old and calf No. 8, 2 weeks old), using a bovine strain of respiratory syncytial virus. Five of the calves were Friesians, three were Hereford x Friesians, and all were male. Tissues from three mock-infected control calves (two Friesian, one Hereford x Friesian) were also examined. Evidence of respiratory syncytial virus infection was observed in both type I and type II pneumocytes from day 4 to day 8 after infection. Infection of type I pneumocytes frequently resulted in necrosis. The response of type II pneumocytes to respiratory syncytial virus infection varied and included hypertrophy, hyperplasia, and syncytial formation. In some infected type II pneumocytes, there were numerous irregular projections of the cell surface, associated with viral budding. Hypertrophy and hyperplasia of type II pneumocytes, epithelial syncytium formation, and irregular cytoplasmic projections from epithelial cells caused considerable thickening of respiratory membrane and occlusion of alveolar lumina. Neutrophils were frequently observed in close association with virus-infected epithelial cells, but evidence of respiratory syncytial virus infection and replication was not observed in alveolar macrophages or neutrophils. Proliferation of type II pneumocytes appeared to play a major role in maintaining the integrity of the alveolar epithelium during the acute stage of the experimental pneumonia. Increased numbers of type II pneumocytes were present on alveolar walls, particularly from 4 to 8 days after infection, and some alveoli were lined entirely by this cell type. In some areas, however, squamous epithelial cells were also involved in covering exposed alveolar basement membrane.  相似文献   

Client knowledge,attitudes and practices regarding zoonoses: a metropolitan experience          下载免费PDF全文

SG Steele  SM Mor 《Australian veterinary journal》2015,93(12):439-444

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非点源污染模型在土壤侵蚀模拟中的应用及发展动态   总被引：10，自引：0，他引：10  

牛志明  解明曙  孙阁 《北京林业大学学报》2001,23(2):78-84

近年来 ,随着计算机和地理信息技术的不断发展 ,非点源污染模型在土壤侵蚀研究中得到了广泛的应用 .该文通过对不同模型的背景、功能、应用范围以及优缺点的分析 ,对国外较为常用的 8个土壤侵蚀模型进行了全面综述 .这些模型包括用于预测预报的RUSLE ,WEPP和EPIC ,用于流域水文过程模拟的SHE ,CREAMS和ANSWERS等 ,还有用于流域管理措施评价的AGNPS和SWAT等 .通过对不同模型应用现状的分析 ,提出了土壤侵蚀模拟模型在BMPs评价、湿地水文以及生态系统缓冲带泥沙及营养元素运移等领域的应用前景和发展动态  相似文献   

Validation of an efficient visual method for estimating leaf area index in clonal Eucalyptus plantations     

Rodrigo Hakamada  Carmeni Giunti Neto  Cristiane CZ de Lemos  Sérgio R Silva  Marina SG Otto  Kevin B Hall 《Southern Forests》2016,78(4):275-281

Leaf area index (LAI) is a key ecophysiological parameter in forest stands because it characterises the interface between atmospheric processes and plant physiology. Several indirect methods for estimating LAI have been developed. However, these methods have limitations that can affect the estimates. This study aimed to evaluate the accuracy and applicability of a visual method for estimating LAI in clonal Eucalyptus grandis × E. urophylla plantations and to compare it with hemispherical photography, ceptometer and LAI-2000^® estimates. Destructive sampling for direct determination of the actual LAI was performed in 22 plots at two geographical locations in Brazil. Actual LAI values were then used to develop a field guide with photographic images representing an LAI range of 1.0–5.0 m² m^?2 (leaf area/ground area). The visual LAI estimation guide was evaluated with 17 observers in the field. The average difference between actual LAI and visual LAI estimation was 12% and the absolute difference between the two methods was less than or equal to 0.5 m² m^?2 in 77% of plots. Pearson’s correlation coefficients were high between actual LAI and hemispherical photographs (0.8), visual estimation (0.93) and LAI-2000^® (0.99) and low for the ceptometer (0.18). However, absolute values differed among methods, with the average difference between the actual and estimated LAI of [12]% for visual estimation, 28% for the LAI-2000^®, 37% for the ceptometer and ?43% for hemispherical photographs. The LAI-2000^® and ceptometer overestimated LAI in all plots, whereas hemispherical photographs underestimated the values in all measurements, showing that these methods need calibration to be used. No differences were observed between actual LAI and visual estimates across stand ages of 2–8 years and LAI of 1.5–5.3 m² m^?2 (P > 0.05). The results show that visual estimation of LAI in Eucalyptus stands is a practical method that is unaffected by atmospheric characteristics and can be used on an operational scale.  相似文献   

Clinical,Ultrasonographic and Pathological Findings in a Bull with Segmental Aplasia of the Mesonephric Duct     

HJ Williams  SG Revell  SFE Scholes  AE Courtenay  RF Smith 《Reproduction in domestic animals》2010,45(5):e212-e216

On assessment for use in an AI stud, a 12‐month‐old bull was found to produce low volume ejaculates with 41% of the sperm having morphological abnormalities. No left epididymal tail was palpable and the head of the epididymis on the left was twice the size compared with the right. Ultrasound examination showed the left testis to contain a large central area of decreased echogenicity, which could be followed proximally to a 15‐mm echolucent lesion at the site of the epididymal head. Postmortem examination revealed a 15‐mm diameter cyst in the region of the left epididymal head, and absence of the body and tail of the epididymis. The mediastinum testis of the left testis was dilated, corresponding to the area of decreased echogenicity observed on ultrasonography. No left seminal vesicle was present and the ampulla was significantly smaller than the same structure on the right. Histological examination revealed incomplete or absent spermatogenesis involving the majority of seminiferous tubules in the left testis, and a small proportion of those of the right testis. The cystic structure at the site of the left epididymal head was lined by irregular, sometimes attenuated, epithelium and contained sparse spermatozoa. This case demonstrates the adverse impact, which segmental aplasia of the mesonephric duct had on the testicular and epididymal function of a bull, and highlights the importance of careful clinical assessment in its diagnosis.  相似文献   

Hydrogen radicals, nitrogen radicals, and the production of O3 in the upper troposphere     

PO Wennberg  TF Hanisco  L Jaegle  DJ Jacob  EJ Hintsa  EJ Lanzendorf  JG Anderson  R Gao  ER Keim  SG Donnelly  LAD Negro  DW Fahey  SA McKeen  RJ Salawitch  CR Webster  RD May  RL Herman  MH Proffitt  JJ Margitan  EL Atlas  SM Schauffler  F Flocke  CT McElroy  TP Bui 《Science (New York, N.Y.)》1998,279(5347):49-53

The concentrations of the hydrogen radicals OH and HO2 in the middle and upper troposphere were measured simultaneously with those of NO, O3, CO, H2O, CH4, non-methane hydrocarbons, and with the ultraviolet and visible radiation field. The data allow a direct examination of the processes that produce O3 in this region of the atmosphere. Comparison of the measured concentrations of OH and HO2 with calculations based on their production from water vapor, ozone, and methane demonstrate that these sources are insufficient to explain the observed radical concentrations in the upper troposphere. The photolysis of carbonyl and peroxide compounds transported to this region from the lower troposphere may provide the source of HOx required to sustain the measured abundances of these radical species. The mechanism by which NO affects the production of O3 is also illustrated by the measurements. In the upper tropospheric air masses sampled, the production rate for ozone (determined from the measured concentrations of HO2 and NO) is calculated to be about 1 part per billion by volume each day. This production rate is faster than previously thought and implies that anthropogenic activities that add NO to the upper troposphere, such as biomass burning and aviation, will lead to production of more O3 than expected.  相似文献   

A contagious pox disease in monkeys   总被引：1，自引：0，他引：1  

A S Hall  W P McNulty 《Journal of the American Veterinary Medical Association》1967,151(7):833-838

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RADIOGRAPHIC EVALUATION OF APPENDICULAR SKELETAL MATURATION IN THE RHESUS MONKEY*     

S. Silverman  J. P. Morgan  R. Ferron  W. McNulty  D. Merten 《Veterinary radiology & ultrasound》1983,24(1):25-34

Longitudinal radiographic evaluation of skeletal maturation in 28 rhesus monkeys demonstrated that the majority of appendicular ossification centers were identified radiographically by 175 days of age and that physeal union was complete by 7.3 years in males and 7.2 years in females.  相似文献   

Demonstration of bovine virus diarrhoea virus antigen in formalin fixed, paraffin embedded tissue using a streptavidin/biotin technique     

G M Allan  M S McNulty  D Bryson  D Mackie  M Platten 《Research in veterinary science》1989,46(3):416-418

The detection of bovine virus diarrhoea virus (BVDV) antigen in sections from formalin fixed, paraffin embedded tissue is described. Pre-digestion of the sections with 0.02 per cent protease XIV for 18 hours at 4 degrees C is necessary to unmask formalin fixed antigen. A hyperimmune antiserum prepared in a pig, using a combination of BVDV and hog cholera virus inoculations, linked to a biotinylated anti-pig/streptavidin peroxidase detection system demonstrated antigen in a wide range of tissues from cases of mucosal disease and persistently viraemic animals. The inclusion of a monoclonal anti-pig immunoglobulin linked to a biotinylated anti-mouse/streptavidin peroxidase detection system greatly reduced non-specific staining.  相似文献   

Studies on rotavirus infection and diarrhoea in young calves.     

M S McNulty  J B McFerran  D G Bryson  E F Logan  W L Curran 《The Veterinary record》1976,99(12):229-230

The occurrence of diarrhoea in calves was monitored during the first three weeks of life. Calves fed amounts of colostrum sufficient to produce serum Ig levels in excess of 30 mg per ml did not develop diarrhoea, whereas calves fed less colostrum did. Rotaviruses and mycoplasma-like particles were observed in the faeces of calves with and without diarrhoea. The epidemiology of rotavirus infection in calves is discussed.  相似文献